FastQCFastQC Report
Wed 25 May 2016
SRR1295023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5409112
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC620601.1473232574958698No Hit
GTACATGGGAAGCAGTGGTATCAAC593761.0977032829048465No Hit
GAGTACATGGGAAGCAGTGGTATCA306910.5673944262940017No Hit
CATGTACTCTGCGTTGATACCACTG238660.44121844768605273No Hit
GTATCAACGCAGAGTACTTTTTTTT214930.39734803050851975No Hit
GCGTTGATACCACTGCTTCCCATGT197770.3656237844585211No Hit
ACGCAGAGTACATGGGAAGCAGTGG188970.34935494033031667No Hit
GGTATCAACGCAGAGTACTTTTTTT185310.3425885801588135No Hit
GTATCAACGCAGAGTACATGGGAAG164630.30435679645753316No Hit
GCTTCCCATGTACTCTGCGTTGATA157200.2906207155629242No Hit
TATCAACGCAGAGTACATGGGAAGC157010.29026945642833796No Hit
TATCAACGCAGAGTACTTTTTTTTT149420.2762375783677617No Hit
GGTATCAACGCAGAGTACATGGGAA146130.27015524914255795No Hit
ACTCTGCGTTGATACCACTGCTTCC144610.2673451760658681No Hit
ATTCCATTCCATTCCATTCCATTCC93450.17276403224780704No Hit
ACGCAGAGTACTTTTTTTTTTTTTT88220.16309516238524918No Hit
CAGTGGTATCAACGCAGAGTACATG83320.15403637417749902No Hit
GTGGTATCAACGCAGAGTACATGGG82760.15300108409661328No Hit
GAATGGAATGGAATGGAATGGAATG79340.14667841967406112No Hit
ACATGGGAAGCAGTGGTATCAACGC78350.1448481747096381No Hit
CATGGGAAGCAGTGGTATCAACGCA73550.13597425973061752No Hit
GCAGTGGTATCAACGCAGAGTACAT67360.12453060687225555No Hit
ATACCACTGCTTCCCATGTACTCTG65410.12092557891202846No Hit
GGGAAGCAGTGGTATCAACGCAGAG65190.12051885780882333No Hit
CCATGTACTCTGCGTTGATACCACT58950.10898276833609657No Hit
GTACTCTGCGTTGATACCACTGCTT56770.1049525319497914No Hit
CTGCTTCCCATGTACTCTGCGTTGA56720.1048600953354266No Hit
CTTCCCATGTACTCTGCGTTGATAC55330.10229035745608521No Hit
GTACTTTTTTTTTTTTTTTTTTTTT54530.10081137162624845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG907.476421E-611.6102195
TCGCTCA909.5324904E-510.55464659
TGCACCG1201.5273617E-610.2908755
TATTCCG2900.09.8268315
GACGGTC800.0045217189.49918212
GGACGCA1401.0613257E-69.4991828
GTCTTAG5650.09.429851
TCGGAAC1356.8629906E-69.14736113
AGTACTC9550.09.051665
CTAGACT4950.09.0195084
GTCTCGC2252.5465852E-108.8797741
GTCGCTC1201.7669692E-48.7075838
CTAGGAC4700.08.6908213
TATACTG11550.08.6356995
TAGACAG8150.08.6251055
ACAGCGT1554.217116E-68.5799068
TAGGACC3150.08.4437954
ACGCAGT1804.0452323E-78.44371810
GTCCTAC2951.8189894E-128.385261
TACGCTG1252.7478492E-48.3593575
Produced by FastQC (version 0.11.5)