FastQCFastQC Report
Wed 25 May 2016
SRR1295014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4086289
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC355200.8692483571279467No Hit
CCCATGTACTCTGCGTTGATACCAC353850.8659446260408894No Hit
GAGTACATGGGAAGCAGTGGTATCA187070.4577992403376267No Hit
CATGTACTCTGCGTTGATACCACTG143990.3523735105373115No Hit
GTATCAACGCAGAGTACTTTTTTTT136190.3332852864787586No Hit
GGTATCAACGCAGAGTACTTTTTTT130870.3202661387875405No Hit
ACGCAGAGTACATGGGAAGCAGTGG111410.2726434669696637No Hit
GCGTTGATACCACTGCTTCCCATGT108140.2646410961143473No Hit
GCTTCCCATGTACTCTGCGTTGATA106260.2600403446745935No Hit
TATCAACGCAGAGTACTTTTTTTTT105990.25937959845718206No Hit
TATCAACGCAGAGTACATGGGAAGC97280.23806441492513133No Hit
GTATCAACGCAGAGTACATGGGAAG91090.2229161960889208No Hit
GGTATCAACGCAGAGTACATGGGAA87910.21513407397274154No Hit
ACTCTGCGTTGATACCACTGCTTCC82990.20309380956657738No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59740.14619621862281398No Hit
GTGGTATCAACGCAGAGTACATGGG48360.11834698916302787No Hit
CAGTGGTATCAACGCAGAGTACATG46850.11465170476194905No Hit
ACATGGGAAGCAGTGGTATCAACGC45640.11169058282466073No Hit
CATGGGAAGCAGTGGTATCAACGCA41420.10136336416734107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT951.36270555E-510.9991866
CTAGACT4700.010.1056354
GTCCTAC1901.027729E-99.5130321
TACACCG800.004520879.4992965
TAGACTC3550.09.3655045
TCTAGAC4200.09.2731233
CTGTCGC3300.09.2114399
AATCCGA1054.4987627E-49.04694911
CTTACAC6200.09.0396533
GTCTAAG2950.09.0293191
GTGTAGG4250.08.9534431
CGTATAC850.0074436028.9405153
CTAAGAC4600.08.8797773
GTCTAAC2252.5465852E-108.878831
GTCTCGC2800.08.8335291
CTTAGAC3250.08.7685813
ACGCAAA2303.947207E-108.6858121
TAGACAG6650.08.5707945
TTACACC4000.08.5493674
GTATTAG5800.08.5289251
Produced by FastQC (version 0.11.5)