##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295009_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6562079
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.972466805108564	33.0	33.0	33.0	27.0	33.0
2	31.016552680941512	33.0	33.0	33.0	27.0	33.0
3	30.982762017951934	33.0	33.0	33.0	27.0	33.0
4	34.448348305468436	37.0	37.0	37.0	27.0	37.0
5	34.809537952834766	37.0	37.0	37.0	33.0	37.0
6	34.77938942825894	37.0	37.0	37.0	33.0	37.0
7	34.67225691735805	37.0	37.0	37.0	27.0	37.0
8	34.550831527630194	37.0	37.0	37.0	27.0	37.0
9	34.46779519112769	37.0	37.0	37.0	27.0	37.0
10	34.375138275537374	37.0	37.0	37.0	27.0	37.0
11	34.586346339323256	37.0	37.0	37.0	27.0	37.0
12	34.4864875902896	37.0	37.0	37.0	27.0	37.0
13	34.40036823695661	37.0	37.0	37.0	27.0	37.0
14	35.689474479048485	37.0	37.0	40.0	27.0	40.0
15	35.83990927265582	37.0	37.0	40.0	27.0	40.0
16	35.90725347866126	37.0	37.0	40.0	27.0	40.0
17	35.84156393118705	37.0	37.0	40.0	27.0	40.0
18	35.75941969000983	37.0	37.0	40.0	27.0	40.0
19	35.820384362943514	37.0	37.0	40.0	27.0	40.0
20	35.833744610511395	37.0	37.0	40.0	27.0	40.0
21	35.819500344326855	37.0	37.0	40.0	27.0	40.0
22	35.7301442119182	37.0	37.0	40.0	27.0	40.0
23	35.63063931415638	37.0	37.0	40.0	27.0	40.0
24	35.686504688529354	37.0	37.0	40.0	27.0	40.0
25	34.42262048353883	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4616755132246375
1101	2	0.4801955323599145
1101	3	0.41743165487510225
1101	4	0.5329426095839054
1101	5	0.39219570375792756
1101	6	0.2842896273109332
1101	7	0.34427891904956454
1101	8	0.46154534736464115
1101	9	0.45480605369936455
1101	10	0.6674825979528904
1101	11	0.5456091363503646
1101	12	0.5321423114340078
1101	13	0.5427649989736452
1101	14	1.0419055532133044
1101	15	0.8603277504709439
1101	16	0.819947115100625
1101	17	0.8124278518773451
1101	18	0.8671028057709691
1101	19	0.7599100362940874
1101	20	0.7517783428886986
1101	21	0.7927107899073249
1101	22	0.8066177939124657
1101	23	0.9315158409078208
1101	24	0.9040770104323101
1101	25	0.9728511354184661
1102	1	0.4149042911329275
1102	2	0.42661117480166766
1102	3	0.4751378402798494
1102	4	0.46765393955252677
1102	5	0.3650362809822809
1102	6	0.2227764918614099
1102	7	0.2677666760799511
1102	8	0.428850103133982
1102	9	0.34630872186546213
1102	10	0.6147354401483227
1102	11	0.3794650894125624
1102	12	0.42008107931487615
1102	13	0.47433459122178334
1102	14	0.9064231682483452
1102	15	0.805558325930761
1102	16	0.7917503014155471
1102	17	0.7201496195218624
1102	18	0.8017198893508208
1102	19	0.7170821886068026
1102	20	0.7993137243145298
1102	21	0.6721691481901075
1102	22	0.7119792542308048
1102	23	0.8695923688904301
1102	24	0.7554817006396846
1102	25	0.9400615171803679
1103	1	0.40639528933629165
1103	2	0.37489323331267954
1103	3	0.3715696366097134
1103	4	0.4470070077302637
1103	5	0.28104223075892776
1103	6	0.22801845254820563
1103	7	0.2603940364084778
1103	8	0.4705916296227244
1103	9	0.47545695894158513
1103	10	0.5093657800196709
1103	11	0.38721690433381184
1103	12	0.4190371421957977
1103	13	0.4291572150014744
1103	14	0.8570850044339977
1103	15	0.7883074312699989
1103	16	0.7853213609518832
1103	17	0.6906220742103386
1103	18	0.7219671661377802
1103	19	0.696435589248587
1103	20	0.7634909722037122
1103	21	0.6840833299096403
1103	22	0.7941276302768969
1103	23	0.8001327326858885
1103	24	0.8302901016072894
1103	25	0.7619516318272659
1104	1	0.3014484746423669
1104	2	0.263097470846823
1104	3	0.2517955491026669
1104	4	0.2297835734383753
1104	5	0.23196500919002716
1104	6	0.09756717134519732
1104	7	0.1330766603628959
1104	8	0.31838327268883404
1104	9	0.26252869733902884
1104	10	0.42152497751332163
1104	11	0.2260753155564501
1104	12	0.37027418318970007
1104	13	0.3457424024201572
1104	14	0.6034060851227565
1104	15	0.5534733982096256
1104	16	0.42243299575704896
1104	17	0.504171278097509
1104	18	0.5615231946632662
1104	19	0.4785452679005644
1104	20	0.43990260373641377
1104	21	0.3913231277526421
1104	22	0.5238173793341119
1104	23	0.5131768941262465
1104	24	0.5463837719579487
1104	25	0.6858836784193727
1105	1	0.3737546098830329
1105	2	0.309036756617612
1105	3	0.27734945818214385
1105	4	0.37267425576600033
1105	5	0.30238410173827646
1105	6	0.2693811707459375
1105	7	0.32456677483150287
1105	8	0.3436112560782689
1105	9	0.41066963150497315
1105	10	0.5099301584292277
1105	11	0.3506396413943875
1105	12	0.5160130132155345
1105	13	0.47872157289400263
1105	14	0.7912615072497999
1105	15	0.5893992253906504
1105	16	0.4743530047725244
1105	17	0.5004654002953899
1105	18	0.5460268653838796
1105	19	0.5145080045605681
1105	20	0.5820860907231804
1105	21	0.5632761758162204
1105	22	0.5143866833329582
1105	23	0.617640048286745
1105	24	0.6330355196592521
1105	25	0.5319588344242092
1106	1	0.10968694173755011
1106	2	-0.02300388233273054
1106	3	-0.031900970718780286
1106	4	-0.00777995598297565
1106	5	-0.2573742421204912
1106	6	-0.3197630799346882
1106	7	-0.5654041854916301
1106	8	-0.06304604973237815
1106	9	0.13589973871776095
1106	10	-0.24757156630296606
1106	11	-0.3533552200931638
1106	12	-0.633858772932058
1106	13	-0.18185866740116552
1106	14	-0.17934621257955996
1106	15	-0.03692593600389671
1106	16	-0.054428119935430175
1106	17	-0.15423187393619742
1106	18	-0.295012720746783
1106	19	-0.4523878924301812
1106	20	0.0019468064967185228
1106	21	0.1135169301728638
1106	22	0.284673865765086
1106	23	-0.30741566595708036
1106	24	0.22816282896086193
1106	25	0.29924042966455744
1107	1	-1.154534279879794
1107	2	-1.2883827633902847
1107	3	-0.9722321507314007
1107	4	-1.0574380271922976
1107	5	-1.1352217057297267
1107	6	-1.0069008568093096
1107	7	-1.0514422179839613
1107	8	-1.0240951518618076
1107	9	-0.9408639165084978
1107	10	-1.0127731727372549
1107	11	-0.9801483943517084
1107	12	-0.940221675209088
1107	13	-0.8657725060518473
1107	14	-1.1998550415509897
1107	15	-0.9776690990323758
1107	16	-1.0163710818922453
1107	17	-1.056578155714611
1107	18	-1.2648918638392246
1107	19	-1.2720522203909042
1107	20	-1.30268891153235
1107	21	-1.295648568923383
1107	22	-1.1817237427654845
1107	23	-0.9857805682554996
1107	24	-0.836955354121315
1107	25	-0.8121292106524507
1108	1	-0.019394215803558268
1108	2	-0.13479422044003897
1108	3	-0.04280422736730927
1108	4	-0.21966682133598425
1108	5	-0.39172471329656133
1108	6	-0.0678183244289059
1108	7	-0.021341265968111145
1108	8	0.13454692486537567
1108	9	-0.28659595553840944
1108	10	-0.5599125157276674
1108	11	-0.11903002674397811
1108	12	0.06644312028549137
1108	13	0.10639814484220977
1108	14	-0.32108540565369736
1108	15	-0.026360328255520926
1108	16	-0.8596853840363323
1108	17	-0.8270055588015879
1108	18	-0.7233977557493745
1108	19	-0.11723376972139476
1108	20	-1.0343571299026948
1108	21	-0.27022038998456566
1108	22	-0.3972852313056947
1108	23	-0.18771271316636984
1108	24	-0.49112336711911553
1108	25	-1.3050845672248315
1109	1	0.06979952377412246
1109	2	0.10029709412810206
1109	3	0.09910591132904045
1109	4	0.08195587905713353
1109	5	0.04715111630315505
1109	6	0.08564519997187858
1109	7	0.1502434620303319
1109	8	0.22036855506648578
1109	9	0.27914102368057314
1109	10	0.24183970602576466
1109	11	0.22070811776542598
1109	12	0.2755678800280563
1109	13	0.2828130685960559
1109	14	0.5265201904191414
1109	15	0.4236378635352125
1109	16	0.393279878254468
1109	17	0.389286942487594
1109	18	0.4120341718686831
1109	19	0.3455717674172192
1109	20	0.28562827048171613
1109	21	0.3836266087228992
1109	22	0.3969180515986892
1109	23	0.4076425999552029
1109	24	0.557183412326232
1109	25	0.430203105475897
1110	1	0.015266818726207276
1110	2	0.04998741900441317
1110	3	0.12394417728864582
1110	4	-0.9478076388080794
1110	5	-0.23632905985035535
1110	6	0.019751999899725092
1110	7	0.049113807444385316
1110	8	0.1973884538403894
1110	9	0.12385994767910091
1110	10	-0.025502824307565675
1110	11	0.026501457880371504
1110	12	0.15900172306946558
1110	13	0.12260766138858514
1110	14	0.15094104624714078
1110	15	0.17023041125221994
1110	16	0.08783071319310665
1110	17	0.20716841091755356
1110	18	0.31021401887646505
1110	19	0.2421463264174477
1110	20	0.2188895700308109
1110	21	0.26675614738837794
1110	22	-0.3431181678473436
1110	23	0.08381157937546391
1110	24	0.22181405263072662
1110	25	0.24029638462299374
1111	1	-0.0626811813873438
1111	2	-0.24349340727083302
1111	3	-0.24989456798083864
1111	4	-0.1254045304041682
1111	5	-0.16842033476595475
1111	6	-0.12073876619382418
1111	7	-0.06291253456182488
1111	8	0.03063020057748389
1111	9	-0.059117568175409474
1111	10	-0.0764460933265596
1111	11	-0.16302158487691543
1111	12	-0.002976641141721359
1111	13	-0.18468182549194267
1111	14	-0.20347982452906166
1111	15	-0.09283542168775938
1111	16	-0.1518916792458782
1111	17	-0.06613915572863505
1111	18	-0.195926205884831
1111	19	-0.12357114017270021
1111	20	-0.09442576465061592
1111	21	-0.19039723155778887
1111	22	-0.12350745176681244
1111	23	0.09838651157225797
1111	24	0.05040615002713622
1111	25	0.013549808661913687
1112	1	-0.1823554570007957
1112	2	-0.46666636223402236
1112	3	-0.19837441062354344
1112	4	-0.18998210397266746
1112	5	-0.04102721182272262
1112	6	-0.11437819046442144
1112	7	-0.08816180411928798
1112	8	-0.020765318007072153
1112	9	-0.04273581231761625
1112	10	0.20012120340250306
1112	11	-0.10080377138054786
1112	12	-0.05328675834975627
1112	13	-0.13376581168773782
1112	14	-0.48107923185196455
1112	15	-0.3313402842277213
1112	16	-0.25866746970704213
1112	17	-0.33237624451233216
1112	18	-0.27164582769696466
1112	19	-0.1680544271391966
1112	20	-1.0579421486992473
1112	21	-1.4936717424658923
1112	22	-1.895171068152898
1112	23	-0.6878205661500871
1112	24	-0.5066644396724627
1112	25	-1.5224584433177668
1113	1	-0.21840999232693292
1113	2	-0.218013210163587
1113	3	-0.2954254460675543
1113	4	-0.6772149469692152
1113	5	-0.36026429934374704
1113	6	-0.21554061902050137
1113	7	-0.2625686505389595
1113	8	-0.12690142752522604
1113	9	-0.2917988310148445
1113	10	-0.11558772198037559
1113	11	-0.24684784890830969
1113	12	-0.2780524651827321
1113	13	-0.28489244231463573
1113	14	-0.7137114489788274
1113	15	-0.6345756995104068
1113	16	-0.56997437906697
1113	17	-0.5713930726723859
1113	18	-0.9503616193089783
1113	19	-1.289086908139879
1113	20	-1.447839061682501
1113	21	-1.1936497333531193
1113	22	-0.6053230563647958
1113	23	-2.2195147105610715
1113	24	-1.288850003873108
1113	25	-0.7510806110324211
1114	1	-0.37276141365945037
1114	2	-0.46300917307808476
1114	3	-0.3816678629979471
1114	4	-0.5948225175099395
1114	5	-0.7470261236733009
1114	6	-0.43471198015249257
1114	7	-0.46642507259846155
1114	8	-0.22626891986094932
1114	9	-1.1909771217035612
1114	10	-0.38096128386533934
1114	11	-0.398506642484854
1114	12	-0.49029414584772724
1114	13	-0.3960526790332466
1114	14	-0.8682421725916001
1114	15	-1.3928483837546324
1114	16	-1.212276600546744
1114	17	-1.6996701806961312
1114	18	-1.859070772820317
1114	19	-1.6636550637331737
1114	20	-0.9208445403632837
1114	21	-0.6864357554472704
1114	22	-0.5745333034468061
1114	23	-0.30824409590320556
1114	24	-0.91350388179535
1114	25	-0.5361440752539437
1115	1	-0.7740627739002797
1115	2	-0.6287345381179357
1115	3	-0.5741514174624491
1115	4	-0.6968140117410471
1115	5	-0.4653940101224592
1115	6	-0.4125160974161233
1115	7	-0.4083403231309646
1115	8	-0.2798519849058394
1115	9	-0.601487615602565
1115	10	-0.26262608472379867
1115	11	-0.5664252083347705
1115	12	-0.6204211592034028
1115	13	-0.5184584870176252
1115	14	-0.4957840397905997
1115	15	-1.6754659966349692
1115	16	-0.9369034676484347
1115	17	-0.7589473185178051
1115	18	-2.369842130681988
1115	19	-1.2726321754211867
1115	20	-0.9700869387650997
1115	21	-0.9846822278816632
1115	22	-0.8454874496184317
1115	23	-0.4420278634015702
1115	24	-0.4697533598779273
1115	25	-0.5454601878779854
1116	1	-1.6730841880335028
1116	2	-1.0359531820068
1116	3	-1.712938596061079
1116	4	-0.9929866894709605
1116	5	-1.1387317054504535
1116	6	-0.5249595321469442
1116	7	-1.4187858489948653
1116	8	-3.280254584686471
1116	9	-1.770685690519521
1116	10	-0.9694523280264775
1116	11	-0.7429744570239336
1116	12	-0.8275447298996284
1116	13	-1.9608303544185546
1116	14	-1.9229131812749003
1116	15	-1.7414625384519624
1116	16	-1.2904284225721696
1116	17	-1.8748900908974306
1116	18	-2.818306078318969
1116	19	-1.6202612802241703
1116	20	-1.15833245663665
1116	21	-0.839449277684146
1116	22	-0.6076702680912618
1116	23	-0.7190045835911434
1116	24	-0.6556363625923467
1116	25	-0.692487838430921
1201	1	0.3797745844083238
1201	2	0.3192727581656527
1201	3	0.3442668319501543
1201	4	0.3301090337987489
1201	5	0.2974878859010275
1201	6	0.10059726075105146
1201	7	0.16249744782292908
1201	8	0.24820014284012615
1201	9	0.2708068087514377
1201	10	0.48569684440495564
1201	11	0.37434342704882795
1201	12	0.4222164445941772
1201	13	0.41823196374100746
1201	14	0.9017434300682297
1201	15	0.7820795351975889
1201	16	0.6619364046102589
1201	17	0.6911296736322257
1201	18	0.5739428659706078
1201	19	0.4919747585738321
1201	20	0.6361930795280628
1201	21	0.6141216933365072
1201	22	0.7214703968394431
1201	23	0.7798188485887323
1201	24	0.7334490900383202
1201	25	0.912507518548594
1202	1	0.47742499503459257
1202	2	0.5283895753895358
1202	3	0.5293571835262618
1202	4	0.5465332222901722
1202	5	0.4333724138619033
1202	6	0.17740912114156515
1202	7	0.2172065429964718
1202	8	0.32453472133165207
1202	9	0.3049873497916451
1202	10	0.5556265326325232
1202	11	0.3324216926572774
1202	12	0.3754187603814714
1202	13	0.38584138072413765
1202	14	0.7607222796248365
1202	15	0.5686847717603953
1202	16	0.5336651049893248
1202	17	0.5745236265093681
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2210	20	0.6285635825006963
2210	21	0.6182830951953164
2210	22	0.6173535853642136
2210	23	0.7177324210430243
2210	24	0.6991745681058674
2210	25	0.7190495812495357
2211	1	0.1651390588724908
2211	2	0.021785393488400473
2211	3	0.10499554689103263
2211	4	0.11637319003997249
2211	5	0.11482401668441611
2211	6	0.10841361179463149
2211	7	0.13815339266428595
2211	8	0.2291917816882858
2211	9	0.10406851592393451
2211	10	0.3531585484576212
2211	11	0.14844335529267028
2211	12	0.20025282184435866
2211	13	0.09994584387703043
2211	14	0.004160910491279424
2211	15	0.12154853965894574
2211	16	0.03122087096286208
2211	17	0.1170730330681593
2211	18	0.17995166389903972
2211	19	0.09882006166558455
2211	20	0.12104356154222984
2211	21	0.11587347944077209
2211	22	0.09300192274965724
2211	23	0.24131504239274193
2211	24	0.1810694375876878
2211	25	0.22264268530365428
2212	1	0.08931300560095679
2212	2	0.14350701690587186
2212	3	0.11455726583889358
2212	4	0.061997323717157826
2212	5	0.13560820733126633
2212	6	0.15670673458712514
2212	7	0.11320288709939064
2212	8	0.1867632048132819
2212	9	0.03421481384911118
2212	10	0.2527385606031629
2212	11	0.13492892361405495
2212	12	0.1579258889743329
2212	13	0.04122107106125128
2212	14	0.14042123488066238
2212	15	0.09774035476353049
2212	16	-0.0456983149047403
2212	17	0.054970350156601455
2212	18	0.13541613547275944
2212	19	0.13027854080439738
2212	20	0.11022024510190676
2212	21	0.17718047292956385
2212	22	-0.5010013532781556
2212	23	0.06837920542618292
2212	24	0.12312125764852766
2212	25	0.23792301987786857
2213	1	-0.0478458588593611
2213	2	-0.7653795502898433
2213	3	-0.15880758490316182
2213	4	0.01029527017730203
2213	5	0.029680003428374846
2213	6	0.028192115941209295
2213	7	-0.015436514765433174
2213	8	0.2230754149702605
2213	9	-0.240332559234254
2213	10	-1.4257907829190728
2213	11	-0.409800855341949
2213	12	-0.12507390873795288
2213	13	-0.08084981650186052
2213	14	0.09113016789839179
2213	15	0.01236427633589443
2213	16	-0.016960416790183785
2213	17	-0.08523665499788535
2213	18	0.05400863293307623
2213	19	0.1553683801571708
2213	20	0.0779042607334901
2213	21	0.019275281569427705
2213	22	0.10771779507360435
2213	23	-0.8839124235533617
2213	24	-1.4177458171498003
2213	25	-1.4142718800979637
2214	1	-0.05223409122611855
2214	2	-0.28107871787064553
2214	3	5.421772653093626E-4
2214	4	0.013590271548785893
2214	5	-0.0480117422225419
2214	6	-0.037071005459850426
2214	7	-0.04190527638039043
2214	8	-0.008334432033038297
2214	9	-0.4058233237269988
2214	10	-0.05415293938048649
2214	11	-0.09701006234537601
2214	12	-0.1270931845040124
2214	13	-0.12500565696133492
2214	14	0.004192848758847845
2214	15	-0.1529234590840005
2214	16	-0.20214542747502406
2214	17	-0.12308260240348545
2214	18	4.4135938004075115E-4
2214	19	-0.7742441771443538
2214	20	-0.38224535815363225
2214	21	-0.2500596115254581
2214	22	-0.46448096572544273
2214	23	-1.8962981055302066
2214	24	-1.2878797002385696
2214	25	-1.5226893165550592
2215	1	-0.4588511654775118
2215	2	-0.2634265628757362
2215	3	-1.2150439876570616
2215	4	-0.9492399132627298
2215	5	-0.3406232671004332
2215	6	-0.29672622508341817
2215	7	-0.19969972744426912
2215	8	-0.10138149539176311
2215	9	-0.2777954885351477
2215	10	-1.330940349113689
2215	11	-0.49390568373237187
2215	12	-0.45210077269900495
2215	13	-0.4822804187029064
2215	14	-0.7238226758177575
2215	15	-0.9900401290709127
2215	16	-0.6211897265687227
2215	17	-0.48596106520560056
2215	18	-0.35051145123924243
2215	19	-0.9558470601033164
2215	20	-1.3666532766380683
2215	21	-0.6535038105417428
2215	22	-0.4086899751716686
2215	23	-0.7045331893850175
2215	24	-1.848312424772942
2215	25	-0.778324232731407
2216	1	-0.4791701805521953
2216	2	-0.48573114756667835
2216	3	-1.4079327456216397
2216	4	-0.7526556978910222
2216	5	-0.5933490767315277
2216	6	-0.464003675472334
2216	7	-0.23469548712296273
2216	8	-0.40487365615746995
2216	9	-0.16861157872848764
2216	10	-0.24390459392521535
2216	11	-0.3833868774007456
2216	12	-0.23646757466367774
2216	13	-1.071717195888617
2216	14	-0.6643217236381815
2216	15	-1.1551579185118683
2216	16	-0.8016529098987277
2216	17	-1.783046233308454
2216	18	-0.7903982711448805
2216	19	-0.6253772907215307
2216	20	-0.5416915034063905
2216	21	-0.24076975282159196
2216	22	-0.387856504313838
2216	23	-0.27209870547623893
2216	24	-0.34345700199867935
2216	25	-0.33486934387661194
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8676.0
3	931.0
4	1125.0
5	1005.0
6	655.0
7	542.0
8	935.0
9	2062.0
10	3990.0
11	6963.0
12	11550.0
13	17147.0
14	22701.0
15	29015.0
16	33736.0
17	35708.0
18	37544.0
19	38964.0
20	40340.0
21	43741.0
22	47434.0
23	50355.0
24	53897.0
25	57317.0
26	60470.0
27	68161.0
28	76896.0
29	90438.0
30	108786.0
31	137399.0
32	181940.0
33	253377.0
34	360205.0
35	562948.0
36	995494.0
37	3119632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.123665073644226	21.158948663281286	13.207044152296064	23.510342110778424
2	14.055386144883833	21.68005718335344	40.1947861410366	24.069770530726128
3	17.955746690988185	27.678382466520507	29.973061264627187	24.39280957786412
4	11.976046623830307	18.711662204185288	38.03924916682614	31.273042005158263
5	12.06338391264661	39.432019646110305	35.95557473656873	12.549021704674354
6	30.291361757194718	38.68737188076179	17.98397483614098	13.037291525902514
7	24.85179400030232	33.7393290611406	23.992454189970577	17.416422748586495
8	24.52110351677936	37.37810150665881	21.657804237288914	16.44299073927292
9	24.524896179376622	17.162535630113908	22.026725792598224	36.28584239791125
10	14.474994142485901	31.179921755219752	34.26470098574249	20.080383116551854
11	30.936252586161217	26.626653222980273	22.91511771853129	19.521976472327214
12	20.60628384057546	28.914374181473445	31.873912703532127	18.60542927441897
13	26.659511836321265	24.06223816574057	26.268266733948746	23.009983263989415
14	19.91848551467835	24.66133112125253	28.237183970640245	27.182999393428876
15	24.44546005228056	29.960465098168438	23.783715028652917	21.810359820898086
16	20.422180341251526	31.36070296506889	27.74161293859348	20.47550375508611
17	20.84129289190423	30.882033615678157	28.79874418398682	19.477929308430795
18	21.16239568401463	29.719769113290646	29.584089041555057	19.53374616113967
19	20.841424778459704	30.112667122032356	29.81955027522748	19.22635782428046
20	22.00126832842224	29.096837470568932	28.662802230095785	20.23909197091304
21	21.148016693938157	29.881428574046215	28.803532894668532	20.1670218373471
22	20.6487248508259	29.522972719571015	27.87251379047277	21.95578863913031
23	19.700692758826317	30.118997534532955	28.794440826118528	21.3858688805222
24	20.676110486861926	30.637925986430382	28.031632607709206	20.654330918998486
25	21.231527108642577	28.460478099794162	26.987701894884996	23.320292896678264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16303.0
1	16303.0
2	14928.0
3	13553.0
4	13553.0
5	13553.0
6	17762.0
7	21971.0
8	21971.0
9	21971.0
10	28003.5
11	34036.0
12	34036.0
13	34036.0
14	52695.0
15	71354.0
16	71354.0
17	71354.0
18	109202.5
19	147051.0
20	147051.0
21	147051.0
22	205614.5
23	264178.0
24	264178.0
25	264178.0
26	335917.0
27	407656.0
28	407656.0
29	407656.0
30	479414.5
31	551173.0
32	551173.0
33	551173.0
34	606046.5
35	660920.0
36	660920.0
37	660920.0
38	692474.5
39	724029.0
40	724029.0
41	724029.0
42	726120.5
43	728212.0
44	728212.0
45	728212.0
46	842565.5
47	956919.0
48	956919.0
49	956919.0
50	851586.0
51	746253.0
52	746253.0
53	746253.0
54	614786.0
55	483319.0
56	483319.0
57	483319.0
58	403218.0
59	323117.0
60	323117.0
61	323117.0
62	266226.5
63	209336.0
64	209336.0
65	209336.0
66	161746.0
67	114156.0
68	114156.0
69	114156.0
70	84143.0
71	54130.0
72	54130.0
73	54130.0
74	38244.5
75	22359.0
76	22359.0
77	22359.0
78	15243.5
79	8128.0
80	8128.0
81	8128.0
82	5451.0
83	2774.0
84	2774.0
85	2774.0
86	1807.0
87	840.0
88	840.0
89	840.0
90	530.5
91	221.0
92	221.0
93	221.0
94	135.5
95	50.0
96	50.0
97	50.0
98	45.5
99	41.0
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.31127330225680006
2	0.18166498757482194
3	0.20031761275656693
4	0.22136277237747365
5	0.24769589028111366
6	0.19816890348317964
7	0.19428293990364945
8	0.21659294257201112
9	0.2171110710492818
10	0.22756507503186107
11	0.20237488759278882
12	0.20918675316161234
13	0.22128657701316914
14	0.21022301011615374
15	0.2008814584524203
16	0.20360925249452194
17	0.20342638362019108
18	0.20703804388822505
19	0.20566652733074378
20	0.20271014719572866
21	0.19986044057073984
22	0.20351781805735653
23	0.20761712865693935
24	0.22337432999511284
25	0.21701963661211637
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	6562079.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.96774143653784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.66978039007219	50.9629591229789
2	13.51967872488376	17.29646625956852
3	3.7849838901263215	7.263506124751849
4	1.3957275854866424	3.5712616521701124
5	0.6075204672316716	1.9430856082640118
6	0.30999204160280464	1.1897694458779613
7	0.17580307283938434	0.7872007854996997
8	0.1170110741227158	0.5987947307754758
9	0.075426274703719	0.4342363593991895
>10	0.30612932207964016	3.444281314705559
>50	0.021544369173796166	0.9518004063672395
>100	0.013207324539352085	1.617569798926382
>500	0.0012493728540913717	0.5375027717224741
>1k	0.0011051870752606069	1.458203834056092
>5k	5.766193431365832E-4	2.5236127598354328
>10k+	2.6428386560426733E-4	5.419749025101008
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	79929	1.2180438546990977	No Hit
CCCATGTACTCTGCGTTGATACCAC	77623	1.182902552681856	No Hit
GAGTACATGGGAAGCAGTGGTATCA	35334	0.5384574004671385	No Hit
CATGTACTCTGCGTTGATACCACTG	28019	0.4269835824896348	No Hit
GCGTTGATACCACTGCTTCCCATGT	24260	0.36969990760550125	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	19429	0.29607994661447995	No Hit
GTATCAACGCAGAGTACATGGGAAG	18995	0.28946618899284815	No Hit
TATCAACGCAGAGTACATGGGAAGC	18912	0.2882013459453932	No Hit
ACTCTGCGTTGATACCACTGCTTCC	17445	0.26584562605844886	No Hit
GGTATCAACGCAGAGTACATGGGAA	16394	0.24982936048164006	No Hit
GCTTCCCATGTACTCTGCGTTGATA	16307	0.24850356114274147	No Hit
GTGGTATCAACGCAGAGTACATGGG	9365	0.14271391734235447	No Hit
CAGTGGTATCAACGCAGAGTACATG	8496	0.12947116302622994	No Hit
ATACCACTGCTTCCCATGTACTCTG	8341	0.12710910673279002	No Hit
GTACTCTGCGTTGATACCACTGCTT	8341	0.12710910673279002	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	7739	0.11793518487052655	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	7522	0.11462830605971064	No Hit
ACATGGGAAGCAGTGGTATCAACGC	7421	0.11308915970075946	No Hit
GCAGTGGTATCAACGCAGAGTACAT	7276	0.11087949413592857	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7097	0.10815170009382698	No Hit
CATGGGAAGCAGTGGTATCAACGCA	7028	0.10710020406642469	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	6954	0.10597251267471788	No Hit
TACCACTGCTTCCCATGTACTCTGC	6838	0.10420478022285316	No Hit
GATACCACTGCTTCCCATGTACTCT	6687	0.10190368022085683	No Hit
GTTGATACCACTGCTTCCCATGTAC	6668	0.10161413783649968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.5239072860902773E-5	0.0	0.0	0.0	0.0
3	1.5239072860902773E-5	0.0	0.0	0.0	0.0
4	1.5239072860902773E-5	0.0	0.0	1.5239072860902773E-5	0.0
5	1.5239072860902773E-5	0.0	0.0	3.0478145721805545E-5	0.0
6	1.5239072860902773E-5	0.0	0.0	4.571721858270831E-5	0.0
7	1.5239072860902773E-5	0.0	0.0	6.095629144361109E-5	0.0
8	3.0478145721805545E-5	0.0	0.0	6.095629144361109E-5	0.0
9	3.0478145721805545E-5	0.0	0.0	9.143443716541662E-5	0.0
10	3.0478145721805545E-5	0.0	0.0	1.523907286090277E-4	0.0
11	4.571721858270831E-5	0.0	0.0	1.523907286090277E-4	0.0
12	4.571721858270831E-5	0.0	0.0	1.9810794719173603E-4	0.0
13	4.571721858270831E-5	0.0	0.0	1.9810794719173603E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCC	70	0.0014941243	10.855737	8
CGTAGAC	90	9.473329E-5	10.561207	3
GTCTAAG	545	0.0	9.598671	1
TTACGCA	90	0.0011078373	9.506248	4
GTATTAG	905	0.0	9.458861	1
GGCGACG	85	0.0074475165	8.940088	7
TCAGGAC	765	0.0	8.573215	3
AACTGCG	100	0.0029101875	8.548958	7
CTAGGAC	625	0.0	8.516557	3
TTATACT	1330	0.0	8.291164	4
GTCCTAG	655	0.0	8.277105	1
CAGGACA	1060	0.0	8.250707	4
GAAGCAG	20590	0.0	8.193209	9
TCTATAC	825	0.0	8.180134	3
TAGACGT	105	0.004489185	8.148213	4
TTACACC	560	0.0	8.148212	4
CCATGTA	21410	0.0	8.142142	2
CGTGCGC	105	0.004521111	8.141803	10
GTATTAA	1265	0.0	8.120414	1
GGAAGCA	20800	0.0	8.083088	8
>>END_MODULE