FastQCFastQC Report
Wed 25 May 2016
SRR1295005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3872970
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC750981.9390287040694867No Hit
CCCATGTACTCTGCGTTGATACCAC700461.8085861754674062No Hit
GAGTACATGGGAAGCAGTGGTATCA348500.8998262315483982No Hit
CATGTACTCTGCGTTGATACCACTG264310.682447837189547No Hit
GCGTTGATACCACTGCTTCCCATGT221790.5726612909472575No Hit
GTATCAACGCAGAGTACATGGGAAG192860.49796409473866304No Hit
ACGCAGAGTACATGGGAAGCAGTGG187670.48456352618274867No Hit
TATCAACGCAGAGTACATGGGAAGC172910.44645323872893417No Hit
GGTATCAACGCAGAGTACATGGGAA159050.41066674929059616No Hit
ACTCTGCGTTGATACCACTGCTTCC156870.4050379940975531No Hit
GCTTCCCATGTACTCTGCGTTGATA150660.3890037877907652No Hit
ATTCCATTCCATTCCATTCCATTCC122510.3163205498622504No Hit
GTATCAACGCAGAGTACTTTTTTTT103180.26641053248540525No Hit
GTGGTATCAACGCAGAGTACATGGG94710.24454101116197646No Hit
GAATGGAATGGAATGGAATGGAATG86770.22403994866988386No Hit
CAGTGGTATCAACGCAGAGTACATG80400.20759262271590023No Hit
GGTATCAACGCAGAGTACTTTTTTT77940.20124090814026444No Hit
ATACCACTGCTTCCCATGTACTCTG74860.1932883549317449No Hit
GTACTCTGCGTTGATACCACTGCTT71620.1849226820760295No Hit
ACATGGGAAGCAGTGGTATCAACGC71580.18481940216423057No Hit
GGGAAGCAGTGGTATCAACGCAGAG70300.181514444986664No Hit
GCAGTGGTATCAACGCAGAGTACAT69840.180326726000976No Hit
CATGGGAAGCAGTGGTATCAACGCA67890.17529183030077694No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA67290.17374263162379258No Hit
TATCAACGCAGAGTACTTTTTTTTT65660.16953397521798516No Hit
CTGCTTCCCATGTACTCTGCGTTGA62600.1616330619653651No Hit
TACCACTGCTTCCCATGTACTCTGC59600.15388706858044343No Hit
GATACCACTGCTTCCCATGTACTCT59360.1532673891096497No Hit
GTTGATACCACTGCTTCCCATGTAC58140.15011735179978156No Hit
GTACATGGTAAGCAGTGGTATCAAC58140.15011735179978156No Hit
CCATGTACTCTGCGTTGATACCACT57350.1480775735417522No Hit
CTTCCCATGTACTCTGCGTTGATAC55610.14358489737849764No Hit
GCAGAGTACATGGGAAGCAGTGGTA55100.14226807850306095No Hit
AAGCAGTGGTATCAACGCAGAGTAC53820.1389631213254944No Hit
CCACTGCTTCCCATGTACTCTGCGT51940.13410896547094348No Hit
TTCCCATGTACTCTGCGTTGATACC48110.12421991391619351No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN46690.1205534770473306No Hit
ATGGGAAGCAGTGGTATCAACGCAG45750.11812639912005514No Hit
ACCATGTACTCTGCGTTGATACCAC43150.11141320485312306No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA39520.10204055285736786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC250.006016190619.0095423
ACACCGT1251.4169927E-912.1576076
GGCGTAG1209.848009E-911.8897411
ACCGCAA3150.011.75837211
CGTACAC600.00585640511.08890153
CGCAAAA3300.010.9446062
GACGGTG1752.0008883E-1110.8559897
CGTAGGT1252.2276618E-710.6453453
GTAAGAC4050.010.5608593
TAAGACG1451.4320904E-810.489114
GTACAAA6900.010.2010541
TATTCCG2800.010.1843435
TACGCCC1502.4256224E-810.1394744
ACACCGA750.002653423210.1313396
CTAGGAC2201.8189894E-129.9368083
TCACCGC3950.09.8597129
GTCTAAG2350.09.7141711
CGCTCTG2550.09.6917675
TAGACTG4250.09.6172165
CTCACCG4050.09.6162628
Produced by FastQC (version 0.11.5)