##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295022_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4491830
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15127531540597	33.0	33.0	33.0	27.0	33.0
2	31.191318905657607	33.0	33.0	33.0	27.0	33.0
3	31.21697437347362	33.0	33.0	33.0	27.0	33.0
4	34.8096813548153	37.0	37.0	37.0	33.0	37.0
5	35.02515722990407	37.0	37.0	37.0	33.0	37.0
6	34.997896180398634	37.0	37.0	37.0	33.0	37.0
7	34.90773871673683	37.0	37.0	37.0	33.0	37.0
8	34.80878417037154	37.0	37.0	37.0	33.0	37.0
9	34.785147256240776	37.0	37.0	37.0	33.0	37.0
10	34.698418461963165	37.0	37.0	37.0	27.0	37.0
11	34.855935999358834	37.0	37.0	37.0	33.0	37.0
12	34.77327214075332	37.0	37.0	37.0	27.0	37.0
13	34.683772092888645	37.0	37.0	37.0	27.0	37.0
14	36.08211975965253	40.0	37.0	40.0	27.0	40.0
15	36.223892934505535	40.0	37.0	40.0	27.0	40.0
16	36.29867470496435	40.0	37.0	40.0	27.0	40.0
17	36.242979587384205	40.0	37.0	40.0	27.0	40.0
18	36.17525373845404	40.0	37.0	40.0	27.0	40.0
19	36.26025539702081	40.0	37.0	40.0	27.0	40.0
20	36.255945572294586	40.0	37.0	40.0	27.0	40.0
21	36.248826202238284	40.0	37.0	40.0	27.0	40.0
22	36.165484223579256	40.0	37.0	40.0	27.0	40.0
23	36.08084099353716	40.0	37.0	40.0	27.0	40.0
24	36.12554415460959	40.0	37.0	40.0	27.0	40.0
25	34.94251919596245	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5396865608277714
1101	2	0.5686575387895658
1101	3	0.5355189533164442
1101	4	0.6784737304259281
1101	5	0.5218055313706387
1101	6	0.41882094978129913
1101	7	0.4709872167150877
1101	8	0.6253357920043285
1101	9	0.6082377618810213
1101	10	0.746830214471288
1101	11	0.6308961107303048
1101	12	0.6310620783560381
1101	13	0.6663364543924502
1101	14	1.1881798412719462
1101	15	1.0289993197508096
1101	16	1.0123076363803207
1101	17	0.9599149191558709
1101	18	1.0118995085853797
1101	19	0.9502585893614679
1101	20	0.9180324919930385
1101	21	0.9804097240015324
1101	22	0.9599176557584599
1101	23	0.9871375765118131
1101	24	1.015917400809137
1101	25	1.1943170076530052
1102	1	0.44800622677761837
1102	2	0.45022882249631024
1102	3	0.5206362687053456
1102	4	0.514162744859533
1102	5	0.4065330318802012
1102	6	0.40699849948035194
1102	7	0.36052987301973616
1102	8	0.4716396644787224
1102	9	0.4554332450520633
1102	10	0.6331254153957317
1102	11	0.5712998980759636
1102	12	0.5597946072522078
1102	13	0.5201832134967574
1102	14	0.937872734101667
1102	15	0.9162386211585769
1102	16	0.8723970608190754
1102	17	0.8713137132853248
1102	18	0.8827935885717153
1102	19	0.7702381283641415
1102	20	0.8159148900062192
1102	21	0.7622062659817459
1102	22	0.8494484318270068
1102	23	0.9453725962906674
1102	24	0.905424682228336
1102	25	1.0177951841704953
1103	1	0.36959296484067394
1103	2	0.2809722606038143
1103	3	0.34167409743399446
1103	4	0.44161890868323184
1103	5	0.2954252647428106
1103	6	0.2299101908289316
1103	7	0.2922775789137759
1103	8	0.5169486229937945
1103	9	0.4628639723672947
1103	10	0.5428930126995439
1103	11	0.4011994152106695
1103	12	0.5170887392245476
1103	13	0.3729953811428004
1103	14	0.7782053611769086
1103	15	0.7228820993883431
1103	16	0.6019168226605842
1103	17	0.6415336769411866
1103	18	0.6736504468617568
1103	19	0.616126035077599
1103	20	0.6403234848848101
1103	21	0.6472867413623149
1103	22	0.7091404381646385
1103	23	0.756308652974262
1103	24	0.7366146187252554
1103	25	0.7802574720045072
1104	1	0.17093367987849817
1104	2	0.15082786160411032
1104	3	0.19290577407760168
1104	4	0.15754919993712946
1104	5	0.14749644284285068
1104	6	0.07041240013226258
1104	7	0.161588330046321
1104	8	0.18780351990518085
1104	9	0.18287837371020998
1104	10	0.362755725424428
1104	11	0.16160438747736094
1104	12	0.2512925202793781
1104	13	0.2282773801403195
1104	14	0.586014545918907
1104	15	0.5847814836586878
1104	16	0.32921072009253294
1104	17	0.40753467491698103
1104	18	0.48392486847167504
1104	19	0.3338175145392839
1104	20	0.43101629404178254
1104	21	0.3145385455951768
1104	22	0.4097430310503185
1104	23	0.4695736574050997
1104	24	0.4850228722861303
1104	25	0.7981883352551833
1105	1	0.21818313381040966
1105	2	0.2560377174264019
1105	3	0.17660939639070605
1105	4	0.25992375422119096
1105	5	0.23549759520260238
1105	6	0.24410457750717285
1105	7	0.20259284460247784
1105	8	0.35229845055465603
1105	9	0.27065989436296434
1105	10	0.4418745143454501
1105	11	0.23947236952532336
1105	12	0.3111011715317602
1105	13	0.3107504995300019
1105	14	0.6050873535225207
1105	15	0.4924409151382676
1105	16	0.3779974730681914
1105	17	0.4348960906283068
1105	18	0.36254448995295974
1105	19	0.435813274799294
1105	20	0.4147264303338858
1105	21	0.47478156863204646
1105	22	0.4396458818133411
1105	23	0.6673591995180956
1105	24	0.5198840901199802
1105	25	0.4429792168644724
1106	1	-0.020154573437032752
1106	2	-0.1429974207283351
1106	3	-0.1701843091741182
1106	4	-0.05173046289486649
1106	5	-0.4981220998010727
1106	6	-0.3955047057270775
1106	7	-0.762957997164257
1106	8	-0.3741180567665623
1106	9	-0.007760177708256322
1106	10	-0.36653344360415474
1106	11	-0.47511583102946986
1106	12	-0.8890028981489095
1106	13	-0.3265277188845559
1106	14	-0.4438060575449825
1106	15	-0.20787414805108995
1106	16	-0.12871764208264835
1106	17	-0.23992883610746674
1106	18	-0.4921515360203159
1106	19	-0.5920153285850631
1106	20	-0.13885425087639192
1106	21	0.004217830520602206
1106	22	0.22875867011389772
1106	23	-0.5322102510225264
1106	24	0.07436336094134788
1106	25	0.1977848207560342
1107	1	-1.1670169084276019
1107	2	-1.1519239063234288
1107	3	-0.8767364876659514
1107	4	-0.9933957009084651
1107	5	-1.081137423125675
1107	6	-1.143249180921579
1107	7	-1.1860740935942573
1107	8	-1.1036903262023685
1107	9	-1.171465401680237
1107	10	-1.012938054479882
1107	11	-1.0120938460997237
1107	12	-1.0867384748757445
1107	13	-1.0166674003357414
1107	14	-1.24046199706806
1107	15	-1.0893201082284065
1107	16	-1.155272563588369
1107	17	-1.242498125781161
1107	18	-1.3666092111527703
1107	19	-1.271217242163793
1107	20	-1.3684728576539271
1107	21	-1.2655213382482273
1107	22	-1.263156851589386
1107	23	-1.1799314432539205
1107	24	-1.0068659664487853
1107	25	-1.018749283233582
1108	1	0.005276587743765049
1108	2	-0.08044644194425032
1108	3	-0.04656066135985526
1108	4	-0.21778990464986236
1108	5	-0.4340841130549151
1108	6	0.005235456085308954
1108	7	0.015965258930698667
1108	8	0.21102073321412718
1108	9	-0.266259334024916
1108	10	-0.5999221583860574
1108	11	-0.09598092567711802
1108	12	0.0699840219741148
1108	13	0.16907409428478815
1108	14	-0.3054063607396671
1108	15	0.040367785539167755
1108	16	-0.7420591294505385
1108	17	-0.6643838483749391
1108	18	-0.6029219958770113
1108	19	-0.004083385422497088
1108	20	-0.9759078678313031
1108	21	-0.09028725857390185
1108	22	-0.26477776852259893
1108	23	-0.070420151211664
1108	24	-0.35374189937502365
1108	25	-1.0957311578095243
1109	1	0.1156033612548697
1109	2	0.112361725110528
1109	3	0.17722668472784164
1109	4	0.12424965214358963
1109	5	0.08233713102830365
1109	6	0.11007922273218895
1109	7	0.17046353146011484
1109	8	0.2085010613574454
1109	9	0.16727984987841182
1109	10	0.21413313589274452
1109	11	0.11624340197915473
1109	12	0.17869519193591543
1109	13	0.16326880381098618
1109	14	0.45745796813420014
1109	15	0.30463958439157324
1109	16	0.2961944510556833
1109	17	0.3455590005056308
1109	18	0.3358376498012703
1109	19	0.2496135538344646
1109	20	0.16382205317687237
1109	21	0.21202542121240953
1109	22	0.28255606707090664
1109	23	0.3442526947771043
1109	24	0.3961565070904314
1109	25	0.2848534425892453
1110	1	-0.022014030608481505
1110	2	-0.011809323366378521
1110	3	0.07445049833026829
1110	4	-1.055886519581776
1110	5	-0.3504202802341254
1110	6	-0.013793654665988697
1110	7	0.02114429554949737
1110	8	0.1886928210962182
1110	9	0.14518370908569267
1110	10	-0.15165953216173023
1110	11	-0.08929809901065511
1110	12	0.13550179291796383
1110	13	0.01813663101923879
1110	14	0.2156490698924074
1110	15	0.13698821577308706
1110	16	0.15246691551433145
1110	17	0.20018944420763773
1110	18	0.3042693304311115
1110	19	0.22781634041997734
1110	20	0.16540829684522862
1110	21	0.24338159857220631
1110	22	-0.37188851602844863
1110	23	-4.227676527719382E-4
1110	24	0.2451037381834169
1110	25	0.306993543290524
1111	1	0.013216934904487943
1111	2	-0.23862802655728288
1111	3	-0.10873720961540911
1111	4	0.030745215821006866
1111	5	0.0017127943128230072
1111	6	-0.10145014140703523
1111	7	0.04156027802922324
1111	8	0.09364107542835853
1111	9	0.02467462724651881
1111	10	-0.020247109823650078
1111	11	-0.0897469835310929
1111	12	0.10242927837363425
1111	13	-0.0857161099730348
1111	14	-0.21105467367128483
1111	15	0.002783862249714275
1111	16	-0.07467338456197581
1111	17	0.05378173695088151
1111	18	-0.0342758558682732
1111	19	-0.06551442680481045
1111	20	-0.03971047902557956
1111	21	-0.2012919339297028
1111	22	-0.10182247435615466
1111	23	0.0035568506770715658
1111	24	0.0014622278726932336
1111	25	-0.02387711462911568
1112	1	-0.09881594019930162
1112	2	-0.4101105423883169
1112	3	-0.19617991287913483
1112	4	-0.09710939545205832
1112	5	-0.10033518959338039
1112	6	-0.1931882804046765
1112	7	-0.1448259782613306
1112	8	-0.05252288331367794
1112	9	-0.0892844090599354
1112	10	0.17231814846554272
1112	11	-0.0683758394875511
1112	12	-0.09095345235909491
1112	13	-0.06315785839367294
1112	14	-0.25243963193818786
1112	15	-0.2698866882065971
1112	16	-0.15196721399038893
1112	17	-0.21520332783486396
1112	18	-0.14621747860037715
1112	19	-0.1724724614806732
1112	20	-1.0193436818899357
1112	21	-1.4291789460934083
1112	22	-1.8170284085789845
1112	23	-0.591274607904893
1112	24	-0.3634718319549677
1112	25	-1.5057468961956175
1113	1	-0.2417666313997202
1113	2	-0.2580593339131738
1113	3	-0.274068571584948
1113	4	-0.5744224517618619
1113	5	-0.31821278214636806
1113	6	-0.2334745663435882
1113	7	-0.1553655715472999
1113	8	-0.1828768979730384
1113	9	-0.22632432941500724
1113	10	-0.08360701173949536
1113	11	-0.24515447988001426
1113	12	-0.17975897865745338
1113	13	-0.19439302002294312
1113	14	-0.5357044355083715
1113	15	-0.4657927438233287
1113	16	-0.4745836603212936
1113	17	-0.4764099082045803
1113	18	-0.906923617346294
1113	19	-1.0375642669959078
1113	20	-1.2744354495937174
1113	21	-1.2261978068678445
1113	22	-0.6106016654043387
1113	23	-2.1522525129905787
1113	24	-1.135816056808217
1113	25	-0.7072629115179367
1114	1	-0.2708397598066483
1114	2	-0.378678919297311
1114	3	-0.34468241124024956
1114	4	-0.44664536695803747
1114	5	-0.6125212889209735
1114	6	-0.38610996758239224
1114	7	-0.4618342242500404
1114	8	-0.11383345772802755
1114	9	-1.2271903306150946
1114	10	-0.26586841683158724
1114	11	-0.3450593804328008
1114	12	-0.4736106815260044
1114	13	-0.4555863674809686
1114	14	-0.8907121909947477
1114	15	-1.33971933698259
1114	16	-1.3222546380710867
1114	17	-1.6166214408494497
1114	18	-1.913923545866517
1114	19	-1.5518246230258939
1114	20	-0.8680163257322278
1114	21	-0.7004242757728534
1114	22	-0.4648387027776053
1114	23	-0.45171343248324547
1114	24	-0.9083841608612175
1114	25	-0.5122456091592866
1115	1	-0.6816434893265395
1115	2	-0.5318792584425296
1115	3	-0.4416008965934992
1115	4	-0.7308633225774912
1115	5	-0.404420705008512
1115	6	-0.3684477798459298
1115	7	-0.4341002699858265
1115	8	-0.20379351106426213
1115	9	-0.506935750479137
1115	10	-0.27022826063670635
1115	11	-0.516452326814651
1115	12	-0.6145818828756617
1115	13	-0.4907600720612848
1115	14	-0.431653245921197
1115	15	-1.5530085260368693
1115	16	-0.9103145198224922
1115	17	-0.7386604761818703
1115	18	-2.1680015883685613
1115	19	-1.163856931534582
1115	20	-0.96717971146861
1115	21	-0.8445435253252782
1115	22	-0.7298361299644611
1115	23	-0.3829664245239144
1115	24	-0.4139517595214741
1115	25	-0.5206665467767166
1116	1	-1.6833689198539545
1116	2	-1.0125424415870583
1116	3	-1.9541964140458035
1116	4	-1.2875265683344352
1116	5	-1.1976711639428501
1116	6	-0.800455102039848
1116	7	-1.509811917651561
1116	8	-3.133910482999628
1116	9	-1.7481353975318825
1116	10	-1.0172157946249172
1116	11	-0.8875832100363539
1116	12	-1.0502617607918197
1116	13	-2.112969484489561
1116	14	-1.9888276823078002
1116	15	-1.9509087700955803
1116	16	-1.3664703455320009
1116	17	-2.021816325500758
1116	18	-2.910348106430476
1116	19	-1.6772396012641835
1116	20	-1.2584362156148288
1116	21	-1.1391412755503225
1116	22	-0.8490126884278126
1116	23	-0.807286904640101
1116	24	-0.839363243763593
1116	25	-0.8185586270060625
1201	1	0.34745207330508165
1201	2	0.2933110969924222
1201	3	0.3932001494595063
1201	4	0.47550162832527576
1201	5	0.3072971340576629
1201	6	0.21655825863952316
1201	7	0.23457701872261794
1201	8	0.3219525869223716
1201	9	0.3071184893187322
1201	10	0.5823615777622351
1201	11	0.37340877643600123
1201	12	0.40267136415481986
1201	13	0.42558526741611047
1201	14	0.8471915267759158
1201	15	0.6722335181054717
1201	16	0.6543285095411946
1201	17	0.7495953811821181
1201	18	0.6084960520051226
1201	19	0.5759639840977329
1201	20	0.6486880514917885
1201	21	0.6401089746474184
1201	22	0.660903979181704
1201	23	0.8051642943719273
1201	24	0.7338069099001601
1201	25	0.8830824524630501
1202	1	0.3454671918966845
1202	2	0.3516353866648423
1202	3	0.42259260440582125
1202	4	0.4799917729627836
1202	5	0.358760928140768
1202	6	0.1787727128851202
1202	7	0.12477104980739284
1202	8	0.2662545070665132
1202	9	0.31176365930242866
1202	10	0.5074514945259025
1202	11	0.33594648379864367
1202	12	0.38399003636056506
1202	13	0.33149144223964555
1202	14	0.6646691812595478
1202	15	0.516744643046124
1202	16	0.47269683260056183
1202	17	0.5584418267510856
1202	18	0.6255024871969823
1202	19	0.43996578672379627
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2211	1	0.17482560483229292
2211	2	0.036261398375952325
2211	3	0.10607542583873908
2211	4	0.1958109308930247
2211	5	0.15498111847144713
2211	6	0.06792725912144704
2211	7	0.1619812455993923
2211	8	0.3002558182284787
2211	9	0.2086053672081576
2211	10	0.3861524837049046
2211	11	0.12622802623439355
2211	12	0.1319273016162228
2211	13	0.04325470937828868
2211	14	0.014274548312300794
2211	15	-0.03502983709602603
2211	16	0.020276711290165395
2211	17	0.12231920295293719
2211	18	0.11098019576834162
2211	19	0.12352924350068406
2211	20	0.11222681965774939
2211	21	0.16190049328621114
2211	22	0.061379181940530714
2211	23	0.17840927111127058
2211	24	0.2299222832452159
2211	25	0.08664087291789713
2212	1	0.0845064986101356
2212	2	0.08310290707894552
2212	3	0.06736353493096203
2212	4	0.056230488434444226
2212	5	0.11535859356719413
2212	6	0.09888860256152299
2212	7	0.0869171128405739
2212	8	0.2851712989484767
2212	9	0.08569106031607987
2212	10	0.29091370976218656
2212	11	0.16268870908242405
2212	12	0.20185644899188304
2212	13	0.1108766997060826
2212	14	0.12289530822587125
2212	15	0.052066669211221495
2212	16	-0.03662053202960891
2212	17	0.06893168195274768
2212	18	0.15103053633873031
2212	19	0.2063900811595829
2212	20	0.13958593278614018
2212	21	0.15425798124810086
2212	22	-0.47568944643632705
2212	23	0.15753574073784904
2212	24	0.10245994355780397
2212	25	0.10649248932607236
2213	1	-0.06504967053170319
2213	2	-0.7755888433137912
2213	3	-0.30261291028837434
2213	4	-0.04493424071281993
2213	5	0.020185677500791144
2213	6	-0.059937621160536025
2213	7	-0.06769039615679873
2213	8	0.03965518754056774
2213	9	-0.28753115844995847
2213	10	-1.7112444252120156
2213	11	-0.5920490198667778
2213	12	-0.24466327029428214
2213	13	-0.2528693800156816
2213	14	-0.021308114017436708
2213	15	-0.10773200375014369
2213	16	-0.12788181662983789
2213	17	-0.21826143629439798
2213	18	-0.10446307530776977
2213	19	-0.06408283614045018
2213	20	-0.06380569679402726
2213	21	-0.037445639473531855
2213	22	0.01306873308481471
2213	23	-0.8557070213873246
2213	24	-1.447855922698153
2213	25	-1.411071613500006
2214	1	0.041769103326856794
2214	2	-0.1710567697711589
2214	3	-0.0010740148380747883
2214	4	-0.006846725215162053
2214	5	-0.03332408920681473
2214	6	-0.13388373141963683
2214	7	-0.07443395863023028
2214	8	-0.007764219738881195
2214	9	-0.3069020073788806
2214	10	-0.03299148383968031
2214	11	-0.10413474517678623
2214	12	-0.04709921820256113
2214	13	-0.09194541598395745
2214	14	-0.03461896228042605
2214	15	-0.1502597330518327
2214	16	-0.2364780470508805
2214	17	-0.18740616141049316
2214	18	-0.06496730496886727
2214	19	-0.7013935590511338
2214	20	-0.3848045588301048
2214	21	-0.2837423601396267
2214	22	-0.6249539517657752
2214	23	-1.8423344762849752
2214	24	-1.3118065573921456
2214	25	-1.6962677017245298
2215	1	-0.5152274347433909
2215	2	-0.31430987123571086
2215	3	-1.1796136806484085
2215	4	-0.9535360517856546
2215	5	-0.34920759856426997
2215	6	-0.2740561984654377
2215	7	-0.22003900587495906
2215	8	-0.15351488760587273
2215	9	-0.338061892908307
2215	10	-1.4386222650061953
2215	11	-0.5278833197839319
2215	12	-0.564352509963463
2215	13	-0.5821410845462722
2215	14	-0.767760242682094
2215	15	-1.0063727398073539
2215	16	-0.675272563588365
2215	17	-0.5616560205180079
2215	18	-0.47622324624048673
2215	19	-1.1454628561988827
2215	20	-1.5040518050223426
2215	21	-0.7536616891254155
2215	22	-0.4468761498349991
2215	23	-0.7591595134293598
2215	24	-1.97163789627335
2215	25	-0.863100160426562
2216	1	-0.5712889561919461
2216	2	-0.4576713630090943
2216	3	-1.4323699054593888
2216	4	-0.8347547730140548
2216	5	-0.6389018732853344
2216	6	-0.5929540473959847
2216	7	-0.36668154688122456
2216	8	-0.47645519485803334
2216	9	-0.3517495427108557
2216	10	-0.4262007761242401
2216	11	-0.45808735814352985
2216	12	-0.3000284063692149
2216	13	-1.102298682531405
2216	14	-0.8051865683632897
2216	15	-1.2703166359797606
2216	16	-0.9703147403762955
2216	17	-1.974372150875702
2216	18	-0.9958061502983782
2216	19	-0.7066513239708883
2216	20	-0.6857811522446724
2216	21	-0.4482689547232752
2216	22	-0.5419998141352593
2216	23	-0.5210962419066192
2216	24	-0.5708646779883679
2216	25	-0.5523111508924643
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5377.0
3	554.0
4	708.0
5	644.0
6	410.0
7	313.0
8	450.0
9	896.0
10	1899.0
11	3501.0
12	6367.0
13	9762.0
14	13325.0
15	17428.0
16	20367.0
17	21757.0
18	22483.0
19	23161.0
20	24013.0
21	25851.0
22	27856.0
23	29726.0
24	31927.0
25	34222.0
26	36183.0
27	40789.0
28	45938.0
29	53961.0
30	66020.0
31	84723.0
32	113786.0
33	159824.0
34	232597.0
35	371516.0
36	671638.0
37	2291858.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.379528655908096	20.380508669436303	12.920685875527118	23.31927679912848
2	14.735929948216661	21.08722711839613	39.88514543799535	24.29169749539186
3	18.78767481213195	26.34655775416133	29.470610551924914	25.3951568817818
4	12.015417325395644	18.225423729947853	37.72941558851499	32.02974335614151
5	12.34996598288054	39.44691666999511	35.16845781843717	13.034659528687175
6	31.245490661724972	37.719935830513336	17.580882613686622	13.453690894075073
7	25.91066869349719	33.14800144875562	22.89123092486632	18.050098932880864
8	24.403780028485166	37.238299500226965	21.219798640876103	17.138121830411762
9	24.23181643987546	17.27693064744016	21.652794191718094	36.83845872096629
10	14.389241598456609	30.924291911902003	34.05091555144683	20.635550938194562
11	31.443251142399852	26.122504875607333	22.668406956639657	19.765837025353157
12	20.870909151335777	28.618090951044874	31.308070071191356	19.20292982642799
13	27.039024989833234	23.691900249467793	25.95694198842093	23.312132772278044
14	19.98949422851726	24.414208442536108	28.016974293202473	27.579323035744157
15	24.185620257884967	30.04774928868971	23.586350960951926	22.180279492473392
16	20.64862246237346	31.307462454870343	27.139865519371043	20.904049563385154
17	20.793066777057696	30.616961558226514	28.357142347332058	20.232829317383732
18	20.949816743276422	29.59830286786958	29.372510777707987	20.079369611146006
19	20.94719407316737	29.85120466852267	29.52514054949354	19.676460708816425
20	22.037835518227674	28.963834601089506	28.641364180579863	20.356965700102954
21	21.384673065286577	29.47126297319703	28.640362782255487	20.503701179260904
22	20.798154810111253	29.251684141711692	28.098975764373833	21.851185283803222
23	20.148417956922213	29.735919086116542	28.622249087443695	21.493413869517553
24	20.792241770087127	30.13213993796557	28.173709787376815	20.90190850457049
25	21.337774125448586	28.57567637983986	27.12942986189556	22.957119632816
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10030.0
1	10030.0
2	9043.5
3	8057.0
4	8057.0
5	8057.0
6	10683.5
7	13310.0
8	13310.0
9	13310.0
10	17728.0
11	22146.0
12	22146.0
13	22146.0
14	34106.0
15	46066.0
16	46066.0
17	46066.0
18	69880.0
19	93694.0
20	93694.0
21	93694.0
22	131465.5
23	169237.0
24	169237.0
25	169237.0
26	215892.5
27	262548.0
28	262548.0
29	262548.0
30	312025.0
31	361502.0
32	361502.0
33	361502.0
34	399489.5
35	437477.0
36	437477.0
37	437477.0
38	461232.0
39	484987.0
40	484987.0
41	484987.0
42	489938.0
43	494889.0
44	494889.0
45	494889.0
46	565681.0
47	636473.0
48	636473.0
49	636473.0
50	576634.0
51	516795.0
52	516795.0
53	516795.0
54	438710.5
55	360626.0
56	360626.0
57	360626.0
58	307454.5
59	254283.0
60	254283.0
61	254283.0
62	210594.0
63	166905.0
64	166905.0
65	166905.0
66	128404.5
67	89904.0
68	89904.0
69	89904.0
70	64815.5
71	39727.0
72	39727.0
73	39727.0
74	27811.0
75	15895.0
76	15895.0
77	15895.0
78	10485.5
79	5076.0
80	5076.0
81	5076.0
82	3357.0
83	1638.0
84	1638.0
85	1638.0
86	1015.5
87	393.0
88	393.0
89	393.0
90	266.0
91	139.0
92	139.0
93	139.0
94	80.0
95	21.0
96	21.0
97	21.0
98	16.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2738304877967332
2	0.16420924211290278
3	0.18324380041096835
4	0.2021670455026125
5	0.22848148750064007
6	0.1798598789357567
7	0.17770040273118085
8	0.19579993009530638
9	0.19706890064851074
10	0.2055064416952556
11	0.18446824568160416
12	0.19059047203478313
13	0.20230062135031826
14	0.1910134622191846
15	0.18262044645500833
16	0.18609341849535713
17	0.18642735811462144
18	0.18927697619901018
19	0.18602663057150426
20	0.18518065020270133
21	0.18386715436692838
22	0.18676129773388575
23	0.19023426977423455
24	0.20388126888150263
25	0.19693532480080503
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4491830.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.98105690516182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.77473933637391	64.93667985680024
2	9.27546357471653	13.724171970857185
3	1.723773298515503	3.8257971146722176
4	0.4890391344187649	1.447185281291429
5	0.20427608481046827	0.7556280327363457
6	0.11007598419141632	0.48861225902141175
7	0.07553541352116139	0.3911732808244779
8	0.05182703466522041	0.3067375040634772
9	0.03719328180733702	0.2476438468090488
>10	0.2250452910259718	3.1097695161058425
>50	0.018494948633712205	0.9523098613823056
>100	0.011777647496248425	1.6169948373605383
>500	0.0010005057340318902	0.5239161610735666
>1k	0.0013036888940968607	2.595378415971738
>5k	1.8191007824602568E-4	0.9260829483681777
>10k+	2.728651173690385E-4	4.15191911266199
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	45921	1.0223227504157548	No Hit
CCCATGTACTCTGCGTTGATACCAC	41578	0.9256360993180952	No Hit
GAGTACATGGGAAGCAGTGGTATCA	21627	0.48147414305528036	No Hit
CATGTACTCTGCGTTGATACCACTG	15934	0.35473292622383307	No Hit
GCGTTGATACCACTGCTTCCCATGT	13812	0.3074916014185755	No Hit
GCTTCCCATGTACTCTGCGTTGATA	12378	0.2755669738169076	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	11856	0.2639458750665096	No Hit
GTATCAACGCAGAGTACATGGGAAG	11659	0.25956013473350503	No Hit
TATCAACGCAGAGTACATGGGAAGC	10342	0.23024023616209874	No Hit
GGTATCAACGCAGAGTACATGGGAA	9557	0.21276406275393325	No Hit
ACTCTGCGTTGATACCACTGCTTCC	9206	0.20494987566314843	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6171	0.13738275936533662	No Hit
GTGGTATCAACGCAGAGTACATGGG	6164	0.1372269208763466	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5141	0.11445223884252076	No Hit
CAGTGGTATCAACGCAGAGTACATG	5049	0.11240407584436633	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4757	0.10590338458935444	No Hit
GCAGTGGTATCAACGCAGAGTACAT	4696	0.10454536347101293	No Hit
ACATGGGAAGCAGTGGTATCAACGC	4577	0.10189610915818274	No Hit
ATACCACTGCTTCCCATGTACTCTG	4514	0.10049356275727264	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.226264128428725E-5	0.0
4	0.0	0.0	0.0	2.226264128428725E-5	0.0
5	0.0	0.0	0.0	2.226264128428725E-5	0.0
6	0.0	0.0	0.0	2.226264128428725E-5	0.0
7	0.0	0.0	0.0	8.9050565137149E-5	0.0
8	0.0	0.0	0.0	1.1131320642143626E-4	0.0
9	0.0	0.0	0.0	2.0036377155858526E-4	0.0
10	0.0	0.0	0.0	2.0036377155858526E-4	0.0
11	0.0	0.0	0.0	2.4488905412715976E-4	0.0
12	0.0	0.0	0.0	2.67151695411447E-4	0.0
13	0.0	0.0	0.0	2.8941433669573427E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTAC	50	0.0014932129	13.308379	3
GTCTACG	65	5.3970303E-5	13.169337	1
TAGGCGT	130	3.2787284E-8	10.959999	6
AATACCG	90	9.4530915E-5	10.563267	5
GACCGGG	85	6.605273E-4	10.057635	7
AGTACCG	135	6.387327E-7	9.85905	5
CTAGTAC	265	0.0	9.685344	3
CGAATAC	90	0.0011079753	9.505984	3
GACCGTG	110	6.8512585E-5	9.4988785	7
GTAGGAC	185	6.6374923E-9	9.249066	3
GCACCGT	135	6.866745E-6	9.146865	6
GTCTTGC	750	0.0	9.130741	1
ATACCGT	115	1.1132316E-4	9.085681	6
TATACCG	95	0.0018127672	9.006576	5
CCATGCG	95	0.0018266288	8.999039	9
GCCCTAG	275	0.0	8.992397	1
CTTGGAC	520	0.0	8.957562	3
GTCTAGG	490	0.0	8.928871	1
CTAGCAC	430	0.0	8.842776	3
GTATAGA	755	0.0	8.692345	1
>>END_MODULE