##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295016_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1673888
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.858256346900152	33.0	33.0	33.0	27.0	33.0
2	30.818867212143225	33.0	33.0	33.0	27.0	33.0
3	30.929860301286585	33.0	33.0	33.0	27.0	33.0
4	34.36227752394426	37.0	37.0	37.0	27.0	37.0
5	34.790816948326295	37.0	37.0	37.0	33.0	37.0
6	34.849423617350745	37.0	37.0	37.0	33.0	37.0
7	34.62426159934237	37.0	37.0	37.0	27.0	37.0
8	34.598984519872296	37.0	37.0	37.0	27.0	37.0
9	34.503172255252444	37.0	37.0	37.0	27.0	37.0
10	34.42673105966468	37.0	37.0	37.0	27.0	37.0
11	34.61181512741593	37.0	37.0	37.0	27.0	37.0
12	34.52241189374677	37.0	37.0	37.0	27.0	37.0
13	34.44483501883041	37.0	37.0	37.0	27.0	37.0
14	35.752173980576956	37.0	37.0	40.0	27.0	40.0
15	35.91859909384618	37.0	37.0	40.0	27.0	40.0
16	36.002053900858364	37.0	37.0	40.0	27.0	40.0
17	35.924438194192206	37.0	37.0	40.0	27.0	40.0
18	35.843462645051524	37.0	37.0	40.0	27.0	40.0
19	35.9386052113403	40.0	37.0	40.0	27.0	40.0
20	35.94011068840926	40.0	37.0	40.0	27.0	40.0
21	35.960594137720086	40.0	37.0	40.0	27.0	40.0
22	35.86102475195473	37.0	37.0	40.0	27.0	40.0
23	35.762346106788506	37.0	37.0	40.0	27.0	40.0
24	35.818957421285056	37.0	37.0	40.0	27.0	40.0
25	34.63206558622799	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5076032354157896
1101	2	0.4724290069392545
1101	3	0.4978116935918955
1101	4	0.6220524739561881
1101	5	0.5764003688132462
1101	6	0.5626701494654398
1101	7	0.4887658808987112
1101	8	0.5560289974424464
1101	9	0.6263013923212526
1101	10	0.8167274931718964
1101	11	0.6698183204568835
1101	12	0.6816283394268723
1101	13	0.6596549286493101
1101	14	1.1727373044706937
1101	15	1.1323012987811225
1101	16	1.0694452191812687
1101	17	1.0244396274185874
1101	18	1.0539019144715311
1101	19	0.9634707948406671
1101	20	0.917282145012166
1101	21	1.0064131734837716
1101	22	0.9451235564976912
1101	23	1.1338884824414848
1101	24	1.115829654390673
1101	25	1.1153607895587214
1102	1	0.3725079626404906
1102	2	0.34555609784356633
1102	3	0.37864872183710574
1102	4	0.4889485095007231
1102	5	0.3408166400005115
1102	6	0.43234264299437797
1102	7	0.25045828200745746
1102	8	0.4096915310666063
1102	9	0.5867571215595788
1102	10	0.691895117121895
1102	11	0.4835369763833839
1102	12	0.5832813118359539
1102	13	0.6461905902815985
1102	14	0.9902670548554653
1102	15	0.8880384252923363
1102	16	0.8176631923822271
1102	17	0.9091718296227569
1102	18	0.9429863773747442
1102	19	0.8281624187490877
1102	20	0.8354538347410738
1102	21	0.6836928133422262
1102	22	0.7528585036682429
1102	23	0.9893295268128384
1102	24	0.8395850790471826
1102	25	1.0029593383771171
1103	1	0.2588002896918873
1103	2	0.2582372552729346
1103	3	0.3582661642364151
1103	4	0.373040113784576
1103	5	0.30188103396072563
1103	6	0.17024782600329758
1103	7	0.17855701505786215
1103	8	0.3228937918063082
1103	9	0.2669456101523906
1103	10	0.47790848447500167
1103	11	0.38466014176088237
1103	12	0.5090239374761865
1103	13	0.3924367842736771
1103	14	0.7073903814178166
1103	15	0.561242847374416
1103	16	0.6717960880742524
1103	17	0.6369733475689188
1103	18	0.6242961386440982
1103	19	0.5296321926201628
1103	20	0.5794379458218657
1103	21	0.7401276607752649
1103	22	0.6729431745611336
1103	23	0.6946646844656641
1103	24	0.7886036525489999
1103	25	0.7588683812748798
1104	1	0.0542809712920409
1104	2	0.0827527963320307
1104	3	0.09014931849581842
1104	4	-5.158028501170975E-4
1104	5	-0.010446287604892746
1104	6	-0.07430171112256545
1104	7	0.03768978558741054
1104	8	0.20516127549778673
1104	9	0.17436868430818464
1104	10	0.37809645214576193
1104	11	0.304499275011068
1104	12	0.22673546855831006
1104	13	0.24984636792678572
1104	14	0.5160138105237237
1104	15	0.3916264573130164
1104	16	0.22916130296297155
1104	17	0.3885958053586336
1104	18	0.3942545878958228
1104	19	0.2949651981842507
1104	20	0.326239497529464
1104	21	0.2917141438911557
1104	22	0.259448474829739
1104	23	0.34755285119629065
1104	24	0.29644882954439566
1104	25	0.3226661676332796
1105	1	0.1717792163540075
1105	2	0.18231224637075982
1105	3	0.30194734328280504
1105	4	0.30974846661154487
1105	5	0.18096540875451694
1105	6	0.021331216356131222
1105	7	0.13478506056804918
1105	8	0.31813268816238605
1105	9	0.35728738831670626
1105	10	0.4994585866345318
1105	11	0.31606582952307605
1105	12	0.4786985785505635
1105	13	0.37764579666420417
1105	14	0.6334152314183825
1105	15	0.5315621902719627
1105	16	0.5432516399110767
1105	17	0.3856656639953542
1105	18	0.3748791642900926
1105	19	0.4971334543809931
1105	20	0.41112796819173525
1105	21	0.47376415357356905
1105	22	0.49257141731618503
1105	23	0.5897700035975291
1105	24	0.6021793073716637
1105	25	0.40703239294312255
1106	1	0.06166548362508806
1106	2	-0.07252779393078868
1106	3	-0.025536269612757678
1106	4	-0.004911638427451237
1106	5	-0.16157188002195966
1106	6	-0.20290788168482266
1106	7	-0.720884104977003
1106	8	-0.20241055507093364
1106	9	0.126862389876905
1106	10	-0.3747399128370006
1106	11	-0.18764160580538913
1106	12	-0.6162188076157591
1106	13	-0.12275501617489226
1106	14	-0.09072274564817917
1106	15	0.013614218515087373
1106	16	1.7460541881320069E-4
1106	17	-0.23513251756251918
1106	18	-0.40599735765566436
1106	19	-0.5928697194159227
1106	20	-0.06359062014610828
1106	21	0.14979457797129214
1106	22	0.22990201048247627
1106	23	-0.5170768208200869
1106	24	0.15266937699161787
1106	25	0.3179027641972638
1107	1	-1.3331713627181614
1107	2	-1.2390619939966427
1107	3	-1.097815830729722
1107	4	-1.1656958710877419
1107	5	-1.0525080556441821
1107	6	-0.9264086069008073
1107	7	-1.1272327682471186
1107	8	-1.0367898129986202
1107	9	-1.0100466030454243
1107	10	-1.0067807378334592
1107	11	-0.9129640384097826
1107	12	-0.9651716432664017
1107	13	-1.10731445092474
1107	14	-1.284972713135133
1107	15	-1.0890837216631795
1107	16	-1.0288088047060882
1107	17	-1.096090277943219
1107	18	-1.2078915622269548
1107	19	-1.2142070368931996
1107	20	-1.3239333101301511
1107	21	-1.356384529134914
1107	22	-1.1988690190312354
1107	23	-1.197314274323169
1107	24	-0.9088013652515983
1107	25	-1.0557635700414423
1108	1	-0.058502184760982345
1108	2	-0.29216609502874036
1108	3	-0.13708696255720554
1108	4	-0.2612428548986898
1108	5	-0.49708421863937957
1108	6	-0.11447061986375928
1108	7	-0.06318155293687511
1108	8	0.14109732712923062
1108	9	-0.2971052624382011
1108	10	-0.5825515189795567
1108	11	-0.2369864904227441
1108	12	0.025281306526423464
1108	13	0.0983649765660175
1108	14	-0.36665254908880485
1108	15	-0.014615721496767264
1108	16	-0.8246959921797767
1108	17	-0.8000901188163709
1108	18	-0.7463856199740349
1108	19	-0.09181496264259437
1108	20	-1.0572347620645317
1108	21	-0.31934733645738334
1108	22	-0.3535135547401538
1108	23	-0.23945557251524008
1108	24	-0.4552068005538601
1108	25	-1.2571666037330473
1109	1	0.23231956247120067
1109	2	0.09679530090241428
1109	3	0.23088286511373113
1109	4	0.15311408174768815
1109	5	0.12028954420497939
1109	6	0.05373116707669823
1109	7	0.15112671428942548
1109	8	0.24549919825830102
1109	9	0.28297457439045104
1109	10	0.3845528335443049
1109	11	0.2598316276161867
1109	12	0.2791292071323497
1109	13	0.3769058971772452
1109	14	0.4660359483208012
1109	15	0.45261965886695066
1109	16	0.24344946566863968
1109	17	0.2956427660533336
1109	18	0.4081521256014895
1109	19	0.30564704233415796
1109	20	0.3140715644855874
1109	21	0.32487513566446324
1109	22	0.45525371934527925
1109	23	0.5868053077776381
1109	24	0.46328141066871353
1109	25	0.5342313812987314
1110	1	-0.12188296935903153
1110	2	-0.026616536275877678
1110	3	0.0077295687678535785
1110	4	-1.204621686736587
1110	5	-0.35364995100714225
1110	6	-0.1631322731011693
1110	7	-0.12409838996572375
1110	8	0.09417701951301893
1110	9	0.094329947683228
1110	10	-0.06404528366308426
1110	11	-0.11563172675715805
1110	12	0.05584886173929959
1110	13	-0.013669795475458102
1110	14	0.05516543026438825
1110	15	0.03367595631555531
1110	16	0.014093974344909554
1110	17	0.13332116353781487
1110	18	0.08449122985265944
1110	19	0.00632293530793504
1110	20	-0.052468252062489285
1110	21	0.0943069757736339
1110	22	-0.45239382431788044
1110	23	-0.007992975336172492
1110	24	0.07689250133438463
1110	25	0.3152176558904429
1111	1	-0.18563120304277092
1111	2	-0.4273405148283551
1111	3	-0.21987008283456078
1111	4	-0.34841370334901
1111	5	-0.3139662432589958
1111	6	-0.22630507171015068
1111	7	-0.11600570591295423
1111	8	-0.1968568093137435
1111	9	-0.30958566482337346
1111	10	-0.22466968098432005
1111	11	-0.46291543997414664
1111	12	-0.17830656765750774
1111	13	-0.32468823111238265
1111	14	-0.27480375922867495
1111	15	-0.31434926792718443
1111	16	-0.45092230753222395
1111	17	-0.25204420757558665
1111	18	-0.2891760407996671
1111	19	-0.4224007247647563
1111	20	-0.3158928588083043
1111	21	-0.43018741745400746
1111	22	-0.38150392262036803
1111	23	-0.07455484928412659
1111	24	-0.00483737472389123
1111	25	-0.011579201453933763
1112	1	-0.06728091676279035
1112	2	-0.34606341030728416
1112	3	-0.1100581600569619
1112	4	-0.12563221813358894
1112	5	-0.04224878667240972
1112	6	-0.11530014337732553
1112	7	0.14636192284962846
1112	8	0.03836046656455494
1112	9	0.09235768677927325
1112	10	0.21918696757843747
1112	11	-0.09152584970918554
1112	12	-0.059117524727781756
1112	13	-0.10771436034624315
1112	14	-0.23082782389440126
1112	15	-0.20433499596934013
1112	16	-0.20038107861106624
1112	17	-0.37235568195953306
1112	18	-0.17928155183748373
1112	19	-0.20391902178964472
1112	20	-0.806919334296353
1112	21	-1.3083260696485723
1112	22	-1.6107197809516833
1112	23	-0.4638138846298929
1112	24	-0.19565168682076717
1112	25	-1.483158239071308
1113	1	-0.15125580084242074
1113	2	-0.08984547699640544
1113	3	-0.18612272411990816
1113	4	-0.5035119339118381
1113	5	-0.349728065709229
1113	6	-0.1796433142636431
1113	7	-0.17807068465505438
1113	8	-0.08376173607458526
1113	9	-0.21221258635677032
1113	10	-0.012394512677893488
1113	11	-0.09089674583756846
1113	12	-0.16231440285977072
1113	13	-0.22760242891376947
1113	14	-0.4583510292080888
1113	15	-0.5280035252365778
1113	16	-0.5720717058540714
1113	17	-0.47707167259874694
1113	18	-0.8629164375450316
1113	19	-0.9783006679068649
1113	20	-1.3933936421656128
1113	21	-1.0098253950508038
1113	22	-0.4858324537154459
1113	23	-2.1796737974076805
1113	24	-1.0599707952719157
1113	25	-0.5876430604159495
1114	1	-0.30378500545973
1114	2	-0.46568878026133476
1114	3	-0.4304502901512208
1114	4	-0.6520831074065825
1114	5	-0.8489805775788071
1114	6	-0.6190162622314048
1114	7	-0.359608015381653
1114	8	-0.23269426168074148
1114	9	-1.415720098071617
1114	10	-0.3526677052806235
1114	11	-0.42698556588531034
1114	12	-0.3893158266336414
1114	13	-0.4866373955842036
1114	14	-0.9136604405478366
1114	15	-1.4929730465073305
1114	16	-1.240484382836506
1114	17	-1.6511760218869824
1114	18	-1.7858078131011936
1114	19	-1.7478835834967086
1114	20	-1.0336292070456423
1114	21	-0.8977187437346359
1114	22	-0.538613687395376
1114	23	-0.3872627120724417
1114	24	-1.1759917977850805
1114	25	-0.610371272964926
1115	1	-0.752763581726537
1115	2	-0.6187758414388682
1115	3	-0.5825093502348331
1115	4	-0.7991286608222552
1115	5	-0.5647419532395688
1115	6	-0.5579039266798418
1115	7	-0.4191987593042299
1115	8	-0.35284230422357155
1115	9	-0.6967770684007917
1115	10	-0.408376796693247
1115	11	-0.4687072362118414
1115	12	-0.5263314819060909
1115	13	-0.4232912094725947
1115	14	-0.4527954483772021
1115	15	-1.452914467609581
1115	16	-1.0306974431670568
1115	17	-0.6435878246723234
1115	18	-2.554849272398293
1115	19	-1.1692206570789097
1115	20	-0.9502105314953084
1115	21	-1.0559977369138025
1115	22	-0.8103488355417738
1115	23	-0.6024592424569661
1115	24	-0.4662603906568421
1115	25	-0.6522141051995334
1116	1	-1.502245452210417
1116	2	-0.8494247852504451
1116	3	-1.6899048835605086
1116	4	-0.9692453723069789
1116	5	-1.1904697565365012
1116	6	-0.5701874886706264
1116	7	-1.218926217988809
1116	8	-3.2771319045424434
1116	9	-1.7389616339193523
1116	10	-0.9902402222507476
1116	11	-0.7774762712003707
1116	12	-0.9778954545529999
1116	13	-1.983703727066505
1116	14	-1.8263306728803883
1116	15	-1.5993607046226117
1116	16	-1.0964156416056454
1116	17	-2.022939593613202
1116	18	-2.7309977380429373
1116	19	-1.4545064016145872
1116	20	-1.094673171502727
1116	21	-0.9557999654070173
1116	22	-0.6197783775395038
1116	23	-0.6175325729208367
1116	24	-0.6355001744229654
1116	25	-0.6465956436653286
1201	1	0.2800155428329205
1201	2	0.26809084664649774
1201	3	0.36416661409329976
1201	4	0.4793363001373052
1201	5	0.40787132245799995
1201	6	0.17075282897177146
1201	7	0.2567935591723156
1201	8	0.39760919216445245
1201	9	0.5071885545912025
1201	10	0.6543665975860193
1201	11	0.35939068617459213
1201	12	0.5148447452878671
1201	13	0.47662506289534434
1201	14	0.9775974386683828
1201	15	0.8259277544828336
1201	16	0.8151086300069963
1201	17	0.8476268588177547
1201	18	0.7891666358517995
1201	19	0.7417566683100318
1201	20	0.8027454920263182
1201	21	0.8091374929477553
1201	22	0.8118217059618189
1201	23	0.7759677489950292
1201	24	0.7649605237267849
1201	25	1.0232926416898849
1202	1	0.3091139741004767
1202	2	0.4394117439079075
1202	3	0.4291890307543902
1202	4	0.4434166530525232
1202	5	0.29879198594654355
1202	6	0.22047635475247773
1202	7	0.21031493339978624
1202	8	0.3092584498178539
1202	9	0.3972193999664384
1202	10	0.7527298466569974
1202	11	0.2292333667040154
1202	12	0.3206172679276378
1202	13	0.38887068306277683
1202	14	0.7107857175497401
1202	15	0.475579884096625
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2210	17	0.33572150709713355
2210	18	0.43991498607582713
2210	19	0.3707724804189425
2210	20	0.4493271186359422
2210	21	0.3893842588857197
2210	22	0.37349824167146295
2210	23	0.4845641808775625
2210	24	0.4801539239395467
2210	25	0.508329338812203
2211	1	0.22601793087850552
2211	2	0.06935993918917305
2211	3	0.08013991999957781
2211	4	0.10403365673634823
2211	5	0.186452208635707
2211	6	0.14238355673457193
2211	7	0.12453189085056948
2211	8	0.10528463076094852
2211	9	0.14172359502246934
2211	10	0.2904290414314801
2211	11	0.128738584221594
2211	12	0.271213852016956
2211	13	0.1535000333403218
2211	14	-0.027474793799584063
2211	15	0.0764420118242981
2211	16	0.03637464882763197
2211	17	0.07023349332855844
2211	18	0.02551339898604965
2211	19	0.03491468126695452
2211	20	0.06141043267983548
2211	21	0.2270407416355127
2211	22	0.16058216843876494
2211	23	0.14424575533162454
2211	24	0.2810969733413913
2211	25	0.15905303323478392
2212	1	0.31422807327873414
2212	2	0.2865351587915761
2212	3	0.21586623687050377
2212	4	0.35714850967235634
2212	5	0.18929750959036085
2212	6	0.24589462675049845
2212	7	0.22479873920121918
2212	8	0.27424978907204434
2212	9	0.09212940999139363
2212	10	0.2891961858296881
2212	11	0.2486679924238686
2212	12	0.23858035349178408
2212	13	0.12474692078546923
2212	14	0.20822448676428706
2212	15	0.21465296531373923
2212	16	0.09021285815894231
2212	17	0.11712702164773958
2212	18	0.11539735915616234
2212	19	0.22404000266105584
2212	20	0.053515966200805565
2212	21	0.07138366474696056
2212	22	-0.40358837117889124
2212	23	-0.10950255827872013
2212	24	0.10833037507658361
2212	25	0.2106107300658593
2213	1	-0.009334739078198595
2213	2	-0.8206215746241163
2213	3	-0.21840118473926395
2213	4	0.059622755291506735
2213	5	0.01210044136706756
2213	6	0.021920375056438957
2213	7	0.07280840528547117
2213	8	0.19737854275156508
2213	9	-0.03389004367528514
2213	10	-1.4231377655932747
2213	11	-0.33431525814852847
2213	12	0.006100583583467767
2213	13	0.045466650097885974
2213	14	0.20859867527929765
2213	15	0.04135068898088434
2213	16	0.14877174509094004
2213	17	0.11388224497042643
2213	18	0.1405772087621031
2213	19	0.28437198736469327
2213	20	0.1760926270226335
2213	21	0.13391063023191663
2213	22	0.23750551562773126
2213	23	-0.5565409886671233
2213	24	-1.0674173750745837
2213	25	-1.212052840724013
2214	1	-0.045461501417335626
2214	2	-0.24046212850690907
2214	3	0.05240325550369107
2214	4	-0.04453954792885639
2214	5	-0.0488947485927298
2214	6	-0.09599971353846115
2214	7	0.05232829668261729
2214	8	0.04729005592012214
2214	9	-0.38482819963956416
2214	10	-0.27102918538108156
2214	11	-0.24996201480639257
2214	12	-0.3057515572112024
2214	13	-0.10476283930299246
2214	14	-0.14018893419710565
2214	15	-0.22580878338132493
2214	16	-0.12995868629973018
2214	17	-0.21120175190693402
2214	18	-0.00820520317432738
2214	19	-0.7262225031400149
2214	20	-0.3881062545285232
2214	21	-0.35261410414034344
2214	22	-0.7053773796799234
2214	23	-1.8208811815524584
2214	24	-1.3823973027927536
2214	25	-1.4808891629859033
2215	1	-0.24956144171750694
2215	2	-0.034294118835063614
2215	3	-1.1900632228189103
2215	4	-0.8791476255181294
2215	5	-0.3094868678472764
2215	6	-0.23901563533299708
2215	7	-0.07613687618269438
2215	8	0.041652220686920316
2215	9	-0.22443505352692483
2215	10	-1.0253990893066742
2215	11	-0.6111023420014163
2215	12	-0.3692030643098505
2215	13	-0.3686987798881205
2215	14	-0.6868829543036696
2215	15	-0.8351589700648745
2215	16	-0.5663526063282518
2215	17	-0.42508223815954693
2215	18	-0.03697862799657514
2215	19	-0.9004284967294538
2215	20	-0.9797119748814822
2215	21	-0.4938388996270291
2215	22	-0.27371147054300593
2215	23	-0.4031738863174823
2215	24	-1.4374081583825173
2215	25	-0.4793256899227245
2216	1	-0.31822197924162055
2216	2	-0.1498518834709266
2216	3	-1.1086294171363669
2216	4	-0.35457406947573133
2216	5	-0.29018766691523723
2216	6	-0.21336356970221004
2216	7	-0.20590627352589053
2216	8	-0.3274523841902237
2216	9	-0.126995616800194
2216	10	-0.23912760881482598
2216	11	-0.27617023956199915
2216	12	-0.23811982700819811
2216	13	-0.9103881353572802
2216	14	-0.3673676293366981
2216	15	-0.7353504002403497
2216	16	-0.5855811026199476
2216	17	-1.5506462923425133
2216	18	-0.5809769648545853
2216	19	-0.5255641018978565
2216	20	-0.3052087013621616
2216	21	-0.19001050717616863
2216	22	-0.12259560357748
2216	23	-0.09964414182505976
2216	24	-0.3005640758332717
2216	25	-0.16325273117624306
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2190.0
3	241.0
4	278.0
5	243.0
6	166.0
7	117.0
8	219.0
9	466.0
10	940.0
11	1801.0
12	2940.0
13	4544.0
14	6002.0
15	7636.0
16	8641.0
17	9193.0
18	9648.0
19	9671.0
20	10154.0
21	10989.0
22	11715.0
23	12373.0
24	13231.0
25	14284.0
26	14904.0
27	16749.0
28	18789.0
29	22071.0
30	27041.0
31	34177.0
32	45405.0
33	62366.0
34	90841.0
35	142115.0
36	254432.0
37	807316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.26279568964588	22.061141253866722	14.420881558746013	22.25518149774139
2	14.833326849355243	21.62079501551666	40.603189518699295	22.942688616428804
3	17.94463966094187	27.261794302338803	30.323553884741784	24.470012151977542
4	12.079892371977676	18.995603512639526	38.90541055935318	30.01909355602962
5	12.339541463192605	39.15799169454516	35.35471862187423	13.147748220388012
6	29.328202264206958	39.56112957745299	18.00890335069828	13.101764807641771
7	24.777624882310725	33.819572841056484	23.888004702184038	17.514797574448753
8	23.239472456892706	37.82761153774541	21.735259763313255	17.197656242048627
9	23.414729937574048	18.120707729574164	22.9212254811863	35.54333685166548
10	14.514369471265248	31.475003861956317	33.92995069868478	20.080675968093654
11	30.86042559457714	26.763468711334003	23.3370666190977	19.039039074991155
12	20.189278224862626	29.101601902719686	31.69742782039102	19.011692052026667
13	25.886184702888787	24.832450232001197	26.790839694656487	22.490525370453526
14	19.69019052396785	25.03864237455147	28.639357393175235	26.631809708305447
15	22.926801528796624	31.15243371373672	24.350665481460197	21.570099276006452
16	20.49334591200992	32.245449408339276	26.90559767071004	20.35560700894076
17	19.55020936155948	31.234410121187274	28.996231791231057	20.21914872602219
18	19.556394164114526	30.64079325132955	29.957401771454155	19.84541081310177
19	20.263501787787018	29.92904523852425	30.294444600751515	19.51300837293722
20	21.478704408170447	29.322255990067987	29.421085302686468	19.7779542990751
21	21.504536391394474	29.780121046258202	28.754142970104766	19.96119959224255
22	20.43172892102489	29.62492943894156	29.48168225943842	20.461659380595126
23	20.055528550870044	30.132320298450992	29.277362597366523	20.53478855331244
24	20.32716491401174	29.69696352833266	29.410475695533783	20.565395862121818
25	20.910362862741504	29.132773075032585	28.404341492940492	21.552522569285415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3585.0
1	3585.0
2	3026.0
3	2467.0
4	2467.0
5	2467.0
6	3472.0
7	4477.0
8	4477.0
9	4477.0
10	6538.0
11	8599.0
12	8599.0
13	8599.0
14	13962.5
15	19326.0
16	19326.0
17	19326.0
18	30302.0
19	41278.0
20	41278.0
21	41278.0
22	57886.5
23	74495.0
24	74495.0
25	74495.0
26	94657.5
27	114820.0
28	114820.0
29	114820.0
30	134861.5
31	154903.0
32	154903.0
33	154903.0
34	169274.5
35	183646.0
36	183646.0
37	183646.0
38	190981.0
39	198316.0
40	198316.0
41	198316.0
42	194269.0
43	190222.0
44	190222.0
45	190222.0
46	197906.0
47	205590.0
48	205590.0
49	205590.0
50	186857.5
51	168125.0
52	168125.0
53	168125.0
54	145013.0
55	121901.0
56	121901.0
57	121901.0
58	103430.5
59	84960.0
60	84960.0
61	84960.0
62	68833.0
63	52706.0
64	52706.0
65	52706.0
66	39646.5
67	26587.0
68	26587.0
69	26587.0
70	18969.5
71	11352.0
72	11352.0
73	11352.0
74	7800.0
75	4248.0
76	4248.0
77	4248.0
78	2872.0
79	1496.0
80	1496.0
81	1496.0
82	1008.5
83	521.0
84	521.0
85	521.0
86	349.0
87	177.0
88	177.0
89	177.0
90	120.0
91	63.0
92	63.0
93	63.0
94	41.5
95	20.0
96	20.0
97	20.0
98	14.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30838383452178403
2	0.18537679940354432
3	0.2018056166242903
4	0.22050459767917566
5	0.24744785792119905
6	0.1960107247318817
7	0.18967816245770325
8	0.2138135884838173
9	0.21441100001911717
10	0.2240293257374448
11	0.19899778240838098
12	0.20533034468255942
13	0.21733831654208646
14	0.20562905045020935
15	0.19672761857424154
16	0.1992964881760309
17	0.19911726471544094
18	0.20288095738783002
19	0.20234328700606016
20	0.19899778240838098
21	0.1963691716530616
22	0.20007312317192072
23	0.20288095738783002
24	0.21865262191974613
25	0.2142915177120572
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1673888.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.46152156428239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.55207978337117	79.0155100400667
2	5.264974573106067	8.893755268835932
3	0.5802232210217348	1.4701960828327394
4	0.18695176985489836	0.6316092376432103
5	0.09548546786893504	0.40324239517438254
6	0.060309955342571296	0.3056322356224496
7	0.04160718004247319	0.24599440140704304
8	0.033791635805792926	0.22832743810426848
9	0.022207958798681603	0.16881461918761867
>10	0.13846239034515379	2.1581736068525417
>50	0.013175206059594853	0.7668911162859559
>100	0.007604489196937836	1.2273702717856583
>500	0.001705292283251919	1.04266861979881
>1k	0.0012789692124389393	2.4660607185894268
>5k	1.4210769027099326E-4	0.9757539478133335
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8654	0.5169999426484926	No Hit
CCCATGTACTCTGCGTTGATACCAC	7605	0.4543314725955381	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4156	0.24828423407061884	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3211	0.19182884398478275	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3161	0.18884178630828347	No Hit
CATGTACTCTGCGTTGATACCACTG	2934	0.1752805444569768	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2855	0.17056099332810798	No Hit
GGTATCAACGCAGAGTACATGGGAA	2577	0.15395295264677208	No Hit
GCGTTGATACCACTGCTTCCCATGT	2509	0.14989055420673308	No Hit
GTATCAACGCAGAGTACATGGGAAG	2464	0.14720220229788372	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2284	0.13644879466248638	No Hit
TATCAACGCAGAGTACATGGGAAGC	2201	0.1314902789194976	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2001	0.11954204821350055	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	1980	0.11828748398937086	No Hit
GTGGTATCAACGCAGAGTACATGGG	1884	0.11255233325049227	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1820	0.1087288994245732	No Hit
ATTCCATTCCATTCCATTCCATTCC	1687	0.10078332600508516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.974115352998528E-5	0.0
11	0.0	0.0	0.0	5.974115352998528E-5	0.0
12	0.0	0.0	0.0	5.974115352998528E-5	0.0
13	0.0	0.0	0.0	5.974115352998528E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGT	70	1.091476E-4	12.213708	6
TGCACGC	55	0.0030779697	12.085993	12
AAGATCG	65	7.972848E-4	11.701219	5
GTAGCAC	80	3.7528438E-4	10.69308	3
GGTCCAA	80	3.7872835E-4	10.682515	11
TACACTG	340	0.0	10.346114	5
CGAATAG	85	6.618187E-4	10.054734	15
CGCCCGC	95	1.6457676E-4	9.998928	10
GTACTAG	105	4.0450825E-5	9.965641	1
CTACACC	115	1.0170348E-5	9.920598	4
GTTCTAG	180	4.083631E-9	9.512658	1
GTATTAG	260	0.0	9.512658	1
GTGCTAA	150	2.5964073E-7	9.512658	1
TAGGACT	230	3.6379788E-12	9.507239	4
GTAGGAC	120	1.6896038E-5	9.504961	3
CTTAGAC	165	1.0565236E-7	9.216931	3
TTAGAAC	155	4.2262764E-7	9.198348	3
GTCTTAC	145	1.6750328E-6	9.184635	1
AGCACTC	145	1.6880458E-6	9.179404	5
TAACACG	105	4.45782E-4	9.054514	4
>>END_MODULE