FastQCFastQC Report
Wed 25 May 2016
SRR1295015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352300
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1042667.710271389484581No Hit
CCCATGTACTCTGCGTTGATACCAC1025677.58463358722177No Hit
GAGTACATGGGAAGCAGTGGTATCA514703.806108112105302No Hit
CATGTACTCTGCGTTGATACCACTG421863.1195740590105747No Hit
GCGTTGATACCACTGCTTCCCATGT321412.3767655106115506No Hit
ACGCAGAGTACATGGGAAGCAGTGG311822.3058492937957555No Hit
TATCAACGCAGAGTACATGGGAAGC286292.117059824003549No Hit
GGTATCAACGCAGAGTACATGGGAA283152.0938401242327886No Hit
ACTCTGCGTTGATACCACTGCTTCC273452.022110478444132No Hit
GTATCAACGCAGAGTACATGGGAAG240661.7796346964430971No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA184321.3630111661613546No Hit
GCTTCCCATGTACTCTGCGTTGATA180751.3366116985875915No Hit
GTGGTATCAACGCAGAGTACATGGG145601.0766841677142647No Hit
GTACTCTGCGTTGATACCACTGCTT141601.0471049323374992No Hit
ATACCACTGCTTCCCATGTACTCTG126300.9339643570213709No Hit
ACATGGGAAGCAGTGGTATCAACGC119750.8855283590919175No Hit
GATACCACTGCTTCCCATGTACTCT119160.8811654218738446No Hit
CAGTGGTATCAACGCAGAGTACATG115550.8544701619463138No Hit
GTTGATACCACTGCTTCCCATGTAC114880.8495156400207056No Hit
GGGAAGCAGTGGTATCAACGCAGAG111720.8261480440730606No Hit
CATGGGAAGCAGTGGTATCAACGCA110270.8154255712489832No Hit
CTGCTTCCCATGTACTCTGCGTTGA108260.8005620054721585No Hit
TACCACTGCTTCCCATGTACTCTGC106310.7861421282259853No Hit
GCAGTGGTATCAACGCAGAGTACAT100740.7449530429638394No Hit
GCAGAGTACATGGGAAGCAGTGGTA99760.7377061302965319No Hit
CTTCCCATGTACTCTGCGTTGATAC96630.7145603786142128No Hit
CCACTGCTTCCCATGTACTCTGCGT93200.6891961842786365No Hit
AAGCAGTGGTATCAACGCAGAGTAC83810.6197589292316794No Hit
CCATGTACTCTGCGTTGATACCACT83580.6180581231975154No Hit
TTCCCATGTACTCTGCGTTGATACC76180.5633365377504991No Hit
ATGGGAAGCAGTGGTATCAACGCAG76110.5628189011314058No Hit
TGATACCACTGCTTCCCATGTACTC67780.5012201434592916No Hit
GTACATGGTAAGCAGTGGTATCAAC66770.49375138652665823No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA65020.48081047104932334No Hit
ACCATGTACTCTGCGTTGATACCAC54720.4046439399541522No Hit
ATCAACGCAGAGTACATGGGAAGCA48460.35835243658951416No Hit
GAACAAAAAAAAAAAAAAAAAAAAA40300.29801079642091255No Hit
CCCCATGTACTCTGCGTTGATACCA35690.2639207276491902No Hit
GGAAGCAGTGGTATCAACGCAGAGT34110.25223692967536787No Hit
GTATCAACGCAGAGTACTTTTTTTT33790.24987059084522664No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33210.24558160171559562No Hit
GTACATGGGTAAGCAGTGGTATCAA33170.24528580936182798No Hit
AAAAAGTACTCTGCGTTGATACCAC32470.24010944317089403No Hit
GGTAAGCAGTGGTATCAACGCAGAG32020.2367817791910079No Hit
GGTATCAACGCAGAGTACTTTTTTT31740.23471123271463432No Hit
TCCATGTACTCTGCGTTGATACCAC30390.22472824077497594No Hit
CTGCGTTGATACCACTGCTTCCCAT28880.213562079420247No Hit
TATCAACGCAGAGTACTTTTTTTTT28530.21097389632478No Hit
CTCTGCGTTGATACCACTGCTTCCC27670.20461436071877542No Hit
TCAACGCAGAGTACATGGGAAGCAG27350.2022480218886342No Hit
GAGTACATGGTAAGCAGTGGTATCA25910.1915994971529986No Hit
AAACAAAAAAAAAAAAAAAAAAAAA25800.19078606818013755No Hit
TGGGAAGCAGTGGTATCAACGCAGA24220.17910227020631517No Hit
AGTGGTATCAACGCAGAGTACATGG24090.1781409450565703No Hit
AAAGTACTCTGCGTTGATACCACTG23760.17570065813798713No Hit
TCCCATGTACTCTGCGTTGATACCA23000.1700806034164017No Hit
GTACATGGAAAGCAGTGGTATCAAC22700.16786216076314428No Hit
GCTTACCATGTACTCTGCGTTGATA22550.16675293943651556No Hit
ACTCTGCGTTGATACCACTGCTTAC21610.15980181912297567No Hit
AACGCAGAGTACATGGGAAGCAGTG20130.14885750203357243No Hit
ACTGCTTCCCATGTACTCTGCGTTG19160.1416845374547068No Hit
TTGATACCACTGCTTCCCATGTACT17910.1324410263994676No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17800.1316275974266065No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17700.1308881165421874No Hit
ATGTACTCTGCGTTGATACCACTGC17540.12970494712711678No Hit
ACGCAGAGTACATGGTAAGCAGTGG17420.1288175700658138No Hit
GAGTACATGGAAAGCAGTGGTATCA17130.1266730755009983No Hit
GCGTTGATACCACTGCTTACCATGT16520.12216224210604155No Hit
GGTATCAACGCAGAGTACATGGTAA15900.11757746062264289No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN15740.11639429120757229No Hit
TATCAACGCAGAGTACATGGTAAGC15150.11203135398949937No Hit
CAACGCAGAGTACATGGGAAGCAGT14640.10826000147896177No Hit
CTGCTTACCATGTACTCTGCGTTGA14500.10722472824077496No Hit
AAAAACAAAAAAAAAAAAAAAAAAA14290.10567181838349478No Hit
ATGGTAAGCAGTGGTATCAACGCAG13800.102048362049841No Hit
CGTTGATACCACTGCTTCCCATGTA13530.10005176366190933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA5150.017.1749821
TACATAA3800.016.5072482
GTACATA5500.014.8715531
TACAAGA1101.2732926E-1114.6882682
GTACAAG1400.013.58696651
TACAAAA6400.013.3652432
GGTCAGT655.465035E-513.15035617
GTCAGTC655.465035E-513.15035618
AGAACAA8450.013.047152
CTCGGCG604.0954456E-412.6651847
AGTGGTT905.423044E-712.66283614
ACATAAA8400.012.5590873
ACATAGA2650.012.1940343
AGTACAA802.8528508E-511.8802162
TCAGTCA802.8734963E-511.87184919
TACAAAG1052.703182E-711.7670722
GTAAATA657.945569E-411.7056941
ACTCGGC658.030098E-411.6909396
GTAAAAA2050.011.598631
TACATAG2250.011.4050082
Produced by FastQC (version 0.11.5)