##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295005_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3872970
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.855389274897558	33.0	33.0	33.0	27.0	33.0
2	30.726207277618986	33.0	33.0	33.0	27.0	33.0
3	30.798472231904714	33.0	33.0	33.0	27.0	33.0
4	34.22303374412918	37.0	37.0	37.0	27.0	37.0
5	34.56379548511865	37.0	37.0	37.0	27.0	37.0
6	34.565692478898626	37.0	37.0	37.0	27.0	37.0
7	34.45808617159441	37.0	37.0	37.0	27.0	37.0
8	34.36105727645709	37.0	37.0	37.0	27.0	37.0
9	34.2747449631678	37.0	37.0	37.0	27.0	37.0
10	34.23039553624221	37.0	37.0	37.0	27.0	37.0
11	34.421506750633235	37.0	37.0	37.0	27.0	37.0
12	34.35967848963457	37.0	37.0	37.0	27.0	37.0
13	34.21849717400341	37.0	37.0	37.0	27.0	37.0
14	35.57565227719296	40.0	37.0	40.0	27.0	40.0
15	35.69708853928639	40.0	37.0	40.0	27.0	40.0
16	35.76566794991957	37.0	37.0	40.0	27.0	40.0
17	35.71896064260761	37.0	37.0	40.0	27.0	40.0
18	35.66157083581851	37.0	37.0	40.0	27.0	40.0
19	35.70464811243051	40.0	37.0	40.0	27.0	40.0
20	35.762217884465926	40.0	37.0	40.0	27.0	40.0
21	35.76277249759229	40.0	37.0	40.0	27.0	40.0
22	35.69529353441932	37.0	37.0	40.0	27.0	40.0
23	35.64004213820402	37.0	37.0	40.0	27.0	40.0
24	35.7223069117499	37.0	37.0	40.0	27.0	40.0
25	34.59561163654766	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4124759498283517
1101	2	0.41389626743499264
1101	3	0.417533962426873
1101	4	0.4656623174432184
1101	5	0.30271570674181447
1101	6	0.3786189201643282
1101	7	0.30920053314454776
1101	8	0.4632522616548016
1101	9	0.5004554055455301
1101	10	0.6636870004416267
1101	11	0.5011144461238857
1101	12	0.5097622167839972
1101	13	0.5070914689962791
1101	14	0.993387000640233
1101	15	0.8363304266994902
1101	16	0.8351492934756379
1101	17	0.7237386400867294
1101	18	0.8123889222876954
1101	19	0.7140174812324105
1101	20	0.6881084938634316
1101	21	0.75017779098701
1101	22	0.7848648707966532
1101	23	0.8425438824234845
1101	24	0.7619639638372959
1101	25	0.882949135620521
1102	1	0.461555987784795
1102	2	0.38168285986821715
1102	3	0.38109702975480175
1102	4	0.5045092875468526
1102	5	0.22095520860367657
1102	6	0.2612156371013583
1102	7	0.29773370392151577
1102	8	0.3617907845871713
1102	9	0.3796900699814074
1102	10	0.5387911343647644
1102	11	0.45425252785340575
1102	12	0.4689390805488074
1102	13	0.499766499734001
1102	14	0.8049290822298261
1102	15	0.7592586749461319
1102	16	0.7555784517252704
1102	17	0.6554554670826747
1102	18	0.7822672412223355
1102	19	0.5701751464250009
1102	20	0.6296797057918653
1102	21	0.6457369794844112
1102	22	0.6745151468766792
1102	23	0.7840382093032616
1102	24	0.7057540714412909
1102	25	0.9211182322572071
1103	1	0.4520368047043988
1103	2	0.3711030151141834
1103	3	0.2959866846181676
1103	4	0.4750154465884364
1103	5	0.3485161245656698
1103	6	0.2961837875381903
1103	7	0.2431952344020658
1103	8	0.4417793735981945
1103	9	0.4429187310287688
1103	10	0.5524764684302923
1103	11	0.35404781769452853
1103	12	0.5294862076968059
1103	13	0.48577718390762925
1103	14	0.8933117640378114
1103	15	0.8540484409056432
1103	16	0.768297144408443
1103	17	0.8023633091875269
1103	18	0.7630908613995189
1103	19	0.6821790544673902
1103	20	0.7514884961099995
1103	21	0.8381083470827662
1103	22	0.7359440212388293
1103	23	0.7709221815935621
1103	24	0.7845082082886279
1103	25	0.9238274173999272
1104	1	0.37894352115053564
1104	2	0.3053882803376631
1104	3	0.25938469051769175
1104	4	0.29199821184403874
1104	5	0.13446700844382065
1104	6	0.17144420461134757
1104	7	0.2103935195126425
1104	8	0.39631398362906367
1104	9	0.3860724350657776
1104	10	0.5273226796237793
1104	11	0.34621186180972785
1104	12	0.47263000594923454
1104	13	0.3519792712785659
1104	14	0.6854036340061995
1104	15	0.6121219254977106
1104	16	0.575041831035648
1104	17	0.6217125963972805
1104	18	0.6939401401443348
1104	19	0.5444153459721832
1104	20	0.6069375241244046
1104	21	0.47942312926296893
1104	22	0.552099803569476
1104	23	0.5273993339914753
1104	24	0.6221520387798805
1104	25	0.7366569573380701
1105	1	0.2632276618723175
1105	2	0.19364432286251798
1105	3	0.19546333287167528
1105	4	0.25092816294125697
1105	5	0.08677115952242076
1105	6	0.14555169143963553
1105	7	0.11011266347691162
1105	8	0.23936993197015255
1105	9	0.2600474010500946
1105	10	0.3411624377780811
1105	11	0.22013951189512682
1105	12	0.34192756979496863
1105	13	0.3881202628490712
1105	14	0.6772099310106725
1105	15	0.48139269819146335
1105	16	0.35954682166722307
1105	17	0.39570912850213347
1105	18	0.49145367300606324
1105	19	0.47527876516373624
1105	20	0.5127626894863582
1105	21	0.49403593172826277
1105	22	0.40912807934481776
1105	23	0.5929277672140287
1105	24	0.6074959163840958
1105	25	0.5457437229509665
1106	1	0.05771088130382651
1106	2	-0.17233078578042793
1106	3	-0.16529678089372624
1106	4	0.058474198042610226
1106	5	-0.4672093559630994
1106	6	-0.4669629797581081
1106	7	-0.8934787331600589
1106	8	-0.30875479250515525
1106	9	-0.09253586023302773
1106	10	-0.34954834603760787
1106	11	-0.40751529160026223
1106	12	-0.8418497464416888
1106	13	-0.3220074928390275
1106	14	-0.40524708952295185
1106	15	-0.18019844716413047
1106	16	-0.1942582547699203
1106	17	-0.24463643395364443
1106	18	-0.6044189492772958
1106	19	-0.6975267895093751
1106	20	-0.1911536271369556
1106	21	-0.014575607009561509
1106	22	0.03070652898069426
1106	23	-0.661310316453509
1106	24	-0.03536942398626053
1106	25	0.11855860766156212
1107	1	-1.0693601872976792
1107	2	-1.1091526272247059
1107	3	-0.9403117413050488
1107	4	-0.9338012164821521
1107	5	-1.0191451892769194
1107	6	-1.0385517498256718
1107	7	-1.0594020448596524
1107	8	-0.9918716603353772
1107	9	-1.092083266623419
1107	10	-1.0099150206571395
1107	11	-1.0745664585245436
1107	12	-1.0253230599465297
1107	13	-1.02434958700875
1107	14	-1.2478563047947873
1107	15	-1.092724470839272
1107	16	-1.0730953288769598
1107	17	-1.202322773713668
1107	18	-1.2922010198128575
1107	19	-1.3144037374732491
1107	20	-1.393002326575278
1107	21	-1.35030471024848
1107	22	-1.3711634492489466
1107	23	-1.0404981948244512
1107	24	-0.9771870852976718
1107	25	-0.9510856138749872
1108	1	-0.027868896096109097
1108	2	-0.1934250286591741
1108	3	0.021507004868379198
1108	4	-0.1143663144423499
1108	5	-0.3797502857064927
1108	6	0.055369635124705496
1108	7	0.050294349213963585
1108	8	0.16314581109289605
1108	9	-0.1021232034785271
1108	10	-0.3936065508788289
1108	11	0.03177125533101588
1108	12	0.16131174601824938
1108	13	0.11688566448123083
1108	14	-0.21528759731944547
1108	15	0.09220575478569515
1108	16	-0.8088021699199288
1108	17	-0.7033052780720723
1108	18	-0.5974678830975009
1108	19	-0.02492220640409215
1108	20	-0.8669586241155542
1108	21	-0.13633216707957274
1108	22	-0.2761476273535308
1108	23	-0.20363985878152846
1108	24	-0.38054461970654074
1108	25	-0.9820684565713833
1109	1	0.16335614269535625
1109	2	0.0388388055840565
1109	3	0.1404526548261984
1109	4	0.1369940897526476
1109	5	0.09099945482974192
1109	6	0.04890276986452591
1109	7	0.024762548413683305
1109	8	0.20146947242982094
1109	9	0.13438504100741255
1109	10	0.21832675644282773
1109	11	0.10445512956255243
1109	12	0.15412447058947976
1109	13	0.18659136983779945
1109	14	0.4074415885546898
1109	15	0.301085678290697
1109	16	0.23720466966527454
1109	17	0.3325753784759016
1109	18	0.3215100984346648
1109	19	0.3284660422061165
1109	20	0.2605164966777096
1109	21	0.32548673610898504
1109	22	0.20742243682710892
1109	23	0.3764296022092424
1109	24	0.3791079448181591
1109	25	0.3489918730822481
1110	1	-0.11319953436072439
1110	2	-0.20169463843164337
1110	3	-0.03253346968151405
1110	4	-1.205406662728791
1110	5	-0.45601504232759993
1110	6	-0.2096588326033455
1110	7	-0.0548705399449787
1110	8	-0.07331853696429391
1110	9	-0.009282010370078808
1110	10	-0.1736396793887991
1110	11	-0.19289821865005052
1110	12	-0.060165347095953337
1110	13	-0.07199895642813203
1110	14	0.0028055213842890225
1110	15	-0.04547476742878587
1110	16	-0.059520012063501326
1110	17	-0.01986759850161235
1110	18	0.039585020255920256
1110	19	-0.060951336071276785
1110	20	-0.0762047402470003
1110	21	0.06399535753132568
1110	22	-0.4685410119346898
1110	23	-0.12480757280727062
1110	24	0.009433279274730921
1110	25	-0.01044783640936231
1111	1	-0.14859813121508836
1111	2	-0.261721310115945
1111	3	-0.18211030096563618
1111	4	-0.05996002802058342
1111	5	-0.15067510457720346
1111	6	-0.05124143566610684
1111	7	-0.03988897637881195
1111	8	0.040308347063245265
1111	9	-0.05229633587317295
1111	10	-0.04959115360339439
1111	11	-0.03589054941654979
1111	12	-0.10293897741001956
1111	13	-0.14987458171181345
1111	14	-0.25939177119854406
1111	15	-0.12117003157974437
1111	16	-0.1803812429572389
1111	17	-0.10676663601725522
1111	18	-0.21472574881477868
1111	19	-0.12092455400446767
1111	20	-0.12292117239001499
1111	21	-0.1784085118252534
1111	22	-0.13740907714090156
1111	23	0.017238514137964955
1111	24	-0.04929920861717818
1111	25	-0.014619477528434288
1112	1	0.03109752749076833
1112	2	-0.2980854350476996
1112	3	-0.021235151290756704
1112	4	-0.02741849057000678
1112	5	0.040263982378554886
1112	6	-0.028363877350415123
1112	7	0.0885146639422274
1112	8	0.1869807197983704
1112	9	0.08674404306026418
1112	10	0.3405700820179476
1112	11	0.11306984888046401
1112	12	0.15220269309394752
1112	13	0.05246290119338681
1112	14	-0.11273247783904594
1112	15	-0.030278193232277317
1112	16	-0.030890595575982616
1112	17	-0.1137341262876248
1112	18	-0.030654708160618327
1112	19	0.034576295493344844
1112	20	-0.7642110003611151
1112	21	-1.0790460219681322
1112	22	-1.5444607232693883
1112	23	-0.44950088810708877
1112	24	-0.176098564277261
1112	25	-1.2250356887071234
1113	1	-0.1811483014708557
1113	2	-0.22841793266943
1113	3	-0.26118058822873635
1113	4	-0.5084201325044475
1113	5	-0.19427955512908568
1113	6	-0.1437144161986197
1113	7	-0.13968856790847184
1113	8	-0.05555574748295555
1113	9	-0.1383059551409218
1113	10	-0.001356719789832539
1113	11	-0.14739976854998815
1113	12	-0.19648278622528892
1113	13	-0.2440520133393207
1113	14	-0.5830150273904877
1113	15	-0.36034247579126344
1113	16	-0.35011355482073725
1113	17	-0.386031761111731
1113	18	-0.8052793751587402
1113	19	-1.086334423731742
1113	20	-1.275951657288232
1113	21	-1.1127787363912631
1113	22	-0.5617032465404463
1113	23	-1.952567277343455
1113	24	-0.9797590489192132
1113	25	-0.4612063425497155
1114	1	-0.31014615188669126
1114	2	-0.444181860530211
1114	3	-0.3311765473566801
1114	4	-0.5533656805884917
1114	5	-0.5617657465971888
1114	6	-0.4568205886140433
1114	7	-0.43075318125293194
1114	8	-0.20623533961450846
1114	9	-1.0093072725850405
1114	10	-0.24140898484174755
1114	11	-0.3528616362317365
1114	12	-0.36704902756853386
1114	13	-0.4642808718229219
1114	14	-0.5897736688319952
1114	15	-1.2484935338406942
1114	16	-1.074325035557365
1114	17	-1.5416897142735664
1114	18	-1.6980231805418171
1114	19	-1.4455374931967953
1114	20	-0.7639938048486314
1114	21	-0.6348725538570221
1114	22	-0.46500873979645263
1114	23	-0.17943711266626394
1114	24	-0.773335227346017
1114	25	-0.5702028748557169
1115	1	-0.6751404759015713
1115	2	-0.4421450318395692
1115	3	-0.4849392046347276
1115	4	-0.5892531712836231
1115	5	-0.3625648523541045
1115	6	-0.3627495153601288
1115	7	-0.31554910785808943
1115	8	-0.12344232737243743
1115	9	-0.5951075208525509
1115	10	-0.1446533633098923
1115	11	-0.3709810712979831
1115	12	-0.4712422664749525
1115	13	-0.5374804987559472
1115	14	-0.4031245215987127
1115	15	-1.4452185743442598
1115	16	-0.9450862251523304
1115	17	-0.6959050860995859
1115	18	-2.1546833984542673
1115	19	-1.1634810463287835
1115	20	-0.9084301158136654
1115	21	-0.8626926117673506
1115	22	-0.5645973375313673
1115	23	-0.2651574508268979
1115	24	-0.30764343272863215
1115	25	-0.501401913702864
1116	1	-1.3568139038991234
1116	2	-0.6562298893207483
1116	3	-1.5286286048212574
1116	4	-0.7262179468576662
1116	5	-0.754099440384806
1116	6	-0.3262607742380439
1116	7	-0.9379085285847424
1116	8	-2.774699871585284
1116	9	-1.3000107260009344
1116	10	-0.67318790887078
1116	11	-0.473651845118475
1116	12	-0.542739871307873
1116	13	-1.6007273178507475
1116	14	-1.533359275354826
1116	15	-1.5017917652000605
1116	16	-0.8704570536806457
1116	17	-1.5669661040451288
1116	18	-2.3739209805509276
1116	19	-1.2796711445663078
1116	20	-0.7969377517184668
1116	21	-0.6392825800621438
1116	22	-0.41771106405445124
1116	23	-0.4385836089535644
1116	24	-0.4378502458180549
1116	25	-0.4338230805088301
1201	1	0.38319166019478956
1201	2	0.2886194462960674
1201	3	0.33288638515143987
1201	4	0.3605255180435307
1201	5	0.24620867267150714
1201	6	0.06664809718366627
1201	7	0.08309226535615011
1201	8	0.293419063362542
1201	9	0.185990697018795
1201	10	0.42717310646246887
1201	11	0.34453265502957464
1201	12	0.37121759862378667
1201	13	0.4048434464692079
1201	14	0.7874969117983
1201	15	0.6091259523717341
1201	16	0.5924585044153261
1201	17	0.5639037981114186
1201	18	0.45836695208991074
1201	19	0.49635981262053974
1201	20	0.5575420217697697
1201	21	0.5743083429809062
1201	22	0.5212987116138592
1201	23	0.6798741337596965
1201	24	0.6140888354882179
1201	25	0.7751750788932412
1202	1	0.5018988601621253
1202	2	0.47712672789398525
1202	3	0.4837285693740263
1202	4	0.5824498529688071
1202	5	0.27106315953844273
1202	6	0.08057228409568751
1202	7	0.11746355754849702
1202	8	0.24104055584329132
1202	9	0.22592470352532246
1202	10	0.4882633101704883
1202	11	0.36217197449400373
1202	12	0.32828482455086316
1202	13	0.3571397651659751
1202	14	0.8394657352317338
1202	15	0.5314255359728222
1202	16	0.5116070326803523
1202	17	0.5618731456521076
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2210	19	0.19722093176748956
2210	20	0.2865898270050167
2210	21	0.3328979506760277
2210	22	0.5010040026897045
2210	23	0.41310269350042006
2210	24	0.41300252751148747
2210	25	0.4233389325123085
2211	1	0.17024550856733356
2211	2	0.053026292400080166
2211	3	0.11935049615598103
2211	4	0.20070983886981253
2211	5	0.19763448651480076
2211	6	0.2530741799998495
2211	7	0.25363166473395893
2211	8	0.26457601304973366
2211	9	0.2026073270975246
2211	10	0.3741597516723516
2211	11	0.2163317631841437
2211	12	0.24837381693362914
2211	13	0.2227447082382028
2211	14	0.17645777795968343
2211	15	0.13818718548080966
2211	16	0.13553170853484886
2211	17	0.2004835388444448
2211	18	0.28832375046554404
2211	19	0.25604635619300353
2211	20	0.2622563153325004
2211	21	0.13381433134754417
2211	22	0.13993933407435577
2211	23	0.31791279489277
2211	24	0.313719255291943
2211	25	0.29037075953200286
2212	1	0.014166055225491192
2212	2	0.10650908162509154
2212	3	0.1294018492483282
2212	4	0.020109510646911133
2212	5	0.11029608666723334
2212	6	0.12471860505368682
2212	7	0.18583897572575836
2212	8	0.23787730804335894
2212	9	0.04818057314391666
2212	10	0.2347229664710042
2212	11	0.1356505252647935
2212	12	0.14661168665775648
2212	13	0.09972312919663295
2212	14	0.2076377331230148
2212	15	0.10348742433333058
2212	16	0.015229483563039992
2212	17	0.1390470793148353
2212	18	0.1372234971830153
2212	19	0.19861888293137042
2212	20	0.1586833833979
2212	21	0.1890655440462652
2212	22	-0.3370742998921301
2212	23	0.24475678656801136
2212	24	0.22359557975347855
2212	25	0.39840506049973357
2213	1	-0.11198986693061741
2213	2	-0.7423672102196726
2213	3	-0.20917964572278436
2213	4	-0.1430590733929833
2213	5	0.09003008886178776
2213	6	0.008791619510041926
2213	7	8.224455577590106E-4
2213	8	0.19154553756944637
2213	9	-0.12486191287971593
2213	10	-1.4347747839347065
2213	11	-0.42516247728367773
2213	12	-0.10423283743556766
2213	13	-0.12186826493631031
2213	14	0.04704025603028583
2213	15	-0.05563868433610253
2213	16	-0.029730561132922162
2213	17	-0.10076118730795258
2213	18	0.06851493357353178
2213	19	-0.05058029464394309
2213	20	-0.06060647632353522
2213	21	-0.0511636411401426
2213	22	0.11555659500962179
2213	23	-0.7504361719236883
2213	24	-1.2665756877014402
2213	25	-1.289886064581502
2214	1	-0.09082667678076817
2214	2	-0.22971234059921386
2214	3	-0.01600079585930203
2214	4	-0.0628514230816819
2214	5	0.010820669806498984
2214	6	-0.1737088014812187
2214	7	-0.03842443881655555
2214	8	0.009564567535889523
2214	9	-0.4419645406781427
2214	10	-0.07071508098614032
2214	11	-0.24588528153842049
2214	12	-0.22800201576514212
2214	13	-0.16625324025170585
2214	14	-0.1917267099176172
2214	15	-0.20104555932666557
2214	16	-0.276155387190947
2214	17	-0.17551801694759916
2214	18	-0.1827836357529833
2214	19	-0.9039482828167067
2214	20	-0.39209850420070325
2214	21	-0.38991137504579143
2214	22	-0.6511873714597343
2214	23	-1.8939636025530362
2214	24	-1.3387515912067585
2214	25	-1.6136575656214944
2215	1	-0.5530274495173018
2215	2	-0.3689606545031694
2215	3	-1.2509056116969006
2215	4	-0.9430341959225288
2215	5	-0.30571341710091815
2215	6	-0.3383641516400928
2215	7	-0.31512367002415687
2215	8	-0.19091232450007567
2215	9	-0.42946419918327194
2215	10	-1.5584994305797366
2215	11	-0.6774275275245074
2215	12	-0.5929378713505571
2215	13	-0.5731402513726067
2215	14	-1.0307070981661255
2215	15	-1.159131156500429
2215	16	-0.7486182318082655
2215	17	-0.5812023736980549
2215	18	-0.4676849870837998
2215	19	-0.947148324121919
2215	20	-1.4323754405358784
2215	21	-0.7128561780133325
2215	22	-0.4608063096637096
2215	23	-0.7109269989280875
2215	24	-1.8538177525772213
2215	25	-0.9307431254501282
2216	1	-0.556028013321761
2216	2	-0.5178113725266904
2216	3	-1.3675553053403
2216	4	-0.7529233336630554
2216	5	-0.37217576964182086
2216	6	-0.36973036093591105
2216	7	-0.32369505975263735
2216	8	-0.4588140950294104
2216	9	-0.24549208364027209
2216	10	-0.36343347943155635
2216	11	-0.3380741166054477
2216	12	-0.31555747075457674
2216	13	-1.0390546907364993
2216	14	-0.6181374962177699
2216	15	-1.0987258247290228
2216	16	-0.865815509321834
2216	17	-1.7905557915147625
2216	18	-0.8848299070352041
2216	19	-0.472715317626367
2216	20	-0.5003587901591402
2216	21	-0.2982913548857056
2216	22	-0.37736883489712625
2216	23	-0.22740160813599175
2216	24	-0.3726236984435829
2216	25	-0.16050522328777106
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5187.0
3	534.0
4	610.0
5	546.0
6	446.0
7	345.0
8	592.0
9	1416.0
10	2700.0
11	4907.0
12	8114.0
13	12295.0
14	17468.0
15	23375.0
16	27676.0
17	29125.0
18	28741.0
19	27975.0
20	28144.0
21	29342.0
22	30388.0
23	30479.0
24	31524.0
25	32044.0
26	33223.0
27	36904.0
28	40226.0
29	46581.0
30	55709.0
31	70642.0
32	94296.0
33	134045.0
34	193616.0
35	310635.0
36	568438.0
37	1914682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.023091400481476	21.377469850994313	12.300234830435642	22.299203918088576
2	13.796802621187362	23.46382790930249	38.98886859373743	23.75050087577272
3	18.844554790258577	29.67215443331356	28.75058117407383	22.73270960235404
4	11.81338019056886	18.60880074115863	36.24868666897847	33.32913239929404
5	12.612920902885758	39.70788046116018	33.5167385282665	14.162460107687563
6	30.96291194789242	37.399288115544884	17.609127933780982	14.02867200278172
7	23.796626744157468	34.47467988477275	24.038076217110497	17.690617153959288
8	24.532234161998733	37.198779553405146	21.882593989836167	16.38639229475995
9	24.60619478873451	17.38151168826598	21.622140555221744	36.39015296777776
10	15.885829930177067	31.408880814480106	31.626216984208323	21.079072271134507
11	30.126292700078576	28.860235095414854	20.211075145100903	20.802397059405667
12	20.91239975978313	31.1091193661007	30.768510269892086	17.209970604224083
13	26.499966876723448	25.566615049560703	24.97849574779937	22.95492232591648
14	20.128627480203395	25.467940935685224	27.328294009217547	27.075137574893837
15	26.72739512530322	28.56614329356636	22.703472848899303	22.002988732231117
16	20.7836541185657	32.209931729161376	27.077109282241587	19.929304870031334
17	21.153749628077257	31.327097967684765	28.694997477393564	18.824154926844415
18	21.180692560159486	29.992768313050544	29.543239407648514	19.28329971914146
19	20.721152875902526	29.36019299463024	30.22798577378707	19.69066835568016
20	23.270452943715014	28.204328149530312	27.824365426548646	20.700853480206028
21	22.139883540338523	30.675144707869006	27.52169938898391	19.663272362808556
22	20.456844031845474	29.748367351532544	27.573952035154107	22.22083658146787
23	18.9363851304324	31.2352875122189	28.59960144003204	21.22872591731666
24	20.51375477286284	30.869123604693744	27.402281658103224	21.214839964340197
25	22.007330765538416	27.496658718319278	25.238741530352293	25.25726898579001
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14157.0
1	14157.0
2	13596.5
3	13036.0
4	13036.0
5	13036.0
6	16336.5
7	19637.0
8	19637.0
9	19637.0
10	22962.0
11	26287.0
12	26287.0
13	26287.0
14	34362.0
15	42437.0
16	42437.0
17	42437.0
18	60348.0
19	78259.0
20	78259.0
21	78259.0
22	106860.0
23	135461.0
24	135461.0
25	135461.0
26	169870.5
27	204280.0
28	204280.0
29	204280.0
30	241019.5
31	277759.0
32	277759.0
33	277759.0
34	315591.5
35	353424.0
36	353424.0
37	353424.0
38	391960.0
39	430496.0
40	430496.0
41	430496.0
42	427767.0
43	425038.0
44	425038.0
45	425038.0
46	537073.0
47	649108.0
48	649108.0
49	649108.0
50	567583.5
51	486059.0
52	486059.0
53	486059.0
54	387415.0
55	288771.0
56	288771.0
57	288771.0
58	234100.0
59	179429.0
60	179429.0
61	179429.0
62	150301.5
63	121174.0
64	121174.0
65	121174.0
66	95981.5
67	70789.0
68	70789.0
69	70789.0
70	53381.0
71	35973.0
72	35973.0
73	35973.0
74	24712.5
75	13452.0
76	13452.0
77	13452.0
78	9317.5
79	5183.0
80	5183.0
81	5183.0
82	3495.0
83	1807.0
84	1807.0
85	1807.0
86	1225.0
87	643.0
88	643.0
89	643.0
90	440.5
91	238.0
92	238.0
93	238.0
94	147.0
95	56.0
96	56.0
97	56.0
98	36.5
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3178955685171845
2	0.18696246033405886
3	0.20524300472247398
4	0.22592480706021476
5	0.25192552485560177
6	0.1997433494191796
7	0.19532813318977427
8	0.2190308729476345
9	0.21936653266098113
10	0.230056003532173
11	0.20400364578088648
12	0.20989060075342694
13	0.22251656997084923
14	0.21084593993756728
15	0.20103434831666656
16	0.20457168529578076
17	0.20449422536193154
18	0.20746352282615152
19	0.20635326377431273
20	0.2027901068172488
21	0.20059540869152098
22	0.20477824511937864
23	0.20800574236309605
24	0.22398830871398437
25	0.21779151400604704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3872970.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.662454427222386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.55442824581976	47.34398982671386
2	11.23054973373021	12.727010249602852
3	2.7189029683003194	4.621791466000697
4	0.9541717688650451	2.1626285747623117
5	0.4218231096506243	1.1950766363463867
6	0.2329265600800857	0.791891435725652
7	0.14988057213251885	0.5944820761589407
8	0.09717310065977063	0.4404853110148917
9	0.0777753924275926	0.3966250166089023
>10	0.4582495128113106	5.037464912644109
>50	0.05266765141594248	2.116503036546815
>100	0.043133703662094784	4.876457469825364
>500	0.004066421554683687	1.559898893543762
>1k	0.002633932138493597	3.0380795665298663
>5k	0.0010166053867870025	3.8503130751036356
>10k+	6.007213649195924E-4	9.247302452871953
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	75098	1.9390287040694867	No Hit
CCCATGTACTCTGCGTTGATACCAC	70046	1.8085861754674062	No Hit
GAGTACATGGGAAGCAGTGGTATCA	34850	0.8998262315483982	No Hit
CATGTACTCTGCGTTGATACCACTG	26431	0.682447837189547	No Hit
GCGTTGATACCACTGCTTCCCATGT	22179	0.5726612909472575	No Hit
GTATCAACGCAGAGTACATGGGAAG	19286	0.49796409473866304	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	18767	0.48456352618274867	No Hit
TATCAACGCAGAGTACATGGGAAGC	17291	0.44645323872893417	No Hit
GGTATCAACGCAGAGTACATGGGAA	15905	0.41066674929059616	No Hit
ACTCTGCGTTGATACCACTGCTTCC	15687	0.4050379940975531	No Hit
GCTTCCCATGTACTCTGCGTTGATA	15066	0.3890037877907652	No Hit
ATTCCATTCCATTCCATTCCATTCC	12251	0.3163205498622504	No Hit
GTATCAACGCAGAGTACTTTTTTTT	10318	0.26641053248540525	No Hit
GTGGTATCAACGCAGAGTACATGGG	9471	0.24454101116197646	No Hit
GAATGGAATGGAATGGAATGGAATG	8677	0.22403994866988386	No Hit
CAGTGGTATCAACGCAGAGTACATG	8040	0.20759262271590023	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7794	0.20124090814026444	No Hit
ATACCACTGCTTCCCATGTACTCTG	7486	0.1932883549317449	No Hit
GTACTCTGCGTTGATACCACTGCTT	7162	0.1849226820760295	No Hit
ACATGGGAAGCAGTGGTATCAACGC	7158	0.18481940216423057	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	7030	0.181514444986664	No Hit
GCAGTGGTATCAACGCAGAGTACAT	6984	0.180326726000976	No Hit
CATGGGAAGCAGTGGTATCAACGCA	6789	0.17529183030077694	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6729	0.17374263162379258	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6566	0.16953397521798516	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	6260	0.1616330619653651	No Hit
TACCACTGCTTCCCATGTACTCTGC	5960	0.15388706858044343	No Hit
GATACCACTGCTTCCCATGTACTCT	5936	0.1532673891096497	No Hit
GTTGATACCACTGCTTCCCATGTAC	5814	0.15011735179978156	No Hit
GTACATGGTAAGCAGTGGTATCAAC	5814	0.15011735179978156	No Hit
CCATGTACTCTGCGTTGATACCACT	5735	0.1480775735417522	No Hit
CTTCCCATGTACTCTGCGTTGATAC	5561	0.14358489737849764	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	5510	0.14226807850306095	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	5382	0.1389631213254944	No Hit
CCACTGCTTCCCATGTACTCTGCGT	5194	0.13410896547094348	No Hit
TTCCCATGTACTCTGCGTTGATACC	4811	0.12421991391619351	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	4669	0.1205534770473306	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	4575	0.11812639912005514	No Hit
ACCATGTACTCTGCGTTGATACCAC	4315	0.11141320485312306	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3952	0.10204055285736786	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.581997794973883E-5	0.0
3	0.0	0.0	0.0	2.581997794973883E-5	0.0
4	0.0	0.0	0.0	2.581997794973883E-5	0.0
5	0.0	0.0	0.0	2.581997794973883E-5	0.0
6	0.0	0.0	0.0	2.581997794973883E-5	0.0
7	0.0	0.0	0.0	2.581997794973883E-5	0.0
8	0.0	0.0	0.0	5.163995589947766E-5	0.0
9	0.0	0.0	0.0	1.0327991179895532E-4	0.0
10	0.0	0.0	0.0	1.8073984564817181E-4	0.0
11	5.163995589947766E-5	0.0	0.0	2.3237980154764947E-4	0.0
12	5.163995589947766E-5	0.0	0.0	2.581997794973883E-4	0.0
13	5.163995589947766E-5	0.0	0.0	2.8401975744712715E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAC	25	0.0060161906	19.009542	3
ACACCGT	125	1.4169927E-9	12.157607	6
GGCGTAG	120	9.848009E-9	11.889741	1
ACCGCAA	315	0.0	11.758372	11
CGTACAC	60	0.005856405	11.0889015	3
CGCAAAA	330	0.0	10.944606	2
GACGGTG	175	2.0008883E-11	10.855989	7
CGTAGGT	125	2.2276618E-7	10.645345	3
GTAAGAC	405	0.0	10.560859	3
TAAGACG	145	1.4320904E-8	10.48911	4
GTACAAA	690	0.0	10.201054	1
TATTCCG	280	0.0	10.184343	5
TACGCCC	150	2.4256224E-8	10.139474	4
ACACCGA	75	0.0026534232	10.131339	6
CTAGGAC	220	1.8189894E-12	9.936808	3
TCACCGC	395	0.0	9.859712	9
GTCTAAG	235	0.0	9.714171	1
CGCTCTG	255	0.0	9.691767	5
TAGACTG	425	0.0	9.617216	5
CTCACCG	405	0.0	9.616262	8
>>END_MODULE