FastQCFastQC Report
Wed 25 May 2016
SRR1295006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295006_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3911791
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC151230.3866004088664246No Hit
GTACATGGGAAGCAGTGGTATCAAC149730.3827658481754266No Hit
GTATCAACGCAGAGTACTTTTTTTT142550.36441108433451586No Hit
GGTATCAACGCAGAGTACTTTTTTT128070.32739479179741454No Hit
TATCAACGCAGAGTACTTTTTTTTT98190.25101034283273316No Hit
GAGTACATGGGAAGCAGTGGTATCA77750.19875806248339953No Hit
CATGTACTCTGCGTTGATACCACTG58570.14972681311450434No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56680.14489526664384678No Hit
ACGCAGAGTACATGGGAAGCAGTGG47670.12186233875991842No Hit
GCTTCCCATGTACTCTGCGTTGATA44750.1143977272814422No Hit
GCGTTGATACCACTGCTTCCCATGT44190.11296615795680291No Hit
GTATCAACGCAGAGTACATGGGAAG43020.10997520061782441No Hit
ATTCCATTCCATTCCATTCCATTCC42860.10956618081078462No Hit
GGTATCAACGCAGAGTACATGGGAA40180.10271509904286807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC802.8711782E-511.87410513
TATTCGA802.8715538E-511.87395416
TCGTGCA600.00588165711.0824999
TATTCCG1909.094947E-1210.4993435
TACCCCG750.002650482310.13275
AAGACCG750.002650482310.13275
TCTGGAC4200.09.7255823
GTCCTAT4050.09.3954591
TCTACAC5900.09.1773923
TCCTGCG1054.4981373E-49.0470532
CCCTTAC5050.09.0419671
CGAAATG950.00182621038.99920719
GGACTGT5600.08.9903946
CTGAGCG850.0074435668.9405029
GTATTAG6300.08.9089091
ATTAGAC3750.08.8661123
TAGACTG5900.08.8553785
GACAGTC2659.094947E-128.6032367
TTACACT6300.08.59474
GGACCGT1000.00290842528.54935556
Produced by FastQC (version 0.11.5)