FastQCFastQC Report
Wed 25 May 2016
SRR1295022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4491830
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC459211.0223227504157548No Hit
CCCATGTACTCTGCGTTGATACCAC415780.9256360993180952No Hit
GAGTACATGGGAAGCAGTGGTATCA216270.48147414305528036No Hit
CATGTACTCTGCGTTGATACCACTG159340.35473292622383307No Hit
GCGTTGATACCACTGCTTCCCATGT138120.3074916014185755No Hit
GCTTCCCATGTACTCTGCGTTGATA123780.2755669738169076No Hit
ACGCAGAGTACATGGGAAGCAGTGG118560.2639458750665096No Hit
GTATCAACGCAGAGTACATGGGAAG116590.25956013473350503No Hit
TATCAACGCAGAGTACATGGGAAGC103420.23024023616209874No Hit
GGTATCAACGCAGAGTACATGGGAA95570.21276406275393325No Hit
ACTCTGCGTTGATACCACTGCTTCC92060.20494987566314843No Hit
GTATCAACGCAGAGTACTTTTTTTT61710.13738275936533662No Hit
GTGGTATCAACGCAGAGTACATGGG61640.1372269208763466No Hit
GGTATCAACGCAGAGTACTTTTTTT51410.11445223884252076No Hit
CAGTGGTATCAACGCAGAGTACATG50490.11240407584436633No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN47570.10590338458935444No Hit
GCAGTGGTATCAACGCAGAGTACAT46960.10454536347101293No Hit
ACATGGGAAGCAGTGGTATCAACGC45770.10189610915818274No Hit
ATACCACTGCTTCCCATGTACTCTG45140.10049356275727264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAC500.001493212913.3083793
GTCTACG655.3970303E-513.1693371
TAGGCGT1303.2787284E-810.9599996
AATACCG909.4530915E-510.5632675
GACCGGG856.605273E-410.0576357
AGTACCG1356.387327E-79.859055
CTAGTAC2650.09.6853443
CGAATAC900.00110797539.5059843
GACCGTG1106.8512585E-59.49887857
GTAGGAC1856.6374923E-99.2490663
GCACCGT1356.866745E-69.1468656
GTCTTGC7500.09.1307411
ATACCGT1151.1132316E-49.0856816
TATACCG950.00181276729.0065765
CCATGCG950.00182662888.9990399
GCCCTAG2750.08.9923971
CTTGGAC5200.08.9575623
GTCTAGG4900.08.9288711
CTAGCAC4300.08.8427763
GTATAGA7550.08.6923451
Produced by FastQC (version 0.11.5)