FastQCFastQC Report
Wed 25 May 2016
SRR1295020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3730172
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC301440.8081128698623011No Hit
CCCATGTACTCTGCGTTGATACCAC298150.7992929012388705No Hit
GAGTACATGGGAAGCAGTGGTATCA162720.4362265332536945No Hit
GTATCAACGCAGAGTACTTTTTTTT147070.3942713633580435No Hit
GGTATCAACGCAGAGTACTTTTTTT140500.3766582345264508No Hit
CATGTACTCTGCGTTGATACCACTG121570.3259099044226379No Hit
TATCAACGCAGAGTACTTTTTTTTT102400.27451817235237413No Hit
GCTTCCCATGTACTCTGCGTTGATA97830.26226672657453864No Hit
ACGCAGAGTACATGGGAAGCAGTGG95810.25685142669024374No Hit
GCGTTGATACCACTGCTTCCCATGT94320.2528569728151946No Hit
GTATCAACGCAGAGTACATGGGAAG85210.22843450650533006No Hit
GGTATCAACGCAGAGTACATGGGAA79130.21213499002190783No Hit
TATCAACGCAGAGTACATGGGAAGC78270.20982946630879218No Hit
ACTCTGCGTTGATACCACTGCTTCC69980.18760529005096815No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60810.16302197324949091No Hit
GTGGTATCAACGCAGAGTACATGGG46090.12355998597383715No Hit
ACATGGGAAGCAGTGGTATCAACGC43710.11717958313986593No Hit
CAGTGGTATCAACGCAGAGTACATG40700.10911025014396118No Hit
CATGGGAAGCAGTGGTATCAACGCA39500.1058932403117068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT500.001501071513.2990144
GTACCGT855.3312848E-511.1756426
CTCCGTA750.002650687210.13258312
CAAGACT6100.09.8107484
CTGCTCG800.0045207139.4992969
TACACTG9600.09.4003455
GTCCTAC2950.09.3512021
TAGGACT5000.09.309314
CTAGACT4500.09.28824
GTGTTAG6650.09.15481951
ACAGCGT1356.8598492E-69.147478
CAGGACT5400.09.147474
GTCTAAG3550.09.1104961
CGCGATC1151.11229536E-49.08628417
GACAGTG5050.09.0290357
GTCCTAG3500.08.9688631
TGCACCG850.00744335358.9405145
CTTACAC6650.08.8564873
CTAATAC5150.08.8537133
TCTAGAC4100.08.8042263
Produced by FastQC (version 0.11.5)