FastQCFastQC Report
Wed 25 May 2016
SRR1295022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4491830
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC468471.0429379562450047No Hit
GTACATGGGAAGCAGTGGTATCAAC442130.9842981591021921No Hit
GAGTACATGGGAAGCAGTGGTATCA230710.5136213970697912No Hit
CATGTACTCTGCGTTGATACCACTG183680.4089201951097883No Hit
GTATCAACGCAGAGTACTTTTTTTT152350.33917133996611626No Hit
GCGTTGATACCACTGCTTCCCATGT144130.32087144883043217No Hit
ACGCAGAGTACATGGGAAGCAGTGG139730.31107588666534575No Hit
GGTATCAACGCAGAGTACTTTTTTT137580.306289418789224No Hit
GCTTCCCATGTACTCTGCGTTGATA136620.3041522052259324No Hit
GTATCAACGCAGAGTACATGGGAAG119510.26606082598851694No Hit
TATCAACGCAGAGTACATGGGAAGC110210.2453565695941298No Hit
TATCAACGCAGAGTACTTTTTTTTT108160.2407927281308509No Hit
GGTATCAACGCAGAGTACATGGGAA104260.23211029802997887No Hit
ACTCTGCGTTGATACCACTGCTTCC103980.23148694407401885No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65800.1464881796506101No Hit
GTGGTATCAACGCAGAGTACATGGG62810.13983164990660824No Hit
CAGTGGTATCAACGCAGAGTACATG61650.1372491835176309No Hit
ACATGGGAAGCAGTGGTATCAACGC58320.12983572396996326No Hit
CATGGGAAGCAGTGGTATCAACGCA54040.12030731350028831No Hit
GGGAAGCAGTGGTATCAACGCAGAG50380.11215918679023916No Hit
GCAGTGGTATCAACGCAGAGTACAT50290.11195882301868057No Hit
ATACCACTGCTTCCCATGTACTCTG47430.10559170761137442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG600.00588174511.0825357
TATTCCG1451.4473699E-810.482125
GTACCGC951.6465907E-49.999286
AGGACCG1054.111657E-59.9517755
AGGAGCG900.00111499799.4994215
TGGACCG800.00452052769.4994215
AAGCCCG900.00111499799.4994215
GAGCGTC900.00111511149.4993157
TAGACTG6650.09.4279975
TGTCGCC3500.09.22790610
CTGTCGC3650.09.10893259
GACTGTC3450.09.0863027
TCTGTCG3700.08.9858398
AGAACCG850.0074430418.9406325
TAAGCCG850.0074430418.9406325
TCCGTAC850.0074437218.9405328
GAACCGT850.0074437218.9405326
TACACTG10700.08.8779635
TAGACAG7400.08.8575685
GTTCTAG3900.08.7801311
Produced by FastQC (version 0.11.5)