FastQCFastQC Report
Wed 25 May 2016
SRR1295017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6175917
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC410670.6649538845810266No Hit
CCCATGTACTCTGCGTTGATACCAC392970.6362941729948767No Hit
GAGTACATGGGAAGCAGTGGTATCA190760.3088772080324266No Hit
CATGTACTCTGCGTTGATACCACTG148940.24116256743735384No Hit
GCGTTGATACCACTGCTTCCCATGT125010.20241528504997716No Hit
GTATCAACGCAGAGTACATGGGAAG105380.17063053146601548No Hit
ACGCAGAGTACATGGGAAGCAGTGG104910.1698695108758748No Hit
GCTTCCCATGTACTCTGCGTTGATA93660.15165359249484733No Hit
ACTCTGCGTTGATACCACTGCTTCC91510.14817232809313985No Hit
TATCAACGCAGAGTACATGGGAAGC91140.14757322677749718No Hit
GGTATCAACGCAGAGTACATGGGAA87810.14218131493671304No Hit
GTATCAACGCAGAGTACTTTTTTTT72590.1175372013581141No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN71610.1159503924680335No Hit
ATTCCATTCCATTCCATTCCATTCC62300.10087570801226767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCG1209.986252E-911.8789742
GAACCGT658.034155E-411.6914736
TTAGACG907.4319705E-611.6165064
GACCGAC600.00588035411.0829937
TAGACGG1408.312782E-910.8619235
AGACCGT1303.8516737E-710.230046
AGGACCG1252.5591853E-69.8843495
GTATTAG8900.09.0820431
ACCGTCC1054.4987357E-49.0471218
ACCGACC950.0018259728.9995038
CGTAGTC850.00741868.9443143
GCCGCGG1606.717182E-78.9093743
CACGACA1401.0800732E-58.8255274
TACACCG1304.3508575E-58.7730915
CTAGGAC6650.08.7173433
GTGTAGA9850.08.6888221
GTGTTAG7700.08.6449391
GTAGGAC4950.08.6393943
GCACCGT2201.6116246E-98.6357486
ACACCGT2106.4010237E-98.59462456
Produced by FastQC (version 0.11.5)