FastQCFastQC Report
Wed 25 May 2016
SRR1295007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357789
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1078337.941808336936004No Hit
GTACATGGGAAGCAGTGGTATCAAC1064177.837521146510983No Hit
GAGTACATGGGAAGCAGTGGTATCA510843.7622929630450686No Hit
CATGTACTCTGCGTTGATACCACTG438403.2287785510119758No Hit
GCGTTGATACCACTGCTTCCCATGT309182.2770842892378713No Hit
ACGCAGAGTACATGGGAAGCAGTGG282782.0826505443776613No Hit
TATCAACGCAGAGTACATGGGAAGC265531.955605767906501No Hit
ACTCTGCGTTGATACCACTGCTTCC253591.867668687844724No Hit
GGTATCAACGCAGAGTACATGGGAA237661.7503455986165743No Hit
GTATCAACGCAGAGTACATGGGAAG237361.748136124243163No Hit
GCTTCCCATGTACTCTGCGTTGATA156231.1506206045269185No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA145611.072405211708152No Hit
GTGGTATCAACGCAGAGTACATGGG128000.9427090659888981No Hit
GTACTCTGCGTTGATACCACTGCTT121530.8950580686689906No Hit
ATACCACTGCTTCCCATGTACTCTG121270.8931431908787005No Hit
CAGTGGTATCAACGCAGAGTACATG109340.8052797599627041No Hit
GTTGATACCACTGCTTCCCATGTAC104860.7722849426530927No Hit
GGGAAGCAGTGGTATCAACGCAGAG104240.767718695614709No Hit
ACATGGGAAGCAGTGGTATCAACGC100360.7391428270519205No Hit
CATGGGAAGCAGTGGTATCAACGCA99610.7336191411183918No Hit
CTGCTTCCCATGTACTCTGCGTTGA98450.7250758402078674No Hit
GATACCACTGCTTCCCATGTACTCT97430.7175636273382683No Hit
TACCACTGCTTCCCATGTACTCTGC97120.7152805038190765No Hit
GCAGTGGTATCAACGCAGAGTACAT96120.7079155892410381No Hit
GCAGAGTACATGGGAAGCAGTGGTA92480.681107300176979No Hit
CCACTGCTTCCCATGTACTCTGCGT84970.6257967916959115No Hit
AAGCAGTGGTATCAACGCAGAGTAC82910.6106250676651527No Hit
CTTCCCATGTACTCTGCGTTGATAC82560.6080473475628393No Hit
CCATGTACTCTGCGTTGATACCACT74080.5455928719410748No Hit
TTCCCATGTACTCTGCGTTGATACC70670.5204785132299643No Hit
ATGGGAAGCAGTGGTATCAACGCAG70420.5186372845854548No Hit
GTACATGGTAAGCAGTGGTATCAAC70230.5172379508156274No Hit
TGATACCACTGCTTCCCATGTACTC63000.4639896184164108No Hit
ACCATGTACTCTGCGTTGATACCAC56820.41847444632413433No Hit
GTATCAACGCAGAGTACTTTTTTTT52950.38997222690712624No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA50540.372222782774054No Hit
GGTATCAACGCAGAGTACTTTTTTT46360.341437439837854No Hit
ATCAACGCAGAGTACATGGGAAGCA44320.326413014098656No Hit
TATCAACGCAGAGTACTTTTTTTTT41860.3082953242366818No Hit
GAACAAAAAAAAAAAAAAAAAAAAA41080.302550690865812No Hit
TCCATGTACTCTGCGTTGATACCAC34410.25342671063029676No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33630.24768207725942692No Hit
CCCCATGTACTCTGCGTTGATACCA33290.2451780063028939No Hit
GGAAGCAGTGGTATCAACGCAGAGT32710.24090635584763173No Hit
GTACATGGGTAAGCAGTGGTATCAA30500.22462989463016714No Hit
AAACAAAAAAAAAAAAAAAAAAAAA30280.2230096134229987No Hit
GGTAAGCAGTGGTATCAACGCAGAG29030.21380347020045087No Hit
AAAAAGTACTCTGCGTTGATACCAC28240.20798518768380064No Hit
CTGCGTTGATACCACTGCTTCCCAT24800.18264988153534903No Hit
GAGTACATGGTAAGCAGTGGTATCA24590.18110324947396098No Hit
CTCTGCGTTGATACCACTGCTTCCC24060.1771998447476007No Hit
GTACATGGAAAGCAGTGGTATCAAC23100.17012952675268397No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22050.1623963664457438No Hit
AAAGTACTCTGCGTTGATACCACTG21540.15864026001094425No Hit
TCAACGCAGAGTACATGGGAAGCAG21480.15819836513626195No Hit
AGTGGTATCAACGCAGAGTACATGG20850.15355846895209785No Hit
TGGGAAGCAGTGGTATCAACGCAGA20770.1529692757858548No Hit
GCTTACCATGTACTCTGCGTTGATA20070.14781383558122801No Hit
TCCCATGTACTCTGCGTTGATACCA19310.14221650050191892No Hit
AAAAACAAAAAAAAAAAAAAAAAAA18280.13463063848653953No Hit
ACTCTGCGTTGATACCACTGCTTAC17960.13227386582156728No Hit
GCGTTGATACCACTGCTTACCATGT17520.12903330340723043No Hit
AACGCAGAGTACATGGGAAGCAGTG16640.12255217857855676No Hit
GTACATGGAAGCAGTGGTATCAACG16510.12159473968341178No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16450.12115284480872948No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN16220.11945891445578069No Hit
TTGATACCACTGCTTCCCATGTACT16010.11791228239439264No Hit
GAGTACATGGAAAGCAGTGGTATCA15920.1172494400823692No Hit
ACTGCTTCCCATGTACTCTGCGTTG15870.1168811943534673No Hit
GAAAACAAAAAAAAAAAAAAAAAAA15580.11474536912583619No Hit
CTGCTTACCATGTACTCTGCGTTGA15150.11157845585727975No Hit
ACGCAGAGTACATGGTAAGCAGTGG14870.10951627977542902No Hit
ATGTACTCTGCGTTGATACCACTGC14600.10752775283935868No Hit
TATCAACGCAGAGTACATGGTAAGC14450.10642301565265296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAC250.006017823719.007573
GTTCTAA402.7346268E-416.6457611
GTACATA8800.016.1053161
GTACAAA5300.015.4343461
TACATAA7500.015.3327732
TACATAG2150.015.02924252
TCCATAC400.005264708314.2556773
AGTACAA1152.5465852E-1114.0490742
AGAACAA9500.014.0055792
ACATAAA13100.012.8409923
GTACAAG1352.7284841E-1112.6824871
CATAAAA13000.012.0651694
CCTGTAC802.8540646E-511.8797313
TACATGA3450.011.8452972
TACAAAA7150.011.8298872
TACAAGA1052.7046553E-711.7665922
ATGGTCC658.045169E-411.688333511
GAGAACA13900.011.6332161
TATCAAA2700.011.6256121
CTGTACT907.4071686E-611.6183124
Produced by FastQC (version 0.11.5)