FastQCFastQC Report
Wed 25 May 2016
SRR1295011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753538
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC736949.779732408982692No Hit
CCCATGTACTCTGCGTTGATACCAC585597.771207291470371No Hit
GCTTCCCATGTACTCTGCGTTGATA539927.165133012535533No Hit
GAGTACATGGGAAGCAGTGGTATCA380235.045929999548795No Hit
GCGTTGATACCACTGCTTCCCATGT228083.0267882973386877No Hit
ACGCAGAGTACATGGGAAGCAGTGG206762.7438563151427No Hit
CATGTACTCTGCGTTGATACCACTG198402.63291300505084No Hit
GTATCAACGCAGAGTACATGGGAAG185722.464640137590938No Hit
TATCAACGCAGAGTACATGGGAAGC165582.197367617824184No Hit
GGTATCAACGCAGAGTACATGGGAA140381.8629451998439361No Hit
ACTCTGCGTTGATACCACTGCTTCC127881.6970610639410355No Hit
CATGGGAAGCAGTGGTATCAACGCA97681.2962849915996273No Hit
CTGCTTCCCATGTACTCTGCGTTGA97391.29243647964668No Hit
GGGAAGCAGTGGTATCAACGCAGAG94121.249041189694481No Hit
CAGTGGTATCAACGCAGAGTACATG92911.2329836053390804No Hit
GTGGTATCAACGCAGAGTACATGGG90351.1990105343061663No Hit
CCACTGCTTCCCATGTACTCTGCGT83661.110229344770934No Hit
ACATGGGAAGCAGTGGTATCAACGC81541.082095395321802No Hit
GATACCACTGCTTCCCATGTACTCT77091.0230406429403693No Hit
GCAGTGGTATCAACGCAGAGTACAT76521.015476326343197No Hit
GTACTTTTTTTTTTTTTTTTTTTTT71710.9516441108477608No Hit
ATGGGAAGCAGTGGTATCAACGCAG68480.9087796501304513No Hit
AAGCAGTGGTATCAACGCAGAGTAC62680.8318094110715053No Hit
ATACCACTGCTTCCCATGTACTCTG59840.7941205353943662No Hit
GCAGAGTACATGGGAAGCAGTGGTA58340.7742144390860182No Hit
GTACTCTGCGTTGATACCACTGCTT55330.7342695391605998No Hit
CTTCCCATGTACTCTGCGTTGATAC55180.7322789295297649No Hit
TACCACTGCTTCCCATGTACTCTGC48550.6442939838468664No Hit
GTTGATACCACTGCTTCCCATGTAC45080.5982445477202212No Hit
GGAAGCAGTGGTATCAACGCAGAGT42030.5577688185599134No Hit
GCTTTCCATGTACTCTGCGTTGATA41680.5531240627546321No Hit
CCATGTACTCTGCGTTGATACCACT41090.5452943315400152No Hit
GTATCAACGCAGAGTACTTTTTTTT40750.5407822830434563No Hit
ATGGAAAGCAGTGGTATCAACGCAG40160.5329525518288394No Hit
TGGGAAGCAGTGGTATCAACGCAGA39970.5304311129631153No Hit
GGTATCAACGCAGAGTACTTTTTTT38920.5164968455472717No Hit
ATCAACGCAGAGTACATGGGAAGCA34990.4643428732193997No Hit
TATCAACGCAGAGTACTTTTTTTTT34780.4615560197362309No Hit
AAAAAGTACTCTGCGTTGATACCAC34050.45186838619950154No Hit
TTCCCATGTACTCTGCGTTGATACC32780.4350145579917668No Hit
GCTTACCATGTACTCTGCGTTGATA31840.42254007097186874No Hit
GTACATGGTAAGCAGTGGTATCAAC28840.3827278783551725No Hit
ATGGTAAGCAGTGGTATCAACGCAG27390.36348531859043604No Hit
TGATACCACTGCTTCCCATGTACTC27090.3595040993287664No Hit
CATGGAAAGCAGTGGTATCAACGCA27050.35897327009387714No Hit
TCCCATGTACTCTGCGTTGATACCA25360.336545734919805No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23940.31770129708123546No Hit
ACCATGTACTCTGCGTTGATACCAC21010.27881805562559553No Hit
CTGCGTTGATACCACTGCTTCCCAT20480.27178456826331254No Hit
TCCATGTACTCTGCGTTGATACCAC20250.26873230016269917No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19790.2626277639614724No Hit
GTACATGGAAAGCAGTGGTATCAAC19400.2574521789213019No Hit
ACTGCTTCCCATGTACTCTGCGTTG18470.2451103992101261No Hit
GTATAACGCAGAGTACATGGGAAGC17930.2379442045391208No Hit
GGTAAGCAGTGGTATCAACGCAGAG15850.2103410843248781No Hit
CTCTGCGTTGATACCACTGCTTCCC15210.2018478165666496No Hit
AGTGGTATCAACGCAGAGTACATGG14200.18844437838569522No Hit
GTACATGGGTAAGCAGTGGTATCAA14160.18791354915080594No Hit
GCTTACCCATGTACTCTGCGTTGAT14010.18592293951997113No Hit
AAAGTACTCTGCGTTGATACCACTG13980.18552481759380415No Hit
TCAACGCAGAGTACATGGGAAGCAG13420.17809320830535422No Hit
GAGTACATGGAAAGCAGTGGTATCA13300.17650072060068636No Hit
GAGTACATGGTAAGCAGTGGTATCA12000.1592487704667847No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11130.1477032346079428No Hit
AACGCAGAGTACATGGGAAGCAGTG10820.14358930803755088No Hit
GTGGTATCAACGCAGAGTACTTTTT10560.14013891801077052No Hit
GTACATGGAAGCAGTGGTATCAACG9730.12912421138681793No Hit
CATGGTAAGCAGTGGTATCAACGCA9690.12859338215192864No Hit
GCGTTGATACCACTGCTTTCCATGT9220.12235613864197957No Hit
CAACGCAGAGTACATGGGAAGCAGT9110.12089635824603404No Hit
GCGTTGATACCACTGCTTACCATGT9080.12049823631986709No Hit
TTGATACCACTGCTTCCCATGTACT8980.11917116323264389No Hit
CCCCATGTACTCTGCGTTGATACCA8690.11532265127969657No Hit
GTACTTGGGAAGCAGTGGTATCAAC8650.1147918220448073No Hit
CGCAGAGTACATGGGAAGCAGTGGT8260.1096162370046368No Hit
CACTGCTTCCCATGTACTCTGCGTT7980.10590043236041183No Hit
ACGCAGAGTACATGGAAAGCAGTGG7840.10404253003829932No Hit
CGTTGATACCACTGCTTCCCATGTA7610.10099026193768595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAAGA401.2848186E-518.9982092
GTACAAG1000.018.080691
GTACAAA1250.017.5097221
TACAAGG504.5601064E-617.0983892
TACATAA453.5378456E-516.8872972
CATTGGA402.723219E-416.6532691
ATTGGAA402.7607338E-416.6234322
TTGGAAG402.7607338E-416.6234323
GTATCGA350.002170171616.28417818
GTACCTG601.4386496E-615.8602551
ACGTGGG551.1277771E-515.5439893
GTATAAC2650.015.4413061
TATAACG2600.015.34470752
GGACGCA508.711453E-515.1985678
AAAAAGT4650.015.1439871
ATAACGC2800.014.9271643
TACAAAA1350.014.7763852
AAAAGTA4800.014.6444522
CTGGGAA653.3698507E-614.6140075
ACGGGAA653.3698507E-614.6140075
Produced by FastQC (version 0.11.5)