##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295007_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1357789 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.731445754826414 33.0 27.0 33.0 15.0 33.0 2 29.729343071714382 33.0 27.0 33.0 15.0 33.0 3 30.01883355955896 33.0 27.0 33.0 22.0 33.0 4 32.836138015553225 37.0 33.0 37.0 15.0 37.0 5 34.115584232896275 37.0 33.0 37.0 27.0 37.0 6 33.35535639189889 37.0 33.0 37.0 22.0 37.0 7 33.711970711207705 37.0 33.0 37.0 27.0 37.0 8 33.32583486830428 37.0 33.0 37.0 15.0 37.0 9 33.0958764579769 37.0 33.0 37.0 15.0 37.0 10 33.446292465176846 37.0 33.0 37.0 22.0 37.0 11 33.57277750814007 37.0 33.0 37.0 22.0 37.0 12 33.669810994197185 37.0 33.0 37.0 27.0 37.0 13 33.203242919187005 37.0 33.0 37.0 15.0 37.0 14 34.70816599633669 37.0 33.0 40.0 22.0 40.0 15 34.613091577557334 37.0 33.0 40.0 15.0 40.0 16 34.65522478087538 37.0 33.0 40.0 22.0 40.0 17 34.75697402173681 37.0 33.0 40.0 22.0 40.0 18 34.61844734343849 37.0 33.0 40.0 22.0 40.0 19 34.53432307965376 37.0 33.0 40.0 22.0 40.0 20 34.66308535420452 37.0 33.0 40.0 22.0 40.0 21 34.613118827741275 37.0 33.0 40.0 22.0 40.0 22 34.69587837285469 37.0 33.0 40.0 22.0 40.0 23 34.746320672799676 37.0 33.0 40.0 22.0 40.0 24 34.91950001067912 37.0 33.0 40.0 27.0 40.0 25 33.832033548658885 37.0 33.0 40.0 15.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6641628422942389 1101 2 0.6066700807658414 1101 3 0.5413194090172055 1101 4 0.5849104749826068 1101 5 0.3950374035169375 1101 6 0.426977658725157 1101 7 0.320093647641265 1101 8 0.4011647531839273 1101 9 0.2797607364522108 1101 10 0.5303482904562102 1101 11 0.5230635261850551 1101 12 0.42019256224553914 1101 13 0.4662277117290117 1101 14 1.032661577477299 1101 15 0.7736122479060796 1101 16 1.0107944575558037 1101 17 0.8818352992509162 1101 18 0.7585612563564723 1101 19 0.7398740576333154 1101 20 0.6510878065992998 1101 21 0.6145759752280071 1101 22 0.6748608187382104 1101 23 0.8439925948290821 1101 24 0.704757956952939 1101 25 1.0406761763989465 1102 1 0.6516146872273296 1102 2 0.7076282164653911 1102 3 0.3022683685449259 1102 4 0.5164930034857846 1102 5 0.18535503749821203 1102 6 0.2935746149391676 1102 7 0.1876563726852325 1102 8 0.3411839427096339 1102 9 0.2932856039052254 1102 10 0.5283688747381419 1102 11 0.2315211714764942 1102 12 0.5748510015466977 1102 13 0.43868934704102003 1102 14 0.6662994973370928 1102 15 0.6952763578844454 1102 16 0.7834543901051987 1102 17 0.6679317300374379 1102 18 0.6873364891190761 1102 19 0.8353751890602297 1102 20 0.7212373694452694 1102 21 0.7824222204528581 1102 22 0.7093680399805251 1102 23 0.7035999017320833 1102 24 0.5850537831626994 1102 25 0.700836078169587 1103 1 0.6296976776611665 1103 2 0.6691485753062452 1103 3 0.5965576337364311 1103 4 0.8336457374272968 1103 5 0.4469959062146529 1103 6 0.49552820583979695 1103 7 0.5075595768254075 1103 8 0.5594312878148386 1103 9 0.6009031589584097 1103 10 0.7531449831232067 1103 11 0.48924769192020534 1103 12 0.47097838584657126 1103 13 0.5691148172194787 1103 14 0.9758212862392455 1103 15 1.0619151938554978 1103 16 0.9698155172241201 1103 17 0.8542713038586953 1103 18 0.9220796505944904 1103 19 1.0623314135044168 1103 20 0.9970850943195799 1103 21 1.0926463774427688 1103 22 0.8870270231659916 1103 23 1.0541362690153662 1103 24 1.0134153364980847 1103 25 1.0955653269913626 1104 1 0.535166111955224 1104 2 0.4559972189520387 1104 3 0.4825928559282353 1104 4 0.4618576441445441 1104 5 0.3397319483457153 1104 6 0.26519827479812363 1104 7 0.1644302506355828 1104 8 0.34176361740695427 1104 9 0.3554860849292396 1104 10 0.47376938837395244 1104 11 0.5356155826297311 1104 12 0.4469728066096792 1104 13 0.4624486719768157 1104 14 0.8571151999175015 1104 15 0.6689830962970618 1104 16 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23 0.10739253461012765 2211 24 -0.05413336227295673 2211 25 -0.19551765038982438 2212 1 -0.10584399577924586 2212 2 -0.12004556416992784 2212 3 -0.03213447933566371 2212 4 -0.2425511476982578 2212 5 0.006052666427571296 2212 6 0.04307110152426219 2212 7 -0.04193129781164373 2212 8 0.096593028584266 2212 9 -0.021191344089167785 2212 10 0.3605252605127731 2212 11 0.019459184578046518 2212 12 0.10943239231460922 2212 13 -0.18697115959792399 2212 14 -0.18272880145831039 2212 15 -0.11707910343886851 2212 16 -0.19792449941124346 2212 17 -0.08334101291194429 2212 18 -0.06535890997335514 2212 19 -0.04301454029801732 2212 20 -0.07281710195794489 2212 21 -0.14232124166938576 2212 22 -0.7266714816089248 2212 23 -0.11813238621039801 2212 24 -0.09694014601222989 2212 25 -0.19622498115889186 2213 1 0.15228182196433693 2213 2 -0.5219501974217735 2213 3 -0.13587909513254814 2213 4 0.06818720291065006 2213 5 0.09603026931408465 2213 6 0.13895201682597502 2213 7 0.2148246278319874 2213 8 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2214 19 -0.7440550727769448 2214 20 -0.22346261680895196 2214 21 -0.27797301263530727 2214 22 -0.5039054307133455 2214 23 -1.5911818299087912 2214 24 -1.2657800021017422 2214 25 -1.736127156958787 2215 1 -0.7092052030043234 2215 2 -0.6067643332212782 2215 3 -1.4535952696716556 2215 4 -1.236650514363614 2215 5 -0.7370622378602363 2215 6 -0.5499258167965522 2215 7 -0.683879087085522 2215 8 -0.36091840645153894 2215 9 -0.7501659101345197 2215 10 -1.7960592143958287 2215 11 -0.9388421165338485 2215 12 -0.8161644482922839 2215 13 -0.9599454045349063 2215 14 -1.1281720618403526 2215 15 -1.3987530890861777 2215 16 -1.1153971558051694 2215 17 -0.9908080463494002 2215 18 -0.7685949668123868 2215 19 -1.5481395473956852 2215 20 -1.8460893155284737 2215 21 -1.085025646995014 2215 22 -0.7643071984005942 2215 23 -1.0466122602447854 2215 24 -2.1340771124387885 2215 25 -1.080101585121021 2216 1 -0.5954665067921461 2216 2 -0.7274243867646462 2216 3 -1.5483697333950097 2216 4 -1.0775223474485607 2216 5 -0.8667960045756828 2216 6 -0.7964242556768681 2216 7 -0.5081990053543279 2216 8 -0.7014709349259576 2216 9 -0.4802579251590373 2216 10 -0.6592928226390811 2216 11 -0.6133903021958247 2216 12 -0.5797316910784289 2216 13 -1.2610317819954382 2216 14 -0.8764072885230263 2216 15 -1.2981879839887682 2216 16 -1.0073422165303683 2216 17 -1.8598702281406077 2216 18 -1.068579962530265 2216 19 -1.063027167008471 2216 20 -0.8656189902895832 2216 21 -0.4674003359511829 2216 22 -0.6777695797993317 2216 23 -0.5095543808050351 2216 24 -0.7477704586297236 2216 25 -0.7761355656053368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1805.0 3 210.0 4 250.0 5 203.0 6 123.0 7 99.0 8 128.0 9 225.0 10 569.0 11 1047.0 12 2063.0 13 4107.0 14 7904.0 15 14089.0 16 19302.0 17 20598.0 18 19057.0 19 17706.0 20 16983.0 21 17268.0 22 16856.0 23 15899.0 24 15173.0 25 14785.0 26 14077.0 27 14265.0 28 14882.0 29 15763.0 30 18835.0 31 23746.0 32 31305.0 33 43683.0 34 63127.0 35 101073.0 36 192383.0 37 618201.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.73527169351884 19.158471140320806 9.871127318289814 23.235129847870542 2 9.95869543082038 28.85557272275175 31.2216944170212 29.96403742940667 3 23.020076527694243 35.77466763586987 23.54455321331449 17.6607026231214 4 13.315111688154479 26.062906748528746 24.012860948376844 36.609120614939926 5 8.607457557042817 46.92014928403466 32.859763818186515 11.61262934073601 6 31.030973559084877 30.126437299040116 19.284769150273164 19.55781999160184 7 19.296429235578355 40.14172956411112 21.26637129219934 19.295469908111187 8 28.221559409905133 37.538132005937264 20.797175740478714 13.44313284367889 9 27.044234984979443 23.12722817221603 16.25541588857478 33.57312095422974 10 16.96915296700344 38.93226408172334 27.856888191408242 16.241694759864973 11 23.138858835632714 34.32998622880262 13.097692844733807 29.433462090830865 12 23.550359093656613 40.464773690918925 23.45470903118148 12.530158184242982 13 30.2103800895492 26.277434222083784 17.428509004374124 26.083676683992895 14 19.87108239715301 36.89871539970508 21.248894812741796 21.98130739040011 15 38.36767285562789 24.062855384351984 17.871114433669092 19.698357326351044 16 14.402421780675938 31.856791996670196 35.091244808256015 18.649541414397845 17 27.271050038928596 35.71859237147106 24.046360083982453 12.963997505617893 18 28.132590095891207 26.16052127975982 27.843654449203047 17.863234175145926 19 21.581506289243194 38.823789014414636 28.29746157229604 11.297243124046135 20 23.58340737941804 30.501279693463967 25.000295200337707 20.91501772678028 21 15.944282498800783 35.03051547913361 30.597025940002215 18.428176082063395 22 20.22169496278639 33.783537451614926 15.846614244122758 30.148153341475926 23 13.436824474173834 34.59806651546943 26.16794702143158 25.79716198892516 24 19.436511387064222 44.669932732430375 18.905486591882273 16.988069288623123 25 20.483022399626822 27.50331403467853 16.224819979274365 35.788843586420285 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18794.0 1 18794.0 2 19169.5 3 19545.0 4 19545.0 5 19545.0 6 22838.0 7 26131.0 8 26131.0 9 26131.0 10 24337.5 11 22544.0 12 22544.0 13 22544.0 14 21608.5 15 20673.0 16 20673.0 17 20673.0 18 21694.5 19 22716.0 20 22716.0 21 22716.0 22 24853.5 23 26991.0 24 26991.0 25 26991.0 26 30648.5 27 34306.0 28 34306.0 29 34306.0 30 36452.5 31 38599.0 32 38599.0 33 38599.0 34 39675.5 35 40752.0 36 40752.0 37 40752.0 38 41621.5 39 42491.0 40 42491.0 41 42491.0 42 65264.5 43 88038.0 44 88038.0 45 88038.0 46 293135.0 47 498232.0 48 498232.0 49 498232.0 50 410925.0 51 323618.0 52 323618.0 53 323618.0 54 206316.0 55 89014.0 56 89014.0 57 89014.0 58 54804.5 59 20595.0 60 20595.0 61 20595.0 62 16388.0 63 12181.0 64 12181.0 65 12181.0 66 9495.5 67 6810.0 68 6810.0 69 6810.0 70 5076.5 71 3343.0 72 3343.0 73 3343.0 74 2436.5 75 1530.0 76 1530.0 77 1530.0 78 1070.0 79 610.0 80 610.0 81 610.0 82 406.5 83 203.0 84 203.0 85 203.0 86 124.5 87 46.0 88 46.0 89 46.0 90 29.5 91 13.0 92 13.0 93 13.0 94 11.5 95 10.0 96 10.0 97 10.0 98 7.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30969465800650914 2 0.18346002213893323 3 0.20062027310576236 4 0.22021094588334417 5 0.24480976057399198 6 0.20106216798044468 7 0.19686416667096285 8 0.21770687492681115 9 0.21940080527975994 10 0.22625017583733556 11 0.20526016928992646 12 0.20990006547409062 13 0.22116838477848916 14 0.2073223453717772 15 0.20194595772980925 16 0.2027560983333935 17 0.20209325602137004 18 0.2073959945175576 19 0.20452367783212266 20 0.20452367783212266 21 0.20172501029246814 22 0.2043027303947815 23 0.2081324859753614 24 0.22462989463016714 25 0.21689673432322693 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1357789.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.005371649622337 #Duplication Level Percentage of deduplicated Percentage of total 1 77.58759570599045 14.745810917929909 2 11.349283263291344 4.313946927513812 3 4.348110105552232 2.479123455884963 4 2.1605048821828814 1.6424479294683676 5 1.1662316167251694 1.108233265270088 6 0.7357666124948382 0.8390110750708838 7 0.4685658273470359 0.6233687383730234 8 0.3202884586162522 0.4869760952869246 9 0.265435072326299 0.4540222978565128 >10 1.2930011272637965 4.604426323791046 >50 0.1401508140201664 1.864384836144013 >100 0.11445649804653502 4.217839587270503 >500 0.017518851741816586 2.324820855201671 >1k 0.019076083007755835 7.691789773242587 >5k 0.00661823288024182 9.917242867757588 >10k+ 0.007396848513211446 42.68655505393812 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 107833 7.941808336936004 No Hit GTACATGGGAAGCAGTGGTATCAAC 106417 7.837521146510983 No Hit GAGTACATGGGAAGCAGTGGTATCA 51084 3.7622929630450686 No Hit CATGTACTCTGCGTTGATACCACTG 43840 3.2287785510119758 No Hit GCGTTGATACCACTGCTTCCCATGT 30918 2.2770842892378713 No Hit ACGCAGAGTACATGGGAAGCAGTGG 28278 2.0826505443776613 No Hit TATCAACGCAGAGTACATGGGAAGC 26553 1.955605767906501 No Hit ACTCTGCGTTGATACCACTGCTTCC 25359 1.867668687844724 No Hit GGTATCAACGCAGAGTACATGGGAA 23766 1.7503455986165743 No Hit GTATCAACGCAGAGTACATGGGAAG 23736 1.748136124243163 No Hit GCTTCCCATGTACTCTGCGTTGATA 15623 1.1506206045269185 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 14561 1.072405211708152 No Hit GTGGTATCAACGCAGAGTACATGGG 12800 0.9427090659888981 No Hit GTACTCTGCGTTGATACCACTGCTT 12153 0.8950580686689906 No Hit ATACCACTGCTTCCCATGTACTCTG 12127 0.8931431908787005 No Hit CAGTGGTATCAACGCAGAGTACATG 10934 0.8052797599627041 No Hit GTTGATACCACTGCTTCCCATGTAC 10486 0.7722849426530927 No Hit GGGAAGCAGTGGTATCAACGCAGAG 10424 0.767718695614709 No Hit ACATGGGAAGCAGTGGTATCAACGC 10036 0.7391428270519205 No Hit CATGGGAAGCAGTGGTATCAACGCA 9961 0.7336191411183918 No Hit CTGCTTCCCATGTACTCTGCGTTGA 9845 0.7250758402078674 No Hit GATACCACTGCTTCCCATGTACTCT 9743 0.7175636273382683 No Hit TACCACTGCTTCCCATGTACTCTGC 9712 0.7152805038190765 No Hit GCAGTGGTATCAACGCAGAGTACAT 9612 0.7079155892410381 No Hit GCAGAGTACATGGGAAGCAGTGGTA 9248 0.681107300176979 No Hit CCACTGCTTCCCATGTACTCTGCGT 8497 0.6257967916959115 No Hit AAGCAGTGGTATCAACGCAGAGTAC 8291 0.6106250676651527 No Hit CTTCCCATGTACTCTGCGTTGATAC 8256 0.6080473475628393 No Hit CCATGTACTCTGCGTTGATACCACT 7408 0.5455928719410748 No Hit TTCCCATGTACTCTGCGTTGATACC 7067 0.5204785132299643 No Hit ATGGGAAGCAGTGGTATCAACGCAG 7042 0.5186372845854548 No Hit GTACATGGTAAGCAGTGGTATCAAC 7023 0.5172379508156274 No Hit TGATACCACTGCTTCCCATGTACTC 6300 0.4639896184164108 No Hit ACCATGTACTCTGCGTTGATACCAC 5682 0.41847444632413433 No Hit GTATCAACGCAGAGTACTTTTTTTT 5295 0.38997222690712624 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 5054 0.372222782774054 No Hit GGTATCAACGCAGAGTACTTTTTTT 4636 0.341437439837854 No Hit ATCAACGCAGAGTACATGGGAAGCA 4432 0.326413014098656 No Hit TATCAACGCAGAGTACTTTTTTTTT 4186 0.3082953242366818 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 4108 0.302550690865812 No Hit TCCATGTACTCTGCGTTGATACCAC 3441 0.25342671063029676 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3363 0.24768207725942692 No Hit CCCCATGTACTCTGCGTTGATACCA 3329 0.2451780063028939 No Hit GGAAGCAGTGGTATCAACGCAGAGT 3271 0.24090635584763173 No Hit GTACATGGGTAAGCAGTGGTATCAA 3050 0.22462989463016714 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 3028 0.2230096134229987 No Hit GGTAAGCAGTGGTATCAACGCAGAG 2903 0.21380347020045087 No Hit AAAAAGTACTCTGCGTTGATACCAC 2824 0.20798518768380064 No Hit CTGCGTTGATACCACTGCTTCCCAT 2480 0.18264988153534903 No Hit GAGTACATGGTAAGCAGTGGTATCA 2459 0.18110324947396098 No Hit CTCTGCGTTGATACCACTGCTTCCC 2406 0.1771998447476007 No Hit GTACATGGAAAGCAGTGGTATCAAC 2310 0.17012952675268397 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2205 0.1623963664457438 No Hit AAAGTACTCTGCGTTGATACCACTG 2154 0.15864026001094425 No Hit TCAACGCAGAGTACATGGGAAGCAG 2148 0.15819836513626195 No Hit AGTGGTATCAACGCAGAGTACATGG 2085 0.15355846895209785 No Hit TGGGAAGCAGTGGTATCAACGCAGA 2077 0.1529692757858548 No Hit GCTTACCATGTACTCTGCGTTGATA 2007 0.14781383558122801 No Hit TCCCATGTACTCTGCGTTGATACCA 1931 0.14221650050191892 No Hit AAAAACAAAAAAAAAAAAAAAAAAA 1828 0.13463063848653953 No Hit ACTCTGCGTTGATACCACTGCTTAC 1796 0.13227386582156728 No Hit GCGTTGATACCACTGCTTACCATGT 1752 0.12903330340723043 No Hit AACGCAGAGTACATGGGAAGCAGTG 1664 0.12255217857855676 No Hit GTACATGGAAGCAGTGGTATCAACG 1651 0.12159473968341178 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1645 0.12115284480872948 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 1622 0.11945891445578069 No Hit TTGATACCACTGCTTCCCATGTACT 1601 0.11791228239439264 No Hit GAGTACATGGAAAGCAGTGGTATCA 1592 0.1172494400823692 No Hit ACTGCTTCCCATGTACTCTGCGTTG 1587 0.1168811943534673 No Hit GAAAACAAAAAAAAAAAAAAAAAAA 1558 0.11474536912583619 No Hit CTGCTTACCATGTACTCTGCGTTGA 1515 0.11157845585727975 No Hit ACGCAGAGTACATGGTAAGCAGTGG 1487 0.10951627977542902 No Hit ATGTACTCTGCGTTGATACCACTGC 1460 0.10752775283935868 No Hit TATCAACGCAGAGTACATGGTAAGC 1445 0.10642301565265296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.4729829156076533E-4 0.0 9 0.0 0.0 0.0 2.20947437341148E-4 0.0 10 0.0 0.0 0.0 4.41894874682296E-4 0.0 11 0.0 0.0 0.0 6.62842312023444E-4 0.0 12 0.0 0.0 0.0 0.00110473718670574 0.0 13 0.0 0.0 0.0 0.0011783863324861226 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGCAC 25 0.0060178237 19.00757 3 GTTCTAA 40 2.7346268E-4 16.645761 1 GTACATA 880 0.0 16.105316 1 GTACAAA 530 0.0 15.434346 1 TACATAA 750 0.0 15.332773 2 TACATAG 215 0.0 15.0292425 2 TCCATAC 40 0.0052647083 14.255677 3 AGTACAA 115 2.5465852E-11 14.049074 2 AGAACAA 950 0.0 14.005579 2 ACATAAA 1310 0.0 12.840992 3 GTACAAG 135 2.7284841E-11 12.682487 1 CATAAAA 1300 0.0 12.065169 4 CCTGTAC 80 2.8540646E-5 11.879731 3 TACATGA 345 0.0 11.845297 2 TACAAAA 715 0.0 11.829887 2 TACAAGA 105 2.7046553E-7 11.766592 2 ATGGTCC 65 8.045169E-4 11.6883335 11 GAGAACA 1390 0.0 11.633216 1 TATCAAA 270 0.0 11.625612 1 CTGTACT 90 7.4071686E-6 11.618312 4 >>END_MODULE