FastQCFastQC Report
Wed 25 May 2016
SRR1295004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3242161
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC148830.45904567971794125No Hit
CCCATGTACTCTGCGTTGATACCAC129930.4007512273449715No Hit
GAGTACATGGGAAGCAGTGGTATCA68430.2110629299408635No Hit
GTATCAACGCAGAGTACTTTTTTTT54350.16763510510428076No Hit
CATGTACTCTGCGTTGATACCACTG50640.15619212000884594No Hit
GGTATCAACGCAGAGTACTTTTTTT43450.1340155532066421No Hit
GCGTTGATACCACTGCTTCCCATGT41810.12895719860919924No Hit
GTATCAACGCAGAGTACATGGGAAG40990.12642802131047778No Hit
GCTTCCCATGTACTCTGCGTTGATA40300.1242998111444805No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN39160.12078363782674581No Hit
ACGCAGAGTACATGGGAAGCAGTGG37830.116681435622722No Hit
ATTCCATTCCATTCCATTCCATTCC37330.11513925434301381No Hit
GGTATCAACGCAGAGTACATGGGAA36740.11331948043295814No Hit
TATCAACGCAGAGTACTTTTTTTTT35050.10810690770754444No Hit
TATCAACGCAGAGTACATGGGAAGC33230.1024933678494066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGA500.00150132713.2986269
ACAGTCG600.00588250811.0821898
ACCGTTT700.001493444310.8560218
CCTACAC3350.010.7808393
GTCTTAG4000.09.9881761
GCATGCG900.00111525259.4990199
TAGCCTG4900.09.3120725
TGGACAG4800.09.1099865
CAGGACT4300.09.064354
GAACAGT4300.09.0572036
GTATAGA6100.09.0447181
ACTAGAC3500.08.9610663
GTCCTAG4150.08.9395041
TAGACAG5850.08.9373335
CCAGGAC4900.08.9222743
TAATACT6850.08.8819994
CTTAGCC3550.08.8348543
TCTGGAC3550.08.8348543
GTATTAG4100.08.8165071
TAACACG1304.3363565E-58.7752434
Produced by FastQC (version 0.11.5)