FastQCFastQC Report
Wed 25 May 2016
SRR1295010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634200
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC34520.544307789340902No Hit
CCCATGTACTCTGCGTTGATACCAC33900.5345316934720908No Hit
GAGTACATGGGAAGCAGTGGTATCA13630.21491643014821823No Hit
CATGTACTCTGCGTTGATACCACTG11250.1773888363292337No Hit
GCGTTGATACCACTGCTTCCCATGT9700.15294859665720592No Hit
GCTTCCCATGTACTCTGCGTTGATA8560.13497319457584359No Hit
ACGCAGAGTACATGGGAAGCAGTGG8430.13292336802270577No Hit
GTATCAACGCAGAGTACATGGGAAG7390.1165247555976033No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN7100.1119520655944497No Hit
ACTCTGCGTTGATACCACTGCTTCC6820.10753705455692211No Hit
TATCAACGCAGAGTACATGGGAAGC6770.10674865972879219No Hit
GGTATCAACGCAGAGTACATGGGAA6640.10469883317565437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGT400.00523492414.2661941
GACAGGC655.4491204E-513.1521517
GTCTTAC701.0774427E-412.2281671
GTCTACT550.003039022712.104651
CTAGTAC550.003049843712.0989083
ACTGTTC951.0371568E-611.9994018
GCCCAGA802.8270115E-511.8884951
TTAGGGT657.9517125E-411.7028184
TAATGCT907.3668343E-611.621554
CTAGGAC907.3863193E-611.6187933
AGCACTG855.2626732E-511.1865185
TAGACCC600.005830005311.0932975
GTCTTGG700.001475238110.8694821
CTAGACA803.732888E-410.6971084
AAAGGTG909.407599E-510.5650445
GGTTTAA1105.94461E-610.3754141
CATACTG1105.9614704E-610.3729535
CTAGACT750.002624225610.1424434
GAGGTGT750.002646695110.1320276
AACCCGA750.00265190410.12962611