FastQCFastQC Report
Wed 25 May 2016
SRR1295016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673888
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC86540.5169999426484926No Hit
CCCATGTACTCTGCGTTGATACCAC76050.4543314725955381No Hit
GAGTACATGGGAAGCAGTGGTATCA41560.24828423407061884No Hit
GCTTCCCATGTACTCTGCGTTGATA32110.19182884398478275No Hit
GTATCAACGCAGAGTACTTTTTTTT31610.18884178630828347No Hit
CATGTACTCTGCGTTGATACCACTG29340.1752805444569768No Hit
GGTATCAACGCAGAGTACTTTTTTT28550.17056099332810798No Hit
GGTATCAACGCAGAGTACATGGGAA25770.15395295264677208No Hit
GCGTTGATACCACTGCTTCCCATGT25090.14989055420673308No Hit
GTATCAACGCAGAGTACATGGGAAG24640.14720220229788372No Hit
ACGCAGAGTACATGGGAAGCAGTGG22840.13644879466248638No Hit
TATCAACGCAGAGTACATGGGAAGC22010.1314902789194976No Hit
TATCAACGCAGAGTACTTTTTTTTT20010.11954204821350055No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN19800.11828748398937086No Hit
GTGGTATCAACGCAGAGTACATGGG18840.11255233325049227No Hit
ACTCTGCGTTGATACCACTGCTTCC18200.1087288994245732No Hit
ATTCCATTCCATTCCATTCCATTCC16870.10078332600508516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGT701.091476E-412.2137086
TGCACGC550.003077969712.08599312
AAGATCG657.972848E-411.7012195
GTAGCAC803.7528438E-410.693083
GGTCCAA803.7872835E-410.68251511
TACACTG3400.010.3461145
CGAATAG856.618187E-410.05473415
CGCCCGC951.6457676E-49.99892810
GTACTAG1054.0450825E-59.9656411
CTACACC1151.0170348E-59.9205984
GTTCTAG1804.083631E-99.5126581
GTATTAG2600.09.5126581
GTGCTAA1502.5964073E-79.5126581
TAGGACT2303.6379788E-129.5072394
GTAGGAC1201.6896038E-59.5049613
CTTAGAC1651.0565236E-79.2169313
TTAGAAC1554.2262764E-79.1983483
GTCTTAC1451.6750328E-69.1846351
AGCACTC1451.6880458E-69.1794045
TAACACG1054.45782E-49.0545144
Produced by FastQC (version 0.11.5)