##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295007_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1357789 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76180982464875 33.0 33.0 33.0 27.0 33.0 2 31.622358849570883 33.0 33.0 33.0 27.0 33.0 3 31.900522835285894 33.0 33.0 33.0 27.0 33.0 4 35.507217248040746 37.0 37.0 37.0 33.0 37.0 5 35.9100456698353 37.0 37.0 37.0 33.0 37.0 6 35.292224344135946 37.0 37.0 37.0 33.0 37.0 7 35.29983303738651 37.0 37.0 37.0 33.0 37.0 8 35.241452096017866 37.0 37.0 37.0 33.0 37.0 9 35.109913248671184 37.0 37.0 37.0 33.0 37.0 10 35.26326623650655 37.0 37.0 37.0 33.0 37.0 11 35.203425569068536 37.0 37.0 37.0 33.0 37.0 12 35.254323757225905 37.0 37.0 37.0 33.0 37.0 13 35.025071642206555 37.0 37.0 37.0 33.0 37.0 14 36.6670425228073 40.0 37.0 40.0 33.0 40.0 15 36.60951443854678 40.0 37.0 40.0 33.0 40.0 16 36.48230689746345 37.0 37.0 40.0 33.0 40.0 17 36.60132244406163 40.0 37.0 40.0 33.0 40.0 18 36.639284159762674 40.0 37.0 40.0 33.0 40.0 19 36.4008325299439 40.0 37.0 40.0 33.0 40.0 20 36.5123181878775 40.0 37.0 40.0 33.0 40.0 21 36.517447850881105 40.0 37.0 40.0 33.0 40.0 22 36.622835359544084 40.0 37.0 40.0 33.0 40.0 23 36.59994962398429 40.0 37.0 40.0 33.0 40.0 24 36.61525244349453 40.0 37.0 40.0 33.0 40.0 25 35.60757820250422 37.0 33.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1872895089041826 1101 2 0.19458502303609393 1101 3 0.18497655144082614 1101 4 0.23655043537574727 1101 5 0.31362958977739197 1101 6 0.11633693177200399 1101 7 0.2614978902616443 1101 8 0.34451123352224755 1101 9 0.2793096192327198 1101 10 0.3840657693594167 1101 11 0.28128648628496933 1101 12 0.30289143946245645 1101 13 0.4170254067399739 1101 14 0.6455154858061007 1101 15 0.51510156695511 1101 16 0.5671691998447344 1101 17 0.6150219030987429 1101 18 0.5312551273715727 1101 19 0.49532689983430345 1101 20 0.5379826088951063 1101 21 0.4757847875600163 1101 22 0.5994953459994079 1101 23 0.5864008274866563 1101 24 0.6052087052641468 1101 25 0.8939717046167743 1102 1 0.1023138897793352 1102 2 0.1486806352446166 1102 3 0.15833309605674373 1102 4 0.09658946556874781 1102 5 0.20955746517624618 1102 6 0.11862714448464118 1102 7 0.09555144638389379 1102 8 0.10617152419951026 1102 9 0.08413321047557787 1102 10 0.2652198439599971 1102 11 0.3961711179481284 1102 12 0.38594920037256486 1102 13 0.38420978568905895 1102 14 0.7082458647622971 1102 15 0.6202499903033001 1102 16 0.5987509349400852 1102 17 0.74926650635242 1102 18 0.5732381654067211 1102 19 0.591697223208179 1102 20 0.64606390529066 1102 21 0.576449909543733 1102 22 0.565064456061215 1102 23 0.6164003979520771 1102 24 0.525937961958661 1102 25 0.5251735888782676 1103 1 0.1620782206343101 1103 2 0.13214117344662313 1103 3 -0.04218881704628785 1103 4 0.039429850736951266 1103 5 0.22315193826412383 1103 6 0.06153623078916581 1103 7 0.20897352003204617 1103 8 0.08108887403836462 1103 9 0.11641115964697235 1103 10 0.26752146673472055 1103 11 0.2169893942824146 1103 12 0.39377196976116835 1103 13 0.115629293955962 1103 14 0.34128399874244764 1103 15 0.4500029167696411 1103 16 0.2968301858767788 1103 17 0.4650515219071707 1103 18 0.46814724955591913 1103 19 0.3071670217845295 1103 20 0.4015465927390167 1103 21 0.41753717642124144 1103 22 0.4265327906298211 1103 23 0.5058896359864278 1103 24 0.5678677472420119 1103 25 0.6096571274124685 1104 1 0.09580783621445832 1104 2 0.1224012336710949 1104 3 0.1746031217385351 1104 4 0.11662615384072694 1104 5 -0.0474063764934769 1104 6 0.1883658424586372 1104 7 0.24534232323359362 1104 8 0.26355442746476854 1104 9 0.27552369598370774 1104 10 0.2508494554719576 1104 11 0.28759176936877395 1104 12 0.1334197478431136 1104 13 0.2185638682784372 1104 14 0.5406350865632845 1104 15 0.4165436595381422 1104 16 0.48758220965371635 1104 17 0.44709211476626365 1104 18 0.403738348848087 1104 19 0.44549038288369047 1104 20 0.45027653420916636 1104 21 0.4033497505612189 1104 22 0.3727632861129848 1104 23 0.3819093458139662 1104 24 0.4476557883823702 1104 25 0.3934382336135016 1105 1 0.10350404269424374 1105 2 0.10092437297477375 1105 3 0.09759635084428453 1105 4 0.13453146528886606 1105 5 0.1223658043582816 1105 6 0.28797830298341154 1105 7 0.10499585098646236 1105 8 0.25339002859281834 1105 9 0.20941325633044272 1105 10 0.05548433402473307 1105 11 0.029098520769089475 1105 12 0.15606650470172667 1105 13 0.20637391935255067 1105 14 0.35990575338590247 1105 15 0.09895414987565232 1105 16 0.24567492281992287 1105 17 0.30605581705485463 1105 18 0.38915688368374646 1105 19 0.10620749986031086 1105 20 0.37789970340354984 1105 21 0.31983564344853477 1105 22 0.25501444600226364 1105 23 0.23787644004765696 1105 24 0.2788328323217044 1105 25 0.06300559816313012 1106 1 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-0.0950350914716509 2214 3 0.008348764475002213 2214 4 0.049569846233964654 2214 5 0.02334660141546152 2214 6 -0.0023392322831341517 2214 7 0.058378400306366984 2214 8 0.10308822699857245 2214 9 0.07294034661136806 2214 10 -0.02713604526583424 2214 11 -0.05764550100610677 2214 12 -0.10865591349860182 2214 13 -0.05327035254661183 2214 14 -0.20609915778563703 2214 15 -0.2883125240336355 2214 16 -0.208211721186089 2214 17 -0.02334607090127605 2214 18 -0.18569515342945664 2214 19 -0.1931486539204812 2214 20 -0.12440835276929363 2214 21 -0.033308575821877184 2214 22 7.019529507346078E-4 2214 23 0.11149810567795271 2214 24 -0.010919947808048391 2214 25 0.0409030012617535 2215 1 -0.20958089270368419 2215 2 -0.17638116040541618 2215 3 -0.12422589238459736 2215 4 -0.1453455998957338 2215 5 -0.20540171448881495 2215 6 -0.2541516400588506 2215 7 -0.3300806851305964 2215 8 -0.3337690333881014 2215 9 -0.2984513485795688 2215 10 -0.23597901018003142 2215 11 -0.2924603353892081 2215 12 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-0.5524223061406417 2216 24 -0.4640150817311266 2216 25 -0.7525054401990232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 35.0 9 65.0 10 110.0 11 225.0 12 455.0 13 1008.0 14 2069.0 15 3806.0 16 5534.0 17 6583.0 18 6724.0 19 6693.0 20 6809.0 21 7091.0 22 7487.0 23 7482.0 24 8265.0 25 8644.0 26 8898.0 27 9485.0 28 10484.0 29 12477.0 30 15968.0 31 21944.0 32 29686.0 33 43005.0 34 64706.0 35 108015.0 36 214939.0 37 739095.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.61403410448444 18.30881637204034 10.170360188427129 24.906789335048096 2 10.018405165524968 26.32365840778747 31.499788992400045 32.15814743428752 3 23.794860615309155 31.49318487629521 26.395411952814463 18.31654255558117 4 13.833298104491934 23.14689543073335 25.958451570899456 37.06135489387526 5 9.085773710359984 42.055793934519684 36.40017322368392 12.458259131436417 6 33.85246161222399 27.349683934690884 18.912658741527586 19.88519571155754 7 19.22544666365687 35.4664089928553 24.458365769644622 20.84977857384321 8 30.185472009804247 32.410944638265875 23.237213125258236 14.166370226671646 9 28.309921497375512 15.78382208133959 19.92194663530195 35.98430978598295 10 18.269701698864846 32.84170073553402 31.590696345308437 17.297901220292697 11 25.101912005473604 27.554354910814567 15.530027124980391 31.813705958731436 12 24.86034280731395 35.36447857509525 27.105684314720474 12.66949430287033 13 33.38731879334764 19.471516961966394 21.65170466639122 25.489459578294753 14 22.060938776201606 29.55709613202051 25.869704350234095 22.512260741543788 15 40.39257940666775 17.248040748599376 22.361869185860247 19.99751065887262 16 15.250381318452277 25.487317985342344 39.57617862569221 19.686122070513164 17 27.354692076603946 30.245273750192407 29.03897439145552 13.361059781748121 18 28.360518460526634 19.465984773775602 33.628641858197405 18.544854907500355 19 22.173916565828712 32.610737014366734 34.08386722826595 11.131479191538597 20 23.64041835660769 23.283219999572836 31.703747784081326 21.37261385973815 21 15.209432393398384 30.040750072360282 35.892248353757466 18.85756918048386 22 20.016880384212865 28.560549540466155 21.804492450594314 29.61807762472667 23 13.154326629542586 27.526810130292706 32.772249591063115 26.54661364910159 24 19.414162214552853 38.583955303575976 25.194766170121568 16.807116311749603 25 20.718909336497322 21.886035945466826 22.166052060018604 35.22900265801725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7400.0 1 7400.0 2 7519.0 3 7638.0 4 7638.0 5 7638.0 6 15417.0 7 23196.0 8 23196.0 9 23196.0 10 19439.5 11 15683.0 12 15683.0 13 15683.0 14 11696.0 15 7709.0 16 7709.0 17 7709.0 18 10329.0 19 12949.0 20 12949.0 21 12949.0 22 19394.5 23 25840.0 24 25840.0 25 25840.0 26 32880.5 27 39921.0 28 39921.0 29 39921.0 30 41785.0 31 43649.0 32 43649.0 33 43649.0 34 44489.0 35 45329.0 36 45329.0 37 45329.0 38 41696.5 39 38064.0 40 38064.0 41 38064.0 42 59638.5 43 81213.0 44 81213.0 45 81213.0 46 300692.5 47 520172.0 48 520172.0 49 520172.0 50 434226.5 51 348281.0 52 348281.0 53 348281.0 54 222358.0 55 96435.0 56 96435.0 57 96435.0 58 58277.5 59 20120.0 60 20120.0 61 20120.0 62 16191.0 63 12262.0 64 12262.0 65 12262.0 66 9456.5 67 6651.0 68 6651.0 69 6651.0 70 4913.0 71 3175.0 72 3175.0 73 3175.0 74 2269.5 75 1364.0 76 1364.0 77 1364.0 78 960.5 79 557.0 80 557.0 81 557.0 82 347.5 83 138.0 84 138.0 85 138.0 86 85.0 87 32.0 88 32.0 89 32.0 90 19.5 91 7.0 92 7.0 93 7.0 94 5.0 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12675017988803858 2 0.001325684624046888 3 0.0 4 0.0 5 5.155440204626787E-4 6 0.0 7 0.0 8 7.364914578038267E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0011783863324861226 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 5.155440204626787E-4 25 7.364914578038267E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1357789.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.469972681634047 #Duplication Level Percentage of deduplicated Percentage of total 1 74.86336144907953 10.832707950234761 2 12.753619976753921 3.69089065311143 3 5.016523836901406 2.177668886301881 4 2.469805375048569 1.429520652236229 5 1.3427290508950482 0.9714626342643877 6 0.8025733081310233 0.6967928306198743 7 0.519440070441051 0.5261398540319651 8 0.36168249566196553 0.4186828665323092 9 0.27495303574926927 0.3580706624421848 >10 1.3050135264116156 3.48610390280384 >50 0.12295569043464698 1.2629121947895672 >100 0.1020379173731447 2.838987044065317 >500 0.021427962648360388 2.2594663373898247 >1k 0.022958531408957556 6.883229509427971 >5k 0.006632464629254406 7.426576312119898 >10k+ 0.014285308432240258 54.74078770962858 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 117347 8.642506309890564 No Hit GTACATGGGAAGCAGTGGTATCAAC 104978 7.731540025733012 No Hit GAGTACATGGGAAGCAGTGGTATCA 55231 4.067715970596315 No Hit CATGTACTCTGCGTTGATACCACTG 48642 3.5824417490493734 No Hit ACGCAGAGTACATGGGAAGCAGTGG 34761 2.5601179564718817 No Hit GCGTTGATACCACTGCTTCCCATGT 32444 2.3894728856987353 No Hit TATCAACGCAGAGTACATGGGAAGC 29712 2.18826341942673 No Hit ACTCTGCGTTGATACCACTGCTTCC 28203 2.0771268584441325 No Hit GGTATCAACGCAGAGTACATGGGAA 25874 1.9055979979216213 No Hit GTATCAACGCAGAGTACATGGGAAG 25021 1.8427752765709546 No Hit GTATCAACGCAGAGTACTTTTTTTT 19259 1.4184088985843897 No Hit GCTTCCCATGTACTCTGCGTTGATA 17185 1.265660570235876 No Hit GGTATCAACGCAGAGTACTTTTTTT 16969 1.2497523547473135 No Hit TATCAACGCAGAGTACTTTTTTTTT 16482 1.213885220752267 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 15383 1.1329448095396266 No Hit GTGGTATCAACGCAGAGTACATGGG 13804 1.0166528083524025 No Hit CAGTGGTATCAACGCAGAGTACATG 13711 1.0098034377948266 No Hit ACATGGGAAGCAGTGGTATCAACGC 13178 0.9705484430938829 No Hit CATGGGAAGCAGTGGTATCAACGCA 12943 0.9532408938354928 No Hit ATACCACTGCTTCCCATGTACTCTG 12551 0.9243704286895829 No Hit GGGAAGCAGTGGTATCAACGCAGAG 12398 0.9131021093851842 No Hit GCAGTGGTATCAACGCAGAGTACAT 11411 0.8404104024999467 No Hit GTACTCTGCGTTGATACCACTGCTT 11213 0.8258278716354308 No Hit GCAGAGTACATGGGAAGCAGTGGTA 10779 0.7938641423667449 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 10689 0.7872357192465103 No Hit GATACCACTGCTTCCCATGTACTCT 10640 0.7836269111032715 No Hit CTGCTTCCCATGTACTCTGCGTTGA 10447 0.7694126259676577 No Hit TACCACTGCTTCCCATGTACTCTGC 10246 0.7546091476658008 No Hit GTTGATACCACTGCTTCCCATGTAC 9808 0.7223508218139931 No Hit AAGCAGTGGTATCAACGCAGAGTAC 9646 0.7104196601975712 No Hit CTTCCCATGTACTCTGCGTTGATAC 9416 0.6934803566680833 No Hit CCACTGCTTCCCATGTACTCTGCGT 9269 0.682653932238367 No Hit CCATGTACTCTGCGTTGATACCACT 8501 0.626091388279033 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 8392 0.6180636313889714 No Hit ATGGGAAGCAGTGGTATCAACGCAG 8243 0.6070899086676943 No Hit GTACATGGTAAGCAGTGGTATCAAC 7193 0.5297583055982925 No Hit TTCCCATGTACTCTGCGTTGATACC 6772 0.49875201522475143 No Hit ACCATGTACTCTGCGTTGATACCAC 6294 0.4635477235417285 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 5980 0.44042189176668833 No Hit TGATACCACTGCTTCCCATGTACTC 5897 0.43430901266691657 No Hit ATCAACGCAGAGTACATGGGAAGCA 5187 0.3820181191628449 No Hit GGAAGCAGTGGTATCAACGCAGAGT 3859 0.2842120535664967 No Hit CCCCATGTACTCTGCGTTGATACCA 3800 0.27986675396545413 No Hit TCCATGTACTCTGCGTTGATACCAC 3495 0.2574037645024374 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 3476 0.25600443073261014 No Hit GGTAAGCAGTGGTATCAACGCAGAG 3379 0.24886046359191305 No Hit GTACATGGGTAAGCAGTGGTATCAA 3101 0.22838600106496665 No Hit GTGGTATCAACGCAGAGTACTTTTT 2901 0.2136561719088901 No Hit GAGTACATGGTAAGCAGTGGTATCA 2900 0.2135825227631097 No Hit AAAAAGTACTCTGCGTTGATACCAC 2839 0.20908992487050637 No Hit CTGCGTTGATACCACTGCTTCCCAT 2825 0.20805883682958104 No Hit CTCTGCGTTGATACCACTGCTTCCC 2651 0.19524388546379445 No Hit TGGGAAGCAGTGGTATCAACGCAGA 2579 0.1899411469676069 No Hit TCAACGCAGAGTACATGGGAAGCAG 2544 0.1873634268652935 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 2512 0.18500665420032125 No Hit GTACATGGAAAGCAGTGGTATCAAC 2358 0.17366468575014232 No Hit ATCAACGCAGAGTACTTTTTTTTTT 2342 0.1724862994176562 No Hit AGTGGTATCAACGCAGAGTACATGG 2238 0.1648267882564964 No Hit AAAGTACTCTGCGTTGATACCACTG 2177 0.16033419036389307 No Hit GCTTACCATGTACTCTGCGTTGATA 2115 0.15576794332550933 No Hit AACGCAGAGTACATGGGAAGCAGTG 2078 0.15304292493163518 No Hit TCCCATGTACTCTGCGTTGATACCA 2054 0.151275345432906 No Hit ACTCTGCGTTGATACCACTGCTTAC 1999 0.14722464241498495 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 1882 0.13860769235868017 No Hit ACGCAGAGTACATGGTAAGCAGTGG 1874 0.1380184991924371 No Hit GTACATGGAAGCAGTGGTATCAACG 1822 0.13418874361185723 No Hit GAGTACATGGAAAGCAGTGGTATCA 1818 0.1338941470287357 No Hit ACTGCTTCCCATGTACTCTGCGTTG 1810 0.13330495386249264 No Hit GCGTTGATACCACTGCTTACCATGT 1798 0.13242116411312801 No Hit ATGGTAAGCAGTGGTATCAACGCAG 1564 0.11518726400051849 No Hit CAACGCAGAGTACATGGGAAGCAGT 1538 0.11327238621022853 No Hit CTGCTTACCATGTACTCTGCGTTGA 1513 0.11143115756571896 No Hit TATCAACGCAGAGTACATGGTAAGC 1488 0.10958992892120942 No Hit TTGATACCACTGCTTCCCATGTACT 1448 0.1066439630899941 No Hit CGCAGAGTACATGGGAAGCAGTGGT 1392 0.10251961092629268 No Hit CGTTGATACCACTGCTTCCCATGTA 1372 0.10104662801068502 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 2.20947437341148E-4 0.0 0.0 0.0 0.0 9 2.20947437341148E-4 0.0 0.0 7.364914578038266E-5 0.0 10 2.9459658312153066E-4 0.0 0.0 2.20947437341148E-4 0.0 11 2.9459658312153066E-4 0.0 0.0 3.6824572890191334E-4 0.0 12 2.9459658312153066E-4 0.0 0.0 8.101406035842093E-4 0.0 13 2.9459658312153066E-4 0.0 0.0 8.83789749364592E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGACGC 80 4.311005E-10 16.62387 7 GTACAAA 235 0.0 16.189983 1 AATACTT 45 6.7650294E-4 14.776775 5 TACATAA 125 0.0 14.43902 2 CGCAAAA 245 0.0 14.345965 2 GGACGCA 90 3.6405254E-8 13.721292 8 TGGGACG 90 3.6405254E-8 13.721292 6 ACGCAAA 375 0.0 13.443083 1 CTCCAAA 50 0.0014856334 13.316261 1 TTGCTCT 50 0.0015001439 13.299097 4 TTATACT 50 0.0015001439 13.299097 4 GTACATA 180 0.0 13.210576 1 GAACAAA 820 0.0 12.759483 1 CATTTAC 105 1.9588697E-8 12.682154 1 CGCAAAG 120 7.403287E-10 12.665808 2 TTTACTC 85 3.945268E-6 12.293283 3 CAAGTAC 70 1.0913954E-4 12.213458 3 AAAGCAT 55 0.0030696613 12.090089 15 GCAAAGA 120 1.0017175E-8 11.874195 3 TACAAGA 80 2.8677263E-5 11.874194 2 >>END_MODULE