FastQCFastQC Report
Wed 25 May 2016
SRR1295013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965551
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1922459.78071797679124No Hit
GTACATGGGAAGCAGTGGTATCAAC1785009.081422969945832No Hit
GAGTACATGGGAAGCAGTGGTATCA942654.795856225557108No Hit
CATGTACTCTGCGTTGATACCACTG773083.933146481571834No Hit
ACGCAGAGTACATGGGAAGCAGTGG597473.039707440814306No Hit
GCGTTGATACCACTGCTTCCCATGT581352.957694814329417No Hit
TATCAACGCAGAGTACATGGGAAGC536422.729107512346411No Hit
ACTCTGCGTTGATACCACTGCTTCC489642.4911080913189227No Hit
GGTATCAACGCAGAGTACATGGGAA482072.4525947177152867No Hit
GTATCAACGCAGAGTACATGGGAAG424092.157613819229315No Hit
GCTTCCCATGTACTCTGCGTTGATA329011.6738817766621166No Hit
ACATGGGAAGCAGTGGTATCAACGC245891.2509978118095129No Hit
GTGGTATCAACGCAGAGTACATGGG235201.1966110266281567No Hit
CAGTGGTATCAACGCAGAGTACATG226431.1519924947253977No Hit
CATGGGAAGCAGTGGTATCAACGCA213851.0879900852229223No Hit
ATACCACTGCTTCCCATGTACTCTG211431.075678015986357No Hit
GTACTCTGCGTTGATACCACTGCTT208621.0613817703025767No Hit
GGGAAGCAGTGGTATCAACGCAGAG206181.0489679484276928No Hit
GCAGTGGTATCAACGCAGAGTACAT199851.0167632383998177No Hit
GATACCACTGCTTCCCATGTACTCT191400.9737727487101581No Hit
GTTGATACCACTGCTTCCCATGTAC183260.9323594249144387No Hit
GCAGAGTACATGGGAAGCAGTGGTA182180.9268647824452279No Hit
CTGCTTCCCATGTACTCTGCGTTGA179430.9128737946764036No Hit
TACCACTGCTTCCCATGTACTCTGC177410.902596778206213No Hit
CTTCCCATGTACTCTGCGTTGATAC171520.8726306262213497No Hit
CCATGTACTCTGCGTTGATACCACT164850.8386961213420563No Hit
AAGCAGTGGTATCAACGCAGAGTAC161070.819464872699818No Hit
CCACTGCTTCCCATGTACTCTGCGT159620.8120878064217107No Hit
GTACTTTTTTTTTTTTTTTTTTTTT156340.7954003737374405No Hit
ATGGGAAGCAGTGGTATCAACGCAG143620.730685695766734No Hit
GTACATGGTAAGCAGTGGTATCAAC128580.654167711751056No Hit
TTCCCATGTACTCTGCGTTGATACC126990.6460783770047177No Hit
TATCAACGCAGAGTACTTTTTTTTT125590.6389556923224073No Hit
GTATCAACGCAGAGTACTTTTTTTT119970.6103632009548468No Hit
GGTATCAACGCAGAGTACTTTTTTT119710.6090404166567034No Hit
TGATACCACTGCTTCCCATGTACTC109860.5589272422847333No Hit
ACCATGTACTCTGCGTTGATACCAC105940.5389837251742641No Hit
ATCAACGCAGAGTACATGGGAAGCA90060.4581921303491998No Hit
ACGCAGAGTACTTTTTTTTTTTTTT83830.42649618351291824No Hit
GAGTACTTTTTTTTTTTTTTTTTTT83290.4237488622783128No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA74070.3768408960133825No Hit
CCCCATGTACTCTGCGTTGATACCA66690.3392941724737745No Hit
GGAAGCAGTGGTATCAACGCAGAGT65680.3341556642386791No Hit
GGTAAGCAGTGGTATCAACGCAGAG64030.32576107157738465No Hit
TCCATGTACTCTGCGTTGATACCAC57270.291368679825657No Hit
GTACATGGGTAAGCAGTGGTATCAA57140.2907072876765854No Hit
AAAAAGTACTCTGCGTTGATACCAC50850.25870608292534764No Hit
GAGTACATGGTAAGCAGTGGTATCA50390.2563657722440171No Hit
CTGCGTTGATACCACTGCTTCCCAT47450.24140813441116513No Hit
CTCTGCGTTGATACCACTGCTTCCC45720.23260653119659577No Hit
TCAACGCAGAGTACATGGGAAGCAG45690.23245390223911766No Hit
TGGGAAGCAGTGGTATCAACGCAGA45040.22914694149375925No Hit
AGTGGTATCAACGCAGAGTACATGG41520.2112384771496644No Hit
GCTTACCATGTACTCTGCGTTGATA41280.21001744548983975No Hit
TCCCATGTACTCTGCGTTGATACCA39050.19867202631730238No Hit
AAAGTACTCTGCGTTGATACCACTG38710.19694223146588413No Hit
GTACATGGAAAGCAGTGGTATCAAC38070.19368614703968506No Hit
ACTCTGCGTTGATACCACTGCTTAC37680.19170197059247002No Hit
AACGCAGAGTACATGGGAAGCAGTG35750.18188284099471344No Hit
GCGTTGATACCACTGCTTACCATGT32740.16656906892774595No Hit
ACGCAGAGTACATGGTAAGCAGTGG32680.1662638110127898No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA32420.16494102671464642No Hit
GAGTACATGGAAAGCAGTGGTATCA31590.1607182922244195No Hit
ACTGCTTCCCATGTACTCTGCGTTG29980.15252720483976248No Hit
ATGGTAAGCAGTGGTATCAACGCAG28100.14296245683780273No Hit
TATCAACGCAGAGTACATGGTAAGC28100.14296245683780273No Hit
GCAGAGTACTTTTTTTTTTTTTTTT27560.14021513560319726No Hit
CTGCTTACCATGTACTCTGCGTTGA27040.13756956700691053No Hit
GTGGTATCAACGCAGAGTACTTTTT26940.13706080381531693No Hit
TTGATACCACTGCTTCCCATGTACT26640.1355345142405361No Hit
CAACGCAGAGTACATGGGAAGCAGT26280.13370296675079912No Hit
CGTTGATACCACTGCTTCCCATGTA25920.13187141926106216No Hit
CGCAGAGTACATGGGAAGCAGTGGT25810.1313117797503092No Hit
ATGTACTCTGCGTTGATACCACTGC25710.1308030165587156No Hit
GGTATCAACGCAGAGTACATGGTAA25500.12973461385636903No Hit
GTACATGGAAGCAGTGGTATCAACG24670.12551187936614214No Hit
GCTTTCCATGTACTCTGCGTTGATA24660.12546100304698277No Hit
ACGCAGAGTACATGGAAAGCAGTGG22730.11564187344922619No Hit
GTATCAACGCAGAGTACATGGTAAG21970.11177527319311481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACGC1250.017.4780587
GTACAAA3550.017.4274561
GGACGCA1300.016.8058268
TACAAGA804.3291948E-1016.6235752
CGCAAAA2450.015.8965972
TACAAAA3650.015.8753522
TGGGACG1450.015.067296
ACCCACT704.3432192E-714.956971
GAACAAG1350.014.8058891
TGATACG456.769155E-414.77613517
GAACAAA5100.014.743681
GTACAAG1700.014.5570521
ACGCAAA3200.014.2771091
CCAGAGC400.005282762514.2487793
AGGTGTC400.005283653714.2484177
ACAAGAA1750.014.1130763
GACGCAG1700.013.9690369
ATGCGTT500.0015010513.29852211
ACGCAGT1750.013.02712310
AAAAAGT7750.013.0182661
Produced by FastQC (version 0.11.5)