FastQCFastQC Report
Wed 25 May 2016
SRR1295013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965551
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1785969.08630709658513No Hit
CCCATGTACTCTGCGTTGATACCAC1748188.894096362801067No Hit
GAGTACATGGGAAGCAGTGGTATCA849904.323978365354041No Hit
CATGTACTCTGCGTTGATACCACTG691853.5198781410403495No Hit
GCGTTGATACCACTGCTTCCCATGT539662.7455914397540435No Hit
ACGCAGAGTACATGGGAAGCAGTGG475272.417998820686922No Hit
TATCAACGCAGAGTACATGGGAAGC475102.4171339232612126No Hit
ACTCTGCGTTGATACCACTGCTTCC444012.258959446994761No Hit
GGTATCAACGCAGAGTACATGGGAA430782.1916500767469276No Hit
GTATCAACGCAGAGTACATGGGAAG391951.9940973294511308No Hit
GCTTCCCATGTACTCTGCGTTGATA299761.5250685431209876No Hit
GTACTCTGCGTTGATACCACTGCTT219631.1173965976970326No Hit
GTGGTATCAACGCAGAGTACATGGG208611.0613308939834174No Hit
ATACCACTGCTTCCCATGTACTCTG201011.0226648914223035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA190720.9703131590073216No Hit
ACATGGGAAGCAGTGGTATCAACGC187320.953015210493139No Hit
GTTGATACCACTGCTTCCCATGTAC182450.9282384430625306No Hit
CAGTGGTATCAACGCAGAGTACATG179650.9139930736979097No Hit
GATACCACTGCTTCCCATGTACTCT177140.9012231175889103No Hit
GGGAAGCAGTGGTATCAACGCAGAG175560.8931846591617313No Hit
TACCACTGCTTCCCATGTACTCTGC170450.8671868600712981No Hit
CATGGGAAGCAGTGGTATCAACGCA167790.8536537591749083No Hit
CTGCTTCCCATGTACTCTGCGTTGA166660.8479047351099004No Hit
GCAGTGGTATCAACGCAGAGTACAT164010.8344225105326699No Hit
GCAGAGTACATGGGAAGCAGTGGTA151070.7685885535404576No Hit
CTTCCCATGTACTCTGCGTTGATAC148980.7579554028361514No Hit
CCACTGCTTCCCATGTACTCTGCGT147950.7527151419627373No Hit
CCATGTACTCTGCGTTGATACCACT141000.7173561001469817No Hit
AAGCAGTGGTATCAACGCAGAGTAC133530.6793514897349395No Hit
TTCCCATGTACTCTGCGTTGATACC126130.6417030135570128No Hit
GTACATGGTAAGCAGTGGTATCAAC120430.6127035116361773No Hit
ATGGGAAGCAGTGGTATCAACGCAG119190.6063948480604167No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA119010.6054790743155481No Hit
TGATACCACTGCTTCCCATGTACTC108350.55124491809167No Hit
ACCATGTACTCTGCGTTGATACCAC98640.5018440121879311No Hit
ATCAACGCAGAGTACATGGGAAGCA72940.3710918719483748No Hit
GAACAAAAAAAAAAAAAAAAAAAAA65040.33089957981248No Hit
CCCCATGTACTCTGCGTTGATACCA58780.2990510040187204No Hit
TCCATGTACTCTGCGTTGATACCAC54310.27630928935448634No Hit
GGTAAGCAGTGGTATCAACGCAGAG53980.27463037082222747No Hit
GGAAGCAGTGGTATCAACGCAGAGT53910.27427423658811195No Hit
GTACATGGGTAAGCAGTGGTATCAA53000.2696444915446101No Hit
AAAAAGTACTCTGCGTTGATACCAC48340.24593612681634822No Hit
GAGTACATGGTAAGCAGTGGTATCA43670.22217688576892689No Hit
CTCTGCGTTGATACCACTGCTTCCC43020.21886992502356847No Hit
CTGCGTTGATACCACTGCTTCCCAT42620.21683487225719403No Hit
GCTTACCATGTACTCTGCGTTGATA38980.19831589208318687No Hit
TCAACGCAGAGTACATGGGAAGCAG38500.19587382876353754No Hit
GTACATGGAAAGCAGTGGTATCAAC38420.19546681821026266No Hit
TGGGAAGCAGTGGTATCAACGCAGA37840.19251599169901976No Hit
TCCCATGTACTCTGCGTTGATACCA37090.18870026776206772No Hit
AAAGTACTCTGCGTTGATACCACTG35390.18005129350497645No Hit
AGTGGTATCAACGCAGAGTACATGG34580.17593031165306827No Hit
ACTCTGCGTTGATACCACTGCTTAC34030.17313211409930346No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31570.16061653958610078No Hit
GCGTTGATACCACTGCTTACCATGT30900.15720782620242366No Hit
ACTGCTTCCCATGTACTCTGCGTTG28280.1438782305826712No Hit
AACGCAGAGTACATGGGAAGCAGTG28200.14347122002939633No Hit
TTGATACCACTGCTTCCCATGTACT28110.1430133331569621No Hit
GAGTACATGGAAAGCAGTGGTATCA27720.14102915670974706No Hit
ACGCAGAGTACATGGTAAGCAGTGG26990.13731518541111373No Hit
GTATCAACGCAGAGTACTTTTTTTT26580.13522925632557997No Hit
ATGTACTCTGCGTTGATACCACTGC25910.13182054294190282No Hit
TATCAACGCAGAGTACATGGTAAGC25660.1305486349629188No Hit
CTGCTTACCATGTACTCTGCGTTGA25330.1288697164306599No Hit
TATCAACGCAGAGTACTTTTTTTTT25310.12876796379234118No Hit
GGTATCAACGCAGAGTACATGGTAA25150.1279539426857914No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA24770.12602064255773573No Hit
GGTATCAACGCAGAGTACTTTTTTT24770.12602064255773573No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN23760.12088213432264032No Hit
ATGGTAAGCAGTGGTATCAACGCAG23620.12016986585440927No Hit
GTACATGGAAGCAGTGGTATCAACG22630.11513311025763259No Hit
CGTTGATACCACTGCTTCCCATGTA22350.1137085733211705No Hit
GCTTTCCATGTACTCTGCGTTGATA21520.10948583883094358No Hit
GTATCAACGCAGAGTACATGGTAAG21130.10750166238372852No Hit
AAACAAAAAAAAAAAAAAAAAAAAA21110.10739990974540982No Hit
CAACGCAGAGTACATGGGAAGCAGT19840.10093861721217105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAG250.00602061519.0065573
GTACAAA5950.016.9431841
GTCTTGC402.738272E-416.643481
ATAGCGT402.7645208E-416.6226796
GTGACAA402.7677498E-416.62013613
TATAGCG350.00216362916.2934155
TAGCGTG350.002170647316.2855177
TACATAA4200.015.6125312
AGAACAA11600.015.4018662
TACATGA4750.014.805112
GTACCTG653.3345423E-614.6316321
CGTGTGA400.00528017614.24982810
TGACAAA400.00528822414.24655714
TGTGACA400.005289118714.24619512
TCATTCT400.005290908714.24546715
TACAAAA7550.013.8458372
GTACATA5500.013.8335421
TACAAGA1351.8189894E-1213.3749852
TTGCTCT500.001493883213.3069684
CGCAGAA3500.013.3045912
Produced by FastQC (version 0.11.5)