FastQCFastQC Report
Wed 25 May 2016
SRR1295020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3730172
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC297490.7975235458311306No Hit
CCCATGTACTCTGCGTTGATACCAC276410.7410114064445286No Hit
GAGTACATGGGAAGCAGTGGTATCA146240.39204626489073424No Hit
CATGTACTCTGCGTTGATACCACTG111130.29792191888202474No Hit
GCTTCCCATGTACTCTGCGTTGATA89520.23998893348617703No Hit
GCGTTGATACCACTGCTTCCCATGT87630.2349221430003764No Hit
GTATCAACGCAGAGTACATGGGAAG78280.20985627472406096No Hit
ACGCAGAGTACATGGGAAGCAGTGG75980.20369033921224006No Hit
GGTATCAACGCAGAGTACATGGGAA71450.19154612709547977No Hit
TATCAACGCAGAGTACATGGGAAGC68850.18457593912559528No Hit
ACTCTGCGTTGATACCACTGCTTCC63150.16929514242238697No Hit
GTATCAACGCAGAGTACTTTTTTTT56780.15221818189616992No Hit
GGTATCAACGCAGAGTACTTTTTTT49560.13286250607210606No Hit
GTGGTATCAACGCAGAGTACATGGG43260.11597320445277054No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN40260.10793067987213457No Hit
TATCAACGCAGAGTACTTTTTTTTT38440.10305154829321544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG600.00585518811.0891935
ACTCGCG600.005881021311.0826368
TACACCG951.3535449E-511.0058155
TCCGTAC700.001486351210.8622983
CACCGTC750.002650143310.1328327
GTGTAGG4050.010.0954171
TCTGTCG2500.09.8793788
CTACCCT4200.09.7318554
GTCTTAC3850.09.6319611
GCTACCC1701.6543709E-89.5045123
CCGTTCA800.00452029339.4994029
GTCCTAG4750.09.4083851
GTCTTAG3750.09.3816951
GTATTAG5600.09.1688851
GTCTAGG3950.09.1473931
CTGTCGC2501.8189894E-129.1194269
TTATACT7750.09.0762514
CCTACAC4300.09.0624413
GTGTTAG6100.09.0408451
TACACTG8350.08.9927765
Produced by FastQC (version 0.11.5)