FastQCFastQC Report
Wed 25 May 2016
SRR1295018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124403
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC62920.5595858424426118No Hit
GTACATGGGAAGCAGTGGTATCAAC62770.5582518011780474No Hit
GAGTACATGGGAAGCAGTGGTATCA31740.2822831315818261No Hit
CATGTACTCTGCGTTGATACCACTG26730.23772615334537528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA26480.23550275123776793No Hit
GCGTTGATACCACTGCTTCCCATGT22980.20437512173126537No Hit
ACGCAGAGTACATGGGAAGCAGTGG21820.1940585359519674No Hit
ACTCTGCGTTGATACCACTGCTTCC18860.16773345499789666No Hit
GTATCAACGCAGAGTACATGGGAAG18820.1673777106606795No Hit
GTGGTATCAACGCAGAGTACATGGG18580.16524324463737644No Hit
GTATCAACGCAGAGTACTTTTTTTT15970.14203092663395597No Hit
TATCAACGCAGAGTACATGGGAAGC15880.14123050187521735No Hit
GGTATCAACGCAGAGTACATGGGAA15850.14096369362230446No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN13130.11677307869153676No Hit
GTACTCTGCGTTGATACCACTGCTT11600.10316585779297993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT701.07659485E-412.2309391
GTCTAAG657.929202E-411.7082481
GTACTGG1408.1599865E-910.8719461
CTAGCAC909.459278E-510.5609863
TCTAAGG1151.019073E-59.9181423
GTCCAGA1401.037437E-69.5129531
GGTTTAC800.00446333749.5129521
CTTAAGG900.00110778049.5048873
GGACAGT1106.838357E-59.4989526
CTGTACC1752.636989E-89.2341424
GTGTAAG1252.706995E-59.1324341
GTATTAG850.0073494648.9533671
GGACTGC850.0074388028.9401896
TTAGATG1606.67791E-78.9108313
TAAGACA1304.332637E-58.7745244
GTCCAGG2502.5465852E-118.7519161
GTATATA1651.0275598E-68.6481391
GGATAGG1000.00286752528.5616571
GTCTCGA1451.6947126E-58.51402311
GAACAGT1356.79835E-58.4435136
Produced by FastQC (version 0.11.5)