FastQCFastQC Report
Wed 25 May 2016
SRR1295021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4015909
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC280470.6983972993411952No Hit
CCCATGTACTCTGCGTTGATACCAC259980.6473752268788959No Hit
GAGTACATGGGAAGCAGTGGTATCA136370.33957442760779694No Hit
CATGTACTCTGCGTTGATACCACTG102060.2541392247682903No Hit
GCGTTGATACCACTGCTTCCCATGT83190.20715110825469402No Hit
GCTTCCCATGTACTCTGCGTTGATA79220.197265426084107No Hit
ACGCAGAGTACATGGGAAGCAGTGG73160.18217544272044012No Hit
GTATCAACGCAGAGTACATGGGAAG72140.179635544530516No Hit
TATCAACGCAGAGTACATGGGAAGC65560.16325071110924078No Hit
GGTATCAACGCAGAGTACATGGGAA64170.159789477301403No Hit
ACTCTGCGTTGATACCACTGCTTCC59240.14751330271676974No Hit
GTATCAACGCAGAGTACTTTTTTTT53090.13219921068928603No Hit
GGTATCAACGCAGAGTACTTTTTTT45600.11354838966719613No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN43350.10794567307177529No Hit
GTGGTATCAACGCAGAGTACATGGG41330.10291567861721966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC2550.010.4426171
CTAGACA5050.010.3518674
GTCCTAA2650.010.0485571
AGTACGG951.6390324E-410.0037793
TAGTACG900.00111047649.5035912
GTCCTAG4500.09.29890251
GTATTAG6650.09.2957241
TTAGACT4200.09.0522824
TACACGC850.00741145438.9452265
TAGACAG6200.08.8911225
TACACTG9500.08.8039865
GTCTAGG4650.08.7944161
TCTATAC6700.08.7943673
GTAAGAC4350.08.7389343
AGACGTG1201.756373E-48.7122775
GTGTAGA5350.08.7103151
GTCTTGC7700.08.6456741
TCTAGAC4100.08.5764113
ATACCGT1000.00291065878.548646
TAGACTG5400.08.4482695
Produced by FastQC (version 0.11.5)