FastQCFastQC Report
Wed 25 May 2016
SRR1295014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4086289
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC346780.8486428639775602No Hit
CCCATGTACTCTGCGTTGATACCAC323630.7919899938550601No Hit
GAGTACATGGGAAGCAGTGGTATCA168150.4114980609545727No Hit
CATGTACTCTGCGTTGATACCACTG127310.3115540775505599No Hit
GCGTTGATACCACTGCTTCCCATGT100770.24660517158722742No Hit
GCTTCCCATGTACTCTGCGTTGATA99590.24371746589631815No Hit
ACGCAGAGTACATGGGAAGCAGTGG88770.21723867303560762No Hit
TATCAACGCAGAGTACATGGGAAGC84410.20656884522851907No Hit
GTATCAACGCAGAGTACATGGGAAG81400.19920274850848776No Hit
GGTATCAACGCAGAGTACATGGGAA74500.18231701184130636No Hit
ACTCTGCGTTGATACCACTGCTTCC74480.1822680676770537No Hit
GTATCAACGCAGAGTACTTTTTTTT51590.12625147168983886No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN47120.11531245097936048No Hit
GGTATCAACGCAGAGTACTTTTTTT44630.10921890252989937No Hit
GTGGTATCAACGCAGAGTACATGGG42350.10363926780509161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCG701.0927324E-412.2130968
TCGCGTT658.034892E-411.69116810
TACACCG803.7530006E-410.6938045
TACGACA856.5584615E-410.0653744
CTCGCGT856.6037494E-410.0578439
AGCACCG1356.802906E-69.1535445
ACACCGT1252.7620037E-59.1184416
GTCTTAG4400.09.0787691
GTCCTAA3900.09.0233421
CTTAGAC2850.09.0046453
GGTGCGT950.00182639968.9991238
CTACGCT850.0073994558.9474
GTCTAGG4700.08.9041
CTAGGAC3950.08.9033273
ACTGTTC4400.08.851418
CGTTCCG1856.5938366E-88.72887910
GTACTAG2401.0004442E-108.71851
GTGTAGA5350.08.7110931
GTATTAA7150.08.5134241
CTAGTAC2356.1118044E-108.4937443
Produced by FastQC (version 0.11.5)