FastQCFastQC Report
Wed 25 May 2016
SRR1295021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4015909
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC287850.7167742097741757No Hit
GTACATGGGAAGCAGTGGTATCAAC281390.7006881879046561No Hit
GAGTACATGGGAAGCAGTGGTATCA147820.3680860298378275No Hit
GTATCAACGCAGAGTACTTTTTTTT135840.33825467658754216No Hit
GGTATCAACGCAGAGTACTTTTTTT125000.3112620330789368No Hit
CATGTACTCTGCGTTGATACCACTG114210.284393894383563No Hit
TATCAACGCAGAGTACTTTTTTTTT95610.2380781038614172No Hit
ACGCAGAGTACATGGGAAGCAGTGG91480.2277940062884891No Hit
GCGTTGATACCACTGCTTCCCATGT91480.2277940062884891No Hit
GCTTCCCATGTACTCTGCGTTGATA87500.21788342315525577No Hit
GTATCAACGCAGAGTACATGGGAAG79000.19671760490588805No Hit
GGTATCAACGCAGAGTACATGGGAA71760.17868930794995602No Hit
TATCAACGCAGAGTACATGGGAAGC71290.17751896270557924No Hit
ACTCTGCGTTGATACCACTGCTTCC68930.1716423355210489No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56730.1412631610925447No Hit
GTGGTATCAACGCAGAGTACATGGG45160.11245274731075827No Hit
CAGTGGTATCAACGCAGAGTACATG40660.10124731411991657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG701.0925796E-412.21326817
CGGGTTC2001.8189894E-1210.4491318
GCACCGT1002.403177E-510.449136
CTAGGAC4600.010.3253563
AGGACCG750.002650688410.1326175
TATAACG750.002650688410.1326172
GTAGGAC2250.010.1326163
AACGCGA750.002650962210.13249116
CGCGAGT856.602839E-410.05798718
TAGACAG7400.010.0128055
TAGGACT4950.09.9790924
CTTACAC6250.09.7273123
TTAGACT3750.09.372674
GTCCTAC2800.09.1740011
CACCGTC1151.1124625E-49.08627
TCTATAC5550.09.071433
CCAAGCG950.00182615028.9992519
GTCTAAG4450.08.9792971
CAACGCG850.00744411638.94043315
TTACACC3750.08.8660394
Produced by FastQC (version 0.11.5)