FastQCFastQC Report
Wed 25 May 2016
SRR1295012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822698
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC174540.3619136010589923No Hit
CCCATGTACTCTGCGTTGATACCAC159760.3312668551918449No Hit
GAGTACATGGGAAGCAGTGGTATCA82000.17002930724669055No Hit
GTATCAACGCAGAGTACTTTTTTTT61060.1266096280546698No Hit
CATGTACTCTGCGTTGATACCACTG60960.12640227524095435No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN55730.11555772308363492No Hit
GCGTTGATACCACTGCTTCCCATGT52190.10821743347810707No Hit
GTATCAACGCAGAGTACATGGGAAG49480.10259817222641766No Hit
GGTATCAACGCAGAGTACTTTTTTT49440.10251523110093147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAA752.080847E-411.39686511
AATACCG855.2990163E-511.1824595
AGCTCGA600.00588209211.08247310
TATTCCG2100.010.8629615
TGGACAG7400.09.6335375
CCTAGAC5350.09.5927263
GTCCTAG5450.09.2484841
TAGACTG6950.09.0264175
GTGTAGG6600.08.9338561
GTCTTGC8850.08.9192011
GTCCAGA5900.08.8654721
CTAGACA6150.08.8095044
TGGACCG1201.7549889E-48.7129995
CGGTGCA1107.155454E-48.6356039
GTCTACG1000.0028785038.5592091
GTCTTAT6400.08.4700511
GTCTAGC3150.08.453541
ACCGTCT1356.810148E-58.4437898
GTCCTAC3750.08.3690051
GTCTAGG5350.08.3547841
Produced by FastQC (version 0.11.5)