FastQCFastQC Report
Wed 25 May 2016
SRR1295012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822698
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC177460.3679683032194842No Hit
GTACATGGGAAGCAGTGGTATCAAC172740.35818125041211374No Hit
GTATCAACGCAGAGTACTTTTTTTT157760.32711979891753534No Hit
GGTATCAACGCAGAGTACTTTTTTT135250.2804446805501817No Hit
TATCAACGCAGAGTACTTTTTTTTT106430.22068559963738138No Hit
GAGTACATGGGAAGCAGTGGTATCA88770.18406709273522828No Hit
CATGTACTCTGCGTTGATACCACTG67110.13915447328445613No Hit
ACGCAGAGTACTTTTTTTTTTTTTT60400.1252410994841477No Hit
GCGTTGATACCACTGCTTCCCATGT56530.11721654559335873No Hit
ACGCAGAGTACATGGGAAGCAGTGG55740.11557845836500646No Hit
GCTTCCCATGTACTCTGCGTTGATA52200.10823816875947861No Hit
GTATCAACGCAGAGTACATGGGAAG50980.1057084644321498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA400.0052834714.2490917
AACGCCG400.00528383314.2489436
AACCGTT1001.9336458E-611.3992727
GAACCGT803.776362E-410.68670756
TCTGGAC5400.09.8511213
GTCCTAA5100.09.6984261
GTCCTAC3950.09.6323221
ACCGTCC1106.847485E-59.4993938
CTGCCGC1002.7593065E-49.49939259
CTAGGAC5450.09.4121463
CTAGTAC3950.09.379053
CTGTCGC3900.09.2558189
TAGGACT6350.08.9757134
TCTGTCG3500.08.9565728
CCGTTTG850.00744338478.9406069
TACACTG12450.08.7744485
GTGTAGG5800.08.6919251
CCGTTCA1107.153735E-48.6358139
TAGACTG8800.08.6357235
GTCTAGA6500.08.6338951