FastQCFastQC Report
Wed 25 May 2016
SRR1295019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924325
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC450720.7607955336683926No Hit
GTACATGGGAAGCAGTGGTATCAAC420190.7092622366261135No Hit
GAGTACATGGGAAGCAGTGGTATCA199100.33607204196258644No Hit
GTATCAACGCAGAGTACTTTTTTTT171190.28896118967139717No Hit
CATGTACTCTGCGTTGATACCACTG169940.28685124465656425No Hit
GGTATCAACGCAGAGTACTTTTTTT140220.2366851919838969No Hit
TATCAACGCAGAGTACTTTTTTTTT123130.2078380237411013No Hit
GCGTTGATACCACTGCTTCCCATGT122980.20758483033932137No Hit
ACGCAGAGTACATGGGAAGCAGTGG116800.19715326218598742No Hit
GTATCAACGCAGAGTACATGGGAAG101400.17115873960324593No Hit
TATCAACGCAGAGTACATGGGAAGC95850.1617905837373878No Hit
GCTTCCCATGTACTCTGCGTTGATA95690.1615205107754892No Hit
ACTCTGCGTTGATACCACTGCTTCC91090.1537559131209041No Hit
GGTATCAACGCAGAGTACATGGGAA85440.1442189616538593No Hit
ACGCAGAGTACTTTTTTTTTTTTTT71980.1214990737341385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA500.001501426113.29881312
CCTCGCG550.003072251612.08982859
CGCGCGC600.00588287311.08234312
AGGACCG1355.7045327E-810.55479055
CGTACCC1002.40342E-510.4492443
GTACGAC1201.5273672E-610.2909213
GTCGTAC951.621865E-410.0144051
GTACCGC951.6467979E-49.9992766
ACCGTCC951.6467979E-49.9992768
TACGACC1252.5759418E-69.8792844
TACACCG1356.4593587E-79.8511385
TCGTACC1002.7593155E-49.4994732
TTACGCT800.00452131459.4993124
TATACCG1304.248126E-69.4993125
TACCGGT800.00452131459.4993127
TACCGAT800.00452131459.4993127
TGTACCG1201.7038621E-59.4993115
TTGGACT6300.09.348534
TTAGGCT4450.09.1791114
ATACCGA1151.1125686E-49.0862996
Produced by FastQC (version 0.11.5)