FastQCFastQC Report
Wed 25 May 2016
SRR1295005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3872970
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC790002.0397782580293677No Hit
GTACATGGGAAGCAGTGGTATCAAC737141.9032938545870484No Hit
GAGTACATGGGAAGCAGTGGTATCA376750.9727676692564104No Hit
CATGTACTCTGCGTTGATACCACTG296320.765097586606661No Hit
GTATCAACGCAGAGTACTTTTTTTT279960.7228561026808883No Hit
GCGTTGATACCACTGCTTCCCATGT238890.616813453241311No Hit
ACGCAGAGTACATGGGAAGCAGTGG228020.5887471372099449No Hit
GGTATCAACGCAGAGTACTTTTTTT227840.5882823776068495No Hit
GTATCAACGCAGAGTACATGGGAAG200020.5164511989506761No Hit
TATCAACGCAGAGTACTTTTTTTTT199640.5154700397885861No Hit
TATCAACGCAGAGTACATGGGAAGC189290.48874636261060633No Hit
ACTCTGCGTTGATACCACTGCTTCC179800.46424320353630416No Hit
GGTATCAACGCAGAGTACATGGGAA171490.4427868018600712No Hit
GCTTCCCATGTACTCTGCGTTGATA167880.43346578982021544No Hit
ATTCCATTCCATTCCATTCCATTCC119300.30803233694038423No Hit
ACGCAGAGTACTTTTTTTTTTTTTT116990.3020679220339946No Hit
GTGGTATCAACGCAGAGTACATGGG98880.25530794196701756No Hit
CAGTGGTATCAACGCAGAGTACATG98190.25352636348848556No Hit
GAATGGAATGGAATGGAATGGAATG92260.23821511656429045No Hit
ACATGGGAAGCAGTGGTATCAACGC91030.2350392592764726No Hit
CATGGGAAGCAGTGGTATCAACGCA84950.21934071268303137No Hit
GCAGTGGTATCAACGCAGAGTACAT81260.20981314081957775No Hit
GGGAAGCAGTGGTATCAACGCAGAG80720.20841886201029186No Hit
ATACCACTGCTTCCCATGTACTCTG80380.20754098276000071No Hit
GTACTTTTTTTTTTTTTTTTTTTTT78120.20170566774335977No Hit
GTACTCTGCGTTGATACCACTGCTT71400.18435464256113526No Hit
CCATGTACTCTGCGTTGATACCACT68370.17653118924236438No Hit
CTGCTTCCCATGTACTCTGCGTTGA67980.17552421010232458No Hit
TACCACTGCTTCCCATGTACTCTGC66240.17103153393907003No Hit
GATACCACTGCTTCCCATGTACTCT65790.16986963493133175No Hit
CTTCCCATGTACTCTGCGTTGATAC65050.1679589565630511No Hit
GCAGAGTACATGGGAAGCAGTGGTA62560.16152978205356613No Hit
AAGCAGTGGTATCAACGCAGAGTAC61930.15990312344273258No Hit
GTTGATACCACTGCTTCCCATGTAC57860.14939439241718888No Hit
GAGTACTTTTTTTTTTTTTTTTTTT57770.14916201261564122No Hit
GTACATGGTAAGCAGTGGTATCAAC57540.14856815312279725No Hit
CCACTGCTTCCCATGTACTCTGCGT57180.14763863391660664No Hit
ATGGGAAGCAGTGGTATCAACGCAG54030.1395053408624389No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA52740.13617456370692257No Hit
ACCATGTACTCTGCGTTGATACCAC48960.1264146120419213No Hit
TTCCCATGTACTCTGCGTTGATACC46670.12050183709143111No Hit
ATCAACGCAGAGTACATGGGAAGCA40370.10423525098309566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG555.91961E-717.2715196
GGCGTAG1350.014.7946971
ACGCCGA707.2735274E-613.5706547
GCCGAGT707.2735274E-613.5706549
CGCCGAG802.8706265E-511.8743228
GCGTAGG1751.8189894E-1211.3994962
TTCGAGT1751.8189894E-1211.39920118
CTGTGCG1104.978574E-711.2266329
CGCAGAA1451.2205419E-911.1374392
ACGCAAA2700.010.9198951
CGTAGGT1752.0008883E-1110.8563823
TCTGTCG2650.010.3956338
CGCAAAA2200.010.3631782
TGGGACG1409.71213E-810.1778596
TAAGACG1803.947207E-1010.0270764
CAACGCC951.6464238E-49.99935
GGAACGA3350.09.92467915
CTTAGAC2300.09.912353
TCGGAAC1356.4555206E-79.8512913
ACGATTC1451.6209015E-79.82689818