FastQCFastQC Report
Wed 25 May 2016
SRR1295018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124403
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC71810.6386500213891283No Hit
GTACATGGGAAGCAGTGGTATCAAC60500.5380633100409729No Hit
GTATCAACGCAGAGTACTTTTTTTT51970.4622008301294109No Hit
GGTATCAACGCAGAGTACTTTTTTT37450.3330656357195774No Hit
TATCAACGCAGAGTACTTTTTTTTT33270.2958903524803829No Hit
GAGTACATGGGAAGCAGTGGTATCA33070.2941116307942971No Hit
CATGTACTCTGCGTTGATACCACTG30640.2725001623083539No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28010.2491099721363248No Hit
ACGCAGAGTACATGGGAAGCAGTGG25560.22732063148177298No Hit
GCGTTGATACCACTGCTTCCCATGT25390.2258087180486No Hit
ACTCTGCGTTGATACCACTGCTTCC21030.18703258529192823No Hit
GTATCAACGCAGAGTACATGGGAAG19140.17022366535841688No Hit
GTGGTATCAACGCAGAGTACATGGG18590.16533218072168074No Hit
GGTATCAACGCAGAGTACATGGGAA17390.15465985060516557No Hit
TATCAACGCAGAGTACATGGGAAGC16290.14487688133169335No Hit
GATACCACTGCTTCCCATGTACTCT13140.11686201477584106No Hit
GCTTCCCATGTACTCTGCGTTGATA13080.11632839827001527No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12790.11374925182519079No Hit
CAGTGGTATCAACGCAGAGTACATG11790.10485564339476147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC752.0383525E-411.4206091
TAGGATA1104.9698247E-711.2260644
TTAGGAC600.00587865511.082143
CTTAGAC803.7726777E-410.686353
GACTGGT1002.3996045E-510.4488747
GGCTAAC1105.9104386E-610.3823731
ACTTTGC1409.68721E-810.1774768
ATCCTAC856.481375E-410.0770091
CAGTGTA1054.1063195E-59.951319
GTATAGG800.0044470399.5171761
AGTGCCC800.004517829.4989788
CTTATAC900.00111410529.4989783
GTAACAC800.004517829.4989783
TCAGCAC1151.1108606E-49.08597853
GAACAGT1151.1108606E-49.08597856
TTAAGAC950.00182442888.9990313
CCAGCGG850.0074386678.9402138
CCTCTAG1401.0591009E-58.8373771
GTCATAG1304.271904E-58.7850851
CCTCTAT1201.727853E-48.7240771
Produced by FastQC (version 0.11.5)