FastQCFastQC Report
Wed 25 May 2016
SRR1295023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5409112
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC603651.1159872452262034No Hit
CCCATGTACTCTGCGTTGATACCAC562051.0390799820746917No Hit
GAGTACATGGGAAGCAGTGGTATCA283340.523819806282436No Hit
CATGTACTCTGCGTTGATACCACTG213330.39439005884884615No Hit
GCGTTGATACCACTGCTTCCCATGT182200.33683902274532307No Hit
GTATCAACGCAGAGTACATGGGAAG154960.28647955523938123No Hit
ACGCAGAGTACATGGGAAGCAGTGG150520.27827118388378724No Hit
TATCAACGCAGAGTACATGGGAAGC144420.2669939169312819No Hit
GCTTCCCATGTACTCTGCGTTGATA142160.26281578196199307No Hit
GGTATCAACGCAGAGTACATGGGAA135070.2497082700450647No Hit
ACTCTGCGTTGATACCACTGCTTCC127930.23650832151377157No Hit
ATTCCATTCCATTCCATTCCATTCC97150.17960434171080206No Hit
GTATCAACGCAGAGTACTTTTTTTT79270.14654900841395038No Hit
GTGGTATCAACGCAGAGTACATGGG78940.14593892675914272No Hit
GAATGGAATGGAATGGAATGGAATG71360.1319255360214394No Hit
CAGTGGTATCAACGCAGAGTACATG68040.12578774482761682No Hit
ATACCACTGCTTCCCATGTACTCTG62890.11626677354804264No Hit
GGTATCAACGCAGAGTACTTTTTTT62570.11567517921610793No Hit
ACATGGGAAGCAGTGGTATCAACGC60400.11166343015267571No Hit
GTACTCTGCGTTGATACCACTGCTT58580.10829873738979706No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN57390.1060987459679149No Hit
GGGAAGCAGTGGTATCAACGCAGAG56250.1039911911603975No Hit
GCAGTGGTATCAACGCAGAGTACAT55670.10291892643376584No Hit
CATGGGAAGCAGTGGTATCAACGCA54930.10155086454116684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCTA752.0767463E-411.3993399
CCGCTAA600.00588139111.0826899
GCACCGT1451.4495527E-810.48127656
TCGGAAC1201.5304431E-610.28925813
CTCGGAA1856.002665E-1110.26805812
GCCGTGC750.002650661610.1327468
TAACCGT856.6071586E-410.0574016
GTCTAGC3200.09.8063911
GTTACGT800.00452066739.4994498
GACTGCG1304.2480933E-69.4992727
ACTCGGA2201.6370905E-119.49795311
TAGACAG8000.09.3850535
GTCTTAC5300.09.3298071
TAACCCG2650.09.32453255
TCGCGTT1151.1126826E-49.08617710
CTAGACA6600.09.0723624
TTAGACA6500.09.0657154
CGCAAAA1701.6781814E-78.94446852
CTTAGAC3650.08.8525743
AGCACCG1951.6405465E-88.7727865
Produced by FastQC (version 0.11.5)