FastQCFastQC Report
Wed 25 May 2016
SRR1295011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753538
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC743279.86373613540392No Hit
CCCATGTACTCTGCGTTGATACCAC533997.0864375784631966No Hit
GCTTCCCATGTACTCTGCGTTGATA486806.460191788602565No Hit
GAGTACATGGGAAGCAGTGGTATCA351484.664396486972124No Hit
GCGTTGATACCACTGCTTCCCATGT217092.8809429650528573No Hit
CATGTACTCTGCGTTGATACCACTG175992.3355159262041196No Hit
GTATCAACGCAGAGTACATGGGAAG174742.3189275126138296No Hit
ACGCAGAGTACATGGGAAGCAGTGG171852.280575100393079No Hit
TATCAACGCAGAGTACATGGGAAGC151822.014762361022271No Hit
GGTATCAACGCAGAGTACATGGGAA129751.7218773306721094No Hit
ACTCTGCGTTGATACCACTGCTTCC117781.5630266821314915No Hit
CTGCTTCCCATGTACTCTGCGTTGA91301.2116177286347867No Hit
GTGGTATCAACGCAGAGTACATGGG82491.0947025896504223No Hit
GGGAAGCAGTGGTATCAACGCAGAG80031.0620565917047315No Hit
CAGTGGTATCAACGCAGAGTACATG78781.0454681781144415No Hit
CATGGGAAGCAGTGGTATCAACGCA77111.023306057557814No Hit
CCACTGCTTCCCATGTACTCTGCGT74830.9930487911691248No Hit
GATACCACTGCTTCCCATGTACTCT69140.9175383325061244No Hit
GCAGTGGTATCAACGCAGAGTACAT68150.9044003089426147No Hit
ACATGGGAAGCAGTGGTATCAACGC63630.8444166054001258No Hit
ATACCACTGCTTCCCATGTACTCTG59170.7852291457099708No Hit
GTACTCTGCGTTGATACCACTGCTT57960.76917156135457No Hit
ATGGGAAGCAGTGGTATCAACGCAG57140.7582895620393397No Hit
AAGCAGTGGTATCAACGCAGAGTAC55090.7310845637512641No Hit
CTTCCCATGTACTCTGCGTTGATAC52870.7016235412149088No Hit
GCAGAGTACATGGGAAGCAGTGGTA51870.6883528103426768No Hit
GTTGATACCACTGCTTCCCATGTAC46400.6157619124715675No Hit
TACCACTGCTTCCCATGTACTCTGC45760.607268644713339No Hit
GCTTTCCATGTACTCTGCGTTGATA37230.49406931037319946No Hit
CCATGTACTCTGCGTTGATACCACT35290.4683240924810693No Hit
TTCCCATGTACTCTGCGTTGATACC33610.44602926461571946No Hit
AAAAAGTACTCTGCGTTGATACCAC32750.43461643606559985No Hit
TGGGAAGCAGTGGTATCAACGCAGA32120.4262558756160936No Hit
GGAAGCAGTGGTATCAACGCAGAGT31650.42001863210614465No Hit
ATGGAAAGCAGTGGTATCAACGCAG30900.41006558395197057No Hit
ATCAACGCAGAGTACATGGGAAGCA29950.3974583896233501No Hit
GTACATGGTAAGCAGTGGTATCAAC28840.3827278783551725No Hit
GCTTACCATGTACTCTGCGTTGATA28830.3825951710464502No Hit
TGATACCACTGCTTCCCATGTACTC27010.35844244085898785No Hit
TCCCATGTACTCTGCGTTGATACCA23400.31053510241023013No Hit
ATGGTAAGCAGTGGTATCAACGCAG22030.2923542011152722No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20730.2751022509813706No Hit
CATGGAAAGCAGTGGTATCAACGCA20150.2674052270754759No Hit
TCCATGTACTCTGCGTTGATACCAC18650.24749913076712787No Hit
ACCATGTACTCTGCGTTGATACCAC18510.24564122844501537No Hit
CTGCGTTGATACCACTGCTTCCCAT18140.2407310580222895No Hit
GTACATGGAAAGCAGTGGTATCAAC17930.2379442045391208No Hit
GTATAACGCAGAGTACATGGGAAGC17080.22666408329772353No Hit
ACTGCTTCCCATGTACTCTGCGTTG15780.20941213316382187No Hit
CTCTGCGTTGATACCACTGCTTCCC13700.18180901294957919No Hit
AAAGTACTCTGCGTTGATACCACTG13110.17397928173496227No Hit
AGTGGTATCAACGCAGAGTACATGG12810.16999806247329263No Hit
GGTAAGCAGTGGTATCAACGCAGAG12790.16973264785584802No Hit
GTATCAACGCAGAGTACTTTTTTTT12740.16906911131223643No Hit
GCTTACCCATGTACTCTGCGTTGAT12590.1670785016814016No Hit
GTACATGGGTAAGCAGTGGTATCAA12560.16668037975523464No Hit
GAGTACATGGAAAGCAGTGGTATCA12380.1642916481982329No Hit
TCAACGCAGAGTACATGGGAAGCAG12160.16137208740634182No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA11310.15009196616494455No Hit
GGTATCAACGCAGAGTACTTTTTTT11120.14757052729922046No Hit
GAGTACATGGTAAGCAGTGGTATCA10620.14093516186310445No Hit
TATCAACGCAGAGTACTTTTTTTTT9610.12753172368215007No Hit
GCGTTGATACCACTGCTTACCATGT9300.12341779711175813No Hit
AACGCAGAGTACATGGGAAGCAGTG9170.12169260209836798No Hit
GTACATGGAAGCAGTGGTATCAACG9040.1199674070849778No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN8850.11744596821925371No Hit
TTGATACCACTGCTTCCCATGTACT8700.1154553585884189No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA8670.11505723666225193No Hit
GCGTTGATACCACTGCTTTCCATGT8640.11465911473608498No Hit
GTACTTGGGAAGCAGTGGTATCAAC7970.10576772505168949No Hit
CCCCATGTACTCTGCGTTGATACCA7710.10231733502490917No Hit
CATGGTAAGCAGTGGTATCAACGCA7650.10152109117257523No Hit
CAACGCAGAGTACATGGGAAGCAGT7560.10032672539407436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAG307.696599E-419.0077194
GCACTGC307.7242055E-418.99760610
TACAAGA1000.018.0573352
ACGGGAA504.538384E-617.1069495
CACGGGA504.538384E-617.1069494
CGGGAAG504.561456E-617.0978456
GTACACG702.4620022E-816.3042681
TGATAGC350.002162919416.2923325
TACACGG651.9006256E-716.0834562
TACATGA2550.016.0261172
TACATAA1850.015.9253872
ATAACGC2450.015.9044193
TATAACG2350.015.7723652
GGAAGCG551.1281096E-515.5434948
GTACATA2350.015.3792031
GTATAAC2550.015.2919121
GAGCAAA508.620257E-515.2173181
GTACAAG1650.014.9867521
GTACAAA3050.014.9678541
GTACAAC704.370395E-714.945581
Produced by FastQC (version 0.11.5)