FastQCFastQC Report
Wed 25 May 2016
SRR1295007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357789
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1173478.642506309890564No Hit
GTACATGGGAAGCAGTGGTATCAAC1049787.731540025733012No Hit
GAGTACATGGGAAGCAGTGGTATCA552314.067715970596315No Hit
CATGTACTCTGCGTTGATACCACTG486423.5824417490493734No Hit
ACGCAGAGTACATGGGAAGCAGTGG347612.5601179564718817No Hit
GCGTTGATACCACTGCTTCCCATGT324442.3894728856987353No Hit
TATCAACGCAGAGTACATGGGAAGC297122.18826341942673No Hit
ACTCTGCGTTGATACCACTGCTTCC282032.0771268584441325No Hit
GGTATCAACGCAGAGTACATGGGAA258741.9055979979216213No Hit
GTATCAACGCAGAGTACATGGGAAG250211.8427752765709546No Hit
GTATCAACGCAGAGTACTTTTTTTT192591.4184088985843897No Hit
GCTTCCCATGTACTCTGCGTTGATA171851.265660570235876No Hit
GGTATCAACGCAGAGTACTTTTTTT169691.2497523547473135No Hit
TATCAACGCAGAGTACTTTTTTTTT164821.213885220752267No Hit
GTACTTTTTTTTTTTTTTTTTTTTT153831.1329448095396266No Hit
GTGGTATCAACGCAGAGTACATGGG138041.0166528083524025No Hit
CAGTGGTATCAACGCAGAGTACATG137111.0098034377948266No Hit
ACATGGGAAGCAGTGGTATCAACGC131780.9705484430938829No Hit
CATGGGAAGCAGTGGTATCAACGCA129430.9532408938354928No Hit
ATACCACTGCTTCCCATGTACTCTG125510.9243704286895829No Hit
GGGAAGCAGTGGTATCAACGCAGAG123980.9131021093851842No Hit
GCAGTGGTATCAACGCAGAGTACAT114110.8404104024999467No Hit
GTACTCTGCGTTGATACCACTGCTT112130.8258278716354308No Hit
GCAGAGTACATGGGAAGCAGTGGTA107790.7938641423667449No Hit
ACGCAGAGTACTTTTTTTTTTTTTT106890.7872357192465103No Hit
GATACCACTGCTTCCCATGTACTCT106400.7836269111032715No Hit
CTGCTTCCCATGTACTCTGCGTTGA104470.7694126259676577No Hit
TACCACTGCTTCCCATGTACTCTGC102460.7546091476658008No Hit
GTTGATACCACTGCTTCCCATGTAC98080.7223508218139931No Hit
AAGCAGTGGTATCAACGCAGAGTAC96460.7104196601975712No Hit
CTTCCCATGTACTCTGCGTTGATAC94160.6934803566680833No Hit
CCACTGCTTCCCATGTACTCTGCGT92690.682653932238367No Hit
CCATGTACTCTGCGTTGATACCACT85010.626091388279033No Hit
GAGTACTTTTTTTTTTTTTTTTTTT83920.6180636313889714No Hit
ATGGGAAGCAGTGGTATCAACGCAG82430.6070899086676943No Hit
GTACATGGTAAGCAGTGGTATCAAC71930.5297583055982925No Hit
TTCCCATGTACTCTGCGTTGATACC67720.49875201522475143No Hit
ACCATGTACTCTGCGTTGATACCAC62940.4635477235417285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA59800.44042189176668833No Hit
TGATACCACTGCTTCCCATGTACTC58970.43430901266691657No Hit
ATCAACGCAGAGTACATGGGAAGCA51870.3820181191628449No Hit
GGAAGCAGTGGTATCAACGCAGAGT38590.2842120535664967No Hit
CCCCATGTACTCTGCGTTGATACCA38000.27986675396545413No Hit
TCCATGTACTCTGCGTTGATACCAC34950.2574037645024374No Hit
GCAGAGTACTTTTTTTTTTTTTTTT34760.25600443073261014No Hit
GGTAAGCAGTGGTATCAACGCAGAG33790.24886046359191305No Hit
GTACATGGGTAAGCAGTGGTATCAA31010.22838600106496665No Hit
GTGGTATCAACGCAGAGTACTTTTT29010.2136561719088901No Hit
GAGTACATGGTAAGCAGTGGTATCA29000.2135825227631097No Hit
AAAAAGTACTCTGCGTTGATACCAC28390.20908992487050637No Hit
CTGCGTTGATACCACTGCTTCCCAT28250.20805883682958104No Hit
CTCTGCGTTGATACCACTGCTTCCC26510.19524388546379445No Hit
TGGGAAGCAGTGGTATCAACGCAGA25790.1899411469676069No Hit
TCAACGCAGAGTACATGGGAAGCAG25440.1873634268652935No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA25120.18500665420032125No Hit
GTACATGGAAAGCAGTGGTATCAAC23580.17366468575014232No Hit
ATCAACGCAGAGTACTTTTTTTTTT23420.1724862994176562No Hit
AGTGGTATCAACGCAGAGTACATGG22380.1648267882564964No Hit
AAAGTACTCTGCGTTGATACCACTG21770.16033419036389307No Hit
GCTTACCATGTACTCTGCGTTGATA21150.15576794332550933No Hit
AACGCAGAGTACATGGGAAGCAGTG20780.15304292493163518No Hit
TCCCATGTACTCTGCGTTGATACCA20540.151275345432906No Hit
ACTCTGCGTTGATACCACTGCTTAC19990.14722464241498495No Hit
GAACAAAAAAAAAAAAAAAAAAAAA18820.13860769235868017No Hit
ACGCAGAGTACATGGTAAGCAGTGG18740.1380184991924371No Hit
GTACATGGAAGCAGTGGTATCAACG18220.13418874361185723No Hit
GAGTACATGGAAAGCAGTGGTATCA18180.1338941470287357No Hit
ACTGCTTCCCATGTACTCTGCGTTG18100.13330495386249264No Hit
GCGTTGATACCACTGCTTACCATGT17980.13242116411312801No Hit
ATGGTAAGCAGTGGTATCAACGCAG15640.11518726400051849No Hit
CAACGCAGAGTACATGGGAAGCAGT15380.11327238621022853No Hit
CTGCTTACCATGTACTCTGCGTTGA15130.11143115756571896No Hit
TATCAACGCAGAGTACATGGTAAGC14880.10958992892120942No Hit
TTGATACCACTGCTTCCCATGTACT14480.1066439630899941No Hit
CGCAGAGTACATGGGAAGCAGTGGT13920.10251961092629268No Hit
CGTTGATACCACTGCTTCCCATGTA13720.10104662801068502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACGC804.311005E-1016.623877
GTACAAA2350.016.1899831
AATACTT456.7650294E-414.7767755
TACATAA1250.014.439022
CGCAAAA2450.014.3459652
GGACGCA903.6405254E-813.7212928
TGGGACG903.6405254E-813.7212926
ACGCAAA3750.013.4430831
CTCCAAA500.001485633413.3162611
TTGCTCT500.001500143913.2990974
TTATACT500.001500143913.2990974
GTACATA1800.013.2105761
GAACAAA8200.012.7594831
CATTTAC1051.9588697E-812.6821541
CGCAAAG1207.403287E-1012.6658082
TTTACTC853.945268E-612.2932833
CAAGTAC701.0913954E-412.2134583
AAAGCAT550.003069661312.09008915
GCAAAGA1201.0017175E-811.8741953
TACAAGA802.8677263E-511.8741942
Produced by FastQC (version 0.11.5)