##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295010_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	634200
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90726900031536	33.0	33.0	33.0	27.0	33.0
2	30.982660044150112	33.0	33.0	33.0	27.0	33.0
3	31.009323557237465	33.0	33.0	33.0	27.0	33.0
4	34.60140491958373	37.0	37.0	37.0	27.0	37.0
5	34.75116051718701	37.0	37.0	37.0	27.0	37.0
6	34.74595711132135	37.0	37.0	37.0	27.0	37.0
7	34.641316619362975	37.0	37.0	37.0	27.0	37.0
8	34.55977136549984	37.0	37.0	37.0	27.0	37.0
9	34.55792179123305	37.0	37.0	37.0	27.0	37.0
10	34.43560864080732	37.0	37.0	37.0	27.0	37.0
11	34.616802270577104	37.0	37.0	37.0	27.0	37.0
12	34.51041627246925	37.0	37.0	37.0	27.0	37.0
13	34.44222958057395	37.0	37.0	37.0	27.0	37.0
14	35.7164490696941	37.0	37.0	40.0	27.0	40.0
15	35.86604856512141	37.0	37.0	40.0	27.0	40.0
16	35.93914853358562	37.0	37.0	40.0	27.0	40.0
17	35.86539577420372	37.0	37.0	40.0	27.0	40.0
18	35.8059697256386	37.0	37.0	40.0	27.0	40.0
19	35.891169977924946	37.0	37.0	40.0	27.0	40.0
20	35.87186534216335	37.0	37.0	40.0	27.0	40.0
21	35.863637653736994	37.0	37.0	40.0	27.0	40.0
22	35.76279091769158	37.0	37.0	40.0	27.0	40.0
23	35.65458688111006	37.0	37.0	40.0	27.0	40.0
24	35.698716493219806	37.0	37.0	40.0	27.0	40.0
25	34.39620466729738	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6349759267951463
1101	2	0.5729440210243055
1101	3	0.5270833516742961
1101	4	0.7064836603143334
1101	5	0.6227458761029396
1101	6	0.5453434030779434
1101	7	0.7368276805732492
1101	8	0.516423632937844
1101	9	0.64342579242863
1101	10	0.8179862943549168
1101	11	0.6716591322044465
1101	12	0.6282800911023259
1101	13	0.6449572581677927
1101	14	1.1085602879693823
1101	15	0.9829864811824081
1101	16	1.0470523046076323
1101	17	0.9547560563885398
1101	18	0.8894715634119379
1101	19	0.9201053919710063
1101	20	0.9142515543287146
1101	21	1.0114051450489256
1101	22	1.004151603437201
1101	23	1.1087340306752296
1101	24	1.2012824735699397
1101	25	1.384652687644376
1102	1	0.3041918943772295
1102	2	0.41042184160680506
1102	3	0.2977401821356267
1102	4	0.22157465587045522
1102	5	0.17801758395805223
1102	6	0.21167804453067873
1102	7	0.3253825016992451
1102	8	0.2832829874568503
1102	9	0.19671633880159334
1102	10	0.5867079206856829
1102	11	0.4181124235500775
1102	12	0.5815404654749088
1102	13	0.4774936643828269
1102	14	0.9700488266055274
1102	15	0.7919707437867061
1102	16	0.7968884566585288
1102	17	0.887171774602038
1102	18	0.9368186253384749
1102	19	0.7465044936046894
1102	20	0.8554171526534731
1102	21	0.6897656265221173
1102	22	0.9357860466738757
1102	23	0.7882504394478858
1102	24	1.0062964206270735
1102	25	1.050854452406675
1103	1	0.1644339843537459
1103	2	0.3171275949129466
1103	3	0.42317276154484773
1103	4	0.3882529094855869
1103	5	0.15818459050854017
1103	6	0.13892359514247232
1103	7	0.018401751482954865
1103	8	0.14813668292094206
1103	9	0.1416088119198733
1103	10	0.4833562027274567
1103	11	0.25190151018955476
1103	12	0.18923342678995425
1103	13	0.2284785550020345
1103	14	0.5166129101551462
1103	15	0.5942703668246523
1103	16	0.5301878016097135
1103	17	0.36886053371710403
1103	18	0.2806767701907802
1103	19	0.5059980667106103
1103	20	0.27917889409457786
1103	21	0.4599809892708464
1103	22	0.586451137515553
1103	23	0.531271234158055
1103	24	0.5815459262506053
1103	25	0.5460497311114096
1104	1	0.07279033293253079
1104	2	0.18919338986525247
1104	3	-0.04520276322184813
1104	4	-0.15318312723184846
1104	5	-0.008303493133254847
1104	6	0.03165058658194653
1104	7	0.26231464880631705
1104	8	0.19364765956954244
1104	9	0.18182150716746293
1104	10	0.33968186176566917
1104	11	0.2315611901534922
1104	12	0.42347132611878635
1104	13	0.21056963807465934
1104	14	0.5929060613528989
1104	15	0.38654488426848843
1104	16	0.4354767241142312
1104	17	0.3840410458206378
1104	18	0.44068907045473793
1104	19	0.5606969967404467
1104	20	0.41819957152588216
1104	21	0.5596780666211743
1104	22	0.37733137038017617
1104	23	0.5347197010180125
1104	24	0.5254536666309946
1104	25	0.2496009548348752
1105	1	-0.1076761726889437
1105	2	-0.16870524624223293
1105	3	-0.00305351925414854
1105	4	-0.11849371720106916
1105	5	0.014021823407240674
1105	6	-0.14327621404384416
1105	7	-0.24739338125370125
1105	8	0.10226567902895312
1105	9	0.16565360770380266
1105	10	0.43099980516866054
1105	11	0.14617407087890655
1105	12	0.2040820630778697
1105	13	0.22879657780881502
1105	14	0.5518415079774499
1105	15	0.49051164012739434
1105	16	0.16879652754977315
1105	17	0.12245722022233707
1105	18	0.08617553595940564
1105	19	0.47716399889603167
1105	20	0.2796369983757714
1105	21	0.4477106093093113
1105	22	0.24708672881081384
1105	23	0.38429547961109023
1105	24	0.26988736159353977
1105	25	0.23813929239137366
1106	1	0.16747936002166597
1106	2	0.164214514831162
1106	3	0.052242568943093204
1106	4	0.28855444033450794
1106	5	-0.32218583713264337
1106	6	-0.27030788777078385
1106	7	-0.41761529231203554
1106	8	0.09264126172272569
1106	9	0.5478121004101624
1106	10	0.18298938314771362
1106	11	-0.06506239201479502
1106	12	-0.21224931464949037
1106	13	0.1954390209573944
1106	14	0.09351186725358218
1106	15	0.3798441344998551
1106	16	0.3833740634862437
1106	17	0.4852162198501233
1106	18	0.04921103818383443
1106	19	0.054646761795055454
1106	20	0.13394583050678932
1106	21	0.532588930442266
1106	22	0.4445512354486496
1106	23	-0.2016463130474122
1106	24	0.7027424281491292
1106	25	0.7272431403858448
1107	1	-1.1697812585368688
1107	2	-1.1683840265475496
1107	3	-0.90304548876178
1107	4	-1.0764697589126229
1107	5	-1.203815075334127
1107	6	-1.1930114036614015
1107	7	-1.045134658376199
1107	8	-1.1912076851989255
1107	9	-1.1712421956784098
1107	10	-1.0799324370623253
1107	11	-0.8724439401444144
1107	12	-0.9762788781741847
1107	13	-0.7058851600680782
1107	14	-1.2422821302664957
1107	15	-0.9932275921575382
1107	16	-1.1759407578304248
1107	17	-1.1235392855341573
1107	18	-1.2294580698883593
1107	19	-1.4327191868198739
1107	20	-1.3771260472570148
1107	21	-1.5614392553475085
1107	22	-1.4567007256014506
1107	23	-1.3471628969759166
1107	24	-0.9384598455579081
1107	25	-0.824465250908645
1108	1	0.19779771994929973
1108	2	-0.19101874619310877
1108	3	0.13656550749182372
1108	4	0.13495627864230642
1108	5	-0.1752348215190338
1108	6	0.21657573465588342
1108	7	0.15582913403132892
1108	8	0.46424695155972984
1108	9	-0.14264867692652672
1108	10	-0.5785471986629886
1108	11	-0.16845005717526362
1108	12	0.12201146097235238
1108	13	0.011203110506308178
1108	14	-0.3518533506596313
1108	15	-0.027921086843036846
1108	16	-0.8644866744091289
1108	17	-0.8102527458445152
1108	18	-0.5148039152154027
1108	19	0.1439748877328597
1108	20	-0.9533416365897267
1108	21	-0.16481480276302563
1108	22	-0.22865182725445266
1108	23	0.09602622122793747
1108	24	-0.2927132475404477
1108	25	-0.9145976539277498
1109	1	0.2703057142003793
1109	2	0.2810420082888747
1109	3	0.31029140375191844
1109	4	0.5392343671808035
1109	5	0.39253506092757817
1109	6	0.3560546427991582
1109	7	0.30027717002965204
1109	8	0.3085731790950135
1109	9	0.36319429425034144
1109	10	0.4244961866991801
1109	11	0.43955040303062276
1109	12	0.4251730023698812
1109	13	0.4692568048455925
1109	14	0.8684528688768367
1109	15	0.6695154567916077
1109	16	0.5280442936120409
1109	17	0.5994921138200766
1109	18	0.6100853705706228
1109	19	0.6349241719483416
1109	20	0.56922214849412
1109	21	0.3856690525935349
1109	22	0.5903703813320362
1109	23	0.6865726426706118
1109	24	0.8568920163383211
1109	25	0.6647166393001029
1110	1	-0.02273959984358953
1110	2	-0.0865404077811931
1110	3	0.08233393504071174
1110	4	-0.9048091329802119
1110	5	-0.4982658826218511
1110	6	-0.1966539330962931
1110	7	0.12106883772850097
1110	8	0.35373033182175817
1110	9	0.08589315389874486
1110	10	-0.06359246510960759
1110	11	-0.1087789804969006
1110	12	0.0748926624939088
1110	13	0.23895422494803853
1110	14	0.020574065984533263
1110	15	0.059417716300728785
1110	16	-0.04018275278648531
1110	17	0.19453655257606783
1110	18	0.12900452754168867
1110	19	-0.24134644067066802
1110	20	0.01724297924700835
1110	21	0.12549933480509168
1110	22	-0.34160212691851655
1110	23	0.004277406384140647
1110	24	0.16340245235834772
1110	25	0.14908118848551055
1111	1	0.1746313193145852
1111	2	-0.17153073079145997
1111	3	0.0787096686075408
1111	4	0.11421330686801667
1111	5	0.02712806862199102
1111	6	0.11529934532048003
1111	7	0.16086776036642192
1111	8	0.24470196959914148
1111	9	0.10031087681662854
1111	10	0.06313784582921045
1111	11	0.18679284033569132
1111	12	0.09556713309314091
1111	13	0.12636110340334739
1111	14	0.0186896741330429
1111	15	0.06719614582306122
1111	16	0.15575149468942584
1111	17	0.23292997392325532
1111	18	0.27967653284632377
1111	19	0.15804261965537592
1111	20	0.02443200500225373
1111	21	0.12196857954650397
1111	22	0.15682376830930167
1111	23	0.22113832126347432
1111	24	0.4973903639137802
1111	25	0.431793805976632
1112	1	-0.3329923191113373
1112	2	-0.24849354931719958
1112	3	-0.3114041590344101
1112	4	-0.3695340743903728
1112	5	-0.25356770051111965
1112	6	-0.19666446825355166
1112	7	-0.4375856502963771
1112	8	-0.011736188885095089
1112	9	-0.01758028744868767
1112	10	0.24795703624224075
1112	11	0.03315640451042157
1112	12	-0.14282977059108504
1112	13	-0.0336975603301326
1112	14	-0.357243489993067
1112	15	-0.25752291234167757
1112	16	-0.4179629568428993
1112	17	-0.37169175577457736
1112	18	-0.003976931539284578
1112	19	-0.0654246383542727
1112	20	-0.9078223626715243
1112	21	-1.5779091716639684
1112	22	-2.0404153893406587
1112	23	-0.8870150945951494
1112	24	-0.5064506205075858
1112	25	-1.6387340401800827
1113	1	-0.47678955563239
1113	2	-0.3539455828785414
1113	3	-0.5270930763778097
1113	4	-0.7330023177715645
1113	5	-0.6466092518015856
1113	6	-0.3633215546484436
1113	7	-0.5732799590203044
1113	8	-0.49040753072439003
1113	9	-0.5091532821821758
1113	10	-0.34739111413763624
1113	11	-0.6138370088875504
1113	12	-0.651751316093744
1113	13	-0.9662210633796278
1113	14	-1.126629169931384
1113	15	-0.924185945112157
1113	16	-0.9650800548530469
1113	17	-1.0926729473885999
1113	18	-1.4058211008207024
1113	19	-1.686001278215997
1113	20	-1.7674284351102685
1113	21	-1.5964886221792227
1113	22	-0.9807890992249284
1113	23	-2.7201607867433566
1113	24	-1.6480496801873983
1113	25	-1.302950139397339
1114	1	-0.1677088173897836
1114	2	-0.2662832974816105
1114	3	-0.1313279907780469
1114	4	-0.3966137664881657
1114	5	-0.5896010982934854
1114	6	-0.31479691302450163
1114	7	-0.4137573376634265
1114	8	-0.22659767729435742
1114	9	-1.4815355875403142
1114	10	-0.570260376297469
1114	11	-0.4775957519755849
1114	12	-0.29578991848747904
1114	13	-0.1535424790153712
1114	14	-0.7266665971896984
1114	15	-1.4970825734393571
1114	16	-1.4078425084714183
1114	17	-1.8235790089946704
1114	18	-2.2951953814527
1114	19	-1.8928532835926433
1114	20	-1.0142813258629175
1114	21	-0.545060349708784
1114	22	-0.42798648173150866
1114	23	-0.3306154675653019
1114	24	-1.1296861769960174
1114	25	-0.7146379075254146
1115	1	-0.3343153140331552
1115	2	-0.26081857000884057
1115	3	-0.21127461137166748
1115	4	-0.04873003216460603
1115	5	-0.01740781498722299
1115	6	-0.21822187670759519
1115	7	-0.216419001622441
1115	8	0.19036769055937697
1115	9	-0.1471385341523117
1115	10	0.184310396543502
1115	11	-0.2384650368978356
1115	12	-0.3394045896583009
1115	13	-0.20806511583770515
1115	14	-0.4587574195419961
1115	15	-1.5432824066824367
1115	16	-0.7837138993444839
1115	17	-0.6513337215968562
1115	18	-1.9637896377941928
1115	19	-0.9202349420503992
1115	20	-0.6305448533735145
1115	21	-0.4632076287343736
1115	22	-0.4289599467512346
1115	23	-0.05303825653184191
1115	24	-0.16237139322129224
1115	25	-0.49249018152250557
1116	1	-1.7616536359320847
1116	2	-0.587367672449087
1116	3	-1.589387689163818
1116	4	-0.7581215254049383
1116	5	-0.9888054320465898
1116	6	-0.22458584550995653
1116	7	-0.9849692065684366
1116	8	-3.3141105567839695
1116	9	-1.6831555411221117
1116	10	-0.8175725959409021
1116	11	-0.4942818462898728
1116	12	-0.7120706341187528
1116	13	-1.8757305872134893
1116	14	-1.8840435525303931
1116	15	-1.5894948628141705
1116	16	-1.0490875778385274
1116	17	-1.7341198955198607
1116	18	-2.523073882332149
1116	19	-1.2999908625608327
1116	20	-0.8521949005847773
1116	21	-0.4971642598697912
1116	22	-0.5453043215158573
1116	23	-0.3987856953891651
1116	24	-0.413071375210059
1116	25	-0.465077631514049
1201	1	0.29774730281889106
1201	2	0.17499915664451748
1201	3	0.4106634689274742
1201	4	0.26685129685906617
1201	5	0.4111753836691747
1201	6	0.21815673976418992
1201	7	0.07351983920526806
1201	8	0.389230066577376
1201	9	0.2570316974920175
1201	10	0.3531509073651904
1201	11	0.11847953958282176
1201	12	0.353040826572645
1201	13	0.3028738200560781
1201	14	0.6948281557661744
1201	15	0.6458515414114885
1201	16	0.4636955502229654
1201	17	0.47818746229261677
1201	18	0.5129350191757354
1201	19	0.2902635210481179
1201	20	0.33014532630136273
1201	21	0.2873041436140724
1201	22	0.5811329011113671
1201	23	0.652855839865154
1201	24	0.5927542524357676
1201	25	0.9677658630951669
1202	1	0.43362758046775696
1202	2	0.5290084482007806
1202	3	0.6213602358451418
1202	4	0.7802132733357539
1202	5	0.47943089647625214
1202	6	0.3623265626657286
1202	7	0.17125782929493738
1202	8	0.437698856030309
1202	9	0.3776502696263293
1202	10	0.5737749686036295
1202	11	0.3478512367639155
1202	12	0.4057661667723522
1202	13	0.26443412285502177
1202	14	0.8032419543759346
1202	15	0.6585513323265246
1202	16	0.6630797725416642
1202	17	0.6790747011235396
1202	18	0.7116805093776861
1202	19	0.6814160832122198
1202	20	0.5651238938447847
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2210	24	0.8350610626930433
2210	25	0.7920957657924106
2211	1	-0.18886148205053743
2211	2	-0.18173565180351403
2211	3	0.1336100202177164
2211	4	0.1485507761164726
2211	5	-0.08106284154715837
2211	6	-0.15173117474884634
2211	7	-0.16252464653722853
2211	8	-0.036893208099826325
2211	9	-0.006575364474414869
2211	10	0.15651540782011608
2211	11	0.03643644764949272
2211	12	0.004907950098981928
2211	13	-0.13809642598995708
2211	14	-0.2175060874148116
2211	15	-0.10368869514890378
2211	16	-0.2814503157777679
2211	17	0.055455789915207276
2211	18	0.03513433388623355
2211	19	-0.24068484993742345
2211	20	-0.01777460710553669
2211	21	-0.17008594872578442
2211	22	-0.0801037173527348
2211	23	-7.03213118953272E-4
2211	24	-3.599431233993755E-4
2211	25	-0.009692177441444016
2212	1	-0.09098797327397179
2212	2	0.13612562903523084
2212	3	0.09838393545999224
2212	4	0.16969208805343072
2212	5	-0.049944815528824904
2212	6	0.117141930203843
2212	7	-0.012508795506718684
2212	8	0.1886471422079481
2212	9	-0.028452617113423173
2212	10	0.2587017178134374
2212	11	0.21210549322874783
2212	12	0.2343761480255182
2212	13	0.11524455609460205
2212	14	0.17490787356901194
2212	15	0.05137967185529391
2212	16	0.06985003483192997
2212	17	0.1664271561608146
2212	18	-0.0321523470966838
2212	19	0.1814545108131611
2212	20	0.08946704940263572
2212	21	0.1083675945456406
2212	22	-0.4427583120758527
2212	23	0.34963286646831904
2212	24	0.21693692780770135
2212	25	0.2501637668836949
2213	1	0.19621929557555973
2213	2	-0.7689247510216539
2213	3	-0.1140721400818876
2213	4	0.027623421227836786
2213	5	0.15881896687882602
2213	6	0.23410700718878985
2213	7	0.15840124829490065
2213	8	0.3893794136506372
2213	9	-0.16952314555354775
2213	10	-1.3827418614362088
2213	11	-0.40622713343109496
2213	12	0.05372128917520769
2213	13	-0.11952450725670616
2213	14	0.13615062409992618
2213	15	0.09995998605506173
2213	16	0.2871066373763185
2213	17	0.05471663630996204
2213	18	0.03696122958709225
2213	19	0.02622442945168757
2213	20	0.010528588228126523
2213	21	0.26495248726168796
2213	22	0.18159681045769105
2213	23	-0.7378930423653429
2213	24	-1.4045948370137893
2213	25	-1.549830955812169
2214	1	-0.02327811197903884
2214	2	-0.1975959956727138
2214	3	-0.059120615576116364
2214	4	-0.08145152401195332
2214	5	0.05520155821515971
2214	6	-0.12634734081812127
2214	7	-0.03466338261665669
2214	8	0.09745250120227666
2214	9	-0.5014125345497433
2214	10	-0.10837888211107582
2214	11	-0.2688155122516065
2214	12	-0.1683473066528478
2214	13	-0.14352472907021507
2214	14	-0.0951823826746292
2214	15	-0.24338699497064198
2214	16	-0.23727251585060571
2214	17	-0.3788132484394353
2214	18	-0.26803696817813005
2214	19	-0.8636516322494359
2214	20	-0.7216694912365682
2214	21	-0.43284878631754964
2214	22	-0.7567344125665514
2214	23	-2.2994074741068573
2214	24	-1.5624059073563998
2214	25	-1.9745030877172738
2215	1	-0.3721908850297453
2215	2	-0.4070451671908515
2215	3	-1.561309997075842
2215	4	-1.1828547185185911
2215	5	-0.40602648577149836
2215	6	-0.3530478433806934
2215	7	-0.3040468726898027
2215	8	-0.09487968768163313
2215	9	-0.40889623858517155
2215	10	-1.5880823204351984
2215	11	-0.8010591307021215
2215	12	-0.6415920695527859
2215	13	-0.6308213405047596
2215	14	-0.9023526070862289
2215	15	-1.1290316185462999
2215	16	-0.6522399239214636
2215	17	-0.49324326286944853
2215	18	-0.5082469497674253
2215	19	-1.049296255825091
2215	20	-1.3634020881415765
2215	21	-0.8647839010112861
2215	22	-0.4901772149748709
2215	23	-0.6675270939506177
2215	24	-2.0277191381868747
2215	25	-0.802835674670547
2216	1	-0.376320520608143
2216	2	-0.23198359742963248
2216	3	-1.2939758785049733
2216	4	-0.5564386140106166
2216	5	-0.20662255244531025
2216	6	-0.27904277502933184
2216	7	-0.08528683414178317
2216	8	-0.09980883784046313
2216	9	0.1416088119198733
2216	10	0.04368410709094661
2216	11	0.015805629978892455
2216	12	-0.015557876027422424
2216	13	-0.9684163720927188
2216	14	-0.402666702099971
2216	15	-0.778879924931708
2216	16	-0.41813206083833165
2216	17	-1.9652174214160496
2216	18	-0.5531304133788026
2216	19	-0.4198748331174471
2216	20	-0.31433545002900587
2216	21	-0.10621588873302557
2216	22	0.11445568647781812
2216	23	-0.1660636967274982
2216	24	-0.37802787930833404
2216	25	0.027938498153098124
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	787.0
3	72.0
4	115.0
5	93.0
6	62.0
7	61.0
8	92.0
9	188.0
10	360.0
11	683.0
12	1144.0
13	1593.0
14	2191.0
15	2696.0
16	3044.0
17	3279.0
18	3354.0
19	3385.0
20	3722.0
21	3972.0
22	4466.0
23	4691.0
24	5133.0
25	5607.0
26	6036.0
27	6695.0
28	7672.0
29	8979.0
30	10757.0
31	13891.0
32	18024.0
33	25491.0
34	35506.0
35	55321.0
36	96655.0
37	298383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79057975827853	21.399245292567745	13.437018620966695	23.373156328187026
2	14.68926018240347	20.855249233140945	40.98723428452287	23.468256299932712
3	17.873223104460443	26.37167330715055	30.246482544637637	25.50862104375136
4	11.527175793736697	18.11803855823001	39.140032329374066	31.214753318659234
5	12.459320070240548	39.06395461866369	35.66430582139871	12.812419489697053
6	30.624173595171033	38.99193223519896	17.763142305134334	12.620751864495675
7	25.70123280486803	33.04608958845422	23.79041685359889	17.462260753078855
8	23.751131672125553	38.05878533068526	21.32283583999697	16.86724715719222
9	23.664580472782834	17.003875864880367	22.458053598486945	36.87349006384986
10	13.67489898566916	31.219077447217348	34.99212269942528	20.113900867688216
11	32.03467990754974	25.714190925908664	23.37670954787524	18.87441961866636
12	20.17319204536298	28.095914456211418	32.49096674403062	19.239926754394972
13	26.661526039436406	24.056397315707724	26.705927153730563	22.576149491125307
14	19.50614514194891	23.963130728237633	28.696920240339118	27.83380388947434
15	23.14096589203621	31.070475046999164	24.038294443830075	21.75026461713455
16	20.93212104934712	31.662730166754876	26.836080983940658	20.569067799957345
17	19.88185835325269	30.825680246583957	29.11469598037187	20.17776541979149
18	20.22235458240975	30.251061295230453	29.737279071273854	19.789305051085943
19	20.416269061356555	29.693375210915136	29.899708668533044	19.99064705919527
20	21.628384964525114	29.22922100593539	29.014839261086	20.1275547684535
21	21.52668620311056	29.650160741226372	28.51873207952544	20.30442097613763
22	20.402034184280414	29.264069537632253	29.250641014501227	21.08325526358611
23	20.27376310517635	29.78627266350268	29.01102130321855	20.92894292810243
24	20.56679202351231	29.459280093543594	29.139304111493853	20.834623771450243
25	21.066735344910672	28.835698361360272	28.109678734340015	21.987887559389037
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1221.0
1	1221.0
2	972.0
3	723.0
4	723.0
5	723.0
6	1046.0
7	1369.0
8	1369.0
9	1369.0
10	2095.0
11	2821.0
12	2821.0
13	2821.0
14	4760.5
15	6700.0
16	6700.0
17	6700.0
18	10513.0
19	14326.0
20	14326.0
21	14326.0
22	20153.0
23	25980.0
24	25980.0
25	25980.0
26	33452.5
27	40925.0
28	40925.0
29	40925.0
30	48608.5
31	56292.0
32	56292.0
33	56292.0
34	62249.0
35	68206.0
36	68206.0
37	68206.0
38	71629.5
39	75053.0
40	75053.0
41	75053.0
42	74178.5
43	73304.0
44	73304.0
45	73304.0
46	75998.5
47	78693.0
48	78693.0
49	78693.0
50	72171.5
51	65650.0
52	65650.0
53	65650.0
54	56991.5
55	48333.0
56	48333.0
57	48333.0
58	41135.0
59	33937.0
60	33937.0
61	33937.0
62	27734.5
63	21532.0
64	21532.0
65	21532.0
66	16439.0
67	11346.0
68	11346.0
69	11346.0
70	8128.0
71	4910.0
72	4910.0
73	4910.0
74	3439.5
75	1969.0
76	1969.0
77	1969.0
78	1307.0
79	645.0
80	645.0
81	645.0
82	424.5
83	204.0
84	204.0
85	204.0
86	125.5
87	47.0
88	47.0
89	47.0
90	27.0
91	7.0
92	7.0
93	7.0
94	4.5
95	2.0
96	2.0
97	2.0
98	3.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29990539262062443
2	0.17313150425733206
3	0.19142226426994638
4	0.21113213497319458
5	0.23919899085461999
6	0.18716493219804478
7	0.184799747713655
8	0.20293282876064334
9	0.206401766004415
10	0.21586250394197412
11	0.19094922737306844
12	0.1988331756543677
13	0.21018606117943867
14	0.1988331756543677
15	0.19079154840744245
16	0.1947335225480921
17	0.19410280668558816
18	0.19662567013560392
19	0.19615263323872595
20	0.19189530116682435
21	0.18842636392305268
22	0.19221065909807633
23	0.19615263323872595
24	0.21128981393882057
25	0.20450961841690318
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	634200.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.35384881644312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.13547672177187	82.23096201817461
2	4.887082101218203	8.538108620467204
3	0.49633863654880706	1.30071270656532
4	0.15514322848006823	0.542094325021711
5	0.07962648703024422	0.3477840054912211
6	0.05398281135585195	0.2829363805119396
7	0.03324515341494118	0.20328644737017612
8	0.021233701715902335	0.14838764556035053
9	0.016232199979798446	0.12761506286942262
>10	0.10463829253051767	1.685469828819107
>50	0.007053536632318537	0.4240386080510187
>100	0.007234275870825983	1.4580478117267637
>500	0.0019894258638001386	1.236552196401357
>1k	7.234275868364141E-4	1.4740043429698046
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3452	0.544307789340902	No Hit
CCCATGTACTCTGCGTTGATACCAC	3390	0.5345316934720908	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1363	0.21491643014821823	No Hit
CATGTACTCTGCGTTGATACCACTG	1125	0.1773888363292337	No Hit
GCGTTGATACCACTGCTTCCCATGT	970	0.15294859665720592	No Hit
GCTTCCCATGTACTCTGCGTTGATA	856	0.13497319457584359	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	843	0.13292336802270577	No Hit
GTATCAACGCAGAGTACATGGGAAG	739	0.1165247555976033	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	710	0.1119520655944497	No Hit
ACTCTGCGTTGATACCACTGCTTCC	682	0.10753705455692211	No Hit
TATCAACGCAGAGTACATGGGAAGC	677	0.10674865972879219	No Hit
GGTATCAACGCAGAGTACATGGGAA	664	0.10469883317565437	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAGT	40	0.005234924	14.266194	1
GACAGGC	65	5.4491204E-5	13.152151	7
GTCTTAC	70	1.0774427E-4	12.228167	1
GTCTACT	55	0.0030390227	12.10465	1
CTAGTAC	55	0.0030498437	12.098908	3
ACTGTTC	95	1.0371568E-6	11.999401	8
GCCCAGA	80	2.8270115E-5	11.888495	1
TTAGGGT	65	7.9517125E-4	11.702818	4
TAATGCT	90	7.3668343E-6	11.62155	4
CTAGGAC	90	7.3863193E-6	11.618793	3
AGCACTG	85	5.2626732E-5	11.186518	5
TAGACCC	60	0.0058300053	11.093297	5
GTCTTGG	70	0.0014752381	10.869482	1
CTAGACA	80	3.732888E-4	10.697108	4
AAAGGTG	90	9.407599E-5	10.565044	5
GGTTTAA	110	5.94461E-6	10.375414	1
CATACTG	110	5.9614704E-6	10.372953	5
CTAGACT	75	0.0026242256	10.142443	4
GAGGTGT	75	0.0026466951	10.132027	6
AACCCGA	75	0.002651904	10.129626	11
>>END_MODULE