FastQCFastQC Report
Wed 25 May 2016
SRR1295008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119390
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC4950.41460758857525754No Hit
GTACATGGGAAGCAGTGGTATCAAC4160.34843789262082253No Hit
GAGTACATGGGAAGCAGTGGTATCA1880.15746712454979478No Hit
GCGTTGATACCACTGCTTCCCATGT1650.13820252952508585No Hit
CATGTACTCTGCGTTGATACCACTG1620.1356897562609934No Hit
ACGCAGAGTACATGGGAAGCAGTGG1260.10553647709188375No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN1220.10218611273976044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGGAC350.002145196516.296163
TCTTGGA653.2874232E-614.6247582
GAGATGG400.00524824914.2471679
GTCTTGG551.884955E-413.8619891
TACAGAA550.00302535412.0986632
GTGGTAT1151.0082971E-59.90691415
GAAGCAG1252.6927648E-59.1181879
TGGTATC1252.7061556E-59.1143616
TGTACTC1554.0120976E-68.5897575
AGTGGTA1356.6817505E-58.43922214
AAGCAGT1502.5198253E-58.23169610
AGCAGTG1451.5359845E-47.857207311
ATGTACT1605.556525E-57.72694734
ATACCAC1855.2309897E-67.697939419
CCATGTA1752.0504329E-57.6048742
GTACTCT1658.273062E-57.48336036
TTGATAC1951.1012826E-57.303173516
TACTCTG1701.1970683E-47.26326137
GGTATCA1604.778412E-47.12059417
TGATACC2001.5724809E-57.120593517
Produced by FastQC (version 0.11.5)