FastQCFastQC Report
Wed 25 May 2016
SRR1295009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6562079
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC799291.2180438546990977No Hit
CCCATGTACTCTGCGTTGATACCAC776231.182902552681856No Hit
GAGTACATGGGAAGCAGTGGTATCA353340.5384574004671385No Hit
CATGTACTCTGCGTTGATACCACTG280190.4269835824896348No Hit
GCGTTGATACCACTGCTTCCCATGT242600.36969990760550125No Hit
ACGCAGAGTACATGGGAAGCAGTGG194290.29607994661447995No Hit
GTATCAACGCAGAGTACATGGGAAG189950.28946618899284815No Hit
TATCAACGCAGAGTACATGGGAAGC189120.2882013459453932No Hit
ACTCTGCGTTGATACCACTGCTTCC174450.26584562605844886No Hit
GGTATCAACGCAGAGTACATGGGAA163940.24982936048164006No Hit
GCTTCCCATGTACTCTGCGTTGATA163070.24850356114274147No Hit
GTGGTATCAACGCAGAGTACATGGG93650.14271391734235447No Hit
CAGTGGTATCAACGCAGAGTACATG84960.12947116302622994No Hit
ATACCACTGCTTCCCATGTACTCTG83410.12710910673279002No Hit
GTACTCTGCGTTGATACCACTGCTT83410.12710910673279002No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN77390.11793518487052655No Hit
GGGAAGCAGTGGTATCAACGCAGAG75220.11462830605971064No Hit
ACATGGGAAGCAGTGGTATCAACGC74210.11308915970075946No Hit
GCAGTGGTATCAACGCAGAGTACAT72760.11087949413592857No Hit
GTATCAACGCAGAGTACTTTTTTTT70970.10815170009382698No Hit
CATGGGAAGCAGTGGTATCAACGCA70280.10710020406642469No Hit
CTGCTTCCCATGTACTCTGCGTTGA69540.10597251267471788No Hit
TACCACTGCTTCCCATGTACTCTGC68380.10420478022285316No Hit
GATACCACTGCTTCCCATGTACTCT66870.10190368022085683No Hit
GTTGATACCACTGCTTCCCATGTAC66680.10161413783649968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC700.001494124310.8557378
CGTAGAC909.473329E-510.5612073
GTCTAAG5450.09.5986711
TTACGCA900.00110783739.5062484
GTATTAG9050.09.4588611
GGCGACG850.00744751658.9400887
TCAGGAC7650.08.5732153
AACTGCG1000.00291018758.5489587
CTAGGAC6250.08.5165573
TTATACT13300.08.2911644
GTCCTAG6550.08.2771051
CAGGACA10600.08.2507074
GAAGCAG205900.08.1932099
TCTATAC8250.08.1801343
TAGACGT1050.0044891858.1482134
TTACACC5600.08.1482124
CCATGTA214100.08.1421422
CGTGCGC1050.0045211118.14180310
GTATTAA12650.08.1204141
GGAAGCA208000.08.0830888
Produced by FastQC (version 0.11.5)