FastQCFastQC Report
Wed 25 May 2016
SRR1295009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6562079
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC863411.3157567898832063No Hit
GTACATGGGAAGCAGTGGTATCAAC788811.2020733063408717No Hit
GAGTACATGGGAAGCAGTGGTATCA384680.5862166548132078No Hit
CATGTACTCTGCGTTGATACCACTG317300.48353578187644497No Hit
GCGTTGATACCACTGCTTCCCATGT260340.3967340228607428No Hit
ACGCAGAGTACATGGGAAGCAGTGG235750.35926114269578285No Hit
TATCAACGCAGAGTACATGGGAAGC210680.3210567870334996No Hit
GTATCAACGCAGAGTACATGGGAAG200000.30478145721805544No Hit
GTATCAACGCAGAGTACTTTTTTTT194390.29623233734308896No Hit
ACTCTGCGTTGATACCACTGCTTCC193610.29504368965993855No Hit
GGTATCAACGCAGAGTACATGGGAA180020.2743337896419717No Hit
GCTTCCCATGTACTCTGCGTTGATA177880.2710726280497385No Hit
GGTATCAACGCAGAGTACTTTTTTT161880.24669011147229408No Hit
TATCAACGCAGAGTACTTTTTTTTT144890.22079892668162024No Hit
CAGTGGTATCAACGCAGAGTACATG104140.15869970477344147No Hit
GTGGTATCAACGCAGAGTACATGGG101420.15455467695527592No Hit
ACATGGGAAGCAGTGGTATCAACGC96130.14649320741185834No Hit
ATACCACTGCTTCCCATGTACTCTG88220.13443910077888427No Hit
CATGGGAAGCAGTGGTATCAACGCA86840.13233610872407967No Hit
GCAGTGGTATCAACGCAGAGTACAT86270.1314674815710082No Hit
GGGAAGCAGTGGTATCAACGCAGAG85360.13008072594066605No Hit
GTACTCTGCGTTGATACCACTGCTT81560.124289878253523No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80410.12253738487451918No Hit
CTGCTTCCCATGTACTCTGCGTTGA74910.11415589480102267No Hit
TACCACTGCTTCCCATGTACTCTGC74720.1138663524166655No Hit
CCATGTACTCTGCGTTGATACCACT71470.1089136537368721No Hit
CTTCCCATGTACTCTGCGTTGATAC70870.10799930936521793No Hit
GATACCACTGCTTCCCATGTACTCT70680.10770976698086078No Hit
GCAGAGTACATGGGAAGCAGTGGTA68790.10482958221015018No Hit
AAGCAGTGGTATCAACGCAGAGTAC66890.10193415836657865No Hit
GTACTTTTTTTTTTTTTTTTTTTTT66420.1012179219421162No Hit
GTTGATACCACTGCTTCCCATGTAC65970.10053216366337558No Hit
GTACATGGTAAGCAGTGGTATCAAC65810.10028833849760113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA551.9629896E-413.8170837
AACGCCG701.09274406E-412.2133166
CGCGCGC750.00265126210.13252910
GTATCGA750.00265126210.13252918
ACCGTCT1502.4530891E-810.1325298
CACCGTC1606.195478E-910.0929497
CCTACAC5750.010.0775383
TAGACAG9250.010.0641425
CTAGGAC6100.09.8107713
GGGCCGT1451.710674E-69.1716856
TTACACT12050.09.1445714
CCTAGAC6650.09.14223
TACCGTG1252.7598284E-59.1192767
GTACCGT1054.4999298E-49.04696
CGACCAT950.00182640758.9992866
TACGACC850.00744446838.9405354
CTAGACA7900.08.8980964
TACACCG1201.7668463E-48.7077085
TACACTG11950.08.6646495
TAGGACT6800.08.6611434
Produced by FastQC (version 0.11.5)