##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295023_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5409112
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206287094813344	33.0	33.0	33.0	27.0	33.0
2	31.05530020454374	33.0	33.0	33.0	27.0	33.0
3	31.177570551321548	33.0	33.0	33.0	27.0	33.0
4	34.74106803482716	37.0	37.0	37.0	27.0	37.0
5	34.99944759879256	37.0	37.0	37.0	33.0	37.0
6	34.98821044933069	37.0	37.0	37.0	33.0	37.0
7	34.83458338448159	37.0	37.0	37.0	33.0	37.0
8	34.789092553454246	37.0	37.0	37.0	33.0	37.0
9	34.68124934370004	37.0	37.0	37.0	27.0	37.0
10	34.599069496065155	37.0	37.0	37.0	27.0	37.0
11	34.80250233310014	37.0	37.0	37.0	33.0	37.0
12	34.71501459019521	37.0	37.0	37.0	27.0	37.0
13	34.624673329004835	37.0	37.0	37.0	27.0	37.0
14	36.00484885504312	40.0	37.0	40.0	27.0	40.0
15	36.16249561850448	40.0	37.0	40.0	27.0	40.0
16	36.23209354881171	40.0	37.0	40.0	27.0	40.0
17	36.16507903700275	40.0	37.0	40.0	27.0	40.0
18	36.096621219897095	40.0	37.0	40.0	27.0	40.0
19	36.17874227784524	40.0	37.0	40.0	27.0	40.0
20	36.200814107750034	40.0	37.0	40.0	27.0	40.0
21	36.19836453746937	40.0	37.0	40.0	27.0	40.0
22	36.09574769389135	40.0	37.0	40.0	27.0	40.0
23	36.007264038903244	37.0	37.0	40.0	27.0	40.0
24	36.07713040513859	40.0	37.0	40.0	27.0	40.0
25	34.92372370917814	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4599730881543529
1101	2	0.4915463458123135
1101	3	0.462737281558951
1101	4	0.5615358375565336
1101	5	0.4161516123744917
1101	6	0.3647612049821518
1101	7	0.38351759516816486
1101	8	0.5196007877697753
1101	9	0.5081214576303381
1101	10	0.6704217807698143
1101	11	0.5549578578641459
1101	12	0.6113844768561592
1101	13	0.5480975187758261
1101	14	1.0154562706821366
1101	15	0.8875174475300369
1101	16	0.9300693096536321
1101	17	0.8801910299658857
1101	18	0.9354172754063157
1101	19	0.8083075909269937
1101	20	0.783579964079351
1101	21	0.8314085523747181
1101	22	0.7956319683261697
1101	23	0.9151238215234798
1101	24	0.8595244619890181
1101	25	1.1013378252684802
1102	1	0.39619102845707843
1102	2	0.42351355864624907
1102	3	0.42233402828183486
1102	4	0.5870980859277353
1102	5	0.3892318956709815
1102	6	0.3282775110935816
1102	7	0.32797253558204886
1102	8	0.43528313027580623
1102	9	0.42918276645654885
1102	10	0.7087102986495424
1102	11	0.5388513255807723
1102	12	0.5447734730873606
1102	13	0.5407693232351036
1102	14	0.9262784498677732
1102	15	0.8339357104171938
1102	16	0.8015398880964781
1102	17	0.7017187647242054
1102	18	0.8440610270357993
1102	19	0.7983900677565217
1102	20	0.8400590247916213
1102	21	0.8859412025169959
1102	22	0.860409728008527
1102	23	0.9316563749284157
1102	24	0.8974296901123751
1102	25	1.005178767665761
1103	1	0.45485518427955185
1103	2	0.4271245885857695
1103	3	0.43807535646782725
1103	4	0.5436243542563872
1103	5	0.3908102982184758
1103	6	0.32430666322414226
1103	7	0.32482397516313455
1103	8	0.40928815793147777
1103	9	0.4817787485725731
1103	10	0.6092928164879439
1103	11	0.46302496300334894
1103	12	0.5521281233249411
1103	13	0.5474677463878379
1103	14	0.8596189234692915
1103	15	0.7663937580278528
1103	16	0.7294080615910516
1103	17	0.7054377891729828
1103	18	0.7745454650101209
1103	19	0.7181837402086799
1103	20	0.8427217941574341
1103	21	0.775229757750509
1103	22	0.7997811591250752
1103	23	0.9008366857023447
1103	24	0.8444056757353806
1103	25	0.97202905928701
1104	1	0.37215108297680644
1104	2	0.3197946194583601
1104	3	0.3904457020436034
1104	4	0.3443671792927745
1104	5	0.2750903063475576
1104	6	0.2509266075757708
1104	7	0.23854008778682356
1104	8	0.4066864173509046
1104	9	0.37327060006136037
1104	10	0.4370044790352807
1104	11	0.42081924634380385
1104	12	0.44467500752433153
1104	13	0.43167705837435477
1104	14	0.732803627041605
1104	15	0.6869431885436015
1104	16	0.557274131925567
1104	17	0.661419211822647
1104	18	0.7681969932482318
1104	19	0.6781844574808957
1104	20	0.6540922622672127
1104	21	0.6657975534235661
1104	22	0.6525669398011686
1104	23	0.5819817360757753
1104	24	0.6272686827198157
1104	25	0.718115681861363
1105	1	0.3199170467560286
1105	2	0.23046122163993843
1105	3	0.22545619327115318
1105	4	0.33320555486383796
1105	5	0.16520139431018066
1105	6	0.2109343457861641
1105	7	0.13059688922732704
1105	8	0.26453333471574325
1105	9	0.2578740167788709
1105	10	0.40555386221062406
1105	11	0.2720956790288156
1105	12	0.30445387328126117
1105	13	0.29334642845426373
1105	14	0.6239370516461378
1105	15	0.5159132625030338
1105	16	0.502981880552781
1105	17	0.4400965730929016
1105	18	0.5105353464975408
1105	19	0.5115480492010107
1105	20	0.4907500994374061
1105	21	0.5293115194052049
1105	22	0.38800310700603546
1105	23	0.5281805634392782
1105	24	0.5576805192054834
1105	25	0.4688609310466205
1106	1	0.04223531564822025
1106	2	-0.1636862335010818
1106	3	-0.1144263963107619
1106	4	-0.01729630622623546
1106	5	-0.32080902089661123
1106	6	-0.35730891224109484
1106	7	-0.6000564733220983
1106	8	-0.08704012642699865
1106	9	0.09069960952676581
1106	10	-0.2370849919620852
1106	11	-0.2879916926854662
1106	12	-0.6013035124128479
1106	13	-0.1594185028678865
1106	14	-0.33763893183672167
1106	15	-0.16394550597192392
1106	16	-0.1118786477038114
1106	17	-0.09025597945266384
1106	18	-0.35060048416128353
1106	19	-0.5354982710008329
1106	20	-0.14438068930672188
1106	21	0.050769394576455795
1106	22	0.19211296781043785
1106	23	-0.46130652467644495
1106	24	0.1237031581230994
1106	25	0.1202424552021526
1107	1	-1.0639996901930608
1107	2	-1.054727580882453
1107	3	-0.8527022512115536
1107	4	-0.8950794773206212
1107	5	-1.0096533928154017
1107	6	-0.8410990311579738
1107	7	-1.0771005633885693
1107	8	-0.801573928823899
1107	9	-0.9107860260173695
1107	10	-0.8077740653927918
1107	11	-0.7864980430780548
1107	12	-0.8072446393231587
1107	13	-0.7824982895737094
1107	14	-1.0504839811920803
1107	15	-0.9611742444690066
1107	16	-0.9724675633102748
1107	17	-0.9394459656906946
1107	18	-1.124718634211625
1107	19	-1.1438604708534825
1107	20	-1.1019650183316045
1107	21	-1.1153754071120474
1107	22	-1.0059019589615872
1107	23	-0.838109103814773
1107	24	-0.7339980154714567
1107	25	-0.7723570719381669
1108	1	0.03796196399601115
1108	2	-0.11625095906873995
1108	3	0.03897528488516144
1108	4	-0.10173964308594208
1108	5	-0.3569921257872508
1108	6	0.011209645090104914
1108	7	-0.011346544733122244
1108	8	0.14580881469898088
1108	9	-0.15306304198540488
1108	10	-0.5174952075452879
1108	11	-0.08335163219063446
1108	12	0.09080007034939541
1108	13	0.12867512138577553
1108	14	-0.1791256531101908
1108	15	0.12323693314346684
1108	16	-0.7412868775673687
1108	17	-0.6178423606958319
1108	18	-0.5654383051214182
1108	19	0.026792705776855996
1108	20	-0.8507006832542814
1108	21	-0.07904302620473658
1108	22	-0.18067994435787682
1108	23	-0.026088023748933153
1108	24	-0.26915525800720275
1108	25	-1.099214831638264
1109	1	0.17827624839272715
1109	2	0.17568543511688972
1109	3	0.2255988118629162
1109	4	0.2405735003584013
1109	5	0.17976866193770746
1109	6	0.21676689235807345
1109	7	0.2537447961350239
1109	8	0.3180401868671723
1109	9	0.32290025102323483
1109	10	0.37339162674502546
1109	11	0.26064908217816196
1109	12	0.33519374623320175
1109	13	0.2942962351017755
1109	14	0.5840586105913275
1109	15	0.4390203067805132
1109	16	0.406368130312849
1109	17	0.4207895777184518
1109	18	0.39596777665241945
1109	19	0.3799828902791873
1109	20	0.4328808979476051
1109	21	0.3688724633052516
1109	22	0.4917419955017337
1109	23	0.4876191809020156
1109	24	0.5068511040998445
1109	25	0.5157226508235766
1110	1	0.10168632976934688
1110	2	0.03690480837973098
1110	3	0.1395806628682088
1110	4	-0.8781834942806412
1110	5	-0.19920307075675225
1110	6	0.034982540027606035
1110	7	0.16469958543464003
1110	8	0.2809726867415492
1110	9	0.17991137565950055
1110	10	0.05130771567236536
1110	11	0.0935890283278269
1110	12	0.21019147833707308
1110	13	0.21179902342210255
1110	14	0.3841024441742391
1110	15	0.25073411666718926
1110	16	0.2524226748442686
1110	17	0.2884167420995709
1110	18	0.3919077037706913
1110	19	0.23771142115965205
1110	20	0.24916213634728734
1110	21	0.27905046634163
1110	22	-0.25975242885685645
1110	23	0.17233160429267969
1110	24	0.29682815658306794
1110	25	0.3903420279710659
1111	1	-0.05611713158591769
1111	2	-0.28880158836370384
1111	3	-0.13672768575067096
1111	4	-0.043130518951194574
1111	5	-0.17838757726449472
1111	6	-0.11363003236262159
1111	7	-0.049832696249865194
1111	8	0.08417270446497582
1111	9	0.013486797727800592
1111	10	-0.04462694983906346
1111	11	-0.103912354952989
1111	12	0.012595615076321565
1111	13	-0.16924319763671747
1111	14	-0.19330986487574364
1111	15	-0.172701922526592
1111	16	-0.161638016324261
1111	17	-0.09903488976709696
1111	18	-0.1418656982160158
1111	19	-0.12741452918844942
1111	20	-0.12515158995167752
1111	21	-0.2831236902500933
1111	22	-0.1372630650534461
1111	23	0.011863467665911287
1111	24	0.07238536082551406
1111	25	-0.09336145960875086
1112	1	-0.015715981012110802
1112	2	-0.3816118957361567
1112	3	-0.1449778022941075
1112	4	-0.08456862033216339
1112	5	-0.029303192926242616
1112	6	-0.055144409602569056
1112	7	0.07758854551986616
1112	8	0.13809398984552246
1112	9	-0.004757006600740965
1112	10	0.2339836613527666
1112	11	0.00557701332598981
1112	12	0.13181857218379633
1112	13	0.14236936735083106
1112	14	-0.22390508121590358
1112	15	-0.08948669854787994
1112	16	-0.08752281080771951
1112	17	-0.16271293956047117
1112	18	-0.06162156848974831
1112	19	-0.10195889015380999
1112	20	-0.8525609933823901
1112	21	-1.2029059462397669
1112	22	-1.6356387384568478
1112	23	-0.4420715351550868
1112	24	-0.16689855433242684
1112	25	-1.387981545230005
1113	1	-0.07967052660970708
1113	2	-0.12119407847953312
1113	3	-0.17223613068478372
1113	4	-0.4523213876265402
1113	5	-0.19080814194386164
1113	6	-0.08440743509963511
1113	7	-0.11899573717891343
1113	8	-0.0329633472806421
1113	9	-0.13391847698248682
1113	10	-0.01826060793206352
1113	11	-0.07705744575159201
1113	12	-0.13010476364446788
1113	13	-0.20458647770499283
1113	14	-0.46944249368695523
1113	15	-0.38453365617886703
1113	16	-0.3025840042188719
1113	17	-0.3118928777185701
1113	18	-0.7494621095343419
1113	19	-0.9946941492910426
1113	20	-1.134797936503567
1113	21	-0.9943866794886276
1113	22	-0.34408805189734437
1113	23	-2.0208502209698196
1113	24	-0.9208040996060731
1113	25	-0.3968825539009657
1114	1	-0.45879920179342193
1114	2	-0.560983824957102
1114	3	-0.4504392594798077
1114	4	-0.6713783317719617
1114	5	-0.7545051460240728
1114	6	-0.5210299377918162
1114	7	-0.4851599687324537
1114	8	-0.19177745378513578
1114	9	-1.2717204872826642
1114	10	-0.40108516924088633
1114	11	-0.4414633718032306
1114	12	-0.5080642212048545
1114	13	-0.40333315857273533
1114	14	-0.7925664655630626
1114	15	-1.4017816495069155
1114	16	-1.2605110730655156
1114	17	-1.7146172648522793
1114	18	-1.8107499003650886
1114	19	-1.7382762398800864
1114	20	-0.9708613267071797
1114	21	-0.7640071289815467
1114	22	-0.5961168130791421
1114	23	-0.4750620881057799
1114	24	-1.0678204438398993
1114	25	-0.6591186775143143
1115	1	-0.9000107294991082
1115	2	-0.6777776428331101
1115	3	-0.6849774108782718
1115	4	-0.8822721424051849
1115	5	-0.5660799298469001
1115	6	-0.413494468237694
1115	7	-0.42495024971248796
1115	8	-0.34177006010325783
1115	9	-0.5509844789454093
1115	10	-0.3243510239102676
1115	11	-0.5539853013630704
1115	12	-0.6877392412231274
1115	13	-0.5805378840911004
1115	14	-0.5585813313903714
1115	15	-1.6696129971085512
1115	16	-1.0094188703317641
1115	17	-0.7926946460202231
1115	18	-2.438810934957459
1115	19	-1.3404082860086177
1115	20	-1.075281189255115
1115	21	-1.1059403202757139
1115	22	-0.8959341338100231
1115	23	-0.5660001501764924
1115	24	-0.6125394436910199
1115	25	-0.808539606361947
1116	1	-1.8077773126374268
1116	2	-1.0949911267935981
1116	3	-1.9313136400911013
1116	4	-1.2338634092057745
1116	5	-1.2120516717236
1116	6	-0.6581783376021164
1116	7	-1.4306308676841937
1116	8	-3.0827368316980994
1116	9	-1.731752335718454
1116	10	-1.0322923435201048
1116	11	-0.7532493372319422
1116	12	-0.9388492708377214
1116	13	-1.8455672915920829
1116	14	-1.9082737952005075
1116	15	-1.7270034438126345
1116	16	-1.2180469689918638
1116	17	-1.9508676532478404
1116	18	-2.7368639823615197
1116	19	-1.674008426833268
1116	20	-1.1572313000509808
1116	21	-0.9401293877265786
1116	22	-0.8714624851261092
1116	23	-0.668974294356083
1116	24	-0.7496120512251352
1116	25	-0.7570174575434265
1201	1	0.3814579678178056
1201	2	0.30419566916506113
1201	3	0.39457969401643567
1201	4	0.5199151879374142
1201	5	0.33377662928673857
1201	6	0.14039557948068193
1201	7	0.19377164073526387
1201	8	0.3243230335904883
1201	9	0.32333389120060474
1201	10	0.493426214138708
1201	11	0.3131764553589349
1201	12	0.4157216268512798
1201	13	0.452541522715201
1201	14	0.9186650040098101
1201	15	0.7772704500440639
1201	16	0.6727804610394941
1201	17	0.642135482276494
1201	18	0.6404461442207534
1201	19	0.6405740839088025
1201	20	0.5916439757590339
1201	21	0.6696984487616362
1201	22	0.6802838277295393
1201	23	0.7003315353965576
1201	24	0.6829015941122236
1201	25	0.8276649254550676
1202	1	0.4437339162231453
1202	2	0.43299152658888573
1202	3	0.4490002318588786
1202	4	0.5725737844562033
1202	5	0.4285027630693179
1202	6	0.11499687816324666
1202	7	0.109069727013825
1202	8	0.27916489791005716
1202	9	0.27851246406034846
1202	10	0.49194800498060687
1202	11	0.338806858187759
1202	12	0.2515384969012757
1202	13	0.29530344320094315
1202	14	0.704008885599194
1202	15	0.5253921209878456
1202	16	0.3906216056497698
1202	17	0.47314709860892634
1202	18	0.5740024859175676
1202	19	0.5028681132069082
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2211	1	0.1880702432057504
2211	2	0.008003583913151147
2211	3	0.06355256257279152
2211	4	0.14387127806819677
2211	5	0.13285577093166978
2211	6	0.06912888129975414
2211	7	0.10004280343638783
2211	8	0.20550125840387778
2211	9	0.05308387806966408
2211	10	0.20602695764181078
2211	11	0.1545413729841556
2211	12	0.23783786778363947
2211	13	0.05382753797043449
2211	14	0.030358136819067738
2211	15	0.037241853770915156
2211	16	-0.07031231344095801
2211	17	0.003668534315870886
2211	18	0.027434081262263987
2211	19	-0.015945691289473984
2211	20	-0.04172664944887572
2211	21	0.014908923167972432
2211	22	0.07819834238075174
2211	23	0.22086348443683335
2211	24	0.23058587958472998
2211	25	0.1078555867292934
2212	1	0.149489568260055
2212	2	0.074042913010814
2212	3	0.13924434123320495
2212	4	0.17120188213177556
2212	5	0.22209913205079346
2212	6	0.1728203295878572
2212	7	0.29982220595559994
2212	8	0.30911381181201847
2212	9	0.19630535941965377
2212	10	0.4140886275505622
2212	11	0.17997156580817375
2212	12	0.2821541388664457
2212	13	0.13744224249678183
2212	14	0.17367684994496102
2212	15	0.2167566749137606
2212	16	0.08396308023216648
2212	17	0.20505742320476372
2212	18	0.2940852154994431
2212	19	0.30149648739264023
2212	20	0.3176859103694696
2212	21	0.24379144499349792
2212	22	-0.37374604659073185
2212	23	0.2158915660831653
2212	24	0.19053517113336227
2212	25	0.4001504846960202
2213	1	-0.09275099057015268
2213	2	-0.8015279251245104
2213	3	-0.2889287940812615
2213	4	-0.12323324638094135
2213	5	-0.04397713498793365
2213	6	-0.09827460924908848
2213	7	-0.023474176582347184
2213	8	0.0824332812320634
2213	9	-0.26742353832559473
2213	10	-1.6311903849318696
2213	11	-0.4536911213909889
2213	12	-0.11014467236043401
2213	13	-0.1471750200098043
2213	14	-0.07685814509396494
2213	15	-0.13154394987559925
2213	16	-0.11085499985054525
2213	17	-0.1945220538041994
2213	18	-0.10365001144922559
2213	19	-0.15251672172129105
2213	20	-0.15362070895681512
2213	21	-0.19425818188477706
2213	22	-0.037513841460587116
2213	23	-1.0096900544411582
2213	24	-1.553716332705477
2213	25	-1.4425500245312648
2214	1	0.008536711498734917
2214	2	-0.25549920062989173
2214	3	-0.08701044650277723
2214	4	-0.07014671089373792
2214	5	-0.05266197131201977
2214	6	-0.15760616055808185
2214	7	-0.058821017021550404
2214	8	-0.04464316726485151
2214	9	-0.4489801386931518
2214	10	-0.038549353278789056
2214	11	-0.20442126596610422
2214	12	-0.18883430999196094
2214	13	-0.07640036470041167
2214	14	-0.0975913496946319
2214	15	-0.2176631694166602
2214	16	-0.23583684541929273
2214	17	-0.28391370152618833
2214	18	-0.047971293295091755
2214	19	-0.8617413867813752
2214	20	-0.3597653309546658
2214	21	-0.25865562141900256
2214	22	-0.6114962901109848
2214	23	-1.8858770503647762
2214	24	-1.3973635816995724
2214	25	-1.714429050491951
2215	1	-0.49082438396068895
2215	2	-0.33244755328867726
2215	3	-1.277414134720022
2215	4	-0.971120969407842
2215	5	-0.42307698713409536
2215	6	-0.3971152435649259
2215	7	-0.30362882430567595
2215	8	-0.16658854548980884
2215	9	-0.39916813340209245
2215	10	-1.2661610688856513
2215	11	-0.6469967133707684
2215	12	-0.6827883753723469
2215	13	-0.5938891644967228
2215	14	-0.9671065823727858
2215	15	-1.1362355304264113
2215	16	-0.767485139382174
2215	17	-0.6057618134033476
2215	18	-0.5693867539945714
2215	19	-1.1568135060315967
2215	20	-1.5782438269362302
2215	21	-0.7749069390149828
2215	22	-0.5673458086106393
2215	23	-0.8946518971766224
2215	24	-1.9452090878288715
2215	25	-0.9818664922609628
2216	1	-0.7770924281748854
2216	2	-0.6530069978626258
2216	3	-1.6299178691054763
2216	4	-1.0166323424671546
2216	5	-0.7804632565410117
2216	6	-0.679340989485226
2216	7	-0.4960779722975204
2216	8	-0.6736179172432415
2216	9	-0.4665734284591352
2216	10	-0.5442447057827735
2216	11	-0.7013787015715067
2216	12	-0.4951408700462139
2216	13	-1.3990996469862864
2216	14	-1.0161627222475573
2216	15	-1.4701862840880224
2216	16	-1.1932205204808497
2216	17	-2.137634198898624
2216	18	-1.1877552479586697
2216	19	-0.9392566320369511
2216	20	-0.9026795085253596
2216	21	-0.677554366058196
2216	22	-0.7870704672680873
2216	23	-0.6153250114569389
2216	24	-0.7501956666562961
2216	25	-0.7525901570152058
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6397.0
3	683.0
4	897.0
5	719.0
6	519.0
7	376.0
8	612.0
9	1213.0
10	2406.0
11	4502.0
12	7918.0
13	12004.0
14	16454.0
15	21669.0
16	26021.0
17	27963.0
18	28901.0
19	29342.0
20	30638.0
21	32347.0
22	35078.0
23	37387.0
24	39046.0
25	41814.0
26	43524.0
27	49650.0
28	55454.0
29	65393.0
30	79943.0
31	101486.0
32	135588.0
33	192015.0
34	279479.0
35	446459.0
36	813693.0
37	2741522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8815736785905	21.20487779557454	13.152640594411816	22.760907931423148
2	14.149391570070133	21.863293860184353	40.337609030461955	23.64970553928356
3	18.122976686951578	27.790391667288667	30.111435826932293	23.975195818827462
4	11.721957784428087	18.257303994168474	38.264695285226956	31.75604293617648
5	12.310523871870538	39.51105588552145	35.12833587864338	13.050084363964636
6	30.7210889443721	38.25782753775351	17.80808014770445	13.213003370169933
7	24.916424163551767	33.71594901610068	24.138086857383723	17.229539962963834
8	23.914898251856155	37.95886136326842	21.652910442591182	16.473329942284245
9	24.21445177921015	17.286978898362523	22.16949143751467	36.32907788491265
10	14.710587761048261	31.068198036177545	33.66352701089505	20.557687191879133
11	30.950789005778635	27.232978959845905	22.480089642910063	19.3361423914654
12	20.37392082123441	29.24341768327864	31.936615942108176	18.446045553378774
13	26.124339445192312	24.6941863026214	26.469985104578235	22.711489147608056
14	19.777839560013344	24.508588893533876	28.344839708747593	27.36873183770519
15	24.28982329158093	29.94797464111022	23.713340087307774	22.048861980001075
16	20.674071814487174	31.86245338907475	27.237375345351	20.226099451087073
17	20.417704788808358	31.159129585054895	29.045342508073464	19.37782311806329
18	20.620259394065567	30.236821228949907	29.797644623499274	19.34527475348525
19	20.66829262510298	29.754650428814433	30.102210157597455	19.47484678848513
20	22.12488019002903	28.861594595470603	28.63017035009978	20.38335486440058
21	21.497627845918146	30.086629555765626	28.470368076525304	19.945374521790924
22	20.486219346201707	29.613046701809388	28.448914055920522	21.451819896068383
23	19.489853884903454	30.503691171804658	29.041484448031962	20.964970495259923
24	20.43592944554059	30.14147459538925	28.440485768160684	20.982110190909474
25	21.35732687624062	28.440746961938373	27.038452105655857	23.16347405616515
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12171.0
1	12171.0
2	11477.5
3	10784.0
4	10784.0
5	10784.0
6	14435.5
7	18087.0
8	18087.0
9	18087.0
10	23547.5
11	29008.0
12	29008.0
13	29008.0
14	43827.5
15	58647.0
16	58647.0
17	58647.0
18	89211.5
19	119776.0
20	119776.0
21	119776.0
22	166297.5
23	212819.0
24	212819.0
25	212819.0
26	271903.0
27	330987.0
28	330987.0
29	330987.0
30	389896.0
31	448805.0
32	448805.0
33	448805.0
34	501225.0
35	553645.0
36	553645.0
37	553645.0
38	592016.0
39	630387.0
40	630387.0
41	630387.0
42	624210.5
43	618034.0
44	618034.0
45	618034.0
46	700265.5
47	782497.0
48	782497.0
49	782497.0
50	691676.0
51	600855.0
52	600855.0
53	600855.0
54	497574.0
55	394293.0
56	394293.0
57	394293.0
58	329220.5
59	264148.0
60	264148.0
61	264148.0
62	216462.5
63	168777.0
64	168777.0
65	168777.0
66	128858.5
67	88940.0
68	88940.0
69	88940.0
70	64731.0
71	40522.0
72	40522.0
73	40522.0
74	28614.0
75	16706.0
76	16706.0
77	16706.0
78	11412.0
79	6118.0
80	6118.0
81	6118.0
82	4120.0
83	2122.0
84	2122.0
85	2122.0
86	1388.5
87	655.0
88	655.0
89	655.0
90	446.5
91	238.0
92	238.0
93	238.0
94	147.0
95	56.0
96	56.0
97	56.0
98	45.5
99	35.0
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27494346576665446
2	0.164555660892213
3	0.18230349085025416
4	0.20090173766045147
5	0.2266176037767382
6	0.17897577273312146
7	0.17609175036493974
8	0.19570679993314985
9	0.1963538562337034
10	0.20461768955791637
11	0.1832463443167751
12	0.18884800314728184
13	0.1998294729338198
14	0.1889589270845196
15	0.18158248525820875
16	0.18293205982793478
17	0.18376398935721797
18	0.18755389054617466
19	0.18561272164451392
20	0.1837085273885991
21	0.18117576415500364
22	0.18459591888650115
23	0.1876278398376665
24	0.20149333199238617
25	0.1960395717448631
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5409112.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.23319368005134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.75967230916912	54.814374049507805
2	11.979435845847675	15.868725891115576
3	2.9502203623574057	5.86207549976548
4	0.9941049681229214	2.6337098756794695
5	0.45410855346766876	1.5038529886796028
6	0.22507032483071354	0.8944275849686832
7	0.13078299225146986	0.6063522679093782
8	0.08162706611197754	0.43251370234631714
9	0.06224060692279214	0.3710154755773113
>10	0.316414653493347	3.7899686456314203
>50	0.02532028341813047	1.1574257729081316
>100	0.017254903930734648	2.2897735455625274
>500	0.001662263654117611	0.7615910242648882
>1k	0.0012678241754677318	1.8872623623861735
>5k	5.071296701783641E-4	2.0892991006312975
>10k+	3.0991257622011136E-4	5.037632213066048
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	60365	1.1159872452262034	No Hit
CCCATGTACTCTGCGTTGATACCAC	56205	1.0390799820746917	No Hit
GAGTACATGGGAAGCAGTGGTATCA	28334	0.523819806282436	No Hit
CATGTACTCTGCGTTGATACCACTG	21333	0.39439005884884615	No Hit
GCGTTGATACCACTGCTTCCCATGT	18220	0.33683902274532307	No Hit
GTATCAACGCAGAGTACATGGGAAG	15496	0.28647955523938123	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	15052	0.27827118388378724	No Hit
TATCAACGCAGAGTACATGGGAAGC	14442	0.2669939169312819	No Hit
GCTTCCCATGTACTCTGCGTTGATA	14216	0.26281578196199307	No Hit
GGTATCAACGCAGAGTACATGGGAA	13507	0.2497082700450647	No Hit
ACTCTGCGTTGATACCACTGCTTCC	12793	0.23650832151377157	No Hit
ATTCCATTCCATTCCATTCCATTCC	9715	0.17960434171080206	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7927	0.14654900841395038	No Hit
GTGGTATCAACGCAGAGTACATGGG	7894	0.14593892675914272	No Hit
GAATGGAATGGAATGGAATGGAATG	7136	0.1319255360214394	No Hit
CAGTGGTATCAACGCAGAGTACATG	6804	0.12578774482761682	No Hit
ATACCACTGCTTCCCATGTACTCTG	6289	0.11626677354804264	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6257	0.11567517921610793	No Hit
ACATGGGAAGCAGTGGTATCAACGC	6040	0.11166343015267571	No Hit
GTACTCTGCGTTGATACCACTGCTT	5858	0.10829873738979706	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5739	0.1060987459679149	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	5625	0.1039911911603975	No Hit
GCAGTGGTATCAACGCAGAGTACAT	5567	0.10291892643376584	No Hit
CATGGGAAGCAGTGGTATCAACGCA	5493	0.10155086454116684	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.8487322872959554E-5	0.0
3	0.0	0.0	0.0	3.697464574591911E-5	0.0
4	0.0	0.0	0.0	3.697464574591911E-5	0.0
5	0.0	0.0	0.0	3.697464574591911E-5	0.0
6	0.0	0.0	0.0	3.697464574591911E-5	0.0
7	0.0	0.0	0.0	5.5461968618878664E-5	0.0
8	0.0	0.0	0.0	5.5461968618878664E-5	0.0
9	0.0	0.0	0.0	7.394929149183821E-5	0.0
10	1.8487322872959554E-5	0.0	0.0	1.2941126011071689E-4	0.0
11	1.8487322872959554E-5	0.0	0.0	1.66385905856636E-4	0.0
12	1.8487322872959554E-5	0.0	0.0	1.8487322872959554E-4	0.0
13	1.8487322872959554E-5	0.0	0.0	1.8487322872959554E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCCTA	75	2.0767463E-4	11.399339	9
CCGCTAA	60	0.005881391	11.082689	9
GCACCGT	145	1.4495527E-8	10.4812765	6
TCGGAAC	120	1.5304431E-6	10.289258	13
CTCGGAA	185	6.002665E-11	10.268058	12
GCCGTGC	75	0.0026506616	10.132746	8
TAACCGT	85	6.6071586E-4	10.057401	6
GTCTAGC	320	0.0	9.806391	1
GTTACGT	80	0.0045206673	9.499449	8
GACTGCG	130	4.2480933E-6	9.499272	7
ACTCGGA	220	1.6370905E-11	9.497953	11
TAGACAG	800	0.0	9.385053	5
GTCTTAC	530	0.0	9.329807	1
TAACCCG	265	0.0	9.3245325	5
TCGCGTT	115	1.1126826E-4	9.086177	10
CTAGACA	660	0.0	9.072362	4
TTAGACA	650	0.0	9.065715	4
CGCAAAA	170	1.6781814E-7	8.9444685	2
CTTAGAC	365	0.0	8.852574	3
AGCACCG	195	1.6405465E-8	8.772786	5
>>END_MODULE