FastQCFastQC Report
Wed 25 May 2016
SRR1295010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634200
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC37470.5908230842005676No Hit
GTACATGGGAAGCAGTGGTATCAAC34340.5414695679596342No Hit
GAGTACATGGGAAGCAGTGGTATCA15510.24456007568590352No Hit
GTATCAACGCAGAGTACTTTTTTTT14450.22784610532954902No Hit
GGTATCAACGCAGAGTACTTTTTTT14070.2218543046357616No Hit
CATGTACTCTGCGTTGATACCACTG13060.20592872910753707No Hit
TATCAACGCAGAGTACTTTTTTTTT11940.18826868495742669No Hit
GCGTTGATACCACTGCTTCCCATGT10520.16587827183853673No Hit
ACGCAGAGTACATGGGAAGCAGTGG10180.16051718700725323No Hit
GCTTCCCATGTACTCTGCGTTGATA9360.14758751182592242No Hit
GGTATCAACGCAGAGTACATGGGAA7830.12346263008514666No Hit
ACTCTGCGTTGATACCACTGCTTCC7820.12330495111952065No Hit
GTATCAACGCAGAGTACATGGGAAG7430.11715547146010723No Hit
TATCAACGCAGAGTACATGGGAAGC7350.11589403973509935No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6650.10485651214128036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAC508.705915E-515.1989913
TACTAGG456.757925E-414.7767962
CTACACC400.005276868614.2490544
TCTACAC500.001498590813.2991173
CCTATAC957.3659976E-812.9991373
CTCCTAT1102.6848284E-912.9700461
GAGTAAG751.457285E-512.6818231
GGACTGC751.4763265E-512.6658266
TAGCACC550.003066528612.0901064
CTAGGAC550.003066528612.0901063
CTAGACT658.0164033E-411.6915314
TCCTATA1251.8244464E-811.3992432
GTCTAGA700.001474506610.8701341
TTAGACT700.001490119510.8564224
ACCATTC803.7663552E-410.686798
GACTGCT1002.3939889E-510.44930657
GTTCTAG1105.9404156E-610.3760371
CCTACAC750.002645335210.1326613
GTGCTAG1151.00862835E-59.9249051
CTAATAC1151.0238795E-59.9123853
Produced by FastQC (version 0.11.5)