##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295011_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 753538 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.53573409701966 33.0 33.0 33.0 22.0 33.0 2 30.143286735373664 33.0 33.0 33.0 22.0 33.0 3 30.493231927255163 33.0 33.0 33.0 27.0 33.0 4 33.497732032093936 37.0 33.0 37.0 22.0 37.0 5 34.2561954407077 37.0 33.0 37.0 27.0 37.0 6 33.965721702157026 37.0 37.0 37.0 27.0 37.0 7 33.973394838747346 37.0 37.0 37.0 27.0 37.0 8 33.89114550294743 37.0 37.0 37.0 27.0 37.0 9 33.66315301948939 37.0 37.0 37.0 22.0 37.0 10 33.77842391491869 37.0 33.0 37.0 27.0 37.0 11 33.88767255267817 37.0 37.0 37.0 27.0 37.0 12 33.98625152281637 37.0 37.0 37.0 27.0 37.0 13 33.602032014311156 37.0 33.0 37.0 22.0 37.0 14 35.02091599892773 37.0 33.0 40.0 22.0 40.0 15 34.940225974005294 37.0 33.0 40.0 22.0 40.0 16 34.875176832488876 37.0 33.0 40.0 27.0 40.0 17 35.02874573014234 37.0 33.0 40.0 27.0 40.0 18 34.90353638436283 37.0 33.0 40.0 22.0 40.0 19 34.72585324164143 37.0 33.0 40.0 22.0 40.0 20 34.88105842041145 37.0 33.0 40.0 22.0 40.0 21 34.83228583030982 37.0 33.0 40.0 22.0 40.0 22 34.97533501960087 37.0 33.0 40.0 22.0 40.0 23 35.0179061971659 37.0 33.0 40.0 22.0 40.0 24 35.22777484347173 37.0 33.0 40.0 27.0 40.0 25 34.1270898083441 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5996429412627684 1101 2 0.638194788122707 1101 3 0.479733654812307 1101 4 0.569375573936 1101 5 0.3067895748839575 1101 6 0.14983478018105245 1101 7 0.5096077063423863 1101 8 0.265028274566113 1101 9 0.08121224960991924 1101 10 0.39822206136666694 1101 11 0.16568512053323303 1101 12 0.4210211586398884 1101 13 0.3405932801142626 1101 14 0.77006770629675 1101 15 0.8513943029433264 1101 16 0.7989494113259141 1101 17 0.6084944079490171 1101 18 0.7715547019725193 1101 19 0.9119752492029747 1101 20 0.4604179504121291 1101 21 0.488121970954694 1101 22 0.7089454506004742 1101 23 0.712004469457618 1101 24 0.8289984989301615 1101 25 1.2065662979854181 1102 1 0.6141341132256244 1102 2 0.5557167376139667 1102 3 0.5161885529771055 1102 4 0.7126732091956356 1102 5 0.30607308275350675 1102 6 0.23705385753531516 1102 7 0.3442625168832052 1102 8 0.4001186743623961 1102 9 0.3252964445527198 1102 10 0.5756622073855482 1102 11 0.4936542528501917 1102 12 0.5114510064683486 1102 13 0.6682444987044676 1102 14 1.0094108743940282 1102 15 0.8193981052547628 1102 16 0.8775452765494691 1102 17 0.7554883834048169 1102 18 0.8519172817870526 1102 19 0.7359245917858601 1102 20 0.7320135451717746 1102 21 0.6887524207630804 1102 22 0.5646205269551245 1102 23 0.7315949143312253 1102 24 0.8596392137140967 1102 25 0.9874707546297401 1103 1 0.7088715566261996 1103 2 0.8785434910061234 1103 3 0.6898007483684161 1103 4 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-0.014256844868441476 2214 19 -0.7760157721213758 2214 20 -0.400330052492734 2214 21 -0.16292279038417945 2214 22 -0.3567688822707211 2214 23 -1.8084203671436896 2214 24 -1.3525473517333353 2214 25 -1.4329704089532456 2215 1 -0.6300343097964429 2215 2 -0.34669296570566743 2215 3 -1.4970380423509297 2215 4 -1.0624153299657948 2215 5 -0.34361412425394633 2215 6 -0.14834159644212264 2215 7 -0.22198320625468426 2215 8 -0.19637452559264545 2215 9 -0.5952078499369406 2215 10 -1.613220638880584 2215 11 -0.5552337455469285 2215 12 -0.6263035719819214 2215 13 -0.7940761802598288 2215 14 -0.8750585224366461 2215 15 -0.8197146670734057 2215 16 -0.77303273250795 2215 17 -0.616892005825413 2215 18 -0.6322650477999332 2215 19 -1.431526646226537 2215 20 -1.6630836925739416 2215 21 -0.8284056414492511 2215 22 -0.6009639362284318 2215 23 -0.5923721985974879 2215 24 -1.7704852542306426 2215 25 -0.9816604411044665 2216 1 -0.5011100273121691 2216 2 -0.6949012549189781 2216 3 -1.514311255840969 2216 4 -0.7079156636103363 2216 5 -0.8759337348508183 2216 6 -0.5536567303714364 2216 7 -0.3196737525607318 2216 8 -0.32658371748180315 2216 9 -0.23377471165549935 2216 10 -0.3209501787678448 2216 11 -0.5660651359196791 2216 12 -0.6173171243569158 2216 13 -1.315879689178253 2216 14 -1.0581944644120043 2216 15 -1.6952238619139024 2216 16 -1.2478481482535244 2216 17 -1.8312501665837786 2216 18 -1.1141708599814493 2216 19 -0.8631640671074265 2216 20 -0.8377629871046892 2216 21 -0.38382770719486103 2216 22 -0.34394174436734204 2216 23 -0.6038737574049478 2216 24 -0.719296191149148 2216 25 -0.8546048559955963 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1005.0 3 113.0 4 148.0 5 93.0 6 70.0 7 73.0 8 101.0 9 172.0 10 398.0 11 766.0 12 1489.0 13 2603.0 14 4462.0 15 7339.0 16 9776.0 17 10495.0 18 9550.0 19 8419.0 20 7607.0 21 7339.0 22 6862.0 23 6462.0 24 6168.0 25 5911.0 26 5833.0 27 6480.0 28 7186.0 29 8326.0 30 10098.0 31 12936.0 32 17696.0 33 25784.0 34 37741.0 35 61418.0 36 113570.0 37 349049.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.54418078343928 15.25210503544447 8.072686125070723 21.13102805604553 2 9.813616535865421 24.246336213486778 31.954945696322184 33.98510155432562 3 26.19513674093026 32.69495190197951 26.92106180003883 14.188849557051405 4 14.060496592378206 21.163166827160953 30.0748559541607 34.70148062630013 5 9.220301640181793 43.61063510478644 29.43932072419188 17.729742530839886 6 29.820966493714412 24.176171948757908 20.472369747273408 25.53049181025427 7 20.71903168201769 35.39950562915272 18.68463846971081 25.196824219118774 8 29.252641588477417 36.60573336081871 20.515350804279738 13.626274246424131 9 29.927648991208823 19.884956576094908 21.19778159039221 28.98961284230406 10 23.790434134922325 36.047962332411146 23.472281336454564 16.689322196211958 11 21.806042037345843 32.40048510767316 17.772121734366316 28.021351120614685 12 26.692634221851048 42.031666409562156 20.976935066040394 10.298764302546399 13 27.107647741827368 22.42744414253015 16.602094247801148 33.86281386784134 14 21.77100178738616 33.169977232104856 26.23374861690357 18.825272363605414 15 39.41614672262382 17.490465476247465 16.514938750325797 26.57844905080292 16 12.040101757078247 27.383186145505174 41.89114971402431 18.685562383392266 17 35.56993519852019 29.463196935061546 22.700199072060894 12.266668794357365 18 28.58351142561151 20.45558819657132 27.44859704954179 23.51230332827538 19 29.444728880614385 31.120582466172415 28.02805944346554 11.406629209747665 20 24.234256277143775 27.479009699287516 27.8953583310949 20.391375692473808 21 15.570277210054401 29.071751222359776 37.56823207615664 17.789739491429188 22 26.792280206226422 29.363727394948597 12.903380235615147 30.940612163209835 23 12.771523029089234 37.86481231764371 25.33346099935901 24.030203653908046 24 20.330680300700678 41.54904474864466 21.365735445118933 16.754539505535725 25 20.290678111496653 29.555244933814073 14.82064697691306 35.33342997777621 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2379.0 1 2379.0 2 2933.5 3 3488.0 4 3488.0 5 3488.0 6 4879.5 7 6271.0 8 6271.0 9 6271.0 10 6352.5 11 6434.0 12 6434.0 13 6434.0 14 6880.5 15 7327.0 16 7327.0 17 7327.0 18 8169.0 19 9011.0 20 9011.0 21 9011.0 22 9550.0 23 10089.0 24 10089.0 25 10089.0 26 10938.5 27 11788.0 28 11788.0 29 11788.0 30 11993.5 31 12199.0 32 12199.0 33 12199.0 34 12383.5 35 12568.0 36 12568.0 37 12568.0 38 14426.0 39 16284.0 40 16284.0 41 16284.0 42 31491.5 43 46699.0 44 46699.0 45 46699.0 46 197378.5 47 348058.0 48 348058.0 49 348058.0 50 269900.0 51 191742.0 52 191742.0 53 191742.0 54 122534.5 55 53327.0 56 53327.0 57 53327.0 58 30898.0 59 8469.0 60 8469.0 61 8469.0 62 6380.5 63 4292.0 64 4292.0 65 4292.0 66 3118.5 67 1945.0 68 1945.0 69 1945.0 70 1318.5 71 692.0 72 692.0 73 692.0 74 477.0 75 262.0 76 262.0 77 262.0 78 193.0 79 124.0 80 124.0 81 124.0 82 87.0 83 50.0 84 50.0 85 50.0 86 38.5 87 27.0 88 27.0 89 27.0 90 18.0 91 9.0 92 9.0 93 9.0 94 5.5 95 2.0 96 2.0 97 2.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3135873705108435 2 0.18300337872808006 3 0.20330759696259512 4 0.22480618097561106 5 0.2540017888945216 6 0.19906096308348084 7 0.19521245113053357 8 0.2153839620563263 9 0.21870164477438428 10 0.22533701021050034 11 0.2043692554323737 12 0.2088813039289326 13 0.22148849825755304 14 0.21259710857315756 15 0.20357301158003974 16 0.2058290358283192 17 0.20556362121087454 18 0.21060649894232272 19 0.20742152353298704 20 0.20224593849281655 21 0.20025532886198175 22 0.20556362121087454 23 0.20914671854637723 24 0.22480618097561106 25 0.21777269361332807 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 753538.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.945193486164335 #Duplication Level Percentage of deduplicated Percentage of total 1 90.40270845080963 15.318913863722727 2 4.587108658283007 1.5545888751333048 3 1.4113854777007149 0.7174860000960328 4 0.758102857164282 0.5138479838825105 5 0.45552796724342504 0.3859504771649483 6 0.32497450152022717 0.33040534837980334 7 0.2519405352316609 0.29884267815558074 8 0.19307008216922064 0.2617287918997673 9 0.15932092695439978 0.2429751540273623 >10 1.0948385411233683 3.7696310635894514 >50 0.1640295616874282 1.9429990414434333 >100 0.12243354843654926 4.308909578560385 >500 0.026684234915658172 3.130077032129544 >1k 0.027469065354354005 10.381679272566648 >5k 0.01177245658043743 13.55923410468218 >10k+ 0.008633134825654115 43.28273073456632 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 74327 9.86373613540392 No Hit CCCATGTACTCTGCGTTGATACCAC 53399 7.0864375784631966 No Hit GCTTCCCATGTACTCTGCGTTGATA 48680 6.460191788602565 No Hit GAGTACATGGGAAGCAGTGGTATCA 35148 4.664396486972124 No Hit GCGTTGATACCACTGCTTCCCATGT 21709 2.8809429650528573 No Hit CATGTACTCTGCGTTGATACCACTG 17599 2.3355159262041196 No Hit GTATCAACGCAGAGTACATGGGAAG 17474 2.3189275126138296 No Hit ACGCAGAGTACATGGGAAGCAGTGG 17185 2.280575100393079 No Hit TATCAACGCAGAGTACATGGGAAGC 15182 2.014762361022271 No Hit GGTATCAACGCAGAGTACATGGGAA 12975 1.7218773306721094 No Hit ACTCTGCGTTGATACCACTGCTTCC 11778 1.5630266821314915 No Hit CTGCTTCCCATGTACTCTGCGTTGA 9130 1.2116177286347867 No Hit GTGGTATCAACGCAGAGTACATGGG 8249 1.0947025896504223 No Hit GGGAAGCAGTGGTATCAACGCAGAG 8003 1.0620565917047315 No Hit CAGTGGTATCAACGCAGAGTACATG 7878 1.0454681781144415 No Hit CATGGGAAGCAGTGGTATCAACGCA 7711 1.023306057557814 No Hit CCACTGCTTCCCATGTACTCTGCGT 7483 0.9930487911691248 No Hit GATACCACTGCTTCCCATGTACTCT 6914 0.9175383325061244 No Hit GCAGTGGTATCAACGCAGAGTACAT 6815 0.9044003089426147 No Hit ACATGGGAAGCAGTGGTATCAACGC 6363 0.8444166054001258 No Hit ATACCACTGCTTCCCATGTACTCTG 5917 0.7852291457099708 No Hit GTACTCTGCGTTGATACCACTGCTT 5796 0.76917156135457 No Hit ATGGGAAGCAGTGGTATCAACGCAG 5714 0.7582895620393397 No Hit AAGCAGTGGTATCAACGCAGAGTAC 5509 0.7310845637512641 No Hit CTTCCCATGTACTCTGCGTTGATAC 5287 0.7016235412149088 No Hit GCAGAGTACATGGGAAGCAGTGGTA 5187 0.6883528103426768 No Hit GTTGATACCACTGCTTCCCATGTAC 4640 0.6157619124715675 No Hit TACCACTGCTTCCCATGTACTCTGC 4576 0.607268644713339 No Hit GCTTTCCATGTACTCTGCGTTGATA 3723 0.49406931037319946 No Hit CCATGTACTCTGCGTTGATACCACT 3529 0.4683240924810693 No Hit TTCCCATGTACTCTGCGTTGATACC 3361 0.44602926461571946 No Hit AAAAAGTACTCTGCGTTGATACCAC 3275 0.43461643606559985 No Hit TGGGAAGCAGTGGTATCAACGCAGA 3212 0.4262558756160936 No Hit GGAAGCAGTGGTATCAACGCAGAGT 3165 0.42001863210614465 No Hit ATGGAAAGCAGTGGTATCAACGCAG 3090 0.41006558395197057 No Hit ATCAACGCAGAGTACATGGGAAGCA 2995 0.3974583896233501 No Hit GTACATGGTAAGCAGTGGTATCAAC 2884 0.3827278783551725 No Hit GCTTACCATGTACTCTGCGTTGATA 2883 0.3825951710464502 No Hit TGATACCACTGCTTCCCATGTACTC 2701 0.35844244085898785 No Hit TCCCATGTACTCTGCGTTGATACCA 2340 0.31053510241023013 No Hit ATGGTAAGCAGTGGTATCAACGCAG 2203 0.2923542011152722 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2073 0.2751022509813706 No Hit CATGGAAAGCAGTGGTATCAACGCA 2015 0.2674052270754759 No Hit TCCATGTACTCTGCGTTGATACCAC 1865 0.24749913076712787 No Hit ACCATGTACTCTGCGTTGATACCAC 1851 0.24564122844501537 No Hit CTGCGTTGATACCACTGCTTCCCAT 1814 0.2407310580222895 No Hit GTACATGGAAAGCAGTGGTATCAAC 1793 0.2379442045391208 No Hit GTATAACGCAGAGTACATGGGAAGC 1708 0.22666408329772353 No Hit ACTGCTTCCCATGTACTCTGCGTTG 1578 0.20941213316382187 No Hit CTCTGCGTTGATACCACTGCTTCCC 1370 0.18180901294957919 No Hit AAAGTACTCTGCGTTGATACCACTG 1311 0.17397928173496227 No Hit AGTGGTATCAACGCAGAGTACATGG 1281 0.16999806247329263 No Hit GGTAAGCAGTGGTATCAACGCAGAG 1279 0.16973264785584802 No Hit GTATCAACGCAGAGTACTTTTTTTT 1274 0.16906911131223643 No Hit GCTTACCCATGTACTCTGCGTTGAT 1259 0.1670785016814016 No Hit GTACATGGGTAAGCAGTGGTATCAA 1256 0.16668037975523464 No Hit GAGTACATGGAAAGCAGTGGTATCA 1238 0.1642916481982329 No Hit TCAACGCAGAGTACATGGGAAGCAG 1216 0.16137208740634182 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 1131 0.15009196616494455 No Hit GGTATCAACGCAGAGTACTTTTTTT 1112 0.14757052729922046 No Hit GAGTACATGGTAAGCAGTGGTATCA 1062 0.14093516186310445 No Hit TATCAACGCAGAGTACTTTTTTTTT 961 0.12753172368215007 No Hit GCGTTGATACCACTGCTTACCATGT 930 0.12341779711175813 No Hit AACGCAGAGTACATGGGAAGCAGTG 917 0.12169260209836798 No Hit GTACATGGAAGCAGTGGTATCAACG 904 0.1199674070849778 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 885 0.11744596821925371 No Hit TTGATACCACTGCTTCCCATGTACT 870 0.1154553585884189 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 867 0.11505723666225193 No Hit GCGTTGATACCACTGCTTTCCATGT 864 0.11465911473608498 No Hit GTACTTGGGAAGCAGTGGTATCAAC 797 0.10576772505168949 No Hit CCCCATGTACTCTGCGTTGATACCA 771 0.10231733502490917 No Hit CATGGTAAGCAGTGGTATCAACGCA 765 0.10152109117257523 No Hit CAACGCAGAGTACATGGGAAGCAGT 756 0.10032672539407436 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.3270730872232058E-4 0.0 12 0.0 0.0 0.0 1.3270730872232058E-4 0.0 13 0.0 0.0 0.0 1.3270730872232058E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGATAG 30 7.696599E-4 19.007719 4 GCACTGC 30 7.7242055E-4 18.997606 10 TACAAGA 100 0.0 18.057335 2 ACGGGAA 50 4.538384E-6 17.106949 5 CACGGGA 50 4.538384E-6 17.106949 4 CGGGAAG 50 4.561456E-6 17.097845 6 GTACACG 70 2.4620022E-8 16.304268 1 TGATAGC 35 0.0021629194 16.292332 5 TACACGG 65 1.9006256E-7 16.083456 2 TACATGA 255 0.0 16.026117 2 TACATAA 185 0.0 15.925387 2 ATAACGC 245 0.0 15.904419 3 TATAACG 235 0.0 15.772365 2 GGAAGCG 55 1.1281096E-5 15.543494 8 GTACATA 235 0.0 15.379203 1 GTATAAC 255 0.0 15.291912 1 GAGCAAA 50 8.620257E-5 15.217318 1 GTACAAG 165 0.0 14.986752 1 GTACAAA 305 0.0 14.967854 1 GTACAAC 70 4.370395E-7 14.94558 1 >>END_MODULE