FastQCFastQC Report
Wed 25 May 2016
SRR1295017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6175917
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC436870.7073767344995083No Hit
GTACATGGGAAGCAGTGGTATCAAC415250.672369787353036No Hit
GAGTACATGGGAAGCAGTGGTATCA211070.3417630126829749No Hit
GTATCAACGCAGAGTACTTTTTTTT196220.31771800042001863No Hit
CATGTACTCTGCGTTGATACCACTG165850.26854311675496934No Hit
GGTATCAACGCAGAGTACTTTTTTT165750.2683811974804713No Hit
GCGTTGATACCACTGCTTCCCATGT137540.22270377014457932No Hit
TATCAACGCAGAGTACTTTTTTTTT135300.21907677839582365No Hit
ACGCAGAGTACATGGGAAGCAGTGG127950.20717571172021904No Hit
GTATCAACGCAGAGTACATGGGAAG112510.18217537573772447No Hit
TATCAACGCAGAGTACATGGGAAGC104650.16944852076217992No Hit
GCTTCCCATGTACTCTGCGTTGATA104570.16931898534258152No Hit
ACTCTGCGTTGATACCACTGCTTCC101610.16452617481744009No Hit
GGTATCAACGCAGAGTACATGGGAA96570.15636544338273978No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80170.12981068236506416No Hit
GTGGTATCAACGCAGAGTACATGGG62430.10108620306911507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA550.003071721612.0901217
CGGGACT658.034288E-411.6914514
GTCGTAC907.360859E-611.6265451
CGTGCTA803.7766536E-410.68671710
AACCGTG1451.4479156E-810.4820767
GACTGCG1606.193659E-910.0930927
ACGCAGT951.6468379E-49.99926710
TAACGCT1002.7598487E-49.4993044
GTCTTAG6150.09.1259361
AACTGCG1151.1125016E-49.0863657
TCGGAAC1151.1125997E-49.0862913
TACCGTC950.00182613968.99941357
CCGGACT850.00744445768.9405214
TTACACT12400.08.8098384
GACCGTG2054.154572E-98.8043047
GGACCGT1304.3771626E-58.7685886
TAGACCG1201.7668301E-48.7076955
TAGACAG9750.08.6711595
TCGTACC1107.153394E-48.6359412
GTATAGG7000.08.5613641
Produced by FastQC (version 0.11.5)