FastQCFastQC Report
Wed 25 May 2016
SRR1295008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119390
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC6630.5553228913644358No Hit
GTACATGGGAAGCAGTGGTATCAAC4980.41712036183935003No Hit
GTATCAACGCAGAGTACTTTTTTTT2430.20353463439149008No Hit
GAGTACATGGGAAGCAGTGGTATCA2160.18091967501465783No Hit
CATGTACTCTGCGTTGATACCACTG2090.1750565373984421No Hit
GGTATCAACGCAGAGTACTTTTTTT1930.1616550799899489No Hit
GCGTTGATACCACTGCTTCCCATGT1780.14909121366948655No Hit
ACGCAGAGTACATGGGAAGCAGTGG1540.1289890275567468No Hit
TATCAACGCAGAGTACTTTTTTTTT1520.12731384538068516No Hit
GGTATCAACGCAGAGTACATGGGAA1450.1214507077644694No Hit
ACTCTGCGTTGATACCACTGCTTCC1300.10888684144400704No Hit
GCTTCCCATGTACTCTGCGTTGATA1230.10302370382779127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACT250.006003278318.9983254
AGTAGAA250.006003278318.9983252
TGTAAGA400.00524448314.2487432
GAGTGCA600.00582580411.08235556
GGAGTGC600.00582580411.08235555
CCATGTA1502.5164878E-58.2326082
CTGCGTT1656.7523867E-46.908481611
GAAGCAG1400.00749614656.78511579
CCCATGT1709.3062926E-46.71654561
GTACTCT1709.485735E-46.7052916
TGTACTC1709.485735E-46.7052915
TGCGTTG1600.00371973986.53067412
ACTCTGC1800.00181106156.3327758
TACTCTG1800.00181106156.3327757
CATGTAC1850.0024643946.16161873
GATACCA1700.00699653856.146516318
ATGTACT1700.00699653856.14651634
TCTGCGT1750.0094444055.97090210
TGATACC1750.0094444055.97090217
ATACCAC1750.0094444055.97090219
Produced by FastQC (version 0.11.5)