FastQCFastQC Report
Wed 25 May 2016
SRR1295019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924325
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC426200.7194068522574302No Hit
CCCATGTACTCTGCGTTGATACCAC413550.698054208707321No Hit
GAGTACATGGGAAGCAGTGGTATCA188000.3173357302308702No Hit
CATGTACTCTGCGTTGATACCACTG155260.26207205040236653No Hit
GCGTTGATACCACTGCTTCCCATGT117280.19796348107168327No Hit
ACGCAGAGTACATGGGAAGCAGTGG96930.16361357623020345No Hit
GTATCAACGCAGAGTACATGGGAAG95640.16143611297489588No Hit
TATCAACGCAGAGTACATGGGAAGC89870.15169660678642716No Hit
GCTTCCCATGTACTCTGCGTTGATA87260.14729104159545603No Hit
ACTCTGCGTTGATACCACTGCTTCC85700.14465783021694456No Hit
GGTATCAACGCAGAGTACATGGGAA78740.1329096563743549No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN70840.11957480388061087No Hit
GTATCAACGCAGAGTACTTTTTTTT62070.10477142965654314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC1302.57387E-911.7057921
TAAGGCG909.4697956E-510.5615565
TACGACC1659.222276E-1010.3701434
TATACTG10900.010.2030455
CGTACCC1409.62973E-810.1840993
GGGTACG1451.59298E-79.8389211
GTACGAC1752.5556801E-99.7767363
CGTAATA800.0044984179.505162
ACCGAGC1002.7614887E-49.4987278
CCGAGCG800.00452424039.4985679
TTACACC5850.09.4247174
TAGACAG7950.09.3260555
GTGTTAG9800.09.2198051
GCACCGC1151.1135202E-49.0855856
AATACCG1054.4707162E-49.0527635
AGTGCGC850.00744912078.93982710
ACACCGT2356.730261E-118.8922756
CCTATAC5350.08.8833273
TAATACG1401.0778222E-58.8269664
GGACCGT1401.0884774E-58.8200976
Produced by FastQC (version 0.11.5)