FastQCFastQC Report
Wed 25 May 2016
SRR1295015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352300
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1153858.532500184870221No Hit
GTACATGGGAAGCAGTGGTATCAAC1046647.739702728684464No Hit
GAGTACATGGGAAGCAGTGGTATCA562104.156622051319974No Hit
CATGTACTCTGCGTTGATACCACTG479553.546180581231975No Hit
ACGCAGAGTACATGGGAAGCAGTGG390192.8853804629150335No Hit
GCGTTGATACCACTGCTTCCCATGT350262.590105745766472No Hit
TATCAACGCAGAGTACATGGGAAGC324632.400576795089847No Hit
GGTATCAACGCAGAGTACATGGGAA313192.3159801819122974No Hit
ACTCTGCGTTGATACCACTGCTTCC311112.3005989795163795No Hit
GTATCAACGCAGAGTACATGGGAAG256371.8958071433853436No Hit
GCTTCCCATGTACTCTGCGTTGATA201841.4925682171115875No Hit
GTGGTATCAACGCAGAGTACATGGG161861.1969237595208164No Hit
ACATGGGAAGCAGTGGTATCAACGC154751.1443466686386157No Hit
CATGGGAAGCAGTGGTATCAACGCA143451.060785328699253No Hit
GTACTCTGCGTTGATACCACTGCTT139301.030096871995859No Hit
CAGTGGTATCAACGCAGAGTACATG139141.0289137025807882No Hit
GGTATCAACGCAGAGTACTTTTTTT138801.0263994675737633No Hit
GTATCAACGCAGAGTACTTTTTTTT137401.0160467351918954No Hit
ATACCACTGCTTCCCATGTACTCTG134900.9975597130814168No Hit
GATACCACTGCTTCCCATGTACTCT132740.9815869259779635No Hit
GGGAAGCAGTGGTATCAACGCAGAG130140.9623604229830659No Hit
TATCAACGCAGAGTACTTTTTTTTT127480.9426902314575168No Hit
GTACTTTTTTTTTTTTTTTTTTTTT122790.9080085779782593No Hit
GCAGTGGTATCAACGCAGAGTACAT120560.8915181542557126No Hit
GCAGAGTACATGGGAAGCAGTGGTA117850.8714782222879538No Hit
GTTGATACCACTGCTTCCCATGTAC117280.8672631812467647No Hit
CTGCTTCCCATGTACTCTGCGTTGA115880.8569104488648968No Hit
TACCACTGCTTCCCATGTACTCTGC111210.822376691562523No Hit
CTTCCCATGTACTCTGCGTTGATAC110190.8148339865414479No Hit
CCACTGCTTCCCATGTACTCTGCGT103550.7657324558160172No Hit
AAGCAGTGGTATCAACGCAGAGTAC98450.728018930710641No Hit
CCATGTACTCTGCGTTGATACCACT97380.7201064852473563No Hit
ATGGGAAGCAGTGGTATCAACGCAG90710.6707831102565999No Hit
ACGCAGAGTACTTTTTTTTTTTTTT86280.6380241070768321No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA78470.5802706500036974No Hit
TTCCCATGTACTCTGCGTTGATACC77740.5748724395474377No Hit
GTACATGGTAAGCAGTGGTATCAAC70680.5226650891074466No Hit
TGATACCACTGCTTCCCATGTACTC69950.5172668786511869No Hit
GAGTACTTTTTTTTTTTTTTTTTTT68730.5082452118612734No Hit
ACCATGTACTCTGCGTTGATACCAC60390.44657250610071725No Hit
ATCAACGCAGAGTACATGGGAAGCA57300.4237225467721659No Hit
GGAAGCAGTGGTATCAACGCAGAGT43440.3212304961916735No Hit
CCCCATGTACTCTGCGTTGATACCA42270.31257856984396953No Hit
GGTAAGCAGTGGTATCAACGCAGAG38460.28440434814760035No Hit
GTACATGGGTAAGCAGTGGTATCAA35700.2639946757376322No Hit
AAAAAGTACTCTGCGTTGATACCAC35150.25992753087332693No Hit
TCCATGTACTCTGCGTTGATACCAC33000.24402869185831547No Hit
TCAACGCAGAGTACATGGGAAGCAG31860.2355986097759373No Hit
CTGCGTTGATACCACTGCTTCCCAT31850.2355246616874954No Hit
CTCTGCGTTGATACCACTGCTTCCC31040.22953486652370036No Hit
TGGGAAGCAGTGGTATCAACGCAGA30160.22302743474081196No Hit
GAGTACATGGTAAGCAGTGGTATCA29570.21866449752273903No Hit
AGTGGTATCAACGCAGAGTACATGG28250.20890334984840642No Hit
GCAGAGTACTTTTTTTTTTTTTTTT28240.2088294017599645No Hit
AAAGTACTCTGCGTTGATACCACTG27420.20276565850772757No Hit
GTGGTATCAACGCAGAGTACTTTTT27020.19980773497005103No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA26060.19270871847962728No Hit
AACGCAGAGTACATGGGAAGCAGTG25890.19145160097611477No Hit
GCTTACCATGTACTCTGCGTTGATA25380.18768024846557715No Hit
GTACATGGAAAGCAGTGGTATCAAC23970.1772535679952673No Hit
TCCCATGTACTCTGCGTTGATACCA23860.17644013902240627No Hit
ACTCTGCGTTGATACCACTGCTTAC22880.16919322635509873No Hit
ACGCAGAGTACATGGTAAGCAGTGG21320.15765732455816017No Hit
ACTGCTTCCCATGTACTCTGCGTTG20730.15329438734008727No Hit
GAGTACATGGAAAGCAGTGGTATCA19250.142350070250684No Hit
CAACGCAGAGTACATGGGAAGCAGT18300.13532500184870222No Hit
ATGTACTCTGCGTTGATACCACTGC18100.13384604007986395No Hit
GCGTTGATACCACTGCTTACCATGT17760.13133180507283887No Hit
TTGATACCACTGCTTCCCATGTACT17590.13007468756932633No Hit
ATGGTAAGCAGTGGTATCAACGCAG17300.12793019300451083No Hit
TATCAACGCAGAGTACATGGTAAGC16700.123493307697996No Hit
CGCAGAGTACATGGGAAGCAGTGGT16330.1207572284256452No Hit
CGTTGATACCACTGCTTCCCATGTA16270.12031353989499373No Hit
ATCAACGCAGAGTACTTTTTTTTTT16260.12023959180655179No Hit
GGTATCAACGCAGAGTACATGGTAA15960.1180211491532944No Hit
CTGCTTACCATGTACTCTGCGTTGA15820.11698587591510759No Hit
GCTTTCCATGTACTCTGCGTTGATA15540.114915329438734No Hit
ACGCAGAGTACATGGAAAGCAGTGG14270.10552392220661096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA2800.017.3318371
CGCAAAA1800.016.8877072
ATACGAC453.541587E-516.88708319
GGGACGC804.311005E-1016.6238357
GGACGCA804.311005E-1016.6238358
GAACTGT350.002171556216.283976
TGATACG456.7670125E-414.77619717
GATACGA456.7670125E-414.77619718
CGTACTC456.7670125E-414.7761975
TGGGACG902.2901077E-914.7761976
TACAAGA801.2890814E-714.24900152
TGAACTG400.005282384314.2484755
GAACAAA5600.014.1033581
CTGAGTA500.001500581613.2985764
GTACAAG1301.4551915E-1113.1753341
ACGCAAA3400.013.1538061
GACGCAG1051.9945219E-812.6657799
AACGCCG604.0951103E-412.6653126
CGCAGTG1155.326001E-912.39043611
TACAAAA3850.012.3367982
Produced by FastQC (version 0.11.5)