FastQCFastQC Report
Wed 25 May 2016
SRR1295016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673888
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC85150.5086959223078247No Hit
GTACATGGGAAGCAGTGGTATCAAC84350.5039166300254259No Hit
GTATCAACGCAGAGTACTTTTTTTT78320.4678927144468447No Hit
GGTATCAACGCAGAGTACTTTTTTT73070.43652860884360245No Hit
TATCAACGCAGAGTACTTTTTTTTT52430.31322286795771287No Hit
GAGTACATGGGAAGCAGTGGTATCA46100.2754067177732321No Hit
GCTTCCCATGTACTCTGCGTTGATA35010.20915377850847847No Hit
CATGTACTCTGCGTTGATACCACTG34070.20353811007665984No Hit
ACGCAGAGTACATGGGAAGCAGTGG29310.17510132099638687No Hit
GCGTTGATACCACTGCTTCCCATGT27600.16488558374275936No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27160.16225697298744002No Hit
GTATCAACGCAGAGTACATGGGAAG26730.15968810338565065No Hit
GGTATCAACGCAGAGTACATGGGAA26430.1578958687797511No Hit
TATCAACGCAGAGTACATGGGAAGC23770.142004721940775No Hit
ACTCTGCGTTGATACCACTGCTTCC20710.12372392896059953No Hit
GTGGTATCAACGCAGAGTACATGGG19320.11541990861993155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGGA400.00528263414.24865512
CCGGAAT550.003070783312.08976914
CATCCGG550.003070783312.08976911
TCCGGAA658.03034E-411.69120413
GTCCTAC803.7132623E-410.70535851
TAGCCTA1252.5725403E-69.8790685
CCCCTAC1304.1483363E-69.5158751
TCTAGGA2054.2382453E-109.2676952
ACTGCCC1651.0665826E-79.2112528
TAAGTAG1451.7078E-69.1715485
GTCTAGG1356.701661E-69.1634351
TAGACAG2700.09.1472855
TAAGACT2501.8189894E-129.119144
TTAGCCT1151.1115635E-49.08614
TTAGAAC1901.0586518E-88.9991513
GTGTTAC1401.0620632E-58.8361691
TAGGATA1401.0860043E-58.8205974
GAATAGG2054.0145096E-98.8195911
GTATAGA2605.456968E-128.7838841
GGCATAA1304.282673E-58.7838841
Produced by FastQC (version 0.11.5)