##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295008_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 119390 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21472485132758 33.0 33.0 33.0 33.0 33.0 2 32.18028310578775 33.0 33.0 33.0 33.0 33.0 3 32.31098081916409 33.0 33.0 33.0 33.0 33.0 4 36.13816902588157 37.0 37.0 37.0 33.0 37.0 5 36.19677527431108 37.0 37.0 37.0 33.0 37.0 6 35.82004355473658 37.0 37.0 37.0 33.0 37.0 7 35.670793198760364 37.0 37.0 37.0 33.0 37.0 8 35.67209984085769 37.0 37.0 37.0 33.0 37.0 9 35.634860541083846 37.0 37.0 37.0 33.0 37.0 10 35.638135522238045 37.0 37.0 37.0 33.0 37.0 11 35.633051344333694 37.0 37.0 37.0 33.0 37.0 12 35.62635061562945 37.0 37.0 37.0 33.0 37.0 13 35.57352374570735 37.0 37.0 37.0 33.0 37.0 14 37.29090376078398 40.0 37.0 40.0 33.0 40.0 15 37.35678029985761 40.0 37.0 40.0 33.0 40.0 16 37.404263338638074 40.0 37.0 40.0 33.0 40.0 17 37.33187034089957 40.0 37.0 40.0 33.0 40.0 18 37.343403970181754 40.0 37.0 40.0 33.0 40.0 19 37.28558505737499 40.0 37.0 40.0 33.0 40.0 20 37.23943378842449 40.0 37.0 40.0 33.0 40.0 21 37.251838512438226 40.0 37.0 40.0 33.0 40.0 22 37.229533461763964 40.0 37.0 40.0 33.0 40.0 23 37.18478096993048 40.0 37.0 40.0 33.0 40.0 24 37.219314850489994 40.0 37.0 40.0 33.0 40.0 25 36.219683390568726 37.0 37.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32690845002640856 1101 2 0.4204462355793339 1101 3 0.26845341926187416 1101 4 0.28063988684974817 1101 5 0.2533738086853745 1101 6 0.5968491589144307 1101 7 0.5037627483569267 1101 8 0.7376766552243623 1101 9 0.7128777553333094 1101 10 0.5144650849682151 1101 11 0.478700876010528 1101 12 0.5973991544689881 1101 13 0.5590066350031222 1101 14 1.1637739620178635 1101 15 1.176268750600208 1101 16 0.7280392216889808 1101 17 0.8753559650599456 1101 18 0.8687989806322634 1101 19 0.7269463485133443 1101 20 0.9340264943990348 1101 21 1.1481512425513003 1101 22 0.7075555334539843 1101 23 1.1801752172354654 1101 24 1.071640634613317 1101 25 0.6170033917784608 1102 1 -0.0862973787531871 1102 2 -0.3300091378268597 1102 3 -0.321983739208072 1102 4 -0.0791961787240254 1102 5 -0.08906699277181929 1102 6 -0.4798357226884846 1102 7 -0.4496980895301377 1102 8 -0.5008479349395714 1102 9 -0.6536978366521211 1102 10 -0.14008864909371255 1102 11 -0.3748510365577786 1102 12 -0.29941323168765877 1102 13 0.0772215712508455 1102 14 -0.46263769554133205 1102 15 -0.5822922694362234 1102 16 -0.11458372913069326 1102 17 -0.628104872827123 1102 18 -0.33411540916737437 1102 19 -0.007115582269896947 1102 20 -0.10249445277764835 1102 21 -0.1903733476126348 1102 22 -0.1225901860359997 1102 23 0.2241642882737196 1102 24 -0.27498960218085244 1102 25 0.6578048489733632 1103 1 -0.13497911133323015 1103 2 -0.026807631235520546 1103 3 0.15795976361678754 1103 4 -0.08964815612515054 1103 5 0.21790099216226366 1103 6 -0.1523404307864169 1103 7 -0.5993214416958637 1103 8 -0.5779665790073665 1103 9 -0.2360895503997682 1103 10 -0.5795425097340186 1103 11 0.0816461385834657 1103 12 -0.1362305574880338 1103 13 -0.09321157375857325 1103 14 -0.12158308160536535 1103 15 -0.3891660924117346 1103 16 -0.0874650850628953 1103 17 -0.5045643831849347 1103 18 -0.0015353715025909764 1103 19 -0.3988293299158485 1103 20 -0.4170895563558048 1103 21 -0.04319820636969496 1103 22 -0.24641316155388893 1103 23 -0.14278486426865555 1103 24 -0.2491892255330157 1103 25 -0.237863700932472 1104 1 0.1540567116009015 1104 2 0.3195635117457911 1104 3 0.24413347690914122 1104 4 0.32684777731993364 1104 5 0.10751420380937304 1104 6 0.4616783188255553 1104 7 0.7517060618740103 1104 8 0.4429249588333235 1104 9 0.42353049057620495 1104 10 0.5202898612847946 1104 11 0.18815262644588415 1104 12 0.5489994667250429 1104 13 0.9166599106891837 1104 14 0.6275942946906525 1104 15 0.8932266560827031 1104 16 0.898969384422422 1104 17 0.6342882895660438 1104 18 0.8696696946177269 1104 19 0.622310547156232 1104 20 0.6216203213371259 1104 21 0.7325204252811375 1104 22 0.7452364317906159 1104 23 1.0115269256363533 1104 24 0.9024218752306297 1104 25 0.8960221871907024 1105 1 -0.09319603565184309 1105 2 -0.11999692781464688 1105 3 -0.20641597364030417 1105 4 -0.41491046443830726 1105 5 -0.16721107091589715 1105 6 -0.5822777251304885 1105 7 -0.5230192128512599 1105 8 -0.5955365796282166 1105 9 -0.8237222566765396 1105 10 -0.4687211277261909 1105 11 -0.21294627465301375 1105 12 -0.44550602778045345 1105 13 -0.41850492447565557 1105 14 -0.19108702399066146 1105 15 -0.35402609117004147 1105 16 -0.7493822639292276 1105 17 -0.05252489724714593 1105 18 -0.7072533823053462 1105 19 -0.2842828594371767 1105 20 -0.37288517316837044 1105 21 -0.8279191351584245 1105 22 -0.13937895282476376 1105 23 -0.40605549194606283 1105 24 -0.009666036857282734 1105 25 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 7.0 11 15.0 12 40.0 13 85.0 14 108.0 15 168.0 16 172.0 17 233.0 18 281.0 19 264.0 20 289.0 21 353.0 22 371.0 23 442.0 24 459.0 25 561.0 26 586.0 27 722.0 28 833.0 29 972.0 30 1263.0 31 1703.0 32 2510.0 33 3508.0 34 5402.0 35 9238.0 36 18173.0 37 70629.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.47746871120357 20.837667348924604 12.636311780693218 26.048552159178605 2 15.08694341139813 20.5665561027909 39.698294635976815 24.648205849834152 3 18.185777703325236 24.709774687997317 30.19599631459921 26.90845129407823 4 12.404724013736494 17.878381774017924 37.63129240304883 32.08560180919675 5 12.977124812584284 37.72940102356203 35.73755936576009 13.555914798093593 6 31.979227741016835 37.98810620654996 16.853170282268195 13.179495770165007 7 27.114498701733815 32.28076053270793 22.416450288968925 18.188290476589327 8 24.363216041678882 38.05962023301979 19.949074035296384 17.62808969000494 9 24.196331351034424 16.93776698215931 21.560432197001425 37.30546946980484 10 13.84621827623754 31.126560013401456 34.42331853589078 20.603903174470222 11 33.1820085434291 24.326995560767234 22.915654577435298 19.575341318368373 12 21.060390317447023 26.63288382611609 31.807521567970515 20.499204288466373 13 27.67065918418628 22.77745204791021 26.516458664879806 23.035430103023703 14 20.566211575508834 23.012815143646872 27.981405477845716 28.439567802998578 15 23.57316358153949 30.503392243906525 23.666973783398944 22.25647039115504 16 21.534466873272468 30.389479855934333 26.450288968925374 21.62576430186783 17 20.32163497780384 30.024290141552896 28.540916324650308 21.113158555992964 18 21.144987017338135 29.007454560683477 29.29726107714214 20.550297344836252 19 21.093056369880227 29.049334115085017 29.436301197755256 20.421308317279504 20 21.93399782226317 28.16064997068431 29.041795795292742 20.863556411759777 21 21.759778875952758 28.609598793868834 28.277075131920597 21.35354719825781 22 21.244660356813803 28.38428679118854 28.38428679118854 21.986766060809114 23 21.051176815478684 28.63807689086188 28.53589077812212 21.774855515537315 24 21.429947482598898 28.797460402549646 28.65674392113176 21.11584819371969 25 21.56815116970575 28.030220539580696 27.933059159554062 22.468569131159487 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 35.0 1 35.0 2 33.0 3 31.0 4 31.0 5 31.0 6 69.0 7 107.0 8 107.0 9 107.0 10 164.0 11 221.0 12 221.0 13 221.0 14 424.0 15 627.0 16 627.0 17 627.0 18 1174.5 19 1722.0 20 1722.0 21 1722.0 22 2783.0 23 3844.0 24 3844.0 25 3844.0 26 5237.0 27 6630.0 28 6630.0 29 6630.0 30 8252.5 31 9875.0 32 9875.0 33 9875.0 34 11127.5 35 12380.0 36 12380.0 37 12380.0 38 13227.5 39 14075.0 40 14075.0 41 14075.0 42 14230.0 43 14385.0 44 14385.0 45 14385.0 46 14982.0 47 15579.0 48 15579.0 49 15579.0 50 14624.5 51 13670.0 52 13670.0 53 13670.0 54 12023.5 55 10377.0 56 10377.0 57 10377.0 58 8784.5 59 7192.0 60 7192.0 61 7192.0 62 5859.0 63 4526.0 64 4526.0 65 4526.0 66 3516.0 67 2506.0 68 2506.0 69 2506.0 70 1781.0 71 1056.0 72 1056.0 73 1056.0 74 715.5 75 375.0 76 375.0 77 375.0 78 256.5 79 138.0 80 138.0 81 138.0 82 84.5 83 31.0 84 31.0 85 31.0 86 18.0 87 5.0 88 5.0 89 5.0 90 4.0 91 3.0 92 3.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14909121366948655 2 0.0016751821760616465 3 0.0 4 0.0 5 0.00251277326409247 6 0.0 7 0.0 8 8.375910880308232E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 8.375910880308232E-4 25 8.375910880308232E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 119390.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.29638080038355 #Duplication Level Percentage of deduplicated Percentage of total 1 94.24189220545574 84.15459893727075 2 5.0351300404374255 8.99237778940702 3 0.44005045980609 1.1788474029068547 4 0.0939775583485238 0.33567423347920183 5 0.03599048350695044 0.16069099602132847 6 0.023993611135552356 0.12855255820419637 7 0.013996271279862712 0.08748714589944646 8 0.01299653749035705 0.09284350086603076 9 0.007997869219188242 0.06427626978494695 >10 0.06798188835939055 1.2980235591970126 >50 0.010997070175777453 0.6177663706768154 >100 0.013996271132807669 2.296983918715963 >500 9.997336523434048E-4 0.5918773175704172 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 663 0.5553228913644358 No Hit GTACATGGGAAGCAGTGGTATCAAC 498 0.41712036183935003 No Hit GTATCAACGCAGAGTACTTTTTTTT 243 0.20353463439149008 No Hit GAGTACATGGGAAGCAGTGGTATCA 216 0.18091967501465783 No Hit CATGTACTCTGCGTTGATACCACTG 209 0.1750565373984421 No Hit GGTATCAACGCAGAGTACTTTTTTT 193 0.1616550799899489 No Hit GCGTTGATACCACTGCTTCCCATGT 178 0.14909121366948655 No Hit ACGCAGAGTACATGGGAAGCAGTGG 154 0.1289890275567468 No Hit TATCAACGCAGAGTACTTTTTTTTT 152 0.12731384538068516 No Hit GGTATCAACGCAGAGTACATGGGAA 145 0.1214507077644694 No Hit ACTCTGCGTTGATACCACTGCTTCC 130 0.10888684144400704 No Hit GCTTCCCATGTACTCTGCGTTGATA 123 0.10302370382779127 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.375910880308234E-4 0.0 9 0.0 0.0 0.0 8.375910880308234E-4 0.0 10 0.0 0.0 0.0 8.375910880308234E-4 0.0 11 0.0 0.0 0.0 8.375910880308234E-4 0.0 12 0.0 0.0 0.0 8.375910880308234E-4 0.0 13 0.0 0.0 0.0 8.375910880308234E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCACT 25 0.0060032783 18.998325 4 AGTAGAA 25 0.0060032783 18.998325 2 TGTAAGA 40 0.005244483 14.248743 2 GAGTGCA 60 0.005825804 11.0823555 6 GGAGTGC 60 0.005825804 11.0823555 5 CCATGTA 150 2.5164878E-5 8.232608 2 CTGCGTT 165 6.7523867E-4 6.9084816 11 GAAGCAG 140 0.0074961465 6.7851157 9 CCCATGT 170 9.3062926E-4 6.7165456 1 GTACTCT 170 9.485735E-4 6.705291 6 TGTACTC 170 9.485735E-4 6.705291 5 TGCGTTG 160 0.0037197398 6.530674 12 ACTCTGC 180 0.0018110615 6.332775 8 TACTCTG 180 0.0018110615 6.332775 7 CATGTAC 185 0.002464394 6.1616187 3 GATACCA 170 0.0069965385 6.1465163 18 ATGTACT 170 0.0069965385 6.1465163 4 TCTGCGT 175 0.009444405 5.970902 10 TGATACC 175 0.009444405 5.970902 17 ATACCAC 175 0.009444405 5.970902 19 >>END_MODULE