##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295013_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1965551 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.16157759325502 33.0 33.0 33.0 22.0 33.0 2 29.96427871879183 33.0 33.0 33.0 15.0 33.0 3 30.376196293049635 33.0 33.0 33.0 22.0 33.0 4 33.23006882039693 37.0 33.0 37.0 15.0 37.0 5 34.37411901293836 37.0 33.0 37.0 27.0 37.0 6 33.8399400473455 37.0 37.0 37.0 27.0 37.0 7 34.12226597020378 37.0 37.0 37.0 27.0 37.0 8 33.96837019237863 37.0 37.0 37.0 27.0 37.0 9 33.758604584668625 37.0 37.0 37.0 22.0 37.0 10 33.96975402825976 37.0 33.0 37.0 27.0 37.0 11 34.1232087083978 37.0 37.0 37.0 27.0 37.0 12 34.20060125633982 37.0 37.0 37.0 27.0 37.0 13 33.74344496784871 37.0 33.0 37.0 27.0 37.0 14 35.24144120401862 37.0 33.0 40.0 27.0 40.0 15 35.115813835407984 37.0 33.0 40.0 27.0 40.0 16 35.06796567476499 37.0 33.0 40.0 27.0 40.0 17 35.210609645844855 37.0 33.0 40.0 27.0 40.0 18 35.01891225412111 37.0 33.0 40.0 27.0 40.0 19 34.78439582590327 37.0 33.0 40.0 22.0 40.0 20 34.971447192161385 37.0 33.0 40.0 27.0 40.0 21 34.855852633688976 37.0 33.0 40.0 22.0 40.0 22 34.957535062687256 37.0 33.0 40.0 22.0 40.0 23 34.95633234650233 37.0 33.0 40.0 22.0 40.0 24 35.1098455344074 37.0 33.0 40.0 27.0 40.0 25 33.89401852203275 37.0 33.0 40.0 15.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7423451714597995 1101 2 0.6729124612750752 1101 3 0.50557295634394 1101 4 0.6109631478290822 1101 5 0.3380172299633628 1101 6 0.21261885034564187 1101 7 0.3274812284405684 1101 8 0.3361483059707169 1101 9 0.5164773110051755 1101 10 0.4966656435768684 1101 11 0.4009809860547122 1101 12 0.3738825209293708 1101 13 0.46527057927554694 1101 14 1.025380465593365 1101 15 0.8196367140394187 1101 16 1.0468326738886162 1101 17 0.7755238632620731 1101 18 1.0673065774344863 1101 19 1.0532371579964064 1101 20 0.9438947142288825 1101 21 0.8933415218507932 1101 22 1.0465285523429912 1101 23 0.9813027895008304 1101 24 1.0678496455709094 1101 25 1.1300306258030233 1102 1 0.6851234467779683 1102 2 0.6447492726803894 1102 3 0.6295281918971298 1102 4 0.8875188830697311 1102 5 0.4992449787313973 1102 6 0.5315590589027153 1102 7 0.5146913961467803 1102 8 0.5501801612431052 1102 9 0.5465010116449349 1102 10 0.8578262553589937 1102 11 0.6224473447605732 1102 12 0.6272568526421622 1102 13 0.6709192073534211 1102 14 1.1665818663037655 1102 15 1.0603006247339408 1102 16 0.951573713966873 1102 17 0.9137349542425994 1102 18 0.9716103600214581 1102 19 0.9698224371143169 1102 20 1.0267739833028244 1102 21 0.9709431842175178 1102 22 0.9882382888162411 1102 23 1.205218037895854 1102 24 1.0896972054252458 1102 25 1.3082254967039617 1103 1 0.6697826050944968 1103 2 0.6170504011545788 1103 3 0.5895681552662637 1103 4 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-0.32910548772505877 2214 21 -0.2153619709291803 2214 22 -0.5691670315271438 2214 23 -1.8965817885513587 2214 24 -1.4268521483078516 2214 25 -1.8089366441136931 2215 1 -0.5022773595077261 2215 2 -0.15464235961545114 2215 3 -1.2026522696353013 2215 4 -0.9846909365365022 2215 5 -0.32672791566770343 2215 6 -0.33188650619938187 2215 7 -0.29331304675192627 2215 8 -0.27406888519097805 2215 9 -0.4073802383754739 2215 10 -1.5860260202999328 2215 11 -0.6632661682807424 2215 12 -0.6172561633692553 2215 13 -0.45945531627182135 2215 14 -0.8173651822137984 2215 15 -1.3025803934262612 2215 16 -0.8762925353514248 2215 17 -0.646450525757011 2215 18 -0.5862128400412416 2215 19 -1.1943461195395813 2215 20 -1.3821849643784105 2215 21 -0.6908148223072246 2215 22 -0.35927158157063843 2215 23 -0.7199652051426213 2215 24 -1.8593871304585505 2215 25 -0.745293696259246 2216 1 -0.8109163933947947 2216 2 -0.809605654295197 2216 3 -1.7262885192739894 2216 4 -1.1942801217506585 2216 5 -1.02611654998325 2216 6 -0.9861897639825301 2216 7 -0.8010649468420468 2216 8 -0.8986326508550349 2216 9 -1.0207660969441577 2216 10 -0.8555378629197605 2216 11 -0.9878263207980424 2216 12 -0.9507807455194595 2216 13 -1.6489169361517853 2216 14 -1.3365281436338918 2216 15 -1.6226654511515974 2216 16 -1.5700060850768267 2216 17 -2.418496459479144 2216 18 -1.4174229891579344 2216 19 -1.2871416111825837 2216 20 -1.2503807714278778 2216 21 -0.9143721140624521 2216 22 -0.944144398804923 2216 23 -0.7583918710158173 2216 24 -0.9714355933834184 2216 25 -1.012073893664585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2645.0 3 300.0 4 316.0 5 307.0 6 185.0 7 141.0 8 192.0 9 309.0 10 499.0 11 1088.0 12 2123.0 13 4075.0 14 7797.0 15 14404.0 16 21613.0 17 23939.0 18 22457.0 19 21477.0 20 21092.0 21 21308.0 22 21241.0 23 20457.0 24 20702.0 25 20266.0 26 19999.0 27 20658.0 28 21606.0 29 24623.0 30 28933.0 31 37098.0 32 49138.0 33 69758.0 34 101726.0 35 161644.0 36 298771.0 37 882664.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.255748377419586 18.101959855648143 9.87103566997503 23.77125609695725 2 9.236693553525237 28.745594250544748 30.303655848210305 31.714056347719715 3 23.736334323184177 35.096950154850056 24.040171800721357 17.12654372124441 4 13.820774632805122 26.327684076765706 22.960719683603557 36.89082160682561 5 8.543811034963245 46.39348300749901 33.595592094290744 11.467113863247 6 32.118560992059265 27.70866670371018 19.53333329934846 20.639439004882096 7 19.491159521133294 38.547597382902204 21.64794580252483 20.313297293439668 8 29.64539433799584 36.46028627675527 21.260788405755722 12.633530979493171 9 27.98796269291406 21.905726796775095 16.552520961271362 33.55378954903948 10 18.216581881376744 38.145527456971635 27.91530173908432 15.7225889225673 11 22.928462113356602 33.40230897621385 12.792356295646021 30.876872614783522 12 24.426719281340656 40.408120605884484 23.284338467342707 11.880821645432158 13 31.30948010361215 24.119092781823003 17.050470129923106 27.520956984641742 14 20.10971009268611 36.79688133332586 21.182134151408903 21.91127442257913 15 41.21675632246724 21.219774303927046 17.129083961059884 20.43438541254583 16 13.872569212196357 29.878297469696726 37.089651614271496 19.159481703835425 17 29.306014951731115 35.085841942338355 23.76095583407086 11.847187271859667 18 29.856866458827756 24.153294365662227 27.72130892628177 18.268530249228252 19 22.668767831178126 38.61511778009352 28.145119024069924 10.570995364658426 20 24.314337844513403 29.830171836757373 24.25504863193838 21.60044168679084 21 15.30796653536182 34.688847278716835 31.16502760766377 18.83815857825757 22 20.64372890687929 33.792836240912756 13.70616964221989 31.85726520998807 23 12.512133744453147 34.689865570347166 25.9816607119551 26.816339973244585 24 19.871992624686598 46.073629736219345 16.98183247779222 17.072545161301836 25 20.30315900679199 26.507828302012815 14.296421573451228 38.89259111774396 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 26190.0 1 26190.0 2 27755.5 3 29321.0 4 29321.0 5 29321.0 6 32858.5 7 36396.0 8 36396.0 9 36396.0 10 33564.5 11 30733.0 12 30733.0 13 30733.0 14 28607.0 15 26481.0 16 26481.0 17 26481.0 18 25706.5 19 24932.0 20 24932.0 21 24932.0 22 25111.0 23 25290.0 24 25290.0 25 25290.0 26 26923.5 27 28557.0 28 28557.0 29 28557.0 30 28760.0 31 28963.0 32 28963.0 33 28963.0 34 29821.5 35 30680.0 36 30680.0 37 30680.0 38 34564.5 39 38449.0 40 38449.0 41 38449.0 42 79510.0 43 120571.0 44 120571.0 45 120571.0 46 471799.5 47 823028.0 48 823028.0 49 823028.0 50 672010.5 51 520993.0 52 520993.0 53 520993.0 54 325890.0 55 130787.0 56 130787.0 57 130787.0 58 75611.0 59 20435.0 60 20435.0 61 20435.0 62 15929.5 63 11424.0 64 11424.0 65 11424.0 66 8903.5 67 6383.0 68 6383.0 69 6383.0 70 4945.5 71 3508.0 72 3508.0 73 3508.0 74 2554.5 75 1601.0 76 1601.0 77 1601.0 78 1069.5 79 538.0 80 538.0 81 538.0 82 371.0 83 204.0 84 204.0 85 204.0 86 130.0 87 56.0 88 56.0 89 56.0 90 37.5 91 19.0 92 19.0 93 19.0 94 12.5 95 6.0 96 6.0 97 6.0 98 6.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3140086418515724 2 0.18447753327184083 3 0.202284244977617 4 0.22726451768486292 5 0.2488869533275911 6 0.1981123868065494 7 0.1951615602953065 8 0.21886992502356847 9 0.21953131717264013 10 0.22991008628114967 11 0.20294563712668864 12 0.20966131125572424 13 0.22339791742875154 14 0.2136805404693137 15 0.2022333686584576 16 0.20589646363793154 17 0.20548945308465666 18 0.20838940327674021 19 0.2085420322342183 20 0.20248775025425442 21 0.20136847123274848 22 0.20406491614819458 23 0.2075245058510311 24 0.22456807276941682 25 0.2172418828104689 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1965551.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.520095502459425 #Duplication Level Percentage of deduplicated Percentage of total 1 76.86203673083584 8.85458003652773 2 10.841735686835264 2.4979566104952955 3 4.301573068977891 1.4866359769629838 4 2.209608553334554 1.0181960622986108 5 1.2988049578256409 0.7481178576609584 6 0.8367359317454005 0.5783566706427831 7 0.5704427776626697 0.46000886941535285 8 0.4093741804113679 0.377282372366401 9 0.31019386529951304 0.3216116657274682 >10 1.8628786306460614 4.150554292425726 >50 0.2294798298936836 1.8356383760485502 >100 0.19138086199317011 4.486770124230159 >500 0.023479596494532445 1.8606456826387212 >1k 0.0336688553506503 8.296760875003956 >5k 0.0035440900369105577 2.605863419703031 >10k+ 0.01506238265686987 60.42102110785227 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 178596 9.08630709658513 No Hit CCCATGTACTCTGCGTTGATACCAC 174818 8.894096362801067 No Hit GAGTACATGGGAAGCAGTGGTATCA 84990 4.323978365354041 No Hit CATGTACTCTGCGTTGATACCACTG 69185 3.5198781410403495 No Hit GCGTTGATACCACTGCTTCCCATGT 53966 2.7455914397540435 No Hit ACGCAGAGTACATGGGAAGCAGTGG 47527 2.417998820686922 No Hit TATCAACGCAGAGTACATGGGAAGC 47510 2.4171339232612126 No Hit ACTCTGCGTTGATACCACTGCTTCC 44401 2.258959446994761 No Hit GGTATCAACGCAGAGTACATGGGAA 43078 2.1916500767469276 No Hit GTATCAACGCAGAGTACATGGGAAG 39195 1.9940973294511308 No Hit GCTTCCCATGTACTCTGCGTTGATA 29976 1.5250685431209876 No Hit GTACTCTGCGTTGATACCACTGCTT 21963 1.1173965976970326 No Hit GTGGTATCAACGCAGAGTACATGGG 20861 1.0613308939834174 No Hit ATACCACTGCTTCCCATGTACTCTG 20101 1.0226648914223035 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 19072 0.9703131590073216 No Hit ACATGGGAAGCAGTGGTATCAACGC 18732 0.953015210493139 No Hit GTTGATACCACTGCTTCCCATGTAC 18245 0.9282384430625306 No Hit CAGTGGTATCAACGCAGAGTACATG 17965 0.9139930736979097 No Hit GATACCACTGCTTCCCATGTACTCT 17714 0.9012231175889103 No Hit GGGAAGCAGTGGTATCAACGCAGAG 17556 0.8931846591617313 No Hit TACCACTGCTTCCCATGTACTCTGC 17045 0.8671868600712981 No Hit CATGGGAAGCAGTGGTATCAACGCA 16779 0.8536537591749083 No Hit CTGCTTCCCATGTACTCTGCGTTGA 16666 0.8479047351099004 No Hit GCAGTGGTATCAACGCAGAGTACAT 16401 0.8344225105326699 No Hit GCAGAGTACATGGGAAGCAGTGGTA 15107 0.7685885535404576 No Hit CTTCCCATGTACTCTGCGTTGATAC 14898 0.7579554028361514 No Hit CCACTGCTTCCCATGTACTCTGCGT 14795 0.7527151419627373 No Hit CCATGTACTCTGCGTTGATACCACT 14100 0.7173561001469817 No Hit AAGCAGTGGTATCAACGCAGAGTAC 13353 0.6793514897349395 No Hit TTCCCATGTACTCTGCGTTGATACC 12613 0.6417030135570128 No Hit GTACATGGTAAGCAGTGGTATCAAC 12043 0.6127035116361773 No Hit ATGGGAAGCAGTGGTATCAACGCAG 11919 0.6063948480604167 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 11901 0.6054790743155481 No Hit TGATACCACTGCTTCCCATGTACTC 10835 0.55124491809167 No Hit ACCATGTACTCTGCGTTGATACCAC 9864 0.5018440121879311 No Hit ATCAACGCAGAGTACATGGGAAGCA 7294 0.3710918719483748 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 6504 0.33089957981248 No Hit CCCCATGTACTCTGCGTTGATACCA 5878 0.2990510040187204 No Hit TCCATGTACTCTGCGTTGATACCAC 5431 0.27630928935448634 No Hit GGTAAGCAGTGGTATCAACGCAGAG 5398 0.27463037082222747 No Hit GGAAGCAGTGGTATCAACGCAGAGT 5391 0.27427423658811195 No Hit GTACATGGGTAAGCAGTGGTATCAA 5300 0.2696444915446101 No Hit AAAAAGTACTCTGCGTTGATACCAC 4834 0.24593612681634822 No Hit GAGTACATGGTAAGCAGTGGTATCA 4367 0.22217688576892689 No Hit CTCTGCGTTGATACCACTGCTTCCC 4302 0.21886992502356847 No Hit CTGCGTTGATACCACTGCTTCCCAT 4262 0.21683487225719403 No Hit GCTTACCATGTACTCTGCGTTGATA 3898 0.19831589208318687 No Hit TCAACGCAGAGTACATGGGAAGCAG 3850 0.19587382876353754 No Hit GTACATGGAAAGCAGTGGTATCAAC 3842 0.19546681821026266 No Hit TGGGAAGCAGTGGTATCAACGCAGA 3784 0.19251599169901976 No Hit TCCCATGTACTCTGCGTTGATACCA 3709 0.18870026776206772 No Hit AAAGTACTCTGCGTTGATACCACTG 3539 0.18005129350497645 No Hit AGTGGTATCAACGCAGAGTACATGG 3458 0.17593031165306827 No Hit ACTCTGCGTTGATACCACTGCTTAC 3403 0.17313211409930346 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3157 0.16061653958610078 No Hit GCGTTGATACCACTGCTTACCATGT 3090 0.15720782620242366 No Hit ACTGCTTCCCATGTACTCTGCGTTG 2828 0.1438782305826712 No Hit AACGCAGAGTACATGGGAAGCAGTG 2820 0.14347122002939633 No Hit TTGATACCACTGCTTCCCATGTACT 2811 0.1430133331569621 No Hit GAGTACATGGAAAGCAGTGGTATCA 2772 0.14102915670974706 No Hit ACGCAGAGTACATGGTAAGCAGTGG 2699 0.13731518541111373 No Hit GTATCAACGCAGAGTACTTTTTTTT 2658 0.13522925632557997 No Hit ATGTACTCTGCGTTGATACCACTGC 2591 0.13182054294190282 No Hit TATCAACGCAGAGTACATGGTAAGC 2566 0.1305486349629188 No Hit CTGCTTACCATGTACTCTGCGTTGA 2533 0.1288697164306599 No Hit TATCAACGCAGAGTACTTTTTTTTT 2531 0.12876796379234118 No Hit GGTATCAACGCAGAGTACATGGTAA 2515 0.1279539426857914 No Hit TAAAAAAAAAAAAAAAAAAAAAAAA 2477 0.12602064255773573 No Hit GGTATCAACGCAGAGTACTTTTTTT 2477 0.12602064255773573 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 2376 0.12088213432264032 No Hit ATGGTAAGCAGTGGTATCAACGCAG 2362 0.12016986585440927 No Hit GTACATGGAAGCAGTGGTATCAACG 2263 0.11513311025763259 No Hit CGTTGATACCACTGCTTCCCATGTA 2235 0.1137085733211705 No Hit GCTTTCCATGTACTCTGCGTTGATA 2152 0.10948583883094358 No Hit GTATCAACGCAGAGTACATGGTAAG 2113 0.10750166238372852 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 2111 0.10739990974540982 No Hit CAACGCAGAGTACATGGGAAGCAGT 1984 0.10093861721217105 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.0876319159360404E-5 0.0 2 0.0 0.0 0.0 5.0876319159360404E-5 0.0 3 0.0 0.0 0.0 5.0876319159360404E-5 0.0 4 0.0 0.0 0.0 5.0876319159360404E-5 0.0 5 0.0 0.0 0.0 5.0876319159360404E-5 0.0 6 0.0 0.0 0.0 5.0876319159360404E-5 0.0 7 0.0 0.0 0.0 5.0876319159360404E-5 0.0 8 0.0 0.0 0.0 5.0876319159360404E-5 0.0 9 0.0 0.0 0.0 5.0876319159360404E-5 0.0 10 0.0 0.0 0.0 6.105158299123248E-4 0.0 11 0.0 0.0 0.0 6.613921490716852E-4 0.0 12 0.0 0.0 0.0 9.157737448684872E-4 0.0 13 0.0 0.0 0.0 9.666500640278477E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTATAG 25 0.006020615 19.006557 3 GTACAAA 595 0.0 16.943184 1 GTCTTGC 40 2.738272E-4 16.64348 1 ATAGCGT 40 2.7645208E-4 16.622679 6 GTGACAA 40 2.7677498E-4 16.620136 13 TATAGCG 35 0.002163629 16.293415 5 TAGCGTG 35 0.0021706473 16.285517 7 TACATAA 420 0.0 15.612531 2 AGAACAA 1160 0.0 15.401866 2 TACATGA 475 0.0 14.80511 2 GTACCTG 65 3.3345423E-6 14.631632 1 CGTGTGA 40 0.005280176 14.249828 10 TGACAAA 40 0.005288224 14.246557 14 TGTGACA 40 0.0052891187 14.246195 12 TCATTCT 40 0.0052909087 14.245467 15 TACAAAA 755 0.0 13.845837 2 GTACATA 550 0.0 13.833542 1 TACAAGA 135 1.8189894E-12 13.374985 2 TTGCTCT 50 0.0014938832 13.306968 4 CGCAGAA 350 0.0 13.304591 2 >>END_MODULE