FastQCFastQC Report
Wed 25 May 2016
SRR1295006_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295006_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3911791
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC148710.3801583469055479No Hit
CCCATGTACTCTGCGTTGATACCAC136380.3486382580255438No Hit
GAGTACATGGGAAGCAGTGGTATCA70580.18042886238042882No Hit
GTATCAACGCAGAGTACTTTTTTTT59480.15205311326704316No Hit
CATGTACTCTGCGTTGATACCACTG53120.13579457593721136No Hit
GGTATCAACGCAGAGTACTTTTTTT49760.1272051599893757No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN47050.12027738700763921No Hit
ATTCCATTCCATTCCATTCCATTCC45060.11519020315758179No Hit
GCGTTGATACCACTGCTTCCCATGT41910.10713762570648586No Hit
GCTTCCCATGTACTCTGCGTTGATA39790.10171811326320859No Hit
GTATCAACGCAGAGTACATGGGAAG39690.10146247588380872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT500.001494043313.3073254
TGTACGG550.003058708512.0967945
AGACCGT600.005885116811.0816226
ACTCGGA1158.855386E-710.73650911
TATTCCG1605.4023985E-1010.6927015
ACCGGCC803.777026E-410.6863988
TACACCG909.475756E-510.5606935
TCGGAAC1002.4094434E-510.44664613
CTAGGAC3950.010.1056633
GGAACGA2002.5465852E-119.972692515
ACGATTC1054.1201478E-59.94982318
TAGACTG5700.09.8381195
TCTAGAC4150.09.6186443
CTCGGAA1401.0640324E-69.49719312
GTCTAGG4150.09.1659021
TTAGACT4400.09.0731764
CTAGACT4550.08.9829674
TAGACAG5850.08.935975
TAGGACT5000.08.9349184
GTCCAGG7600.08.8839891
Produced by FastQC (version 0.11.5)